論文 - 堀口　崇

MitoK_ATP opener, diazoxide, reduces neuronal damage after middle cerebral artery occlusion in the rat

Shimizu Katsuyoshi, Lacza Zsombor, Rajapakse Nishadi, Horiguchi Takashi, Snipes James, Busija David W

American Journal of Physiology - Heart and Circulatory Physiology 283 （ 3 52-3 ）  2002年

ISSN  0363-6135

　概要を見る

<p>We investigated effects of diazoxide, a selective opener of mitochondrial ATP-sensitive K<sup>+</sup> (mitoK<sub>ATP</sub>) channels, against brain damage after middle cerebral artery occlusion (MCAO) in male Wistar rats. Diazoxide (0.4 or 2 mM in 30 μl saline) or saline (sham) was infused into the right lateral ventricle 15 min before MCAO. Neurological score was improved 24 h later in the animals treated with 2 mM diazoxide (13.8 ± 0.7, n = 13) compared with sham treatment (9.5 ± 0.2, n = 6, P < 0.01). The total percent infarct volume (MCAO vs. contralateral side) of sham treatment animals was 43.6 ± 3.6% (n = 12). Treatment with 2 mM diazoxide reduced the infarct volume to 20.9 ± 4.8% (n = 13, P < 0.05). Effects of diazoxide were prominent in the cerebral cortex. The protective effect of diazoxide was completely prevented by the pretreatment with 5-hydroxydecanoate (100 mM in 10 μl saline), a selective blocker of mitoK<sub>ATP</sub> channels (n = 6). These results indicate that selective opening of the mitoK<sub>ATP</sub> channel has neuroprotective effects against ischemia-reperfusion injury in the rat brain.</p>

The effect of intraischemic hypothermia on the expression of c-Fos, c-Jun and DNA binding activity of AP-1 after focal cerebral ischemia in rat brain

Akaji K, Suga S, Fujino T, Takayama H, Ochiai M, Mayanagi K, Inamasu J, Horiguchi T, Sato S, Kawase T.

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 21(Sup-1)   S428 2001年

研究論文（国際会議プロシーディングス）, 共著

Intra-ischemic hypothermia attenuates intercellular adhesion molecule-1 (ICAM-1) and migration of neutrophil

Inamasu J., Suga S., Sato S., Horiguchi T., Akaji K., Mayanagi K., Kawase T.

Neurological Research 23 （ 1 ） 105 - 111 2001年

ISSN  0161-6412

　概要を見る

<p>Adhesion of neutrophil to the endothelium and subsequent transmigration has been reported to contribute to progression of focal ischemia. Hypothermia has been known to attenuate ischemic insult through various mechanisms of action. The authors evaluated the effect of hypothermia on expression of intercellular adhesion molecule-1 (ICAM-1) protein and on transmigration of neutrophil with immunohistochemical method. Transient focal ischemia model in rats was employed, and animals received 2 h of either normothermic or hypothermic ischemia. To confirm the effectiveness of hypothermia on neuroprotection, cortical infarct area was compared between the two groups. Our results demonstrated that hypothermia reduced both the number of microvessels expressing ICAM-1 and that of neutrophils migrating into ischemic tissue. Comparison of cortical infarct area showed persistent protective effect. This study indicates that reduction of ICAM-1 expression and subsequent reduction of migrating neutrophil in hypothermia can contribute to attenuation of ischemic damage.</p>

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Interhemispheric subdural empyema in a young man [5]

Inamasu J., Horiguchi T., Saito R., Nakamura Y., Ichikizaki K., Takahashi K., Shimanouchi T.

American Journal of Emergency Medicine 19 （ 7 ） 602 - 603 2001年

ISSN  0735-6757

Delayed, but acutely progressive epidural hematoma after mild head injury [8]

Inamasu J., Nakamura Y., Saito R., Horiguchi T., Kanai R., Ichikizaki K.

American Journal of Emergency Medicine 19 （ 4 ） 324 - 325 2001年

ISSN  0735-6757

Cerebral microaneurysms found incidentally during aneurysm surgery

Inamasu J., Suga S., Horiguchi T., Akaji K., Mayanagi K., Kawase T.

Neurological Research 23 （ 4 ） 304 - 308 2001年

ISSN  0161-6412

　概要を見る

<p>Not uncommonly, cerebral microaneurysms are found incidentally during surgery for another previously diagnosed cerebral aneurysm(s). The frequency and angiographic characteristics of such incidental microaneurysms are retrospectively summarized. Seventeen patients were identified as harboring incidental microaneurysms, comprising 4.9% of the whole series. The middle cerebral artery (MCA) was the most frequent location (seven cases, 41%) of these microaneurysms. There was a tendency for MCA microaneurysms to be contiguous to a previously known, larger aneurysm at the same location. Neurosurgeons as well as interventional neuroradiologists should be aware of the possible presence of these incidental microaneurysms while treating patients with a cerebral aneurysm(s). Although the actual clinical implications of these incidental microaneurysms have not been elucidated, the few additional risks to patients already surgically exposed for the treatment of another aneurysm, along with the possible benefit of preventing their rupture and growth, would justify the surgical treatment of these microaneurysms.</p>

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重症脳損傷患者における近赤外線分光分析装置（NIRO-300）を用いた脳酸素代謝障害の評価

堀口　崇、斉藤良一、各務　宏、山本紳一郎、菊野隆明、中村芳樹、市来嵜　潔

日本救急医学会雑誌 12   1 - 10 2001年

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Effect of intraischemic hypothermia on intercellular adhesion molecule-1 and migration of neutrophils

Inamasu, J; Suga, S; Sato, S; Horiguchi, T; Akaji, K; Mayanagi, K; Kawase, T

ISCHEMIC BLOOD FLOW IN THE BRAIN 6   407 - 412 2001年

Post-ischemic hypothermia delayed neutrophil accumulation and microglial activation following transient focal ischemia in rats

Inamasu Joji, Suga Sadao, Sato Shuzo, Horiguchi Takashi, Akaji Kazunori, Mayanagi Keita, Kawase Takeshi

Journal of Neuroimmunology 109 （ 2 ） 66 - 74 2000年09月

ISSN  0165-5728

　概要を見る

<p>Following ischemia, inflammation has been demonstrated to be involved in the progression of the tissue damage. Intra-ischemic hypothermia has been shown to attenuate the adverse activities of neutrophils and microglia. We investigated whether neutrophil accumulation and/or microglial activation is attenuated in post-ischemic hypothermia following transient focal ischemia in rats. After 1 h of ischemia, the neutrophil accumulation and the microglial activation was evaluated immunohistochemically. Percent infarct area was compared at 1, 2, 3, 5, and 7 days after ischemia/reperfusion. In hypothermia, the neutrophil accumulation was delayed but not attenuated. In normothermia, the accumulation reached the peak at 2 days after ischemia. The peak shifted to 3 days in hypothermia. Similarly, the microglial activation was delayed in hypothermia. Comparison of the infarct area showed significant protection by hypothermia at 1 and 2 days after reperfusion. However, hypothermia failed to show significant protection after 3 days and later. These results show that the delayed neutrophil accumulation and the microglial activation can be responsible for the loss of persistent protection in post-ischemic hypothermia. (C) 2000 Elsevier Science B.V.</p>

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Effect of Intraischemic Hypothermia on Intracellular Adhesion Molecule-1 and Migration of Neutrophil

Inamasu J, Suga S, Sato S, Horiguchi T,Akaji K, Mayanagi K, Kawase T.

Ischemic Blood flow in the Brain    407 - 412 2000年

研究論文（研究会，シンポジウム資料等）, 共著

Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats.

Inamasu J., Suga S., Sato S., Horiguchi T., Akaji K., Mayanagi K., Kawase T.

Acta Neurochirurgica, Supplement 76   525 - 527 2000年

ISSN  0065-1419

　概要を見る

<p>Hypothermia confers potent neuroprotection against ischemic injury. Attenuation of apoptosis by hypothermia can be one of the responsible mechanisms. In this study, in situ DNA nick-end labeling (TUNEL) and immunostaining of Bax protein were performed to evaluate the effect of postischemic hypothermia on apoptotic cell death, employing rodent transient focal ischemia. Animals received 1 hour of transient focal ischemia. Brain temperature was maintained at 37.5 +/- 0.5 degrees C during ischemia. Immediately after reperfusion, animals were assigned to either a normothermic or hypothermic group. In hypothermia, animals were cooled and brain temperature was lowered to 34.5 +/- 1.0 degrees C. Prolonged hypothermia was maintained for 16 hours and animals rewarmed. In both groups, TUNEL and immunostaining of Bax was performed. In normothermia, the number of TUNEL positive cells reached the peak at 2 days after ischemia and decreased gradually. In hypothermia, the peak was shifted to 3 days after ischemia. The number of TUNEL positive cells in hypothermia was persistently below that of normothermia. Similarly, in hypothermia, immunostaining of Bax showed attenuated immunoreactivity compared with that in normothermia. In conclusion, postischemic hypothermia reduced both the number of TUNEL positive cells and immunoreactivity of Bax, which may be one of the responsible mechanisms with which hypothermia exerts neuroprotection.</p>

院外心肺機能停止患者の転帰からみた救急救命士制度運用の地域差

鈴木昌, 堀進悟, 篠澤洋太郎, 藤島清太郎, 木村裕之, 堀口崇, 中澤堅次, 小林健二 佐々木淳一, 相川直樹

日本臨床救急医学会雑誌 3   326 - 332 2000年

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SIRS,ALI患者の重症度評価指標としてのAPACHE IIIスコア,PaO2/F1O2比の検討

藤島 清太郎, 篠沢 洋太郎, 堀 進悟, 青木 克憲, 木村 裕之, 横山 雅子, 堀口 崇, 高月 誠司, 鈴木 昌, 山崎 元靖, 関根 和彦, 宮武 諭, 三村 琢也, 相川 直樹

日本救命医療研究会雑誌 （日本救命医療学会）  13   119 - 124 1999年12月

ISSN  1341-6782

　概要を見る

低酸素血症が各種の重症度評価指標値に及ぼす影響を検討するため,当院救急部経由で入院した235名のSIRS患者を,低酸素血症の有無で,更に低酸素血症を伴う患者はその原因を急性肺損傷(Acute lung injury,ALI)とそれ以外で分類し,各群の重症度評価に適する指標を各々検討した.ALI患者及びALI以外の低酸素血症病態の予後は,APACHE IIIスコア,不全臓器数では適切に評価し得ず,一方低酸素血症を呈さないSIRS病態の予後評価は両指標値のいずれでも可能であった.低酸素血症を伴うSIRS病態のうち,ALIに対してはPaO2/F1O2比が重症度評価の妥当な指標と思われたが,ALI以外では予後と相関せず,新たな指標値が必要と考えられた

Intraischemic Hypothermia Attenuates Intercellular Adhesion Molecule-1 (ICAM-1) and Migration of Neutrophil Following Transient Focal Ischemia in Rats

INAMASU Joji, SUGA Sadao, AKAJI Kazunori, HORIGUCHI Takashi, SATO Shuzo, KAWASE Takeshi

Keio journal of medicine 48   A71 1999年11月

ISSN  00229717

• CiNii Research

脳虚血におけるWater channel mRNAの発現

佐藤 周三, 赤路 和則, 稻桝 丈司, 堀口 崇, 石川 真実, 菅 貞郎, 河瀬 斌, 梅西 文範

Progress in Research on Brain Edema and ICP （脳浮腫・頭蓋内圧フォーラム）   （ 1 ） 5 - 8 1999年05月

ISSN  1344-8234

　概要を見る

脳虚血後には細胞内外の水分の移動のwater channelによる生理的制御が失われており,虚血後の細胞内外水分の移動はion channelにより移動するイオンに付随した形で受動的に行われているものと考えられた.water channelの詳細な病態生理学的な意義は不明なものの,脳虚血においては虚血後脳浮腫の進展になんらかの関与があるものと推察された

The effect of intra-ischemic hypothermia on expression of c-Fos, c-Jun and HSP after transient focal cerebral ischemia in rat brain

Akaji K, Suga S, Mayanagi K, Inamasu J, Horiguchi T, Sato S, Kawase T.

Journal of Cerebral Blood Flow and Metabolism 19(Sup-1)   S279 1999年

研究論文（国際会議プロシーディングス）, 共著

A case of traumatic shock complicated by methamphetamine intoxication.

堀口 崇

Intensive Care Med 25   758 - 760 1999年

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Mitochondria toxin(3-NP)脳内注入後のアポトーシス発生について

佐藤周三, 赤路和則, 稻枡丈司, 堀口崇, 菅貞郎, 河瀬斌, 近藤健男, 村上謙介, ChanP.H.

脳循環代謝 11   162 - 163 1999年

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ラット脳局所虚血ー再潅流モデルにおける虚血後長期低体温の脳保護効果についての考察

稲桝丈司、菅　貞郎、堀口　崇、佐藤周三、赤路和則、河瀬　斌

脳循環代謝 11   298 - 299 1999年

妊娠経過中にショックを呈した1例

山崎元靖，堀進悟，藤島清太郎，木村裕之，横山雅子，高月誠司，堀口崇，鈴木昌，関根和彦，宮武諭，三村琢也，相川直樹

日本救急医学会関東地方会雑誌 20   126 - 127 1999年

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