論文 - 南宮　湖

Effects Of Histone Deacetylase Inhibitor Trichostatin A During Influenza A Virus Infection In Vivo And In Vitro

K Yagi, M Ishii, H Fujii, K Mizoguchi, H Namkoong, T Asami, Y Funatsu, ...

D31. RESPIRATORY TRACT INFECTION AND INFLAMMATION: FROM ANIMAL MODELS TO … 2013年

Mesenchymal Stem Cell-Conditioned Medium Modulates Murine Macrophage Cytokine Expression Induced By Tlr7/8 Ligand Stimulation And Influenza A Virus Infection

T Asami, M Ishii, H Fujii, H Namkoong, K Yagi, Y Funatsu, Y Kimizuka, ...

D28. MAKING REPARATIONS: MESENCHYMAL STEM CELLS AND FIBROCYTES IN LUNG … 2013年

インフルエンザウイルス感染後の急性呼吸不全に併発した敗血症性ショックに対してポリミキシン B 固定化線維カラムによる直接血液灌流療法が奏効した 1 例

荒井大輔， 福永興壱， 藤井健太郎， 南宮湖， 原口水葉， 舩津洋平， ...

日本内科学会雑誌 102 (2), 429-432 2013年

The Efficacy Of Amikacin In Combination With Standard Regimen To Patients With Nontuberculous Mycobacterial Infection

H Tanaka, Y Funatsu, H Namkoong, K Yagi, T Asami, H Fujiwara, ...

C106. ADVANCES IN NON-TUBERCULOUS MYCOBACTERIAL DISEASE, A5117-A5117 2013年

Retrospective observation of mycobacterium avium complex lung disease followed without treatment

Y Kimizuka, T Asami, M Ishii, S Tasaka, H Namkoong, H Fujiwara, ...

European Respiratory Journal 42 (Suppl 57) 2013年

Research Article Modulation of Murine Macrophage TLR7/8-Mediated Cytokine Expression by Mesenchymal Stem Cell-Conditioned Medium

T Asami, M Ishii, H Fujii, H Namkoong, S Tasaka, K Matsushita, K Ishii, ...

2013年

Penetration and pharmacokinetics of peramivir in upper and lower airway epithelia and plasma

Y Funatsu, N Hasegawa, H Namkoong, T Asami, H Fujiwara, Y Kimizuka, ...

European Respiratory Journal 42 (Suppl 57) 2013年

P8 Prostaglandin D2 receptor CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis

M Ishii, K Asano, H Namkoong, S Tasaka, J Miyata, S Kagawa, ...

International Journal of Antimicrobial Agents, S43 2013年

P298 Clinical and radiological features of Mycobacterium avium complex lung disease observed without chemotherapy

Y Kimizuka, T Asami, M Ishii, S Tasaka, H Namkoong, H Fujiwara, ...

International Journal of Antimicrobial Agents, S136 2013年

P24 Pharmacokinetics of peramivir in upper and lower airway epithelia and plasma

Y Funatsu, N Hasegawa, H Namkoong, T Asami, H Fujiwara, Y Kimizuka, ...

International Journal of Antimicrobial Agents, S48-S49 2013年

P1-2-4 上気道及び下気道におけるペラミビルの薬物動態 (合併症・安全対策-1, 一般演題ポスター, 第 36 回日本呼吸器内視鏡学会学術集会)

舩津洋平， 長谷川直樹， 南宮湖， 浅見貴弘， 藤原宏， 君塚善文， 田坂定智， ...

気管支学 35 (Special), S194 2013年

Modulation of murine macrophage TLR7/8-mediated cytokine expression by mesenchymal stem cell-conditioned medium

T Asami, M Ishii, H Fujii, H Namkoong, S Tasaka, K Matsushita, K Ishii, ...

Mediators of Inflammation 2013 2013   264260 - 264260 2013年

査読有り

　概要を見る

Increasing evidence suggests that mesenchymal stem cells (MSCs) play anti-inflammatory roles during innate immune responses. However, little is known about the effect of MSCs or their secretions on the ligand response of Toll-like receptor (TLR) 7 and TLR8, receptors that recognize viral single-stranded RNA (ssRNA). Macrophages play a critical role in the innate immune response to ssRNA virus infection; therefore, we investigated the effect of MSC-conditioned medium on cytokine expression in macrophages following stimulation with TLR7/8 ligands. After stimulation with TLR7/8 ligand, bone marrow-derived macrophages cultured with MSCs or in MSC-conditioned medium expressed lower levels of tumor necrosis factor (TNF) α and interleukin (IL) 6 and higher levels of IL-10 compared to macrophages cultured without MSCs or in control medium, respectively. The modulations of cytokine expression were associated with prostaglandin E2 (PGE2) secreted by the MSCs. PGE2 enhanced extracellular signal-related kinase (ERK) signaling and suppressed nuclear factor- κ B (NF- κ B) signaling. Enhanced ERK signaling contributed to enhanced IL-10 production, and suppression of NF- κ B signaling contributed to the low production of TNF- α . Collectively, these results indicate that MSCs and MSC-conditioned medium modulate the cytokine expression profile in macrophages following TLR7/8-mediated stimulation, which suggests that MSCs play an immunomodulatory role during ssRNA virus infection.

• Access to Document (DOI)

Cytokine profile of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema

S Tasaka, K Mizoguchi, Y Funatsu, H Namkoong, W Yamasawa, M Ishii, ...

Respirology 17 (5), 814-820 17 （ 5 ） 814 - 20 2012年07月

査読有り

　概要を見る

BACKGROUND AND OBJECTIVE: Combined pulmonary fibrosis and emphysema (CPFE) is characterized by upper lobe emphysema together with lower lobe fibrosis. The aim of this study was to examine whether cytokine levels in the alveolar space are associated with emphysematous changes superimposed on pulmonary fibrosis. METHODS: Consecutive patients (n = 102), diagnosed with pulmonary fibrosis were retrospectively evaluated. Cytokine levels and differential cell counts in bronchoalveolar lavage (BAL) fluid, pulmonary function, computed tomography (CT) scores and levels of serum markers were compared between patients with or without emphysema. RESULTS: Among the 102 patients (14 females, mean age 68 years), 38 (37%) had evidence of upper lobe emphysema on computed tomography (CT). Levels of epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) and interleukin (IL)-8/CXCL8 in BAL fluid were significantly higher in patients with emphysema. Vital capacity (VC, % predicted) was greater, and ratio of forced expiratory volume in 1 s/forced vital capacity and diffusing capacity of carbon monoxide (DL(CO))/alveolar volume (V(A)) were lower in patients with emphysema. CXCL8 and CXCL5 levels were associated with percentage or absolute numbers of neutrophils in BAL fluid. In addition, CXCL8 levels were inversely correlated with VC and DL(CO)/V(A), and positively correlated with composite physiological index (CPI) and the extent of areas of low attenuation on CT. CONCLUSIONS: Increased CXC chemokine levels in the airspaces may be associated with emphysematous lung changes in patients with pulmonary fibrosis.

• Access to Document (DOI)

CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis

M Ishii, K Asano, H Namkoong, S Tasaka, K Mizoguchi, T Asami, ...

The Journal of Immunology 188 (11), 5655-5664 188 （ 11 ） 5655 - 64 2012年06月

査読有り

　概要を見る

Although arachidonic acid cascade has been shown to be involved in sepsis, little is known about the role of PGD(2) and its newly found receptor, chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2), on the septic response. Severe sepsis is associated with the failure of neutrophil migration. To investigate whether CRTH2 influences neutrophil recruitment and the lethality during sepsis, sepsis was induced by cecal ligation and puncture (CLP) surgery in mice. CRTH2 knockout (CRTH2(-/-)) mice were highly resistant to CLP-induced sepsis, which was associated with lower bacterial load and lower production of TNF-α, IL-6, and CCL3. IL-10, an anti-inflammatory cytokine, was higher in CRTH2(-/-) mice, blunting CLP-induced lethality in CRTH2(-/-) mice. Neutrophil accumulation in the peritoneum was more pronounced after CLP in CRTH2(-/-) mice, which was associated with higher CXCR2 levels in circulating neutrophils. Furthermore, sepsis caused a decrease in the level of acetylation of histone H3, an activation mark, at the CXCR2 promoter in wild-type neutrophils, suggesting that CXCR2 expression levels are epigenetically regulated. Finally, both pharmacological depletion of neutrophils and inhibition of CXCR2 abrogated the survival benefit in CRTH2(-/-) mice. These results demonstrate that genetic ablation of CRTH2 improved impaired neutrophil migration and survival during severe sepsis, which was mechanistically associated with epigenetic-mediated CXCR2 expression. Thus, CRTH2 is a potential therapeutic target for polymicrobial sepsis.

• Access to Document (DOI)

Clarithromycin Modulates Cytokine Expression Profiles In Murine Macrophages And Dendritic Cells During Influenza A Virus Infection

M Wakatabe, M Ishii, H Namkoong, H Fujii, T Asami, Y Funatsu, ...

D36. INFLUENCES AND OUTCOMES OF RESPIRATORY VIRAL INFECTIONS, A5718-A5718 2012年

Clinical Characteristics Of Late-Onset Idiopathic Pneumonia Syndrome With Severe Restrictive Lung Deficit After Hematopoietic Stem Cell Transplantation

H Namkoong, M Ishii, S Tasaka, N Hasegawa, H Sugiura, M Sakurai, ...

C47. TOPICS IN TRANSPLANT MEDICINE, A4473-A4473 2012年

Cytokine profiles of bronchoalveolar lavage fluid in patients with combined pulmonary fibrosis and emphysema

S Tasaka, K Mizoguchi, Y Funatsu, H Namkoong, W Yamasawa, M Ishii, ...

European Respiratory Journal 40 (Suppl 56) 2012年

Evaluation of cytokines and chemokines in bronchoalveolar lavage fluid of patients with collagen vascular disease-associated interstitial pneumonitis

Y Tokuda, S Tasaka, H Namkoong, T Asami, Y Funatsu, Y Kimizuka, ...

D104. CONNECTIVE TISSUE DISEASE RELATED-INTERSTITIAL LUNG DISEASE: CLINICAL … 2012年

Modulatory Roles Of Mesenchymal Stem Cell-Conditioned Media On Cytokine Expressions In Murine Macrophages And Dendritic Cells During Influenza A Virus Infection

T Asami, M Ishii, H Fujii, H Namkoong, Y Funatsu, Y Kimizuka, S Tasaka, ...

D36. INFLUENCES AND OUTCOMES OF RESPIRATORY VIRAL INFECTIONS, A5713-A5713 2012年

A clustering based schema matching scheme for improving matching correctness of web service interfaces

Byoungoh Kim, Namkoong Ho, Dongman Lee, Soon J. Hyun

Proceedings - 2011 IEEE International Conference on Services Computing, SCC 2011    488 - 495 2011年

　概要を見る

An interface adapter is used to allow a client application to transparently work with a service available in the local environment by converting the interface of an originally requested service into that of the available service. To generate adapters for users, interface matching is the first job to find out all the possible method matching pairs between a source interface and a target interface. Existing method matching schemes compare the schemas of source and target interfaces but they have low recall and precision because they only consider signatures and names of interfaces. For solving these problems, we propose a cluster-based schema matching scheme. By clustering methods in terms of parameter similarity, the proposed scheme can match methods more precisely than existing schema matching schemes. Then, we detect incorrect matches based on the analysis of incorrect method matching pairs. © 2011 IEEE.

• Access to Document (DOI)