橋口 明典 (ハシグチ アキノリ)

Hashiguchi, Akinori

写真a

所属(所属キャンパス)

医学部 電子顕微鏡研究室 (信濃町)

職名

専任講師

外部リンク

経歴 【 表示 / 非表示

  • 1995年04月
    -
    1997年03月

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学医学部助手(病理学)

  • 1997年04月
    -
    2007年03月

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学助手(医学部病理学)

  • 2007年04月
    -
    2013年11月

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学助教(医学部病理学)

  • 2013年12月
    -
    継続中

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学専任講師(医学部病理学)

学歴 【 表示 / 非表示

  • 1989年04月
    -
    1995年03月

    慶應義塾, 医学部

    日本, 大学, 卒業

学位 【 表示 / 非表示

  • 博士(医学), 慶應義塾, 論文, 2010年11月

    Using immunofluorescent digital slide technology to quantify protein expression in archival paraffin-embedded tissue sections

 

研究分野 【 表示 / 非表示

  • 人体病理学

研究キーワード 【 表示 / 非表示

  • デジタル病理学

  • 腎病理

 

論文 【 表示 / 非表示

  • Diabetic condition induces hypertrophy and vacuolization in glomerular parietal epithelial cells

    Kawaguchi T., Hasegawa K., Yasuda I., Muraoka H., Umino H., Tokuyama H., Hashiguchi A., Wakino S., Itoh H.

    Scientific Reports (Scientific Reports)  11 ( 1 )  2021年12月

    研究論文(学術雑誌), 共著, 査読有り

     概要を見る

    Diabetic nephropathy (DN) is accompanied by characteristic changes in the glomerulus, but little is known about the effect of diabetes on parietal epithelial cells (PECs). In this study, a descriptive analysis of PECs was undertaken in diabetic db/db mice and in diabetic patients. PEC hypertrophy was significantly more prominent in diabetic mice than in nondiabetic mice, and this was evident even at the early stage. Additionally, the number of vacuoles in PECs was markedly increased in diabetic mice, suggesting the presence of cellular injury in PECs in DN. Although rare, binuclear cells were observed in mice with early diabetes. In cultured PECs, a high glucose condition, compared with normal glucose condition, induced cellular hypertrophy and apoptosis. Flow cytometry showed that some PECs in the G0 phase reentered the cell cycle but got arrested in the S phase. Finally, in human diabetic subjects, hypertrophy and vacuolization were observed in the PECs. Our data showed that PECs undergo substantial changes in DN and may participate in rearrangement for differentiation into podocytes.

  • Malignancy-associated membranous nephropathy with PLA2R double-positive for glomeruli and carcinoma

    Yasuda I., Tokuyama H., Hashiguchi A., Hasegawa K., Uchiyama K., Ryuzaki M., Yasuda M., Mizuno R., Ishidoya S., Wakino S., Itoh H.

    CEN case reports (CEN case reports)  10 ( 2 ) 281 - 286 2021年05月

    研究論文(学術雑誌), 共著, 査読有り

     概要を見る

    Phospholipase A2 receptor (PLA2R) is the most common primary target antigen of idiopathic membranous nephropathy (MN) although PLA2R antibodies are also reported to be present in malignancy-associated MN. However, a case of PLA2R-positive MN secondary to PLA2R-positive carcinoma has not been reported. A 26-year-old Japanese woman presented with general fatigue, fever, and nonproductive cough. Computed tomography demonstrated a left kidney mass with pathologic diagnosis of Xp11.2 translocation renal cell carcinoma (RCC). After the second time of administration with Sunitinib, the patients exhibited significant proteinuria and hypoalbuminemia. Renal biopsy revealed a diagnosis of diffuse MN secondary to RCC. Immunofluorescence staining showed granular patterns positive for immunoglobulin (Ig) G, IgA, and C3c. PLA2R and IgG1-3 were positive, while IgG4 was negative. For the treatment of severe nephrotic syndrome, we attempted steroid therapy without any clinical improvement. Open nephrectomy was performed and surprisingly, RCC was stained for PLA2R with polarity for the basal side. At outpatient follow-up, 4 months after the operation, urinary protein had still persisted, although serum albumin was slightly increased. We report a case of PLA2R-positive MN secondary to PLA2R-positive RCC.

  • A cross-sectional study in patients with IgA nephropathy of correlations between clinical data and pathological findings at the time of renal biopsy: a Japanese prospective cohort study

    Kamano C., Shimizu A., Joh K., Hashiguchi A., Hisano S., Katafuchi R., Kawamura T.

    Clinical and Experimental Nephrology (Clinical and Experimental Nephrology)  2021年

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  13421751

     概要を見る

    Background: The correlations between clinical data and pathological findings at the time of renal biopsy were investigated in IgA nephropathy patients. Methods: 771 patients diagnosed with IgA nephropathy by renal biopsy were enrolled. The correlations between clinical variables including eGFR, daily proteinuria, mean arterial pressure (MAP), serum uric acid (UA) values, and pathological parameters were examined. These patients were further divided into three groups: children (< 19 years old), young adults (19–60 years), and elderly patients (> 60 years). Results: Daily proteinuria was moderately correlated with all pathological parameters (Rs = 0.23–0.49). The mesangial score, the percentage of glomeruli that contained endocapillary hypercellularity, cellular/fibrocellular crescents or tuft necrosis, and segmental glomerulosclerosis (GS) affected daily proteinuria most on multiple linear regression analysis (MLRA). eGFR, MAP, and serum UA levels were mainly correlated with the degree of GS and interstitial lesions. In children, the degree of cellular/fibrocellular crescents or tuft necrosis was correlated with not only daily proteinuria, but also decreased eGFR (Rs = 0.51, − 0.24). Endocapillary hypercellularity was the only independent variable related to daily proteinuria on MLRA. Conclusion: In all age cohorts of IgA nephropathy patients, daily proteinuria was correlated with all histological parameters, including both acute and chronic glomerular lesions, and the mesangial score. Independent variables for daily proteinuria were the meangial score, acute histological lesions, and segmental GS on MLRA, whereas the remaining independent variable in the pediatric group was endocapillary hypercellurality. The clinical pathological correlation at the time of biopsy varied depending on the age group.

  • Association of glomerular DNA damage and DNA methylation with one-year eGFR decline in IgA nephropathy

    Hayashi K., Hishikawa A., Hashiguchi A., Azegami T., Yoshimoto N., Nakamichi R., Tokuyama H., Itoh H.

    Scientific Reports (Scientific Reports)  10 ( 1 )  2020年12月

    研究論文(学術雑誌), 共著, 査読有り

     概要を見る

    © 2020, The Author(s). Accumulation of DNA double-strand breaks (DSBs) is linked to aging and age-related diseases. We recently reported the possible association of DNA DSBs with altered DNA methylation in murine models of kidney disease. However, DSBs and DNA methylation in human kidneys was not adequately investigated. This study was a cross-sectional observational study to evaluate the glomerular DNA DSB marker γH2AX and phosphorylated Ataxia Telangiectasia Mutated (pATM), and the DNA methylation marker 5-methyl cytosine (5mC) by immunostaining, and investigated the association with pathological features and clinical parameters in 29 patients with IgA nephropathy. To evaluate podocyte DSBs, quantitative long-distance PCR of the nephrin gene using laser-microdissected glomerular samples and immunofluorescent double-staining with WT1 and γH2AX were performed. Glomerular γH2AX level was associated with glomerular DNA methylation level in IgA nephropathy. Podocytopathic features were associated with increased number of WT1(+)γH2AX(+) cells and reduced amount of PCR product of the nephrin gene, which indicate podocyte DNA DSBs. Glomerular γH2AX and 5mC levels were significantly associated with the slope of eGFR decline over one year in IgA nephropathy patients using multiple regression analysis adjusted for age, baseline eGFR, amount of proteinuria at biopsy and immunosuppressive therapy after biopsy. Glomerular γH2AX level was associated with DNA methylation level, both of which may be a good predictor of renal outcome in IgA nephropathy.

  • Evaluation of Gleason Grade Group 5 in a Contemporary Prostate Cancer Grading System and Literature Review

    Kamitani R., Matsumoto K., Kosaka T., Takeda T., Hashiguchi A., Tanaka N., Morita S., Mizuno R., Shinojima T., Asanuma H., Oya M.

    Clinical Genitourinary Cancer (Clinical Genitourinary Cancer)  19 ( 1 ) 69 - 75.e5 2020年

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  15587673

     概要を見る

    © 2020 Elsevier Inc. Introduction: The aim of this study was to validate contemporary grading systems, in particular, the Gleason grade group (GGG) 5. Patients and Methods: We retrospectively reviewed the clinicopathologic data of 176 patients who underwent radical prostatectomy and whose pathologic results were GGG 4 or 5. The endpoints were biochemical recurrence (BCR) and castration-resistant prostate cancer (CRPC). Results: The GGG 4 group was composed of 69 patients. The GGG 5 group consisted of 78 patients with GS 4+5 and 29 patients with GS 5+4 or higher. The 5-year BCR-free survival rates for men with GGG 4, GS 4+5, and GS 5+4 or higher were 59%, 54%, and 20%, respectively, and the 5-year CRPC-free survival rates were 98%, 100%, and 88%, respectively. Both the BCR- and CRPC-free survival rates were significantly higher in GS 4+5 than in GS 5+4 or higher (P < .001 and P = .002, respectively), but there were no significant differences between GGG 4 and GS 4+5 (P = .702 and P = .803, respectively). The multivariate analysis demonstrated that GS 5+4 or higher (hazard ratio, 3.4; P = .002) and lymphovascular invasion (hazard ratio, 3.4; P < .001) greatly affected BCR. Conclusion: Our follow-up study revealed that men with GS 4+5 and those with GGG 4 had a similar prognosis. However, there was a significant discrepancy in prognosis between GS 4+5 and GS 5+4 or higher. This suggested that GGG 4 and 5 in the contemporary prostate cancer grading system should be reviewed. Furthermore, lymphovascular invasion may be useful to subgroup these pathologically high-risk patients.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

総説・解説等 【 表示 / 非表示

  • IgA腎症のOxford分類と日本分類(組織学的重症度分類)の腎機能予後予測に関する比較

    城 謙輔, 中里 毅, 橋口 明典, 清水 章, 久野 敏, 片渕 律子, 川村 哲也

    日本腎臓学会誌 ((一社)日本腎臓学会)  61 ( 3 ) 394 - 394 2019年05月

    その他記事, 共著,  ISSN  0385-2385

  • IgA腎症の生検時の臨床データと病理所見の関連性

    鎌野 千佐子, 清水 章, 城 謙輔, 橋口 明典, 久野 敏, 片渕 律子, 川村 哲也

    日本腎臓学会誌 ((一社)日本腎臓学会)  61 ( 3 ) 395 - 395 2019年05月

    その他記事, 共著,  ISSN  0385-2385

  • 前立腺癌の腫瘍局在が診断・治療・予後に及ぼす影響

    松本 一宏, 高松 公晴, 武田 利和, 小坂 威雄, 田中 伸之, 森田 伸也, 水野 隆一, 篠島 利明, 菊地 栄次, 浅沼 宏, 橋口 明典, 陣崎 雅弘, 大家 基嗣

    日本泌尿器科学会総会 ((一社)日本泌尿器科学会総会事務局)  107回   PP1 - 075 2019年04月

    その他記事, 共著

  • ステロイドパルス療法が有効であった腎移植後IgA腎症の1例

    森田 伸也, 高橋 遼平, 環 聡, 篠田 和伸, 香野 日高, 浅沼 宏, 橋口 明典, 脇野 修, 中川 健, 大家 基嗣

    日本臨床腎移植学会プログラム・抄録集 ((一社)日本臨床腎移植学会)  52回   264 - 264 2019年02月

    その他記事, 共著

  • C3腎症の視点から膜性増殖性糸球体腎炎(MPGN)の再検討 MPGN I型とIII型-2nd formは同一疾患か?

    佐藤 直実, 城 謙輔, 中村 保宏, 谷内 真司, 橋口 明典, 高木 孝士, 笹野 公伸

    日本病理学会会誌 ((一社)日本病理学会)  107 ( 1 ) 448 - 448 2018年04月

    その他記事, 共著,  ISSN  0300-9181

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研究発表 【 表示 / 非表示

  • Effectiveness of color correction on the quantitative analysis of histopathological images acquired by different whole-slide scanners

    Aziz Maulana Abdul, Nakamura Tomoya, Yamaguchi Masahiro, Kiyuna Tomoharu, Yamashita Yoshiko, Abe Tokiya, Hashiguchi Akinori, Sakamoto Michiie

    Artificial Life and Robotics, 2018年01月, その他

     概要を見る

    <p>Advances in whole-slide scanning have led to research on digital pathology, including monitor-based diagnosis, feature quantification, and computer-aided diagnosis. The color variations due to the staining process and scanning device are a serious issue that should be solved in whole-slide imaging applications, and methods for color correction have been studied. Nevertheless, the effectiveness of color-correction methods in the quantitative analysis of histopathological images acquired by different whole-slide scanners (WSSs) has not been confirmed. In this work, several liver tissue samples were scanned by different WSSs, and a color-correction method for whole-slide images was applied to the digitized tissue specimens. A set of histological features were extracted from the histopathological images, both without and with color correction, to analyze the effectiveness of the color-correction method in feature quantification and cancer identification.</p>

  • Extraction of glomeruli in whole slide imaging of kidney biopsy specimens

    Ishikawa Masahiro, Watanabe Sumire, Honda Natsuki, Kobayashi Naoki, Abe Tokiya, Hashiguchi Akinori, Sakamoto Michiie

    2017年

     概要を見る

    <p>The pathological diagnosis of a transplanted kidney is made on Banff Classification in order to gain an accurate understanding of the condition of the kidney. This type of diagnosis is extremely difficult and, thus, a variety of methods for diagnosis, including diagnosis by electron microscope, are being considered at present. Quantification of the diagnostic information derived by image processing is required for such purposes. This study proposes an automatic extraction method for normal glomeruli for the purpose of quantifying Elastica Van Gieson(EVG)-stained pathology specimens. In addition, we provide a report on the package of methods that we have created for the extraction of the glomerulus in the cortex.</p>

  • 木村病と抗好中球細胞質抗体(ANCA)関連腎炎を合併した再発性多発軟骨炎

    楢林敦,粟津緑,棗田とも,井ノ口美香子,新庄正宜,高橋孝雄,西川武二,佐藤慎二,平形道夫,亀山香織,橋口明典

    第501回日本小児科学会東京都地方会, 2003年03月, 口頭(一般)

  • In Vivo Model for Human Tumor Angiogenesis in Leukemia/Lymphoma or Multiple Myeloma and the Tumor Regression by Anti-Angiogenic Gene Therapy

    Yamada Taketo, Kondoh Kensuke, Hashiguchi Akinori, Hozumi Nobumichi, Suda Toshio, Sakamoto Michiie, Hata Jun-ichi

    Meeting of the Aerican Society of Hematology, 2002年12月, 口頭(一般)

  • Function of D4-GDI, a Rho GTPase Regulator, in T Cell Immunity

    Kondo Kensuke, Nakata Yuji, Kuwahara Mutsumi, Hashiguchi Akinori, Fujimoto Junichiroh, Hata Jun-ichi, Yamada Taketo

    Meeting of the Aerican Society of Hematology, 2002年12月, 口頭(一般)

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担当授業科目 【 表示 / 非表示

  • 病理学総論

    2021年度

  • 病理学各論

    2021年度

  • 病理学総論

    2020年度

  • 病理学各論

    2020年度

  • 病理学総論

    2019年度

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担当経験のある授業科目 【 表示 / 非表示

  • 臨床実習(病理診断部)

    慶應義塾, 2017年度, 専門科目, 実習・実験

  • 病理学各論(実習)

    慶應義塾, 2017年度, 専門科目, 実習・実験

  • 病理学総論(実習)

    慶應義塾, 2017年度, 専門科目, 実習・実験

  • 病理学各論(講義)

    慶應義塾, 2017年度, 専門科目, 講義

  • 臨床実習(病理診断部)

    慶應義塾, 2016年度, 専門科目, 実習・実験

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所属学協会 【 表示 / 非表示

  • 日本病理学会

     
  • 日本腎臓学会

     
  • 日本デジタルパソロジー研究会