橋口 明典 (ハシグチ アキノリ)

Hashiguchi, Akinori

写真a

所属(所属キャンパス)

医学部 電子顕微鏡研究室 (信濃町)

職名

専任講師

外部リンク

経歴 【 表示 / 非表示

  • 1995年04月
    -
    1997年03月

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学医学部助手(病理学)

  • 1997年04月
    -
    2007年03月

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学助手(医学部病理学)

  • 2007年04月
    -
    2013年11月

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学助教(医学部病理学)

  • 2013年12月
    -
    継続中

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学専任講師(医学部病理学)

学歴 【 表示 / 非表示

  • 1989年04月
    -
    1995年03月

    慶應義塾, 医学部

    日本, 大学, 卒業

学位 【 表示 / 非表示

  • 博士(医学), 慶應義塾, 論文, 2010年11月

    Using immunofluorescent digital slide technology to quantify protein expression in archival paraffin-embedded tissue sections

 

研究分野 【 表示 / 非表示

  • 人体病理学

研究キーワード 【 表示 / 非表示

  • デジタル病理学

  • 腎病理

 

論文 【 表示 / 非表示

  • Association of glomerular DNA damage and DNA methylation with one-year eGFR decline in IgA nephropathy

    Hayashi K., Hishikawa A., Hashiguchi A., Azegami T., Yoshimoto N., Nakamichi R., Tokuyama H., Itoh H.

    Scientific Reports (Scientific Reports)  10 ( 1 )  2020年12月

    研究論文(学術雑誌), 共著, 査読有り

     概要を見る

    © 2020, The Author(s). Accumulation of DNA double-strand breaks (DSBs) is linked to aging and age-related diseases. We recently reported the possible association of DNA DSBs with altered DNA methylation in murine models of kidney disease. However, DSBs and DNA methylation in human kidneys was not adequately investigated. This study was a cross-sectional observational study to evaluate the glomerular DNA DSB marker γH2AX and phosphorylated Ataxia Telangiectasia Mutated (pATM), and the DNA methylation marker 5-methyl cytosine (5mC) by immunostaining, and investigated the association with pathological features and clinical parameters in 29 patients with IgA nephropathy. To evaluate podocyte DSBs, quantitative long-distance PCR of the nephrin gene using laser-microdissected glomerular samples and immunofluorescent double-staining with WT1 and γH2AX were performed. Glomerular γH2AX level was associated with glomerular DNA methylation level in IgA nephropathy. Podocytopathic features were associated with increased number of WT1(+)γH2AX(+) cells and reduced amount of PCR product of the nephrin gene, which indicate podocyte DNA DSBs. Glomerular γH2AX and 5mC levels were significantly associated with the slope of eGFR decline over one year in IgA nephropathy patients using multiple regression analysis adjusted for age, baseline eGFR, amount of proteinuria at biopsy and immunosuppressive therapy after biopsy. Glomerular γH2AX level was associated with DNA methylation level, both of which may be a good predictor of renal outcome in IgA nephropathy.

  • Evaluation of Gleason Grade Group 5 in a Contemporary Prostate Cancer Grading System and Literature Review

    Kamitani R., Matsumoto K., Kosaka T., Takeda T., Hashiguchi A., Tanaka N., Morita S., Mizuno R., Shinojima T., Asanuma H., Oya M.

    Clinical Genitourinary Cancer (Clinical Genitourinary Cancer)  2020年

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  15587673

     概要を見る

    © 2020 Elsevier Inc. Introduction: The aim of this study was to validate contemporary grading systems, in particular, the Gleason grade group (GGG) 5. Patients and Methods: We retrospectively reviewed the clinicopathologic data of 176 patients who underwent radical prostatectomy and whose pathologic results were GGG 4 or 5. The endpoints were biochemical recurrence (BCR) and castration-resistant prostate cancer (CRPC). Results: The GGG 4 group was composed of 69 patients. The GGG 5 group consisted of 78 patients with GS 4+5 and 29 patients with GS 5+4 or higher. The 5-year BCR-free survival rates for men with GGG 4, GS 4+5, and GS 5+4 or higher were 59%, 54%, and 20%, respectively, and the 5-year CRPC-free survival rates were 98%, 100%, and 88%, respectively. Both the BCR- and CRPC-free survival rates were significantly higher in GS 4+5 than in GS 5+4 or higher (P < .001 and P = .002, respectively), but there were no significant differences between GGG 4 and GS 4+5 (P = .702 and P = .803, respectively). The multivariate analysis demonstrated that GS 5+4 or higher (hazard ratio, 3.4; P = .002) and lymphovascular invasion (hazard ratio, 3.4; P < .001) greatly affected BCR. Conclusion: Our follow-up study revealed that men with GS 4+5 and those with GGG 4 had a similar prognosis. However, there was a significant discrepancy in prognosis between GS 4+5 and GS 5+4 or higher. This suggested that GGG 4 and 5 in the contemporary prostate cancer grading system should be reviewed. Furthermore, lymphovascular invasion may be useful to subgroup these pathologically high-risk patients.

  • Maternal undernutrition aggravates renal tubular necrosis and interstitial fibrosis after unilateral ureteral obstruction in male rat offspring

    Awazu M., Abe T., Hashiguchi A., Hida M.

    PLoS ONE (PLoS ONE)  14 ( 9 )  2019年09月

    研究論文(学術雑誌), 共著, 査読有り

     概要を見る

    © 2019 Awazu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Maternal undernutrition is known to reduce glomerular number but it may also affect tubulointerstitium, capillary density, and response to oxidative stress. To investigate whether the latter elements are affected, we examined the response to unilateral ureteral obstruction (UUO), an established model of renal tubulointerstitial fibrosis, in the kidney of offspring from control and nutrient restricted rats. Six-week old male offspring from rats given food ad libitum (CON) and those subjected to 50% food restriction throughout pregnancy (NR) were subjected to UUO for 7 days. Body weight was significantly lower in NR. Systolic blood pressure and blood urea nitrogen increased similarly in CON and NR after UUO. Tubular necrosis in the obstructed kidney, on the other hand, was more extensive in NR. Also, the collagen area, a marker of fibrosis, of the obstructed kidney was significantly increased compared with the contralateral kidney only in NR. Capillary density was decreased similarly in the obstructed kidney of CON and NR compared with the contralateral kidney. Urine nitrate/ nitrite, a marker of nitric oxide production, from the obstructed kidney was significantly increased in NR compared with CON. Nitrotyrosine, a marker of nitric oxide-mediated free radical injury, was increased in the obstructed kidney compared with the contralateral kidney in both CON and NR, but the extent was significantly greater in NR. In conclusion, more severe tubular necrosis and fibrosis after UUO was observed in NR, which was thought to be due to increased nitrosative stress.

  • Acute Interstitial Nephritis With Karyomegalic Epithelial Cells After Nivolumab Treatment—Two Case Reports

    Ryuzaki M., Tokuyama H., Uchiyama K., Nakaya H., Hasegawa K., Miyashita K., Konishi K., Hashiguchi A., Wakino S., Itoh H.

    Clinical Medicine Insights: Case Reports (Clinical Medicine Insights: Case Reports)  12 2019年06月

    研究論文(学術雑誌), 共著, 査読有り

     概要を見る

    © The Author(s) 2019. Clinical application of immune checkpoint inhibitors (CPIs) including nivolumab is expanding in the field of oncology treatment. Nivolumab is an anti-programmed death 1 protein (PD-1) antibody designed to augment an immunologic reaction against cancer cells. On the contrary, CPIs are known to cause a unique variety of side effects termed as immune-related adverse events, which can affect any organ including kidney. However, the characteristics of renal disorders by nivolumab treatment are poorly described. We describe two cases of acute kidney injury that were treated with nivolumab. Two patients, one with renal-cell carcinoma and the other with lung cancer, exhibited progressive renal dysfunction after the initiation of nivolumab treatment. By kidney biopsy, each case was diagnosed as acute interstitial nephritis (AIN). Of note, tubular epithelial cells enlarged with hyperchromatic nuclei were focally observed, and this finding was consistent with karyomegalic tubular epithelial cells. In immunostaining, most of the enlarged tubular epithelial cells were positive for Ki-67, which suggested regeneration of tubular epithelial cells. Clinically, in one case, renal function was partially recovered with the discontinuation of nivolumab, while in another case renal function was fully recovered with additional corticosteroid treatment. We presented nivolumab-induced AIN with karyomegalic changes of tubular epithelia. We propose that immunosuppressive therapy may be necessary for the full recovery from renal impairment.

  • Effectiveness of color correction on the quantitative analysis of histopathological images acquired by different whole-slide scanners

    Aziz M., Nakamura T., Yamaguchi M., Kiyuna T., Yamashita Y., Abe T., Hashiguchi A., Sakamoto M.

    Artificial Life and Robotics (Artificial Life and Robotics)  24 ( 1 ) 28 - 37 2019年03月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  14335298

     概要を見る

    © 2018, ISAROB. Advances in whole-slide scanning have led to research on digital pathology, including monitor-based diagnosis, feature quantification, and computer-aided diagnosis. The color variations due to the staining process and scanning device are a serious issue that should be solved in whole-slide imaging applications, and methods for color correction have been studied. Nevertheless, the effectiveness of color-correction methods in the quantitative analysis of histopathological images acquired by different whole-slide scanners (WSSs) has not been confirmed. In this work, several liver tissue samples were scanned by different WSSs, and a color-correction method for whole-slide images was applied to the digitized tissue specimens. A set of histological features were extracted from the histopathological images, both without and with color correction, to analyze the effectiveness of the color-correction method in feature quantification and cancer identification.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

総説・解説等 【 表示 / 非表示

  • IgA腎症のOxford分類と日本分類(組織学的重症度分類)の腎機能予後予測に関する比較

    城 謙輔, 中里 毅, 橋口 明典, 清水 章, 久野 敏, 片渕 律子, 川村 哲也

    日本腎臓学会誌 ((一社)日本腎臓学会)  61 ( 3 ) 394 - 394 2019年05月

    その他記事, 共著,  ISSN  0385-2385

  • IgA腎症の生検時の臨床データと病理所見の関連性

    鎌野 千佐子, 清水 章, 城 謙輔, 橋口 明典, 久野 敏, 片渕 律子, 川村 哲也

    日本腎臓学会誌 ((一社)日本腎臓学会)  61 ( 3 ) 395 - 395 2019年05月

    その他記事, 共著,  ISSN  0385-2385

  • 前立腺癌の腫瘍局在が診断・治療・予後に及ぼす影響

    松本 一宏, 高松 公晴, 武田 利和, 小坂 威雄, 田中 伸之, 森田 伸也, 水野 隆一, 篠島 利明, 菊地 栄次, 浅沼 宏, 橋口 明典, 陣崎 雅弘, 大家 基嗣

    日本泌尿器科学会総会 ((一社)日本泌尿器科学会総会事務局)  107回   PP1 - 075 2019年04月

    その他記事, 共著

  • ステロイドパルス療法が有効であった腎移植後IgA腎症の1例

    森田 伸也, 高橋 遼平, 環 聡, 篠田 和伸, 香野 日高, 浅沼 宏, 橋口 明典, 脇野 修, 中川 健, 大家 基嗣

    日本臨床腎移植学会プログラム・抄録集 ((一社)日本臨床腎移植学会)  52回   264 - 264 2019年02月

    その他記事, 共著

  • C3腎症の視点から膜性増殖性糸球体腎炎(MPGN)の再検討 MPGN I型とIII型-2nd formは同一疾患か?

    佐藤 直実, 城 謙輔, 中村 保宏, 谷内 真司, 橋口 明典, 高木 孝士, 笹野 公伸

    日本病理学会会誌 ((一社)日本病理学会)  107 ( 1 ) 448 - 448 2018年04月

    その他記事, 共著,  ISSN  0300-9181

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研究発表 【 表示 / 非表示

  • Effectiveness of color correction on the quantitative analysis of histopathological images acquired by different whole-slide scanners

    Aziz Maulana Abdul, Nakamura Tomoya, Yamaguchi Masahiro, Kiyuna Tomoharu, Yamashita Yoshiko, Abe Tokiya, Hashiguchi Akinori, Sakamoto Michiie

    Artificial Life and Robotics, 2018年01月, その他

     概要を見る

    <p>Advances in whole-slide scanning have led to research on digital pathology, including monitor-based diagnosis, feature quantification, and computer-aided diagnosis. The color variations due to the staining process and scanning device are a serious issue that should be solved in whole-slide imaging applications, and methods for color correction have been studied. Nevertheless, the effectiveness of color-correction methods in the quantitative analysis of histopathological images acquired by different whole-slide scanners (WSSs) has not been confirmed. In this work, several liver tissue samples were scanned by different WSSs, and a color-correction method for whole-slide images was applied to the digitized tissue specimens. A set of histological features were extracted from the histopathological images, both without and with color correction, to analyze the effectiveness of the color-correction method in feature quantification and cancer identification.</p>

  • Extraction of glomeruli in whole slide imaging of kidney biopsy specimens

    Ishikawa Masahiro, Watanabe Sumire, Honda Natsuki, Kobayashi Naoki, Abe Tokiya, Hashiguchi Akinori, Sakamoto Michiie

    2017年

     概要を見る

    <p>The pathological diagnosis of a transplanted kidney is made on Banff Classification in order to gain an accurate understanding of the condition of the kidney. This type of diagnosis is extremely difficult and, thus, a variety of methods for diagnosis, including diagnosis by electron microscope, are being considered at present. Quantification of the diagnostic information derived by image processing is required for such purposes. This study proposes an automatic extraction method for normal glomeruli for the purpose of quantifying Elastica Van Gieson(EVG)-stained pathology specimens. In addition, we provide a report on the package of methods that we have created for the extraction of the glomerulus in the cortex.</p>

  • 木村病と抗好中球細胞質抗体(ANCA)関連腎炎を合併した再発性多発軟骨炎

    楢林敦,粟津緑,棗田とも,井ノ口美香子,新庄正宜,高橋孝雄,西川武二,佐藤慎二,平形道夫,亀山香織,橋口明典

    第501回日本小児科学会東京都地方会, 2003年03月, 口頭(一般)

  • Function of D4-GDI, a Rho GTPase Regulator, in T Cell Immunity

    Kondo Kensuke, Nakata Yuji, Kuwahara Mutsumi, Hashiguchi Akinori, Fujimoto Junichiroh, Hata Jun-ichi, Yamada Taketo

    Meeting of the Aerican Society of Hematology, 2002年12月, 口頭(一般)

  • In Vivo Model for Human Tumor Angiogenesis in Leukemia/Lymphoma or Multiple Myeloma and the Tumor Regression by Anti-Angiogenic Gene Therapy

    Yamada Taketo, Kondoh Kensuke, Hashiguchi Akinori, Hozumi Nobumichi, Suda Toshio, Sakamoto Michiie, Hata Jun-ichi

    Meeting of the Aerican Society of Hematology, 2002年12月, 口頭(一般)

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担当授業科目 【 表示 / 非表示

  • 病理学総論

    2021年度

  • 病理学各論

    2021年度

  • 病理学総論

    2020年度

  • 病理学各論

    2020年度

  • 病理学総論

    2019年度

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担当経験のある授業科目 【 表示 / 非表示

  • 臨床実習(病理診断部)

    慶應義塾, 2017年度, 専門科目, 実習・実験

  • 病理学各論(実習)

    慶應義塾, 2017年度, 専門科目, 実習・実験

  • 病理学総論(実習)

    慶應義塾, 2017年度, 専門科目, 実習・実験

  • 病理学各論(講義)

    慶應義塾, 2017年度, 専門科目, 講義

  • 臨床実習(病理診断部)

    慶應義塾, 2016年度, 専門科目, 実習・実験

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所属学協会 【 表示 / 非表示

  • 日本病理学会

     
  • 日本腎臓学会

     
  • 日本デジタルパソロジー研究会