Hashiguchi, Akinori

写真a

Affiliation

School of Medicine, Department of Pathology (Shinanomachi)

Position

Assistant Professor/Senior Assistant Professor

External Links

Career 【 Display / hide

  • 1995.04
    -
    1997.03

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学医学部助手(病理学)

  • 1997.04
    -
    2007.03

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学助手(医学部病理学)

  • 2007.04
    -
    2013.11

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学助教(医学部病理学)

  • 2013.12
    -
    Present

    慶應義塾大学医学部, 病理学教室, 慶應義塾大学専任講師(医学部病理学)

Academic Background 【 Display / hide

  • 1989.04
    -
    1995.03

    Keio University, School of Meicine

    Japan, University, Graduated

Academic Degrees 【 Display / hide

  • 博士(医学), Keio University, Dissertation, 2010.11

    Using immunofluorescent digital slide technology to quantify protein expression in archival paraffin-embedded tissue sections

 

Research Areas 【 Display / hide

  • Human pathology

Research Keywords 【 Display / hide

  • Digital Pathology

  • Renal Pathology

 

Papers 【 Display / hide

  • Effectiveness of color correction on the quantitative analysis of histopathological images acquired by different whole-slide scanners

    Aziz M., Nakamura T., Yamaguchi M., Kiyuna T., Yamashita Y., Abe T., Hashiguchi A., Sakamoto M.

    Artificial Life and Robotics (Artificial Life and Robotics)  24 ( 1 ) 28 - 37 2019.03

    ISSN  14335298

     View Summary

    © 2018, ISAROB. Advances in whole-slide scanning have led to research on digital pathology, including monitor-based diagnosis, feature quantification, and computer-aided diagnosis. The color variations due to the staining process and scanning device are a serious issue that should be solved in whole-slide imaging applications, and methods for color correction have been studied. Nevertheless, the effectiveness of color-correction methods in the quantitative analysis of histopathological images acquired by different whole-slide scanners (WSSs) has not been confirmed. In this work, several liver tissue samples were scanned by different WSSs, and a color-correction method for whole-slide images was applied to the digitized tissue specimens. A set of histological features were extracted from the histopathological images, both without and with color correction, to analyze the effectiveness of the color-correction method in feature quantification and cancer identification.

  • A grading system that predicts the risk of dialysis induction in IgA nephropathy patients based on the combination of the clinical and histological severity

    Okonogi H., Kawamura T., Joh K., Koike K., Miyazaki Y., Ogura M., Tsuboi N., Hirano K., Matsushima M., Yokoo T., Horikoshi S., Suzuki Y., Yasuda T., Shirai S., Shibata T., Hattori M., Akioka Y., Katafuchi R., Hashiguchi A., Hisano S., Shimizu A., Kimura K., Maruyama S., Matsuo S., Tomino Y.

    Clinical and Experimental Nephrology (Clinical and Experimental Nephrology)  23 ( 1 ) 16 - 25 2019.01

    ISSN  13421751

     View Summary

    © 2018, The Author(s). Histological classification is essential in the clinical management of immunoglobulin A nephropathy (IgAN). However, there are limitations in predicting the prognosis of IgAN based on histological information alone, which suggests the need for better prognostic models. Therefore, we defined a prognostic model by combining the grade of clinical severity with the histological grading system by the following processes. We included 270 patients and explored the clinical variables associated with progression to end-stage renal disease (ESRD). Then, we created a predictive clinical grading system and defined the risk grades for dialysis induction by a combination of the clinical grade (CG) and the histological grade (HG). A logistic regression analysis revealed that the 24-h urinary protein excretion (UPE) and the estimated glomerular filtration rate (eGFR) were significant independent variables. We selected UPE of 0.5 g/day and eGFR of 60 ml/min/1.73 m 2 as the threshold values for the classification of CG. The risk of progression to ESRD of patients with CG II and III was significantly higher than that of patients with CG I. The patients were then re-classified into nine compartments based on the combination of CG and HG. Furthermore, the nine compartments were grouped into four risk groups. The risk of ESRD in the moderate, high, and super-high-risk groups was significantly higher than that in the low-risk group. Herein, we are giving a detailed description of our grading system for IgA nephropathy that predicted the risk of dialysis based on the combination of CG and HG.

  • Non-invasive liver fibrosis assessment correlates with collagen and elastic fiber quantity in patients with hepatitis C virus infection

    Yasui Y., Abe T., Kurosaki M., Matsunaga K., Higuchi M., Tamaki N., Watakabe K., Okada M., Wang W., Shimizu T., Takaura K., Masugi Y., Nakanishi H., Tsuchiya K., Takahashi Y., Itakura J., Sakurai U., Hashiguchi A., Sakamoto M., Izumi N.

    Hepatology Research (Hepatology Research)  49 ( 1 ) 33 - 41 2019.01

    ISSN  13866346

     View Summary

    © 2018 The Japan Society of Hepatology Aim: Elastic fiber deposition is a cause of irreversibility of liver fibrosis. However, to date, its relevance to clinical features has not yet been clarified. This study aimed to clarify the correlation between non-invasive markers of fibrosis and fiber quantity, including elastic fiber, obtained from computational analysis. Methods: This retrospective study included 270 patients evaluated by non-invasive liver fibrosis assessment prior to liver biopsy. Of these patients, 95 underwent magnetic resonance elastography (MRE) and 244 were assessed with Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA + -M2BP). Using whole-slide imaging of Elastica van Gieson-stained liver biopsy sections, the quantity of collagen, elastin, and total fiber (elastin + collagen) was determined. Results: The total fiber quantity showed significant linear correlation with fibrosis stage F0–F4. Collagen fiber quantity increased from stage F0 to F4, whereas elastic fiber quantity increased significantly only from stage F2 to F3. Spearman's rank correlation test revealed that non-invasive liver fibrosis assessment significantly correlates with each fiber quantity, including correlation between total fiber quantity and the Fibrosis-4 (FIB-4) index (r = 0.361, P < 0.001), WFA + -M2BP values (r = 0.404, P < 0.001), and liver stiffness value by MRE (r = 0.615, P < 0.001). Receiver operating characteristic (ROC) curve analyses revealed that the area under ROC for predicting higher elastic fiber (>3.6%) is 0.731 by FIB-4 index, 0.716 by WFA + -M2BP, and 0.822 by liver stiffness by MRE. Conclusion: Liver fibrosis correlates with fiber quantity through non-invasive assessment regardless of fiber type, including elastic fiber. Moreover, MRE is useful for predicting high amounts of elastic fiber.

  • Prediction of extraprostatic extension by MRI tumor contact length: difference between anterior and posterior prostate cancer

    Matsumoto K., Akita H., Narita K., Hashiguchi A., Takamatsu K., Takeda T., Kosaka T., Mizuno R., Kikuchi E., Oya M., Jinzaki M.

    Prostate Cancer and Prostatic Diseases (Prostate Cancer and Prostatic Diseases)   2019

    ISSN  13657852

     View Summary

    © 2019, Springer Nature America, Inc. Background: Tumor contact length (TCL) is defined as the extent of contact between prostate cancer and the prostatic capsule, and its predictive value for microscopic extraprostatic extension (EPE) has been reported. However, the impact of the zonal origin (anterior or posterior tumor) of the tumor on the diagnosis of EPE is controversial. Methods: We retrospectively analyzed the records of 233 consecutive patients who underwent preoperative MRI and radical prostatectomy. We designated their tumors as anterior or posterior, and evaluated the correlation between the TCL measured by MRI and microscopic EPE in the radical prostatectomy specimen. Then, we created the predicted probability curves for EPE versus TCL for anterior and posterior prostate cancer. Results: There were 109 patients (47%) with an anterior tumor and 124 patients (53%) with a posterior tumor. Postoperative pathological analysis confirmed pT3 in 18 patients (17%) with an anterior tumor and in 53 patients (43%) with a posterior tumor. Multivariate analysis demonstrated that the zonal origin of the tumor was an independent predictive factor for EPE. We developed separate probability curves of EPE versus TCL for anterior and posterior prostate cancer, which revealed that anterior tumors were less likely to invade the extraprostatic tissues. Among patients whose TCL was 10–20 mm, 9/32 patients (28%) with an anterior tumor had EPE compared with 24/45 patients (53%) with a posterior tumor (p = 0.036). The decision curve of this EPE predictive model had high clinical efficacy. Conclusions: Our results indicate that anterior tumors have more favorable pathological characteristics than posterior tumors with the same TCL measured by MRI. We constructed two separate predicted probability curves for EPE after discriminating anterior and posterior tumors, which will be useful for decision making in clinical practice.

  • Mesenchymal-epithelial transition of pancreatic cancer cells at perineural invasion sites is induced by Schwann cells

    Fujii-Nishimura, Y., Yamazaki, K., Masugi, Y., Douguchi, J., Kurebayashi, Y., Kubota, N., Ojima, H., Kitago, M., Shinoda, M., Hashiguchi, A. and Sakamoto, M.

    Pathol Int (Pathology International)  68 ( 4 ) 214 - 223 2018.04

    ISSN  1440-1827

     View Summary

    Epithelial-mesenchymal transition (EMT) promotes invasion and metastasis of pancreatic ductal adenocarcinoma (PDAC). However, the importance of its reverse process, mesenchymal-epithelial transition (MET), for PDAC remains unclear. We aimed to characterize the histological finding "focal differentiation" in PDAC at perineural invasion sites in the context of MET and to investigate the role of Schwann cells in inducing tumor MET. Tumor differentiation and immunohistochemical expressions of E-cadherin, SMAD3, and vimentin at perineural invasion sites were examined in 168 PDAC tissues. Four PDAC cell lines were co-cultured with Schwann cells to investigate cell morphology, motility, or EMT-related markers using immunocytochemistry and quantitative PCR. Of 168 tumors, 124 (74%) showed focal differentiation with enhanced E-cadherin membrane expression (P < 0.001) and decreased nuclear accumulation of SMAD3 (P < 0.001). Among 115 PDACs harboring grade 1/2 tumor, tumors with focal differentiation showed worse survival compared to those without focal differentiation (P = 0.019). PDAC cells co-cultured with Schwann cells demonstrated a sheet-like appearance, increased E-cadherin expression, decreased expressions of SMAD3 and vimentin, and reduced cell motility. In conclusion, MET-like change is induced by Schwann cells, suggesting that Schwann cells contribute to PDAC colonization in pancreatic nerves through activating the MET machinery inside tumor cells in the pancreatic tumor microenvironment.

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Papers, etc., Registered in KOARA 【 Display / hide

Reviews, Commentaries, etc. 【 Display / hide

Presentations 【 Display / hide

  • Effectiveness of color correction on the quantitative analysis of histopathological images acquired by different whole-slide scanners

    Aziz Maulana Abdul, Nakamura Tomoya, Yamaguchi Masahiro, Kiyuna Tomoharu, Yamashita Yoshiko, Abe Tokiya, Hashiguchi Akinori, Sakamoto Michiie

    Artificial Life and Robotics, 2018.01

     View Summary

    <p>Advances in whole-slide scanning have led to research on digital pathology, including monitor-based diagnosis, feature quantification, and computer-aided diagnosis. The color variations due to the staining process and scanning device are a serious issue that should be solved in whole-slide imaging applications, and methods for color correction have been studied. Nevertheless, the effectiveness of color-correction methods in the quantitative analysis of histopathological images acquired by different whole-slide scanners (WSSs) has not been confirmed. In this work, several liver tissue samples were scanned by different WSSs, and a color-correction method for whole-slide images was applied to the digitized tissue specimens. A set of histological features were extracted from the histopathological images, both without and with color correction, to analyze the effectiveness of the color-correction method in feature quantification and cancer identification.</p>

  • Extraction of glomeruli in whole slide imaging of kidney biopsy specimens

    Ishikawa Masahiro, Watanabe Sumire, Honda Natsuki, Kobayashi Naoki, Abe Tokiya, Hashiguchi Akinori, Sakamoto Michiie

    2017

     View Summary

    <p>The pathological diagnosis of a transplanted kidney is made on Banff Classification in order to gain an accurate understanding of the condition of the kidney. This type of diagnosis is extremely difficult and, thus, a variety of methods for diagnosis, including diagnosis by electron microscope, are being considered at present. Quantification of the diagnostic information derived by image processing is required for such purposes. This study proposes an automatic extraction method for normal glomeruli for the purpose of quantifying Elastica Van Gieson(EVG)-stained pathology specimens. In addition, we provide a report on the package of methods that we have created for the extraction of the glomerulus in the cortex.</p>

  • 木村病と抗好中球細胞質抗体(ANCA)関連腎炎を合併した再発性多発軟骨炎

    Narabayashi Atsushi, Awazu Midori, Natsumeda Tomo, Inokuchi Mikako, Shinjou Masayoshi, Takahashi Takao, Nishikawa Takeji, Satou Shinji, Hiragata Michio, Kameyama Kaori, Hashiguchi Akinori

    第501回日本小児科学会東京都地方会, 2003.03, Oral Presentation(general)

  • In Vivo Model for Human Tumor Angiogenesis in Leukemia/Lymphoma or Multiple Myeloma and the Tumor Regression by Anti-Angiogenic Gene Therapy

    Yamada Taketo, Kondoh Kensuke, Hashiguchi Akinori, Hozumi Nobumichi, Suda Toshio, Sakamoto Michiie, Hata Jun-ichi

    Meeting of the Aerican Society of Hematology, 2002.12, Oral Presentation(general)

  • Function of D4-GDI, a Rho GTPase Regulator, in T Cell Immunity

    Kondo Kensuke, Nakata Yuji, Kuwahara Mutsumi, Hashiguchi Akinori, Fujimoto Junichiroh, Hata Jun-ichi, Yamada Taketo

    Meeting of the Aerican Society of Hematology, 2002.12, Oral Presentation(general)

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Courses Taught 【 Display / hide

  • GENERAL PATHOLOGY

    2020

  • DISEASES OF ORGAN SYSTEMS

    2020

  • GENERAL PATHOLOGY

    2019

  • DISEASES OF ORGAN SYSTEMS

    2019

Courses Previously Taught 【 Display / hide

  • 臨床実習(病理診断部)

    Keio University, 2017, Major subject, Laboratory work/practical work/exercise

  • 病理学各論(実習)

    Keio University, 2017, Major subject, Laboratory work/practical work/exercise

  • 病理学総論(実習)

    Keio University, 2017, Major subject, Laboratory work/practical work/exercise

  • 病理学各論(講義)

    Keio University, 2017, Major subject, Lecture

  • 臨床実習(病理診断部)

    Keio University, 2016, Major subject, Laboratory work/practical work/exercise

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Memberships in Academic Societies 【 Display / hide

  • The Japanese Society of Pathology

     
  • Japanese Society of Nephrology

     
  • The Japanese Society of Digital Pathology