論文 - 金井　弥栄

Human amniotic fluid stem cells have a unique potential to accelerate cutaneous wound healing with reduced fibrotic scarring like a fetus

Fukutake M., Ochiai D., Masuda H., Abe Y., Sato Y., Otani T., Sakai S., Aramaki-Hattori N., Shimoda M., Matsumoto T., Miyakoshi K., Kanai Y., Kishi K., Tanaka M.

Human Cell （Human Cell）  32 （ 1 ） 51 - 63 2019年01月

共著,  ISSN  09147470

　概要を見る

© 2018, Japan Human Cell Society and Springer Japan KK, part of Springer Nature. Adult wound healing can result in fibrotic scarring (FS) characterized by excess expression of myofibroblasts and increased type I/type III collagen expression. In contrast, fetal wound healing results in complete regeneration without FS, and the mechanism remains unclear. Amniotic fluid cells could contribute to scar-free wound healing, but the effects of human amniotic fluid cells are not well characterized. Here, we determined the effect of human amniotic fluid stem cells (hAFS) on FS during wound healing. Human amniotic fluid was obtained by amniocentesis at 15–17 weeks of gestation. CD117-positive cells were isolated and defined as hAFS. hAFS (1 × 10 6 ) suspended in PBS or cell-free PBS were injected around wounds created in the dorsal region of BALB/c mice. Wound size was macroscopically measured, and re-epithelialization in the epidermis, granulation tissue area in the dermis and collagen contents in the regenerated wound were histologically analyzed. The ability of hAFS to engraft in the wound was assessed by tracking hAFS labeled with PKH-26. hAFS fulfilled the minimal criteria for mesenchymal stem cells. hAFS injection into the wound accelerated wound closure via enhancement of re-epithelialization with less FS. The process was characterized by lower numbers of myofibroblasts and higher expression of type III collagen. Finally, transplanted hAFS were clearly observed in the dermis until day 7 implying that hAFS worked in a paracrine manner. hAFS can function in a paracrine manner to accelerate cutaneous wound healing, producing less FS, a process resembling fetal wound healing.

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Primary Peritoneal Low-grade Serous Carcinoma in a Patient with Lynch Syndrome: A Case Report

Makiuchi S., Yoshida H., Ishikawa M., Kojima N., Kanai Y., Kato T.

International Journal of Gynecological Pathology （International Journal of Gynecological Pathology）  39 （ 4 ） 327 - 332 2019年

共著,  ISSN  02771691

　概要を見る

Copyright © 2019 The Author(s). Lynch syndrome (LS) is an autosomal dominant disease caused by a germline mutation in DNA mismatch repair genes which increases the risk of several cancers such as endometrial and colorectal cancers. However, there are only a few reports of peritoneal malignancies in patients with LS. Herein, we report the first case of a primary peritoneal lowgrade serous carcinoma in a woman with LS and provide a literature review of peritoneal malignancies in patients with LS. The patient was a 72-yr-old gravid 2 para 2 Japanese woman with a germline mutation in MLH1. She had a history of colon cancer and endometrial cancer and was treated with total hysterectomy and bilateral salpingo-oophorectomy 14 yr ago. During the follow-up, peritoneal nodules were detected by abdominal computed tomography which were surgically resected. Pathologic examination revealed a low-grade serous carcinoma with cells positive for BerEP4, MOC31, CEA, and WT-1 and negative for BAP1, PAX8, MLH1, and PMS2, by immunohistochemistry. This case report and literature review show that peritoneal low-grade serous carcinoma can occur in patients with LS and that LS-related cancers usually precede primary peritoneal malignancies. The differential diagnosis for peritoneal nodules in patients with LS should, therefore, include peritoneal serous carcinoma and malignant mesothelioma besides metastasis of LS-related cancers. Considering the ambiguous immunophenotypes, a combination of immunohistologic markers would be useful for an accurate diagnosis of such cases.

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Prognosis method for renal cell cancer

Y Kanai, E Arai, Y Nemoto, T Yotani

US Patent App. 15/756,812 2019年

Prognosis method for renal cell cancer

Y Kanai, E Arai, Y Nemoto, T Yotani

US Patent App. 15/756,812 2019年

Prognosis method for renal cell cancer

Y Kanai, E Arai, Y Nemoto, T Yotani

US Patent App. 15/756,812 2019年

Prognosis method for renal cell cancer

Y Kanai, E Arai, Y Nemoto, T Yotani

US Patent App. 15/756,812 2019年

Physiological Functions of LMP2/β1i in the Female Reproductive System

T Hayashi, K Sano, T Ichimura, G Gur, H Aburatani, Y Kanai, D Zharhary, ...

2019年

Physiological Functions of LMP2/β1i in the Female Reproductive System

T Hayashi, K Sano, T Ichimura, G Gur, H Aburatani, Y Kanai, D Zharhary, ...

2019年

Assessment of homologous recombination deficiency phenotype in breast cancers in adolescents and young adults in the clinical setting

T Watanabe, T Honda, H Totsuka, M Yoshida, M Tanioka, K Shiraishi, ...

2019年

Assessment of homologous recombination deficiency phenotype in breast cancers in adolescents and young adults in the clinical setting

T Watanabe, T Honda, H Totsuka, M Yoshida, M Tanioka, K Shiraishi, ...

2019年

Assessment of homologous recombination deficiency phenotype in breast cancers in adolescents and young adults in the clinical setting

T Watanabe, T Honda, H Totsuka, M Yoshida, M Tanioka, K Shiraishi, ...

2019年

Assessment of homologous recombination deficiency phenotype in breast cancers in adolescents and young adults in the clinical setting

T Watanabe, T Honda, H Totsuka, M Yoshida, M Tanioka, K Shiraishi, ...

2019年

乳頭状腎細胞癌

新井 恵吏, 金井 弥栄

病理と臨床臨時増刊号「肉眼病理　症例から探る鑑別のヒント」 37   253 - 255 2019年

査読有り

• CiNii Research

集合管癌

新井 恵吏, 金井 弥栄

病理と臨床臨時増刊号「肉眼病理　症例から探る鑑別のヒント」 37   259 - 261 2019年

査読有り

• CiNii Research

精巣胚細胞腫瘍の転移

新井 恵吏, 金井 弥栄

病理と臨床臨時増刊号「肉眼病理　症例から探る鑑別のヒント」 37   299 - 301 2019年

査読有り

• CiNii Research

精巣胚細胞腫瘍

新井 恵吏, 金井 弥栄

病理と臨床臨時増刊号「肉眼病理　症例から探る鑑別のヒント」 37   294 - 296 2019年

査読有り

• CiNii Research

精巣悪性リンパ腫

新井 恵吏, 金井 弥栄

病理と臨床臨時増刊号「肉眼病理　症例から探る鑑別のヒント」 37   297 - 298 2019年

査読有り

• CiNii Research

嫌色素細胞がんとオンコサイトーマ

新井 恵吏, 金井 弥栄

病理と臨床臨時増刊号「肉眼病理　症例から探る鑑別のヒント」 37   256 - 258 2019年

査読有り

• CiNii Research

The functional analysis of miR-200 family in renal cell carcinoma

Gotoh Masahiro, Arai Eri, Yoshida Teruhiko, Kanai Yae

CANCER SCIENCE 109   446 - 446 2018年12月

共著,  ISSN  1349-7006

Accumulation of tumor associated macrophages by miR-27b regulated CSF1 in renal cell carcinoma

Matsumoto Daichi, Tanaka Keiyu, Arai Eri, Kanai Yae, Ohta Shigeki, Kawakami Yutaka

CANCER SCIENCE 109   1192 - 1192 2018年12月

共著,  ISSN  1349-7006