KEIO RESEARCHERS INFORMATION SYSTEM

Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 2002.03

  東京薬科大学, 生命科学部, 分子生命科学科

  University, Graduated

• 2004.03

  北里大学, 感染制御科学府

  Graduate School, Completed, Master's course

• 2008.03

  北里大学, 感染制御科学府

  Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• 感染制御科学博士, 北里大学大学院, Coursework, 2008.03

Research Areas 【 Display / hide 】

Research Areas 【 Display / hide 】

• Life Science / Bacteriology

Research Keywords 【 Display / hide 】

Research Keywords 【 Display / hide 】

• Mycoplasma pneumoniae

• Real-time PCR

• Streptococcus agalactiae

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Genetic characteristics and antibiotic resistance of Haemophilus influenzae isolates from pediatric patients with acute otitis media after introduction of 13-valent pneumococcal conjugate vaccine in Japan

  Ubukata K., Morozumi M., Sakuma M., Adachi Y., Mokuno E., Tajima T., Iwata S., Inagaki M., Kamakazu K., Atago Y., Kakurai S., Iwamoto J., Usui S., Ooyama S., Sugano S., Kuroda H., Kamide Y., Kamide M., Murakawa Y., Kaieda S., Kawaziri I., Kawai A., Kikuchi Y., Sawaki S., Kyou K., Kimura S., Kimura Y., Kimura Y., Sashiyou H., Yoneya H., Kosaka K., Komeda M., Kobayashi K., Sakaida Y., Sawada S., Satoh T., Satoh M., Shimizu T., Kimura O., Shimada J., Hori Y., Sugita R., Sugita Y., Uchizono A., Soeda K., Tabuchi T., Matsuoka C., Suzuki C., Chiba H., Tsuboi Y., Tomiyama M., Nakano K., Nakazawa T., Fukumoto A., Fukumoto A., Nagura M., Nagayama I., Satoh M., Nakamura K., Noguchi K., Shiiba K., Nogami K., Matsuda H., Shiba E., Hirabayashi K., Hiyoshi M., Yamagishi S., Ozu C., Maruyama J., Matsubara S., Suetake M., Miyashita M., Mogami A., Yamaguchi M., Yamaoka H., Yuta A., Yoshikawa T., Toyofuku M., Mori S., Inagaki M.

  Journal of Infection and Chemotherapy (Journal of Infection and Chemotherapy)   2019

  ISSN  1341321X

  　View Summary

  © 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases Acute otitis media (AOM) occurs commonly in pediatric populations. We examined resistance genotype, antibiotic susceptibility, quinolone (QL) resistance, and multilocus sequence type (MLST) among Haemophilus influenzae isolates causing AOM following introduction of pneumococcal conjugate vaccines in Japan. The AOM surveillance group included 69 participating otolaryngologists. Causative pathogens isolated from middle ear fluid (MEF) samples collected from 582 children with AOM were identified using both bacterial culture and real-time PCR. H. influenzae isolates among these pathogens were characterized by capsular type, resistance genotype, antibiotic susceptibility, QL resistance, and MLST. In 2016, H. influenzae was identified in 319 samples (54.8%), among which 72.4% (n = 231) tested positive by both culture and PCR; remaining H. influenzae cases were only PCR-positive. This proportion of H. influenzae positivity has increased significantly from 41.2% in 2006 (p < 0.001). Among culture-positive strains, genotypic β-lactamase-nonproducing ampicillin (AMP)-resistant (gBLNAR) strains were frequent (63.2%), with β-lactamase-nonproducing AMP-susceptible (gBLNAS) strains accounting for only 24.2%. Susceptibilities of gBLNAR to oral antimicrobials were best for tosufloxacin, followed by cefditoren and tebipenem; MIC 90 s were 0.031 μg/mL, 0.5 μg/mL, and 1 μg/mL, respectively. In 7 gBLNAR isolates (3.0%), QL susceptibility was low, owing to amino acid substitutions in GyrA and/or ParC. Sequence types identified numbered 107, including 28 that were new. Prevention of further increases in resistance to antimicrobial agents will require antibiotic selection based on characterization of causative pathogens in clinical practice.

  • Access to Document (DOI)

• Effects of pneumococcal conjugate vaccine on genotypic penicillin resistance and serotype changes, Japan, 2010–2017

  Ubukata K., Takata M., Morozumi M., Chiba N., Wajima T., Hanada S., Shouji M., Sakuma M., Iwata S.

  Emerging Infectious Diseases (Emerging Infectious Diseases)  24 ( 11 ) 2010 - 2020 2018.11

  ISSN  10806040

  　View Summary

  © 2018, Centers for Disease Control and Prevention (CDC). All rights reserved. To clarify year-to-year changes in capsular serotypes, resistance genotypes, and multilocus sequence types of Streptococcus pneumoniae, we compared isolates collected from patients with invasive pneumococcal disease before and after introductions of 7-and 13-valent pneumococcal conjugate vaccines (PCV7 and PVC13, respectively). From April 2010 through March 2017, we collected 2,856 isolates from children and adults throughout Japan. Proportions of PCV13 serotypes among children decreased from 89.0% in fiscal year 2010 to 12.1% in fiscal year 2016 and among adults from 74.1% to 36.2%. Although nonvaccine serotypes increased after introduction of PCV13, genotypic penicillin resistance decreased from 54.3% in 2010 to 11.2% in 2016 among children and from 32.4% to 15.5% among adults. However, genotypic penicillin resistance emerged in 9 nonvaccine serotypes, but not 15A and 35B. Multilocus sequence typing suggested that resistant strains among nonvaccine serotypes may have evolved from clonal complexes 156 and 81. A more broadly effective vaccine is needed.

  • Access to Document (DOI)

• Multilocus Sequence Typing of Mycoplasma pneumoniae, Japan, 2002-2016.

  Ando M, Morozumi M, Adachi Y, Ubukata K, Iwata S.

  Emerging Infectious Diseases journal 24   1895 - 1901 2018.10

  Research paper (scientific journal)

• Multilocus sequence typing of Mycoplasma pneumoniae, Japan, 2002–2016

  Ando M., Morozumi M., Adachi Y., Ubukata K., Iwata S.

  Emerging Infectious Diseases (Emerging Infectious Diseases)  24 ( 10 ) 1898 - 1901 2018.10

  ISSN  10806040

  　View Summary

  © 2018, Centers for Disease Control and Prevention (CDC). All rights reserved. In Japan, Mycoplasma pneumoniae resistance to mac-rolides is high. To compare sequence types (STs) of susceptible and resistant isolates, we performed multilocus sequence typing for 417 isolates obtained in Japan during 2002–2016. The most prevalent ST overall was ST3, for macrolide-resistant was ST19, and for macrolide-suscepti-ble were ST14 and ST7.

  • Access to Document (DOI)

• Etiology of Acute Otitis Media and Characterization of Pneumococcal Isolates After Introduction of 13-Valent Pneumococcal Conjugate Vaccine in Japanese Children.

  13-Valent Pneumococcal Conjugate Vaccine in Japanese Children. Ubukata K, Morozumi M, Sakuma M, Takata M, Mokuno E, Tajima T, Iwata S; and the AOM Surveillance Study Group.

  The Pediatric Infectious Disease Journal (The Pediatric infectious disease journal)  37 ( 6 ) 598 - 604 2018.06

  Research paper (scientific journal)

  　View Summary

  BACKGROUND: Acute otitis media is a leading cause of childhood morbidity and antibiotic prescriptions. We examined etiologic changes in acute otitis media after introduction of 13-valent pneumococcal conjugate vaccine as routine immunization for Japanese children in 2014. Serotypes, resistance genotypes, antibiotic susceptibilities and multilocus sequence typing of pneumococcal isolates were also characterized. METHODS: Otolaryngologists prospectively collected middle ear fluid from 582 children by tympanocentesis or sampling through a spontaneously ruptured tympanic membrane between June 2016 and January 2017. Causative pathogens were identified by bacterial culture and real-time polymerase chain reaction for bacteria. Serotypes, resistance genotypes, sequence types and susceptibilities to 14 antimicrobial agents were determined for pneumococcal isolates. RESULTS: At least 1 bacterial pathogen was identified in 473 of the samples (81.3%). Nontypeable Haemophilus influenzae (54.8%) was detected most frequently, followed by Streptococcus pneumoniae (25.4%), Streptococcus pyogenes (2.9%) and others. Pneumococci of current vaccine serotypes have decreased dramatically from 82.1% in 2006 to 18.5% (P < 0.001). Commonest serotypes were 15A (14.8%), 3 (13.9%) and 35B (11.1%). Serotype 3 was significantly less frequent among children receiving 13-valent pneumococcal conjugate vaccine compared with 7-valent pneumococcal conjugate vaccine (P = 0.002). Genotypic penicillin-resistant S. pneumoniae accounted for 28.7%, slightly less than in 2006 (34.2%; P = 0.393); the penicillin-resistant serotypes 15A and 35B had increased. Serotypes 15A, 3 and 35B most often belonged to sequence types 63, 180 and 558. CONCLUSIONS: Our findings are expected to assist in development of future vaccines, and they underscore the need for appropriate clinical choice of oral agents based on testing of causative pathogens.

  • Access to Document (DOI)

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Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Mechanism of combining antibacterial therapy led to enhancement of bactericidal activity and suppressing toxin production

  Morozumi, Miyuki

  科学研究費補助金研究成果報告書 2016

Presentations 【 Display / hide 】

Presentations 【 Display / hide 】

• Characteristics of Drug Resistance of Mycoplasma pneumoniae Isolates

  Morozumi Miyuki

  [International presentation]  Joint Congress of The 7th Meeting of the Asian Organization for Mycoplasmology (7th AOM), The 45th Meeting of the Japanese Society of Mycoplasmology (45th JSM) (Japan) , 

  2018.05

  , 

  Symposium, workshop panel (nominated)

• 少子・高齢化社会におけるGBS感染症の本質的変化(ベーシックレクチャー8）

  諸角 美由紀

  第91回日本感染症学会総会・学術講演会, 

  2017.04

  , 

  Symposium, workshop panel (nominated)

• 市中型呼吸器感染症に対する網羅的迅速診断法：その臨床への応用．

  諸角　美由紀

  第65回日本感染症学会東日本地方会学術集会, 

  2016.10

  , 

  Symposium, workshop panel (nominated)

• A群・G群溶血性レンサ球菌に対するβ-ラクタム系薬とアミノ配糖体薬の併用効果．

  諸角 美由紀，高田 美佐子，江原　佳史，安藤 万里子，足立　容子，生方　公子，岩田　敏．

  第65回日本感染症学会東日本地方会学術集会, 

  2016.10

  , 

  Oral presentation (general)

• 小児肺炎例から分離されたMycoplasma pneumoniaeの Multilocus Sequence Typing (MLST)解析

  諸角 美由紀，高田　美佐子，生方 公子，岩田 敏

  日本マイコプラズマ学会第43回学術集会, 

  2016.06

  , 

  Oral presentation (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• Mechanism of combining antibacterial therapy led to enhancement of bactericidal activity and suppressing toxin production

  2015.04

  -

  2017.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Principal investigator

  　View Summary

  Combinational chemotherapy of β-lactam and aminoglycoside antibiotics, which are having different action targets, synergistically increased bactericidal activity against β-hemolytic streptococci and Streptococcus pneumoniae isolated from patients with invasive infection. Morphologic changes after exposure to the agents were observed by transmission electron microscopy. Obvious abnormal image was observed after exposure to β-lactam and/or aminoglycoside antibiotics. In addition, GM concentration in the cells was measured using LC/MS/MS, resulting GM concentrations in the cells increased when it was combined with β-lactam antibiotics than the alone.
  These results suggest that combining β-lactam antibiotics with GM led to enhancement of bactericidal activity.

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• 森村豊明会奨励賞（北里大学）

  2010

• ASM Best oral presentation award

  2009

• 日本化学療法学会　学術奨励賞

  2007

• 上田記念感染症・化学療法研究奨励賞

  2005

• JIC Award 2004（年間最優秀論文賞）

  2005

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• TROPICAL MEDICINE AND PARASITOLOGY

  2024

• TROPICAL MEDICINE AND PARASITOLOGY

  2023

• TROPICAL MEDICINE AND PARASITOLOGY

  2022

• TROPICAL MEDICINE AND PARASITOLOGY

  2021

• TROPICAL MEDICINE AND PARASITOLOGY

  2020

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