Harada, Sei

写真a

Affiliation

School of Medicine, Department of Preventive Medicine and Public Health （Shinanomachi）

Position

Assistant Professor/Senior Assistant Professor
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Career 【 Display / hide 】

  • 2009.04
    -
    2011.03

    Kudanzaka Hospital, Physician

  • 2014.04
    -
    2019.03

    Keio University School of Medicine, Department of Preventive Medicine and Public Health, Assistant Professor

  • 2014.04
    -
    Present

    Keio University Institute of Advanced Biosciences, Assistant Professor

  • 2016.09
    -
    2017.08

    Department of Epidemiology and Biostatistics, Faculty of Medicine, Imperial College London, Visiting Researcher

  • 2019.04
    -
    Present

    Keio University School of Medicine, Department of Preventive Medicine and Public Health, Assistant Professor

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Academic Background 【 Display / hide 】

  • 2003.04
    -
    2009.03

    School of Medicine, Keio University

    University, Graduated

  • 2010.04
    -
    2016.02

    Keio University Graduate School of Medicine Doctoral programs

    Graduate School, Completed

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Academic Degrees 【 Display / hide 】

  • PhD (Medicine), Keio University, Coursework

    Metabolomic profiling reveals novel biomarkers of alcohol intake and alcohol-induced liver injury in community-dwelling men

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Licenses and Qualifications 【 Display / hide 】

  • Medical Doctor, 2009

  • Industrial Physician, 2011

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Research Areas 【 Display / hide 】

  • Life Science / Medical management and medical sociology

  • Life Science / Hygiene and public health (laboratory)

  • Life Science / Hygiene and public health (non-laboratory)

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Research Keywords 【 Display / hide 】

  • Cohort Study

  • Metabolomics

  • Molecular Epidemiology

  • Cardiovascular Disease Epidemiology

  • Epidemiology

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Research Themes 【 Display / hide 】

  • Large scale multi-omics epidemiological study using metabolomic analysis, 

    2012.04
    -
    Present

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Papers 【 Display / hide 】

  • Reliability of plasma polar metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry

    Harada, Sei, Hirayama, Akiyoshi, Chan, Queenie, Kurihara, Ayako, Fukai, Kota, Iida, Miho, Kato, Suzuka, Sugiyama, Daisuke, Kuwabara, Kazuyo, Takeuchi, Ayano, Akiyama, Miki, Okamura, Tomonori, Ebbels, Timothy M. D., Elliott, Paul, Tomita, Masaru, Sato, Asako, Suzuki, Chizuru, Sugimoto, Masahiro, Soga, Tomoyoshi, Takebayashi, Toru

    PLOS ONE （PLoS ONE)  13 （ 1 ）  2018.01

    Research paper (scientific journal), Joint Work,  ISSN  1932-6203

    　View Summary

    © 2018 Harada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Background Cohort studies with metabolomics data are becoming more widespread, however, large-scale studies involving 10,000s of participants are still limited, especially in Asian populations. Therefore, we started the Tsuruoka Metabolomics Cohort Study enrolling 11,002 community-dwelling adults in Japan, and using capillary electrophoresis-mass spectrometry (CE-MS) and liquid chromatography–mass spectrometry. The CE-MS method is highly amenable to absolute quantification of polar metabolites, however, its reliability for large-scale measurement is unclear. The aim of this study is to examine reproducibility and validity of large-scale CE-MS measurements. In addition, the study presents absolute concentrations of polar metabolites in human plasma, which can be used in future as reference ranges in a Japanese population. Methods Metabolomic profiling of 8,413 fasting plasma samples were completed using CE-MS, and 94 polar metabolites were structurally identified and quantified. Quality control (QC) samples were injected every ten samples and assessed throughout the analysis. Inter- and intra-batch coefficients of variation of QC and participant samples, and technical intraclass correlation coefficients were estimated. Passing-Bablok regression of plasma concentrations by CE-MS on serum concentrations by standard clinical chemistry assays was conducted for creatinine and uric acid. Results and conclusions In QC samples, coefficient of variation was less than 20% for 64 metabolites, and less than 30% for 80 metabolites out of the 94 metabolites. Inter-batch coefficient of variation was less than 20% for 81 metabolites. Estimated technical intraclass correlation coefficient was above 0.75 for 67 metabolites. The slope of Passing-Bablok regression was estimated as 0.97 (95% confidence interval: 0.95, 0.98) for creatinine and 0.95 (0.92, 0.96) for uric acid. Compared to published data from other large cohort measurement platforms, reproducibility of metabolites common to the platforms was similar to or better than in the other studies. These results show that our CE-MS platform is suitable for conducting large-scale epidemiological studies.

  • Gender-specific association of early age-related macular degeneration with systemic and genetic factors in a Japanese population

    Sasaki, Mariko, Harada, Sei, Kawasaki, Yumiko, Watanabe, Miki, Ito, Hidemi, Tanaka, Hideo, Takeuchi, Ayano, Tsubota, Kazuo, Takebayashi, Toru, Nishiwaki, Yuji, Kawasaki, Ryo

    SCIENTIFIC REPORTS （Scientific Reports)  8 （ 1 ）  2018.01

    Research paper (scientific journal), Accepted,  ISSN  2045-2322

    　View Summary

    © 2018 The Author(s). The Tsuruoka Metabolomics Cohort Study included subjects aged 35-74 years from participants in annual health check-up programs in Tsuruoka, Japan. The gender-specific associations of early age-related macular degeneration (AMD) with systemic and genetic factors was assessed cross-sectionally. Of these, 3,988 subjects had fundus photographs of sufficient quality, and early AMD was present in 12.3% and 10.3% of men and women, respectively. In men, higher levels of high-density lipoprotein cholesterol and lower levels of triglycerides were associated with increased odds of having early AMD after adjusting for potential risk factors (for each 1 mmol/L increase, odds ratio [OR]: 1.61 and 0.78, 95% confidence interval [CI]: 1.17-2.23 and 0.64-0.96, respectively). In women, higher levels of total cholesterol and low-density lipoprotein cholesterol were associated with increased risk of having early AMD (OR: 1.21 and 1.26, 95% CI: 1.01-1.44 and 1.03-1.53, respectively). Sub-analysis demonstrated that women with ARMS2 A69S polymorphisms had a stronger risk for early AMD (OR: 3.25, 95% CI: 2.10-5.04) than men (OR: 1.65, 95% CI: 1.02-2.69). Differential associations of early AMD with both systemic and genetic factors by sex were demonstrated in a Japanese cohort, which suggests that disease process of early AMD could be different by sex.

  • Metabolic Profiling of Total Physical Activity and Sedentary Behavior in Community-Dwelling Men

    Fukai, Kota, Harada, Sei, Iida, Miho, Kurihara, Ayako, Takeuchi, Ayano, Kuwabara, Kazuyo, Sugiyama, Daisuke, Okamura, Tomonori, Akiyama, Miki, Nishiwaki, Yuji, Oguma, Yuko, Suzuki, Asako, Suzuki, Chizuru, Hirayama, Akiyoshi, Sugimoto, Masahiro, Soga, Tomoyoshi, Tomita, Masaru, Takebayashi, Toru

    PLOS ONE 11 （ 10 ）  2016.10

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  1932-6203

  • Metabolomic profiling reveals novel biomarkers of alcohol intake and alcohol-induced liver injury in community-dwelling men (vol 21, pg 18, 2016)

    Harada, Sei, Takebayashi, Toru, Kurihara, Ayako, Akiyama, Miki, Suzuki, Asako, Hatakeyama, Yoko, Sugiyama, Daisuke, Kuwabara, Kazuyo, Takeuchi, Ayano, Okamura, Tomonori, Nishiwaki, Yuji, Tanaka, Taichiro, Hirayama, Akiyoshi, Sugimoto, Masahiro, Soga, Tomoyoshi, Tomita, Masaru

    ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE 21 （ 4 ） 283 - 284 2016.07

    Research paper (scientific journal), Joint Work,  ISSN  1342-078X

  • Profiling of plasma metabolites in postmenopausal women with metabolic syndrome

    Iida, Miho, Harada, Sei, Kurihara, Ayako, Fukai, Kota, Kuwabara, Kazuyo, Sugiyama, Daisuke, Takeuchi, Ayano, Okamura, Tomonori, Akiyama, Miki, Nishiwaki, Yuji, Suzuki, Asako, Hirayama, Akiyoshi, Sugimoto, Masahiro, Soga, Tomoyoshi, Tomita, Masaru, Banno, Kouji, Aoki, Daisuke, Takebayashi, Toru

    MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY 23 （ 7 ） 749 - 758 2016.07

    Research paper (scientific journal), Joint Work,  ISSN  1072-3714

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • 鶴岡メタボロームコホート研究

    Harada Sei

    実験医学 (羊土社)  35 ( 17 ) 74 - 78 2017.10

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • 新しいコホート研究の立ち上げと今後の展望—神戸研 究と鶴岡メタボロームコホート研究

    Harada Sei

    呼吸と循環 (医学書院)  64 ( 1 ) 71 - 77 2016

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • ヒトの網羅的解析とその統合解析による新型たばこの疾患リスクの早期評価とその展開

    2022.04
    -
    2026.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator

  • ゲノム・メタボローム・コホートによる肝障害発症経路の解明と個別的予防医療への応用

    2018.07
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

  • 腎機能低下を早期に予測するメタボロミクス・バイオマーカーの解明と予防医療への展開

    2018.04
    -
    2020.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists , Principal investigator

  • Genome-Metabolome Association Study for Hyperuricemia

    2015.04
    -
    2017.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Principal investigator

    　View Summary

    The genome-metabolome association study for hyperuricemia was conducted in the large scale metabolomics cohort study in Tsuruoka City, Japan. It was clarified that most of plasma metabolites were associated with the level of serum uric acid in this first study among Japanese. It was also shown that ABCG2 polymorphism was associated with the level of serum uric acid, as well as concentrations of plasma metabolites. The relation between hyperuricemia and plasma metabolomic profile was partly affected by ABCG2 polymorphism. In particular, the relation between hyperuricemia and N,N-dimethylglycine was different by ABCG2 polymorphism in both of male and female. These findings suggest that further researches may proceed the tailor-made preventive medicine using genome and metabolome for hyperuricemia.

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Awards 【 Display / hide 】

  • 85th The Japanese Society for Hygiene Young Researcher Award

    2016.03

  • 29th Japanese Heart Foundation Scholarship

    2016.02

  • 第29回日本心臓財団・バイエル薬品　海外留学助成

    2016

    Type of Award： Award from publisher, newspaper, foundation, etc.

  • 第85回日本衛生学会優秀演題賞

    2016

    Type of Award： Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • PUBLIC HEALTH 2 (ADVANCED)

    2024

  • PUBLIC HEALTH 1 (BASIC)

    2024

  • EPIDEMIOLOGY AND PREVENTIVE MEDICINE

    2024

  • PUBLIC HEALTH 2 (ADVANCED)

    2023

  • PUBLIC HEALTH 1 (BASIC)

    2023

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Courses Previously Taught 【 Display / hide 】

  • 衛生学

    慶應義塾大学医学部

    2018.04
    -
    2019.03

  • 公衆衛生学

    慶應義塾大学医学部

    2018.04
    -
    2019.03

  • 医療統計・医療情報学

    慶應義塾大学医学部

    2018.04
    -
    2019.03

  • Preventive Medicine and Environmental Health

    School of Medicine, Keio University

    2018.04
    -
    2019.03

  • Medical Statistics and Information

    School of Medicine, Keio University

    2018.04
    -
    2019.03

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