KEIO RESEARCHERS INFORMATION SYSTEM

Career 【 Display / hide 】

Career 【 Display / hide 】

• 2004.10

  -

  2007.03

  Columbia University / Howard Hughes Medical Institute, 博士研究員

• 2007.04

  -

  Present

  慶應義塾大学, 医学部薬理学教室, 専任講師

• 2012.04

  -

  Present

  横浜国立大学, 環境情報学府, 客員准教授

• 2016.12

  -

  Present

  Keio University, Department of Pharmacology, School of Medicine, Associate Professor

Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 　

  東京大学, 理学部, 生物化学科

  University, Graduated

• 　

  The Johns Hopkins University, School of Medicine, Department of Neuroscience

  United States, Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• Ph.D., The Johns Hopkins University, 2004

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Norepinephrine induces rapid and long-lasting phosphorylation and redistribution of connexin 43 in cortical astrocytes

  Nuriya, Mutsuo, Morita, Ayaka, Shinotsuka, Takanori, Yamada, Tomoko, Yasui, Masato

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (Biochemical and Biophysical Research Communications)  504 ( 4 ) 690 - 697 2018.10

  Research paper (scientific journal),  ISSN  0006-291X

  　View Summary

  © 2018 Elsevier Inc. Norepinephrine (NE) modulates brain functions depending on both the internal and external environment. While the neuromodulatory actions of NE have been well characterized, the response and involvement of cortical astrocytes to physiological noradrenergic systems remain largely unknown, especially at the molecular level. In this study, we biochemically characterize the action of NE on astrocytes of the murine neocortex. NE stimulation of acute brain slices rapidly increase phosphorylation of connexin 43 (Cx43) at Serine (Ser) 368, in slices from both juvenile and adolescent animals. The phosphorylation is mediated by the protein kinase C (PKC) pathway under the α1-adrenergic receptor and remains elevated for tens of minutes following brief exposure to NE, well after the intracellular calcium level returns to normal level, suggesting the plastic nature of this phosphorylation event. Importantly, this phosphorylation event persists in the absence of neuronal transmissions, suggesting that the effect of NE on Cx43 phosphorylation is induced directly on astrocytes. Furthermore, these NE-induced phosphorylations are associated with biochemical dissociation of Cx43 from gap-junctional plaques to non-junctional compartments. Finally, we show that pharmacological manipulation of the noradrenergic system using psychoactive drugs modulates phosphorylation of Cx43 in the cerebral cortex in vivo. These data suggest that NE acts directly on astrocytes in parallel with neurons and modulates functionally critical connexin channel proteins in a plastic manner. Thus, plasticity of astrocytes induced by the “gliomodulatory” actions of NE may play important roles in their physiological as well as pharmacological actions in the brain.

  • Access to Document (DOI)

• Direct posttranslational modification of astrocytic connexin 43 proteins by the general anesthetic propofol in the cerebral cortex

  Nuriya, Mutsuo, Yasui, Daisuke, Yamada, Tomoko, Aoki, Takuma, Yasui, Masato

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (Biochemical and Biophysical Research Communications)  497 ( 2 ) 734 - 741 2018.03

  Research paper (scientific journal),  ISSN  0006-291X

  　View Summary

  © 2018 Elsevier Inc. Propofol is widely used as a general anesthetic and is generally considered to exert its action by regulating neuronal firing via facilitation of GABAA receptors. However, accumulating evidence suggests that propofol also acts on astrocytes, including inhibitory effects on gap junctional coupling, but the underlying molecular mechanisms remain largely unknown. Here, using acute cortical brain slices prepared from mice, we characterize propofol-induced molecular changes in astrocytic gap junction protein connexin 43 (Cx43). Propofol does not change the protein expression level of Cx43 or its incorporation into gap junctional plaques, according to biochemical and immunohistochemical analyses. However, propofol alters migration pattern of Cx43 on western blot, suggesting changes in its posttranslational modifications. Indeed, this change is accompanied by an increase in the phosphorylation of Cx43 at serine 368, which is known to reduce permeability of Cx43 gap junctions. Finally, we show that this change occurs in the absence of neuronal firing or glutamatergic transmissions. Overall, these results show that propofol induces posttranslational modification of Cx43 directly on astrocytes at the site of gap junctional plaques, exerting direct pharmacological action on astrocytes in parallel with its action on neurons.

  • Access to Document (DOI)

• Background norepinephrine primes astrocytic calcium responses to subsequent norepinephrine stimuli in the cerebral cortex

  Nuriya, Mutsuo, Takeuchi, Miyabi, Yasui, Masato

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 483 ( 1 ) 732 - 738 2017.01

  Research paper (scientific journal),  ISSN  0006-291X

  • Access to Document (DOI)

• Multimodal two-photon imaging using a second harmonic generation-specific dye

  Nuriya, Mutsuo, Fukushima, Shun, Momotake, Atsuya, Shinotsuka, Takanori, Yasui, Masato, Arai, Tatsuo

  NATURE COMMUNICATIONS 7   732 - 738 2016.05

  Research paper (scientific journal),  ISSN  2041-1723

  • Access to Document (DOI)

• Astrovascular interaction

  Nuriya Mutsuo

  Folia Pharmacologica Japonica 145 ( 6 ) 326 - 328 2015.06

  ISSN  0015-5691

  • Access to Document (DOI)

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Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Characterization of structure function relatioship of astrocyte

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2018

• Visualization of biological phenomena by the second harmonic generation imaging using the next generation dye.

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2015

• Characterization of neuronal physiology by second harmonic generation imaging

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2013

• Regulation of dendritic voltage gated ion channels by neurotransmitters

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2010

• 医学研究における光技術の応用

  Nuriya, Mutsuo

  Journal of the Keio Medical Society (慶應医学会)  85 ( 1 ) 47 - 48 2008.04

  ISSN  03685179

Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• 化学情報伝達の直接的可視化解析

  2024.06

  -

  2026.03

  挑戦的研究（萌芽）, Principal investigator

• 新規可視化法の開発と応用による微視的薬物動態の解明

  2024.04

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  2027.03

  基盤研究(B), Principal investigator

• 組織内水のケミカルイメージングから迫る脳の生理学

  2020.07

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  2024.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Pioneering), Principal investigator

• アルキンタギングによる脳の病態生理学解明の新たなツールの開発と応用

  2020.04

  -

  2024.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

• アストロサイトの構造機能連関とその制御の解明

  2016.04

  -

  2019.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

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Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• PHARMACOLOGY

  2024

• PHARMACOLOGY

  2023

• PHARMACOLOGY

  2022

• PHARMACOLOGY

  2021

• PHARMACOLOGY

  2020

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Courses Previously Taught 【 Display / hide 】

Courses Previously Taught 【 Display / hide 】

• MCB-I

  Keio University

  2015.04

  -

  2016.03

• 基礎分子細胞学

  Keio University

  2015.04

  -

  2016.03

• 薬理学

  Keio University

  2015.04

  -

  2016.03