KEIO RESEARCHERS INFORMATION SYSTEM

Career 【 Display / hide 】

Career 【 Display / hide 】

• 2004.10

  -

  2007.03

  Columbia University / Howard Hughes Medical Institute, 博士研究員

• 2007.04

  -

  Present

  慶應義塾大学, 医学部薬理学教室, 専任講師

• 2012.04

  -

  Present

  横浜国立大学, 環境情報学府, 客員准教授

• 2016.12

  -

  Present

  Keio University, Department of Pharmacology, School of Medicine, Associate Professor

Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 　

  The Johns Hopkins University, School of Medicine, Department of Neuroscience

  United States, Graduate School, Completed, Doctoral course

• 　

  東京大学, 理学部, 生物化学科

  University, Graduated

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• Ph.D., The Johns Hopkins University, 2004

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Label-Free Visualization of Ciliary Rootlets in Mouse Brain

  Murakami Y., Nuriya M., Hu Z., Tomioka M., Oketani R., Hiramatsu K., Leproux P., Inoko A., Honjoh S., Kano H.

  Analytical Chemistry 97 ( 27 ) 14160 - 14167 2025.07

  ISSN  00032700

  　View Summary

  Neuronal primary cilia play important roles in brain development, sensory perception, and neurogenesis. Rootletin, a fibrous protein composed of coiled-coil motifs, is a key structural component of ciliary rootlets and is essential for understanding ciliary functions. However, the precise mechanisms by which Rootletin influences ciliary dynamics and impacts neuronal function remain largely unknown, primarily due to the challenges associated with visualizing these structures. In this study, we present a label-free, rapid, and highly sensitive method for visualizing Rootletin molecules in brain tissue. This platform integrates a second harmonic generation (SHG) microscope with background reduction by a one-step chemical pretreatment. Additionally, we employed coherent anti-Stokes Raman scattering imaging to simultaneously determine the cellular regions and intracellular locations of SHG signals. Applying this multimodal multiphoton imaging approach to the mouse hippocampus revealed that neuronal ciliary rootlets exhibited highly organized and specific intracellular distributions. Moreover, measurements of cultured neurons revealed that ciliary rootlets were detected even in immature neurons. These findings highlight the utility of our label-free imaging platform in developmental and neuroscience research, providing a powerful tool for characterizing ciliary dynamics and neuronal function.

  • Access to Document (DOI)

• Early neuromyelitis optica antibody-induced molecular changes in aquaporin 4 and associated proteins at astrocyte endfeet in murine brain tissues

  Yoshikawa Y., Tomioka M., Abe Y., Yasui M., Nuriya M.

  Journal of Pharmacological Sciences 158 ( 3 ) 212 - 218 2025.07

  ISSN  13478613

  　View Summary

  Neuromyelitis optica spectrum disorder (NMOSD) is characterized by the production of autoantibodies against aquaporin 4 (AQP4). Because NMOSD progressively causes irreversible and severe neurological damages, understanding the initial molecular changes induced by anti-AQP4 antibody binding is crucial for designing early interventions. However, knowledge about the effects of the antibodies before AQP4 loss in brain tissues is limited. Using acutely prepared mouse brain slices, we aimed to investigate the initial molecular impact of NMO model antibodies on AQP4 and its associated proteins. We employed two different types of NMO model antibodies, E5415A and E5415B; E5415A recognizes both M1 and M23 isoforms, whereas E5415B exclusively binds to M23. We found that E5415A but not E5415B disrupted the uniform perivascular localization of AQP4, leading to fragmentation. We further addressed the impact of these changes on AQP4-associated proteins and found that strong colocalizations between AQP4 and dystrophin-glycoprotein complex (DGC) components were preserved, even after AQP4 localization pattern became fragmented. Thus, our study reveals the initial molecular changes in the AQP4 channel at the astrocytic endfeet in response to NMO model antibodies and highlights the early pathological events occurring in NMOSD.

  • Access to Document (DOI)

• Spectral-focusing coherent anti-Stokes Raman scattering driven by bright correlated twin beams

  Eto Y., Nuriya M., Kano H.

  Physical Review Applied 23 ( 6 )  2025.06

  　View Summary

  An understanding of the interaction between bright correlated twin beams and matter is important for expanding the applications of optical quantum technology. In particular, it is worthwhile to clarify the advantages of twin-beam correlation in spectroscopic applications, since twin-beam sources that use parametric down-conversion have a wide range of wavelength tunability. Here, we theoretically and experimentally demonstrate that the spectral-focusing technique used in coherent anti-Stokes Raman scattering (CARS) can be implemented by twin beams without the use of ultrashort-pulse lasers. In typical spectral focusing using ultrashort pulses, spectral resolution is improved by narrowing the instantaneous frequency width due to frequency chirping. Similarly, we show theoretically that the formation of a chirp structure within the temporal correlation of twin beams produces an effect similar to typical spectral focusing. To experimentally demonstrate twin-beam spectral focusing, CARS spectroscopy of polyethylene is performed when the time delay between the chirped twin beams is set within the correlation width or it is set outside the correlation width. We observe that the spectral resolution is improved when the correlation is effective. By comparing with theoretical simulations, we confirm that the improvement is caused by frequency chirping.

  • Access to Document (DOI)

• Molecular identification of second harmonic generation (SHG) sources in mouse brain by multimodal imaging with ultra-broadband multiplex coherent anti Stokes Raman scattering (CARS)

  Murakami Y., Oketani R., Leproux P., Nuriya M., Honjoh S., Hideaki K.

  Progress in Biomedical Optics and Imaging Proceedings of SPIE 12855 2024

  ISSN  16057422

  　View Summary

  Second harmonic generation imaging is a powerful tool for visualizing molecular structures in living organisms without the need for exogenous dyes. However, SHG signal lacks molecular specificity in identifying the source. This study aimed at molecular identification of SHG sources in the mouse brain using a multimodal imaging technique combining SHG and multiplex coherent anti Stokes Raman scattering (CARS) spectroscopy. We performed multimodal imaging in two different regions, the surface and dentate gyrus of the brain tissue. For the brain surface, the SHG signal was recognized through CARS spectrum analysis, indicating its origin in collagen. In the dentate gyrus, CARS images did not unveil corresponding molecular origins; however, morphologically, the SHG signal likely originated from Rootletin within neurons. Overall, Multimodal imaging approach to molecular identification of SHG has the potential to contribute to a comprehensive understanding of the molecular and structural features of the mouse brain. These findings advance label-free imaging techniques and have implications for brain tissue analysis and functional mapping research.

  • Access to Document (DOI)

• Stimulated Raman scattering microscopy reveals a unique and steady nature of brain water dynamics

  Shinotsuka T., Miyazawa T., Karasawa K., Ozeki Y., Yasui M., Nuriya M.

  Cell Reports Methods 3 ( 7 )  2023.07

  　View Summary

  The biological activities of substances in the brain are shaped by their spatiotemporal dynamics in brain tissues, all of which are regulated by water dynamics. In contrast to solute dynamics, water dynamics have been poorly characterized, owing to the lack of appropriate analytical tools. To overcome this limitation, we apply stimulated Raman scattering multimodal multiphoton microscopy to live brain tissues. The microscopy system allows for the visualization of deuterated water, fluorescence-labeled solutes, and cellular structures at high spatiotemporal resolution, revealing that water moves faster than fluorescent molecules in brain tissues. Detailed analyses demonstrate that water, unlike solutes, diffuses homogeneously in brain tissues without differences between the intra- and the extracellular routes. Furthermore, we find that the water dynamics are steady during development and ischemia, when diffusions of solutes are severely affected. Thus, our approach reveals routes and uniquely robust properties of water diffusion in brain tissues.

  • Access to Document (DOI)

display all >>

Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Development and application of novel tools for pathophysiological investigation of the brain by an alkyne-tagging approach

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2023

• Physiology of the brain approached by chemical imaging of water inside tissues

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2023

• Characterization of structure function relatioship of astrocyte

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2018

• Visualization of biological phenomena by the second harmonic generation imaging using the next generation dye.

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2015

• Characterization of neuronal physiology by second harmonic generation imaging

  Nuriya, Mutsuo

  科学研究費補助金研究成果報告書 2013

display all >>

Reviews, Commentaries, etc. 【 Display / hide 】

Reviews, Commentaries, etc. 【 Display / hide 】

• Uses of coherent Raman scattering microscopy in neuroscience

  Nuriya M.

  Frontiers in Neuroscience 19 2026

  ISSN  16624548

  　View Summary

  Multiphoton microscopy allows the imaging of biological phenomena deep within brain tissues and has greatly advanced knowledge in neuroscience. However, many optical phenomena other than the multiphoton excitation of fluorophores in nonlinear optics are underrecognized. Coherent Raman scattering (CRS) uses multiple photons to boost weak Raman scattering. CRS has been used to enable molecular vibration-dependent contrast imaging of tissues and has been particularly useful for pathophysiological investigations of brain tissues. Recently, the combination of CRS with Raman-active bio-orthogonal chemical bonds or groups has proven particularly powerful for visualizing molecules not detectable by fluorescence imaging. This review introduces a new and exciting imaging strategy and its applications in neuroscience.

  • Access to Document (DOI)

Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• 化学情報伝達の直接的可視化解析

  2024.06

  -

  2026.03

  挑戦的研究（萌芽）, Principal investigator

• 新規可視化法の開発と応用による微視的薬物動態の解明

  2024.04

  -

  2027.03

  基盤研究(B), Principal investigator

• 組織内水のケミカルイメージングから迫る脳の生理学

  2020.07

  -

  2024.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Pioneering), Principal investigator

• アルキンタギングによる脳の病態生理学解明の新たなツールの開発と応用

  2020.04

  -

  2024.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

• アストロサイトの構造機能連関とその制御の解明

  2016.04

  -

  2019.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

display all >>

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• PHARMACOLOGY

  2025

• PHARMACOLOGY

  2024

• PHARMACOLOGY

  2023

• PHARMACOLOGY

  2022

• PHARMACOLOGY

  2021

display all >>

Courses Previously Taught 【 Display / hide 】

Courses Previously Taught 【 Display / hide 】

• MCB-I

  Keio University

  2015.04

  -

  2016.03

• 基礎分子細胞学

  Keio University

  2015.04

  -

  2016.03

• 薬理学

  Keio University

  2015.04

  -

  2016.03