Monkawa, Toshiaki

写真a

Affiliation

School of Medicine, Medical Education Center (Shinanomachi)

Position

Professor

External Links

Other Affiliation 【 Display / hide

  • Media Center, 信濃町メディアセンター所長

Career 【 Display / hide

  • 1991.04
    -
    1992.03

    慶應義塾大学病院内科研修医

  • 1996.01
    -
    1998.12

    学術振興会特別研究員(PD)

  • 1999.01
    -
    1996.06

    慶應義塾大学医学部助手(医学部)

  • 1999.07
    -
    2002.03

    Division of Nephrology, University of Washington Research Fellow

  • 2002.04
    -
    2007.03

    慶應義塾大学医学部腎臓内分泌代謝内科助手

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Academic Background 【 Display / hide

  • 1991

    Keio University, School of Medicine

    University, Graduated

  • 1996.03

    Keio University, Graduate School, Division of Medicine, 内科学

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • 博士(医学), Keio University, Coursework, 1996.03

Licenses and Qualifications 【 Display / hide

  • 医師免許, 1991.05

  • 日本内科学会認定内科医, 1996.09

  • 労働衛生コンサルタント(保健衛生), 1998.06

  • 日本腎臓学会腎臓専門医, 2003.04

  • 日本透析医学会専門医, 2005.04

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Research Areas 【 Display / hide

  • Kidney internal medicine (Nephrology)

  • 医学教育学

Research Keywords 【 Display / hide

  • 医学教育学

  • electrolyte, acid-base disorder

Research Themes 【 Display / hide

  • Interprofessional education, 

    2011.04
    -
    Present

  • 医学教育学, 

    2011.04
    -
    Present

  • electrolyte, acid-base disorder, 

    2002.04
    -
    Present

  • development and induction of renal tubular cells, 

    2002.04
    -
    Present

 

Books 【 Display / hide

  • ハルペリン 病態から考える電解質異常

    Kamel S. Kamel、Mitchell L. Halperin著、門川俊明 翻訳, メディカルサイエンスインターナショナル, 2018.06

  • なぜパターン認識だけで腎病理は読めないのか?

    MONKAWA TOSHIAKI, 医学書院, 2017.05

  • 電解質輸液塾

    MONKAWA TOSHIAKI, 中外医学社, 2013.04

  • レジデントのための血液透析患者マネジメント

    MONKAWA TOSHIAKI, 医学書院, 2011.06

  • 研究留学術

    MONKAWA TOSHIAKI, 医歯薬出版, 2002.07

Papers 【 Display / hide

  • Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors

    Hiratsuka K., Monkawa T., Akiyama T., Nakatake Y., Oda M., Goparaju S., Kimura H., Chikazawa-Nohtomi N., Sato S., Ishiguro K., Yamaguchi S., Suzuki S., Morizane R., Ko S., Itoh H., Ko M.

    Scientific Reports (Scientific Reports)  9 ( 1 )  2019.12

     View Summary

    © 2019, The Author(s). The derivation of kidney tissues from human pluripotent stem cells (hPSCs) and its application for replacement therapy in end-stage renal disease have been widely discussed. Here we report that consecutive transfections of two sets of synthetic mRNAs encoding transcription factors can induce rapid and efficient differentiation of hPSCs into kidney tissues, termed induced nephron-like organoids (iNephLOs). The first set - FIGLA, PITX2, ASCL1 and TFAP2C, differentiated hPSCs into SIX2+SALL1+ nephron progenitor cells with 92% efficiency within 2 days. Subsequently, the second set - HNF1A, GATA3, GATA1 and EMX2, differentiated these cells into PAX8+LHX1+ pretubular aggregates in another 2 days. Further culture in both 2-dimensional and 3-dimensional conditions produced iNephLOs containing cells characterized as podocytes, proximal tubules, and distal tubules in an additional 10 days. Global gene expression profiles showed similarities between iNephLOs and the human adult kidney, suggesting possible uses of iNephLOs as in vitro models for kidneys.

  • Generation of kidney tubular organoids from human pluripotent stem cells

    Yamaguchi Shintaro, Morizane Ryuji, Homma Koichiro, Monkawa Toshiaki, Suzuki Sayuri, Fujii Shizuka, Koda Muneaki, Hiratsuka Ken, Yamashita Maho, Yoshida Tadashi, Wakino Shu, Hayashi Koichi, Sasaki Junichi, Hori Shingo, Itoh Hiroshi

    Scientific Reports 6 2016.12

    ISSN  2045-2322

     View Summary

    <p>Recent advances in stem cell research have resulted in methods to generate kidney organoids from human pluripotent stem cells (hPSCs), which contain cells of multiple lineages including nephron epithelial cells. Methods to purify specific types of cells from differentiated hPSCs, however, have not been established well. For bioengineering, cell transplantation, and disease modeling, it would be useful to establish those methods to obtain pure populations of specific types of kidney cells. Here, we report a simple two-step differentiation protocol to generate kidney tubular organoids from hPSCs with direct purification of KSP (kidney specific protein)-positive cells using anti-KSP antibody. We first differentiated hPSCs into mesoderm cells using a glycogen synthase kinase-3β inhibitor for 3 days, then cultured cells in renal epithelial growth medium to induce KSP+ cells. We purified KSP+ cells using flow cytometry with anti-KSP antibody, which exhibited characteristics of all segments of kidney tubular cells and cultured KSP+ cells in 3D Matrigel, which formed tubular organoids in vitro. The formation of tubular organoids by KSP+ cells induced the acquisition of functional kidney tubules. KSP+ cells also allowed for the generation of chimeric kidney cultures in which human cells self-assembled into 3D tubular structures in combination with mouse embryonic kidney cells.</p>

  • Immunoadsorption therapy for dilated cardiomyopathy using tryptophan column-A prospective, multicenter, randomized, within-patient and parallel-group comparative study to evaluate efficacy and safety

    Yoshikawa Tsutomu, Baba Akiyasu, Akaishi Makoto, Wakabayashi Yasuhisa, Monkawa Toshiaki, Kitakaze Masafumi, Izumi Tohru, Tomoike Hitonobu

    Journal of Clinical Apheresis  2016

    ISSN  0733-2459

     View Summary

    <p>Over the past few decades, several cardiac autoantibodies have been reported in sera from patients with dilated cardiomyopathy (DCM). Immunoadsorption (IA) therapy is one of the therapeutic tools to remove such autoantibodies. The objective of this study was to investigate functional effects of IA therapy using a tryptophan column in severe DCM patients. Of 49 patients enrolled, 44 were randomized from 10 sites in Japan. IA therapy was conducted in 40 patients with DCM (refractory to standard therapy for heart failure, New York Heart Association [NYHA] class III/IV, left ventricular ejection fraction [LVEF] </p>

  • Fluid and electrolytes

    Monkawa Toshiaki

    Japanese Journal of Nephrology 58 ( 1 ) 12 - 16 2016

    ISSN  0385-2385

  • A case of severe osteomalacia caused by Tubulointerstitial nephritis with Fanconi syndrome in asymptomotic primary biliary cirrhosis

    Yamaguchi Shintaro, Maruyama Tatsuya, Wakino Shu, Tokuyama Hirobumi, Hashiguchi Akinori, Tada Shinichiro, Homma Koichiro, Monkawa Toshiaki, Thomas James, Miyashita Kazutoshi, Kurihara Isao, Yoshida Tadashi, Konishi Konosuke, Hayashi Koichi, Hayashi Matsuhiko, Itoh Hiroshi

    BMC Nephrology  2015.11

     View Summary

    <p>Background: Primary biliary cirrhosis (PBC) is an immune-mediated chronic cholestatic liver disease, characterized by increased concentrations of serum IgM and the presence of circulating anti-mitochondrial antibodies. Although bone diseases such as osteoporosis or osteodystrophy are commonly associated with PBC, osteomalacia which is caused by abnormal vitamin D metabolism, mineralization defects, and phosphate deficiency has not been recognized as a complication of PBC. Case presentation: We report the case of a 49-year-old Japanese woman who complained of multiple fractures. Hypophosphatemic osteomalacia was diagnosed from a low serum phosphorus level, 1,25-dihydroxyvitamin D3 level, high levels of bone specific alkaline phosphatase and the findings of bone scintigraphy, although a bone biopsy was not performed. Twenty four hour urine demonstrated a low renal fractional tubular reabsorption of phosphate, increased fractional excretion of uric acid and generalized aminoaciduria. An intravenous bicarbonate loading test suggested the presence of proximal renal tubular acidosis (RTA). These biochemical data indicated Fanconi syndrome with proximal RTA. A kidney biopsy demonstrated the features of tubulointerstitial nephritis (TIN). Conclusion: In this case, asymptomatic PBC was shown to induce TIN with Fanconi syndrome with dysregulation of electrolytes and vitamin D metabolism, which in turn led to osteomalacia with multiple fractures. Osteomalacia has not been recognized as a result of the renal involvement of PBC. PBC and its rare complication of TIN with Fanconi syndrome should be considered in adult patients with unexplained osteomalacia even in the absence of liver dysfunction.</p>

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Papers, etc., Registered in KOARA 【 Display / hide

Presentations 【 Display / hide

  • 慶應義塾大学における海外施設での臨床実習プログラム

    MONKAWA TOSHIAKI

    第49回日本教育学会大会, 2017.08, Oral Presentation(general)

  • もう一度やり直す腎生理

    MONKAWA TOSHIAKI

    第46回日本腎臓学会東部学術大会, 2016.10, Symposium, Workshop, Panelist (public offering)

  • タブレット端末iPad配付と教学システムのデジタル化

    MONKAWA TOSHIAKI

    第48回日本教育学会大会, 2016.07, Oral Presentation(general)

  • 腎臓専門医制度 腎臓専門医(内科)のカリキュラム

    MONKAWA TOSHIAKI

    第59回日本腎臓学会学術大会, 2016.06, Symposium, Workshop, Panelist (public offering)

  • K代謝異常

    MONKAWA TOSHIAKI

    第25回臨床内分泌代謝アップデート, 2015.11, Symposium, Workshop, Panelist (nomination)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Master regulatory factors for regeneration and EMT of kidney tubular cells

    2015.04
    -
    2018.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 門川 俊明, Grant-in-Aid for Scientific Research (C), Principal Investigator

Awards 【 Display / hide

  • Best Teacher

    2015.03, Keio University School of Medicine

  • 日本内科学会奨励賞

    MONKAWA TOSHIAKI, 2004.04, 日本内科学会

    Type of Award: Awards of National Conference, Council and Symposium

 

Courses Taught 【 Display / hide

  • BIOCHEMISTRY AND PHYSIOLOGY FOR NURSING AND HEALTH CARE

    2019

  • CLINICAL CLERKSHIP (ELECTIVE)

    2019

  • CLINICAL REASONING

    2019

  • CLINICAL TRAINING IN DIAGNOSIS

    2019

  • COMMUNITY-BASED CLINICAL CLERKSHIP (ELECTIVE)

    2019

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Courses Previously Taught 【 Display / hide

  • 地域基盤型臨床実習

    Keio University, 2017, Full academic year, Major subject, Laboratory work/practical work/exercise, Within own faculty, 110people

  • 選択臨床実習

    Keio University, 2017, Full academic year, Major subject, Laboratory work/practical work/exercise

  • 診断学実習

    Keio University, 2017, Full academic year, Major subject, Laboratory work/practical work/exercise, Within own faculty

  • CLINICAL REASONING

    Keio University, 2015, Full academic year, Major subject, Laboratory work/practical work/exercise, Within own faculty, 118people

  • CLINICAL TRAINING IN DIAGNOSIS

    Keio University, 2015, Full academic year, Major subject, Laboratory work/practical work/exercise, Within own faculty, 118people

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Educational Activities and Special Notes 【 Display / hide

  • なぜパターン認識だけで腎病理は読めないのか?

    2017.05

    , Development of Textbook and Teaching Material

  • Denkaishitsu-Yueki-Juku

    2013.04

    , Development of Textbook and Teaching Material

  • Management of hemodialysis patient for residents

    2011.06

    , Development of Textbook and Teaching Material

  • Interprofessional education at Keio University

    2011.04
    -
    Present

    , Special Affairs

 

Social Activities 【 Display / hide

  • 医学中央雑誌

    2010
    -
    Present
  • 医学のあゆみ

    2007
    -
    Present

Memberships in Academic Societies 【 Display / hide

  • 日本腎臓学会, 

    1995.11
    -
    Present
  • 日本透析医学会

     
  • 日本内科学会

     
  • 日本医学教育学会, 

    2008
    -
    Present

Committee Experiences 【 Display / hide

  • 1995.11
    -
    Present

    評議員, 日本腎臓学会

  •  

    会員, 日本透析医学会

  •  

    Member, 日本内科学会

  • 2008
    -
    Present

    representative, 日本医学教育学会

  • 2010
    -
    Present

    Editor, 医学中央雑誌

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