Furukawa, Ryohei



Faculty of Letters (Hiyoshi)


Associate Professor

Related Websites

Contact Address

神奈川県横浜市港北区日吉4−1−1 第2校舎2309

Telephone No.


Other Affiliation 【 Display / hide

  • Research and Education Center for Natural Sciences

Academic Background 【 Display / hide

  • 2010.04

    Keio University, 理工学研究科, 基礎理工学

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • 博士(理学), Keio University, Coursework, 2012.09



Research Areas 【 Display / hide

  • Immunobiology

  • Life Science / Developmental biology (Developmental Biology)

  • Life Science / Cell biology (Cell Biology)

  • Life Science / Molecular biology (Molecular Biology)

  • Life Science / Evolutionary biology (Evolutionary Biology)

Research Keywords 【 Display / hide

  • 個体性

  • 免疫進化

Research Themes 【 Display / hide

  • 棘皮動物ヒトデの免疫システムの解析, 


  • 棘皮動物ヒトデにおける再構築現象の解析, 


  • 棘皮動物ヒトデの変態現象の解析, 



Books 【 Display / hide

  • 動物学の百科事典

    古川 亮平, 丸善出版, 2018.09

    Scope: マクロファージの起源

  • Advances in Comparative Immunology

    Smith, LC., Arriza, V., Barela Hudgell, MA., Barone, G., Bodnar, AG., Buckley, KM., Cunsolo, V., Dheilly, N., Franchi, N., Fugmann, SD., Furukawa, R., Garcia-Arraras, J., Henson, JH., Hibino, T., Irons, ZH., Li, C., Lun, CM., Majeske, AJ., Oren, M., Pagliara, P., Pinsino, A., Raftos, DA., Rast, JP., Samasa, B., Schillaci, D., Schrankel, CS., Stabili, L., Stensväg, K., Sutton, E., Springer International Publishing, 2018.07

    Scope: Echinodermata: The Complex Immune System in Echinoderms.

  • 実験医学別冊 RNA-Seq実験ハンドブック

    大桃 秀樹、古川 亮平、清水 厚志, 羊土社, 2016.03

    Scope: ヒト血液検体のトランスクプトーム解析

  • 実験医学増刊 エピゲノム研究 就職の全体像の理解から先制・個別化医療へ

    古川 亮平、八谷 剛史、清水 厚志, 羊土社, 2016

    Scope: 全エピゲノム関連解析(EWAS)

Papers 【 Display / hide

  • Sex-inducing effects toward planarians widely present among parasitic flatworms

    Sekii K., Miyashita S., Yamaguchi K., Saito I., Saito Y., Manta S., Ishikawa M., Narita M., Watanabe T., Ito R., Taguchi M., Furukawa R., Ikeuchi A., Matsuo K., Kurita G., Kumagai T., Shirakashi S., Ogawa K., Sakamoto K., Koyanagi R., Satoh N., Sasaki M., Maezawa T., Ichikawa-Seki M., Kobayashi K.

    iScience (iScience)  26 ( 1 ) 105776 2023.01

     View Summary

    Various parasitic flatworms infect vertebrates for sexual reproduction, often causing devastating diseases in their hosts. Consequently, flatworms are of great socioeconomic and biomedical importance. Although the cessation of parasitic flatworm sexual reproduction is a major target of anti-parasitic drug design, little is known regarding bioactive compounds controlling flatworm sexual maturation. Using the planarian Dugesia ryukyuensis, we observed that sex-inducing substances found in planarians are also widespread in parasitic flatworms, such as monogeneans and flukes (but not in tapeworms). Reverse-phase HPLC analysis revealed the sex-inducing substance(s) eluting around the tryptophan retention time in the fluke Calicophoron calicophorum, consistent with previous studies on the planarian Bipalium nobile, suggesting that the substance(s) is likely conserved among flatworms. Moreover, six of the 18 ovary-inducing substances identified via transcriptome and metabolome analyses are involved in purine metabolism. Our findings provide a basis for understanding and modifying the life cycles of various parasitic flatworms.

  • Establishment of the immunological self in juvenile Patiria pectinifera post-metamorphosis

    Taguchi M., Minakata K., Tame A., Furukawa R.

    Frontiers in Immunology (Frontiers in Immunology)  13   1056027 2022.12

    ISSN  1664-3224

     View Summary

    Ontogeny of the immune system is a fundamental immunology issue. One indicator of immune system maturation is the establishment of the immunological self, which describes the ability of the immune system to distinguish allogeneic individuals (allorecognition ability). However, the timing of immune system maturation during invertebrate ontogeny is poorly understood. In the sea star Patiria pectinifera, cells that have dissociated from the embryos and larvae are able to reconstruct larvae. This reconstruction phenomenon is possible because of a lack of allorecognition capability in the larval immune system, which facilitates the formation of an allogeneic chimera. In this study, we revealed that the adult immune cells of P. pectinifera (coelomocytes) have allorecognition ability. Based on a hypothesis that allorecognition ability is acquired before and after metamorphosis, we conducted detailed morphological observations and survival time analysis of metamorphosis-induced chimeric larvae. The results showed that all allogeneic chimeras died within approximately two weeks to one month of reaching the juvenile stage. In these chimeras, the majority of the epidermal cell layer was lost and the mesenchymal region expanded, but cell death appeared enhanced in the digestive tract. These results indicate that the immunological self of P. pectinifera is established post-metamorphosis during the juvenile stage. This is the first study to identify the timing of immune system maturation during echinodermal ontogenesis. As well as establishing P. pectinifera as an excellent model for studies on self- and non-self-recognition, this study enhances our understanding of the ontogeny of the immune system in invertebrates.

  • Evaluation of short-term epigenetic age fluctuation

    Komaki S., Ohmomo H., Hachiya T., Sutoh Y., Ono K., Furukawa R., Umekage S., Otsuka-Yamasaki Y., Minabe S., Takashima A., Tanno K., Sasaki M., Shimizu A.

    Clinical Epigenetics (Clinical Epigenetics)  14 ( 1 ) 76 2022.12

    ISSN  18687075

     View Summary

    Considerable effort has been spent on lowering and maintaining the epigenetic age. However, the extent to which epigenetic age fluctuates under normal conditions is poorly understood. Therefore, we analyzed methylation data from monocytes and peripheral blood mononuclear cells collected from two Japanese men. The ranges of the Pan-tissue, Skin and blood, and DNAm PhenoAge epigenetic age during 3 months were ≥ 5.62, ≥ 3.04, and ≥ 8.23 years, and the maximum daily changes were 5.21, 3.20, and 6.53 years, respectively. These fluctuations were not suppressed by correcting for cell-type composition. Although the underlying biological mechanism remains unclear, there was a nonnegligible degree of age fluctuation which should inform personalized clinical applications.

  • Longitudinal DNA methylation dynamics as a practical indicator in clinical epigenetics.

    Komaki, S., Ohmomo, H., Hachiya, T., Sutoh Y., Ono, K., Furukawa, R., Umekage, S., Otsuka-Yamasaki, Y., Tanno, K., Sasaki, M., Shimizu. A.

    Clinical Epigenetics (Clinical Epigenetics)  13 ( 1 ) 219 2021.12

    Joint Work, Accepted,  ISSN  18687075

     View Summary

    Background: One of the fundamental assumptions of DNA methylation in clinical epigenetics is that DNA methylation status can change over time with or without interplay with environmental and clinical conditions. However, little is known about how DNA methylation status changes over time under ordinary environmental and clinical conditions. In this study, we revisited the high frequency longitudinal DNA methylation data of two Japanese males (24 time-points within three months) and characterized the longitudinal dynamics. Results: The results showed that the majority of CpGs on Illumina HumanMethylation450 BeadChip probe set were longitudinally stable over the time period of three months. Focusing on dynamic and stable CpGs extracted from datasets, dynamic CpGs were more likely to be reported as epigenome-wide association study (EWAS) markers of various traits, especially those of immune- and inflammatory-related traits; meanwhile, the stable CpGs were enriched in metabolism-related genes and were less likely to be EWAS markers, indicating that the stable CpGs are stable both in the short-term within individuals and under various environmental and clinical conditions. Conclusions: This study indicates that CpGs with different stabilities are involved in different functions and traits, and thus, they are potential indicators that can be applied for clinical epigenetic studies to outline underlying mechanisms.

  • dbTMM: an integrated database of large-scale cohort, genome and clinical data for the Tohoku Medical Megabank Project

    Ogishima S., Nagaie S., Mizuno S., Ishiwata R., Iida K., Shimokawa K., Takai-Igarashi T., Nakamura N., Nagase S., Nakamura T., Tsuchiya N., Nakaya N., Murakami K., Ueno F., Onuma T., Ishikuro M., Obara T., Mugikura S., Tomita H., Uruno A., Kobayashi T., Tsuboi A., Tadaka S., Katsuoka F., Narita A., Sakurai M., Makino S., Tamiya G., Aoki Y., Shimizu R., Motoike I.N., Koshiba S., Minegishi N., Kumada K., Nobukuni T., Suzuki K., Danjoh I., Nagami F., Tanno K., Ohmomo H., Asahi K., Shimizu A., Hozawa A., Kuriyama S., Yamamoto M., Abe M., Aizawa Y., Aoki Y., Chida K., Egawa S., Eto A., Funayama T., Fuse N., Hamanaka Y., Harada Y., Hashizume H., Higuchi S., Hirano S., Hirata T., Hiratsuka M., Hozawa A., Igarashi K., Inoue J., Ishida N., Ishii N., Ishii T., Ishikuro M., Ito K., Ito S., Kageyama M., Katsuoka F., Kawame H., Kawashima J., Kikuya M., Kinoshita K., Kitatani K., Kiyama T., Kiyomoto H., Kobayashi T., Kodama E., Kogure M., Kojima K., Koreeda S., Koshiba S., Koyama S., Kudo H., Kumada K., Kure S., Kuriki M., Kuriyama S., Kuroki Y., Maikusa N., Makino S., Matsubara H., Matsui H., Metoki H., Mimori T., Misawa K.

    Human Genome Variation (Human Genome Variation)  8 ( 1 )  2021.12

     View Summary

    To reveal gene-environment interactions underlying common diseases and estimate the risk for common diseases, the Tohoku Medical Megabank (TMM) project has conducted prospective cohort studies and genomic and multiomics analyses. To establish an integrated biobank, we developed an integrated database called “dbTMM” that incorporates both the individual cohort/clinical data and the genome/multiomics data of 157,191 participants in the Tohoku Medical Megabank project. To our knowledge, dbTMM is the first database to store individual whole-genome data on a variant-by-variant basis as well as cohort/clinical data for over one hundred thousand participants in a prospective cohort study. dbTMM enables us to stratify our cohort by both genome-wide genetic factors and environmental factors, and it provides a research and development platform that enables prospective analysis of large-scale data from genome cohorts.

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Papers, etc., Registered in KOARA 【 Display / hide

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Reviews, Commentaries, etc. 【 Display / hide

  • Echinodermata: The complex immune system in echinoderms (Advances in Comparative Immunology, 10.1007/978-3-319-76768-0_13)

    Smith L., Arizza V., Hudgell M., Barone G., Bodnar A., Buckley K., Cunsolo V., Dheilly N., Franchi N., Fugmann S., Furukawa R., Garcia-Arraras J., Henson J., Hibino T., Irons Z., Li C., Lun C., Majeske A., Oren M., Pagliara P., Pinsino A., Raftos D., Rast J., Samasa B., Schillaci D., Schrankel C., Stabili L., Stensv K., Sutton E.

    Coresource 1 (Coresource 1)   2018.01

     View Summary

    © Springer International Publishing AG, part of Springer Nature 2018. This chapter was inadvertently published with an incorrect spelling of the author's name as V. Arriza whereas it should be V. Arizza. In addition to this the affiliation of one of the chapter authors Elisse Sutton was published incorrectly and it has now been corrected to read as Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia.

  • IMMオミックスリファレンスパネルの取り組み

    古川 亮平、清水 厚志

    生化学 88 ( 1 ) 36 - 43 2016

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

Presentations 【 Display / hide

  • イトマキヒトデ成体の免疫細胞における細胞外小胞の分泌と取り込み

    南方宏太, 田口瑞姫, 多米晃裕, 倉石立, 古川亮平



    Oral presentation (general)

  • イトマキヒトデの個体生確立メカニズムの解明を目指した時系列1個体RNA-seq解析

    田口瑞姫, 古川亮平

    日本比較免疫学会第 34 回学術集会, 


    Oral presentation (general)

  • Prediction of the process for the immune system maturation in the sea star, Patiria pectinifera

    Mizuki Taguchi, Kota Minakata, Haruka Sato, Akihiro Tame, Ryohei Furukawa

    15th Congress of the International Society for Developmental and Comparative Immunology, 


    Poster presentation

  • イトマキヒトデ成体における免疫応答時の体腔液成分の分析




    Oral presentation (general)

  • 自家蛍光を持つ成体ホヤ血球細胞の発生学的起源と分類




    Oral presentation (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • イトマキヒトデの変態誘導機構の解明


    Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), No Setting

     View Summary


  • ヒトデ幼生の自己・非自己認識に関わる種特異的糖鎖の探索


    Grants-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists, Principal investigator

     View Summary

    申請者の先行研究により、棘皮動物イトマキヒトデの幼生において唯一の免疫細胞である間充織細胞が、同種異個体のみならず本来幼生の体内に存在しない精 子であっても、生きた同種の細胞であれば免疫応答を生じない「missing-species(種の喪失)認識」によって自己と非自己を識別している可能性が示唆されている。この認識システムを可能にするには、自己マーカーに相当する種特異的な分子の存在が必要であるが、予備データからこの分子が何らかの糖鎖であることが示唆されていた。本研究は、イトマキヒトデの精子を材料に、幼生のmissing-species認識を可能にする種特異的な糖鎖を探索し、その単離・同定を目指すものである。
    今年度はまず、各種レクチンを用いたスクリーニングの再現性の確認を行った。昨年度と同様、各種レクチンを結合させた生きた精子を幼生体内に顕微注射し、 間充織細胞による貪食作用の有無を調べた。昨年度はConA結合精子に対する貪食作用の増強が認められたが、コントロールと比較して統計的に有意な差は得られなかった。同様に、その他のレクチンで染色した精子を用いた場合においても貪食作用が認められるケースもあった。従って、レクチン染色時の操作による精子の生存率の低下によって、死細胞に対する免疫応答の活性化として検出された可能性が排除できない。一方、各種糖鎖切断酵素で処理した精子の生存率も高いとは言えず、昨年度に引き続いて条件検討は難航している。

  • 海洋プランクトン幼生の成長:摂餌の消化・吸収と栄養素の伝播・受容


    Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Coinvestigator(s)

     View Summary

    最近、間充織細胞のMC5分子はTGFbを活性化させて上皮増殖を誘起することを証明できた。 これに加え、MC5分子は上皮細胞の生存にも関与している可能性も見出した。モルフォリノオリゴ(MO)でMC5分子の発現を抑制させたサンプルでは、アポトーシス細胞死が生じている現場として上皮細胞の断片化が、電子顕微鏡下で検出され、上記の可能性が支持された(TGFbが上皮細胞の生存に関与してないことは、TGFb-MOサンプルで確認した)。また、ペプチド性のアポトーシス阻害剤とMC5-MOの同時注射により、MC5-MOサンプルに特徴的な著しい細胞数の減少が緩和されるという強力な補助データも得られた。

  • DNAメチル化・トランスクリプトーム・血漿メタボロームの3層オミックス解析を用いた電気加熱式たばこの喫煙・受動喫煙の県境影響に関する疫学研究


    日本医療研究開発機構, 循環器疾患・糖尿病等生活習慣病対策実用化研究事業, Other, Coinvestigator(s)

  • Regulatory system of inflammation by two macrophage migration inhibitory factors in starfish larvae


    Grant-in-Aid for Scientific Research, FURUKAWA Ryohei, Grant-in-Aid for Young Scientists (B), Research grant, Principal investigator

     View Summary

    Macrophage migration inhibitory factor (MIF) is thought to be one of the most primitive cytokines in phylogenetic evolution. In this study, to understand the regulatory system of inflammation by MIF in starfish larvae, we searched the receptor(s) of two types of MIF (ApMIF1 and ApMIF2) and the downstream signaling pathway(s) from the immune cells. RNA-seq analysis in the larval immune cells treated with two recombinant MIFs suggested that MAPK and PI3K/Akt signaling pathways exist downstream of MIFs. Furthermore, two types of G protein-coupled receptors and two types of receptor tyrosine kinases were obtained as receptor candidates. These results provide useful insights for understanding the evolutionary conservation and diversity of regulatory system of MIF-mediated inflammation.

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Awards 【 Display / hide

  • 日本比較免疫学会 古田優秀論文賞

    古川 亮平, 2016.08, 日本比較免疫学会, 2つのマクロファージ遊走阻止因子がヒトデ幼生の間充織細胞の走化性を制御する

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • 日本比較免疫学会 古田奨励賞

    FURUKAWA Ryohei, 2009.08, ヒトデ胚の間充織細胞におけるApDOCKタンパク質の構造及び発現解析

    Type of Award: Award from Japanese society, conference, symposium, etc.


Courses Taught 【 Display / hide











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Memberships in Academic Societies 【 Display / hide

  • 日本比較免疫学会

  • 日本動物学会


Committee Experiences 【 Display / hide

  • 2016.09

    役員, 日本比較免疫学会