KEIO RESEARCHERS INFORMATION SYSTEM

Career 【 Display / hide 】

Career 【 Display / hide 】

• 2012.04

  -

  2012.06

  東京大学　理学系研究科　物理学専攻

• 2012.07

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  2015.06

  ミュンヘン工科大学　物理学科

• 2015.07

  -

  2017.03

  京都大学　物質－細胞統合システム拠点

• 2017.04

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  2018.03

  京都大学 医学研究科

• 2018.04

  -

  2024.03

  京都大学　高等研究院　医学物理・医工計測グローバル拠点

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Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 2003.04

  -

  2007.03

  International Christian University, 教養学部, 理学科

  University, Graduated

• 2007.04

  -

  2009.03

  The University of Tokyo, 理学系研究科, 物理学専攻

  Graduate School, Completed, Master's course

• 2009.04

  -

  2012.03

  The University of Tokyo, 理学系研究科, 物理学専攻

  Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• 博士（理学）, The University of Tokyo, Coursework, 2012.03

Research Areas 【 Display / hide 】

Research Areas 【 Display / hide 】

• Natural Science / Biophysics, chemical physics and soft matter physics (アクティブマター物理、生命物理、医学物理)

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Extracellular calcium functions as a molecular glue for transmembrane helices to activate the scramblase Xkr4

  Zhang, Panpan, Maruoka, Masahiro, Suzuki, Ryo, Katani, Hikaru, Dou, Yu, Packwood, Daniel M., Kosako, Hidetaka, Tanaka, Motomu and Suzuki, Jun

  Nature Communications 14 ( 1 ) 5592 2023

  ISSN  2041-1723

  　View Summary

  The “eat me” signal, phosphatidylserine is exposed on the surface of dying cells by phospholipid scrambling. Previously, we showed that the Xkr family protein Xkr4 is activated by caspase-mediated cleavage and binding of the XRCC4 fragment. Here, we show that extracellular calcium is an additional factor needed to activate Xkr4. The constitutively active mutant of Xkr4 is found to induce phospholipid scrambling in an extracellular, but not intracellular, calcium-dependent manner. Importantly, other Xkr family members also require extracellular calcium for activation. Alanine scanning shows that D123 and D127 of TM1 and E310 of TM3 coordinate calcium binding. Moreover, lysine scanning demonstrates that the E310K mutation-mediated salt bridge between TM1 and TM3 bypasses the requirement of calcium. Cysteine scanning proves that disulfide bond formation between TM1 and TM3 also activates phospholipid scrambling without calcium. Collectively, this study shows that extracellular calcium functions as a molecular glue for TM1 and TM3 of Xkr proteins for activation, thus demonstrating a regulatory mechanism for multi-transmembrane region-containing proteins.

  • Access to Document (DOI)

• Wnt/β-catenin signaling induces axial elasticity patterns of Hydra extracellular matrix

  Veschgini, Mariam, Suzuki, Ryo, Kling, Svenja, Petersen, Hendrik O., Bergheim, Bruno Gideon, Abuillan, Wasim, Linke, Philipp, Kaufmann, Stefan, Burghammer, Manfred, Engel, Ulrike, Stein, Frank, Özbek, Suat, Holstein, Thomas W. and Tanaka, Motomu

  iScience 26 ( 4 )  2023

  ISSN  2589-0042

  • Access to Document (DOI)

• Spatio-temporal Coordination of Active Deformation Forces and Wnt/Hippo-Yap Signaling in Hydra Regeneration

  Suzuki, Ryo, Hiraiwa, Tetsuya, Tursch, Anja, Höger, Stefanie, Hayashi, Kentaro, Özbek, Suat, Holstein, Thomas W and Tanaka, Motomu

  BioRXiv    2023.09. 18.558226 2023

• Ion-specific nanoscale compaction of cysteine-modified poly (acrylic acid) brushes revealed by 3D scanning force microscopy with frequency modulation detection

  Yamamoto, Akihisa, Ikarashi, Takahiko, Fukuma, Takeshi, Suzuki, Ryo, Nakahata, Masaki, Miyata, Kazuki and Tanaka, Motomu

  Nanoscale Advances 4 ( 23 ) 5027 - 5036 2022

• Extreme deformability of insect cell membranes is governed by phospholipid scrambling

  Shiomi, Akifumi, Nagao, Kohjiro, Yokota, Nobuhiro, Tsuchiya, Masaki, Kato, Utako, Juni, Naoto, Hara, Yuji, Mori, Masayuki X., Mori, Yasuo, Ui-Tei, Kumiko, Murate, Motohide, Kobayashi, Toshihide, Nishino, Yuri, Miyazawa, Atsuo, Yamamoto, Akihisa, Suzuki, Ryo, Kaufmann, Stefan, Tanaka, Motomu, Tatsumi, Kazuya, Nakabe, Kazuyoshi, Shintaku, Hirofumi, Yesylevsky, Semen, Bogdanov, Mikhail and Umeda, Masato

  Cell Reports 35 ( 10 ) 109219 2021

  ISSN  2211-1247

  　View Summary

  Summary Organization of dynamic cellular structure is crucial for a variety of cellular functions. In this study, we report that Drosophila and Aedes have highly elastic cell membranes with extremely low membrane tension and high resistance to mechanical stress. In contrast to other eukaryotic cells, phospholipids are symmetrically distributed between the bilayer leaflets of the insect plasma membrane, where phospholipid scramblase (XKR) that disrupts the lipid asymmetry is constitutively active. We also demonstrate that XKR-facilitated phospholipid scrambling promotes the deformability of cell membranes by regulating both actin cortex dynamics and mechanical properties of the phospholipid bilayer. Moreover, XKR-mediated construction of elastic cell membranes is essential for hemocyte circulation in the Drosophila cardiovascular system. Deformation of mammalian cells is also enhanced by the expression of Aedes XKR, and thus phospholipid scrambling may contribute to formation of highly deformable cell membranes in a variety of living eukaryotic cells.

  • Access to Document (DOI)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• 力学的拘束によるヒドラオルガノイドの再生能力制御技術の開拓

  2022

  -

  Present

  科学研究費助成事業, 基盤研究（B）, Principal investigator

• 可逆的に変調可能なしわ基板を用いた筋管形成の動的制御技術の開拓

  2019

  -

  2021

  科学研究費助成事業, 基盤研究（C）, Principal investigator

• 再生ヒドラの揺らぎの全モード解析

  2016

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  2019

  科学研究費助成事業, 若手研究（B）, Principal investigator

• 脊索中胚葉システムの形態形成における自発運動の力学解析

  2016

  -

  2018

  科学研究費助成事業, 基盤研究（B）特設, Coinvestigator(s)

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• TOPICS IN BIOSCIENCES AND INFORMATICS 2

  2025

• SEMINAR IN BIOSCIENCES AND INFORMATICS

  2025

• LABORATORY IN SCIENCE

  2025

• BASIC LABORATORY COURSE IN BIOSCIENCES

  2025

• BACHELOR'S THESIS

  2025

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