Reviews, Commentaries, etc. - Arai, Midori

Ricinus communisなどからのWntシグナルに作用する天然物の探索

OISHI KENSUKE, TOUME KAZUFUMI, ARAI MIDORI, MIZOGUCHI TAKAMASA, ITO MOTOYUKI, SAMIR K SADHU, FIROJ AHMED, KOYANO TAKASHI, THAWORN KOWITHAYAKORN, ISHIBASHI MASAMI

日本薬学会年会要旨集(CD-ROM) 134th   ROMBUNNO.28L-AM06 2014

Artocarpus communisからのTRAIL耐性克服作用を有する天然物の探索

HABU TADASHI, TOUME KAZUFUMI, ARAI MIDORI, KOYANO TAKASHI, KOWITHAYAKORN, THAWORN, ISHIBASHI MASAMI

食品薬学シンポジウム講演要旨集 5th   154 - 156 2013.10

Ricinus communisからのWntシグナル活性化作用をもつ天然物の探索

OISHI KENSUKE, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

食品薬学シンポジウム講演要旨集 5th   151 - 153 2013.10

Calotropis gigantea由来カルデノリドのWnt阻害作用

BOKU KEN'EI, TOUME KAZUFUMI, ARAI MIDORI, SAMIR K. SADHU, FIROJ AHMED, ISHIBASHI MASAMI

日本薬学会年会要旨集(CD-ROM) 133rd   ROMBUNNO.29L-PM05 2013

Saussurea hypoleucaからのTRAIL耐性克服作用を有する天然物の探索

TSUCHII OSAMU, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

日本薬学会年会要旨集(CD-ROM) 133rd   ROMBUNNO.28M-AM09S 2013

Zanthoxylum rhetsaからのWntシグナルを活性化する天然物の探索

OISHI KENSUKE, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

日本薬学会年会要旨集(CD-ROM) 133rd   ROMBUNNO.29AMA-266 2013

Xylocarpus granatumの新規リモノイドのWntシグナル阻害作用

KAMIYA KENTARO, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

日本薬学会年会要旨集(CD-ROM) 133rd   ROMBUNNO.29AMA-280 2013

Rapid Search for Bioactive Natural Products using Stem Cell Transcription Factor Hes1 Beads

Arai Midori A., Ishikawa Naoki, Koyano Takashi, Thaworn Kowithayakorn, Ishibashi Masami

Symposium on the Chemistry of Natural Products, symposium papers (Symposium on the Chemistry of Natural Products Steering Committee)  55 ( 0 ) Oral17 2013

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<p>Basic helix-loop-helix (bHLH) repressor- and activator-type transcription factors play essential roles in proliferation and differentiation of neural stem cells (NSCs). Hes1, one of bHLH repressor-type factors, suppresses transcriptions of bHLH activator-type factors such as Mash1 by binding to their promoter regions as homodimers, which leads NSCs to remaining undifferentiation. Therefore, inhibition of Hes1 dimer formation would accelerate NSCs differentiation into neurons and glial cells. To quickly find ligands of bHLH factors from natural products extracts, we constructed Hes1 immobilized sepharose beads-HPLC method. Flavonoid glycosides (1-3), xanthone derivative (4) and polyene macrololactam (5) were isolated rapidly by this method.</p><p>Compound 1 disrupted Hes1 dimer formation in mouse embryo fibroblast cells (C3H10T1/2), and moreover, 1 suppressed Hes1-mediated repression of bHLH activator-type transcription factor dose-dependently (reporter assay). In differentiation of mouse neural stem cells (MEB5), neurite outgrowth enhancement by 1 was clearly observed. The expression of pro-neural genes (activator-type bHLH transcription genes) such as Mash1 and NeuroD were increased by treatment of MEB5 with 1compared with those of control. These results suggested that the inhibition of Hes1 dimer formation by 1 would greatly contribute its mode-of-action. </p><p>Compound 4 inhibited Hes1-mediated suppression of gene expression in reporter assay, although Hes1 dimer inhibition was not detected. The mechanism of 4 would be different with that of 1.</p>

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TRAIL耐性克服作用をもつ天然物の探索:Artocarpus champedenのプレニルフラボン成分

MINAKAWA TOMOHIRO, TOUME KAZUFUMI, ARAI MIDORI, KOYANO TAKASHI, KOWITHAYAKORN, THAWORN, ISHIBASHI MASAMI

香料・テルペンおよび精油化学に関する討論会講演要旨集 56th   331 - 333 2012.10

Calotropis giganteaからのWntシグナル阻害作用をもつカルデノリド

PARK HYUN, YOUNG, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

天然薬物の開発と応用シンポジウム講演要旨集 19th   88 - 90 2012.10

ISSN  0919-2085

Xylocarpus granatumからのWntシグナル阻害作用をもつ天然化合物の探索

KAMIYA KENTARO, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

天然薬物の開発と応用シンポジウム講演要旨集 19th   85 - 87 2012.10

ISSN  0919-2085

Artocarpus communis等からのTRAIL耐性克服作用を有する天然物の探索

HABU OSAMU, TOUME KAZUFUMI, ARAI MIDORI, KOYANO TAKASHI, KOWITHAYAKORN THAWORN, SAMIR SADHU, FIROJ AHMED, ISHIBASHI MASAMI

日本生薬学会年会講演要旨集 59th   155 2012.08

ISSN  0919-1992

Calotropis gigantea由来のWntシグナル阻害するカルデノライド

BOKU KEN'EI, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

日本生薬学会年会講演要旨集 59th   220 2012.08

ISSN  0919-1992

Xylocarpus granatum由来のWntシグナル阻害作用をもつ新規リモノイド

KAMIYA KENTARO, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

日本生薬学会年会講演要旨集 59th   222 2012.08

ISSN  0919-1992

Artocarpus champedenからのTRAIL耐性克服作用を有する天然物の探索

MINAGAWA TOMOHIRO, TOUME KAZUFUMI, ARAI MIDORI, KOYANO TAKASHI, THAWORN KOWITHAYAKORN, ISHIBASHI MASAMI

日本薬学会年会要旨集 132nd ( 2 ) 174 2012.03

ISSN  0918-9823

Artocarpus communisからのTRAIL耐性克服作用を有する天然物の探索

TSUCHIO OSAMU, TOUME KAZUFUMI, ARAI MIDORI, KOYANO TAKASHI, THAWORN KOWITHAYAKORN, ISHIBASHI MASAMI

日本薬学会年会要旨集 132nd ( 2 ) 174 2012.03

ISSN  0918-9823

Wntシグナル阻害作用をもつXylocarpus granatum由来の新規リモノイド

KAMIYA KENTARO, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

日本薬学会年会要旨集 132nd ( 2 ) 173 2012.03

ISSN  0918-9823

8 New Bioactive Aromatic Heterocycles from Streptomyces spp. Collected in Chiba(Oral Presentation)

Abdelfattah Mohamed S., Toume Kazufumi, Arai Midori A., Ishibashi Masami

Symposium on the Chemistry of Natural Products, symposium papers (Symposium on the Chemistry of Natural Products Steering Committee)  54 ( 0 ) 43 - 48 2012

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During our studies on search for bioactive natural products from various sources, we have investigated bioactive metabolites of actinomycete strains collected several places in Chiba area, and isolated new bioactive aromatic heterocyclic natural products, including izumiphenazines A-D (1-4) from the cultured broth of Streptomyces sp. IFM 11204 isolated soil sample of Izumi-forest in Chiba city, izuminosides A-C (9-11) from Streptomyces sp. IFM 11260 collected also in Izumi-forest in Chiba city, new pyranonaphthoquinones (12-15) yorophenazine (16), and yoropyrazone (17) from Streptomyces sp. IFM 11307 collected from Yoro-valley, and katorazone (18) from Streptomyces sp. IFM 11299 collected from Katori city. The structures of new compounds were elucidated on the basis of spectral data, and their TRAIL resistance overcoming activity was evaluated to reveal that compound 12 at 0.1 μM proved to be the most active in this series, with 33% decrease in cell viability in the presence of TRAIL (100 ng/mL).

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ピロン環およびビスインドール骨格を含む変形菌の化学成分

ISHIBASHI MASAMI, SHINTANI AKINORI, TOUME KAZUFUMI, ARAI MIDORI

複素環化学討論会講演要旨集 41st   356 - 357 2011.09

Calotropis giganteaからのWntシグナル阻害作用をもつ天然物の探索

BOKU KEN'EI, TOUME KAZUFUMI, ARAI MIDORI, SADHU SAMIR K, AHMED FIROJ, ISHIBASHI MASAMI

日本生薬学会年会講演要旨集 58th   216 2011.09

ISSN  0919-1992