KEIO RESEARCHERS INFORMATION SYSTEM

Other Affiliation 【 Display / hide 】

Other Affiliation 【 Display / hide 】

• 自然科学研究教育センター

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• 博士（水産学）, 北里大学, Coursework, 2019.03

  マナマコ体腔細胞の凝集反応に関する研究

Licenses and Qualifications 【 Display / hide 】

Licenses and Qualifications 【 Display / hide 】

• 中学校教諭専修免許　理科, 2016.03

• 高等学校教諭専修免許　理科, 2016.03

Research Areas 【 Display / hide 】

Research Areas 【 Display / hide 】

• Life Science / Aquatic life science

• Life Science / Molecular biology

• Life Science / Cell biology

• Life Science / Developmental biology

• Life Science / Evolutionary biology

Research Keywords 【 Display / hide 】

Research Keywords 【 Display / hide 】

• 免疫細胞

• 創発

• 棘皮動物

• 細胞凝集

• 細胞分化

Research Themes 【 Display / hide 】

Research Themes 【 Display / hide 】

• 棘皮動物ナマコにおける造血器官の探索, 

  2023.04

  -

  Present

• 棘皮動物ヒトデの変態現象の解析, 

  2020.04

  -

  Present

• 棘皮動物ヒトデの免疫システムの解析, 

  2019.04

  -

  Present

• 棘皮動物ナマコの腸排出時に形成される凝集塊の機能解析, 

  2016.04

  -

  Present

• 棘皮動物ナマコにおける細胞凝集反応の解析, 

  2013.04

  -

  Present

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Sex-inducing effects toward planarians widely present among parasitic flatworms

  Sekii K., Miyashita S., Yamaguchi K., Saito I., Saito Y., Manta S., Ishikawa M., Narita M., Watanabe T., Ito R., Taguchi M., Furukawa R., Ikeuchi A., Matsuo K., Kurita G., Kumagai T., Shirakashi S., Ogawa K., Sakamoto K., Koyanagi R., Satoh N., Sasaki M., Maezawa T., Ichikawa-Seki M., Kobayashi K.

  iScience 26 ( 1 )  2023.01

  　View Summary

  Various parasitic flatworms infect vertebrates for sexual reproduction, often causing devastating diseases in their hosts. Consequently, flatworms are of great socioeconomic and biomedical importance. Although the cessation of parasitic flatworm sexual reproduction is a major target of anti-parasitic drug design, little is known regarding bioactive compounds controlling flatworm sexual maturation. Using the planarian Dugesia ryukyuensis, we observed that sex-inducing substances found in planarians are also widespread in parasitic flatworms, such as monogeneans and flukes (but not in tapeworms). Reverse-phase HPLC analysis revealed the sex-inducing substance(s) eluting around the tryptophan retention time in the fluke Calicophoron calicophorum, consistent with previous studies on the planarian Bipalium nobile, suggesting that the substance(s) is likely conserved among flatworms. Moreover, six of the 18 ovary-inducing substances identified via transcriptome and metabolome analyses are involved in purine metabolism. Our findings provide a basis for understanding and modifying the life cycles of various parasitic flatworms.

  • Access to Document (DOI)

• Establishment of the immunological self in juvenile Patiria pectinifera post-metamorphosis

  Taguchi M., Minakata K., Tame A., Furukawa R.

  Frontiers in Immunology 13 2022.12

  　View Summary

  Ontogeny of the immune system is a fundamental immunology issue. One indicator of immune system maturation is the establishment of the immunological self, which describes the ability of the immune system to distinguish allogeneic individuals (allorecognition ability). However, the timing of immune system maturation during invertebrate ontogeny is poorly understood. In the sea star Patiria pectinifera, cells that have dissociated from the embryos and larvae are able to reconstruct larvae. This reconstruction phenomenon is possible because of a lack of allorecognition capability in the larval immune system, which facilitates the formation of an allogeneic chimera. In this study, we revealed that the adult immune cells of P. pectinifera (coelomocytes) have allorecognition ability. Based on a hypothesis that allorecognition ability is acquired before and after metamorphosis, we conducted detailed morphological observations and survival time analysis of metamorphosis-induced chimeric larvae. The results showed that all allogeneic chimeras died within approximately two weeks to one month of reaching the juvenile stage. In these chimeras, the majority of the epidermal cell layer was lost and the mesenchymal region expanded, but cell death appeared enhanced in the digestive tract. These results indicate that the immunological self of P. pectinifera is established post-metamorphosis during the juvenile stage. This is the first study to identify the timing of immune system maturation during echinodermal ontogenesis. As well as establishing P. pectinifera as an excellent model for studies on self- and non-self-recognition, this study enhances our understanding of the ontogeny of the immune system in invertebrates.

  • Access to Document (DOI)

• Lineage-specific evolution of zona pellucida genes in fish

  Sano K., Shimada S., Mibu H., Taguchi M., Ohsawa T., Kawaguchi M., Yasumasu S.

  Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 338 ( 3 ) 181 - 191 2022.05

  ISSN  15525007

  　View Summary

  The zona pellucida (ZP) protein constitutes the egg envelope, which surrounds the vertebrate embryo. We performed a comprehensive study on the molecular evolution of ZP genes in Teleostei by cloning and analyzing the expression of ZP genes in fish of Anguilliformes in Elopomorpha, Osteoglossiformes in Osteoglossomorpha, and Clupeiformes in Otocephala to cover unsurveyed fish groups in Teleostei. The present results confirmed findings from our previous reports that the principal organ of ZP gene expression changed from ovary to liver in the common ancestors of Clupeocephala. Even fish species that synthesize egg envelopes in the liver carry the ovary-expressed ZP proteins as minor egg envelope components that were produced by gene duplication during the early stage of Teleostei evolution. The amino acid repeat sequences located at the N-terminal region of ZP proteins are known to be the substrates of transglutaminase responsible for egg envelope hardening and hatching. A repeat sequence was found in zona pellucida Cs of phylogenetically early diverged fish. After changing the synthesis organ, its role is inherited by the N-terminal Pro-Gln-Xaa repeat sequence in liver-expressed zona pellucida B genes of Clupeocephala. These results suggest that teleost ZP genes have independently evolved to maintain fish-specific functions, such as egg envelope hardening and egg envelope digestion, at hatching.

  • Access to Document (DOI)

• Galactose-Binding C-Type Lectin Promotes Cellular Aggregation of Coelomocytes in Sea Cucumber

  Taguchi M., Tanaka C., Tsutsui S., Nakamura O.

  Frontiers in Immunology 12 2021.12

  　View Summary

  Echinoderms have a large coelomic cavity containing coelomocytes. When the coelomic fluid is removed from the cavity, the cells aggregate immediately. We found that a fraction or an extract of the intestine of the sea cucumber, Apostichopus japonicus, markedly accelerated cellular movement and aggregation on a glass slide, and this effect was clearly inhibited by galactose. We successfully purified the aggregation-promoting factor, a 16 kDa protein, from the intestine. TOF-MS analysis followed by de novo sequencing revealed that the protein is a C-type lectin. RNA-seq data and cDNA cloning demonstrated the factor to be a novel lectin, named AjGBCL, consisting of 158 aa residues in the mature form. Microscopic observation revealed that most of the aggregating cells moved toward aggregates and not to an intestinal fragment, suggesting that AjGBCL is not a chemoattractant but a cellular aggregation-inducing factor that may induce aggregates to release chemoattractant. We report, for the first time, an endogenous molecule that promotes coelomocyte aggregation in echinoderms.

  • Access to Document (DOI)

• Differential count and time-course analysis of the cellular composition of coelomocyte aggregate of the Japanese sea cucumber Apostichopus japonicus

  Fish & Shellfish Immunology 58   203 - 209 2016

  Research paper (scientific journal), Joint Work, Lead author, Accepted

  • Access to Document (DOI)

Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Identification of macrophage migration inhibitory factor receptor in Patiria pectinifera.

  Taguchi, Mizuki

  学事振興資金研究成果実績報告書 (慶應義塾大学)  2021

• Dynamics of alloreactive immune response in adult starfish, Patiria pectinifera

  Taguchi, Mizuki

  学事振興資金研究成果実績報告書 (慶應義塾大学)  2020

Presentations 【 Display / hide 】

Presentations 【 Display / hide 】

• アカガイScapharca broughtonii血球の形態学的および機能的分類の試み

  伊藤篤子,上野天馬,田口瑞姫,古川亮平

  日本動物学会 第95回大会, 

  2024.09

  , 

  Oral presentation (general)

• ヒトデの変態をトリガーする自己非自己間相互作用とその下流シグナル

  田口瑞姫,古川亮平

  日本比較免疫学会 第35回学術集会, 

  2024.08

  , 

  Symposium, workshop panel (nominated)

• イトマキヒトデ成体の体腔細胞に由来する無核の細胞断片は細胞外小胞の供給源の一つである

  南方宏太,田口瑞姫,倉石立,古川亮平

  日本比較免疫学会第35回学術集会, 

  2024.08

  , 

  Oral presentation (general)

• イトマキヒトデの個体性確立メカニズムの解明を目指した時系列1個体RNA-seq解析

  田口瑞姫,古川亮平

  日本比較免疫学会第34回学術集会, 

  2023.08

  , 

  Oral presentation (general)

• MIF homolog induces chemotaxis of coelomocytes of the Japanese sea cucumber Apostichopus japonicus

  13. Sato, M., Sugawara, S., Taguchi, M., Tsutsui, S., Nakamura, O

  (Wageningen) , 

  2023.08

  , 

  Oral presentation (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• イトマキヒトデの変態誘導機構の解明

  2023.04

  -

  2026.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤C, Coinvestigator(s)

• マナマコ体腔細胞が形成する微小環境の特性解析

  2022.04

  -

  2025.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, 若手研究, Principal investigator

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• 日本比較免疫学会 古田優秀論文賞

  2022.08

• 第33回北里大学同窓会研究奨励賞

  2022.05

• 日本比較免疫学会 古田トラベルアワード受賞

  2018.08