大場 純奈 (オオバ ジュンナ)

Oba, Junna

写真a

所属(所属キャンパス)

医学部 石井・石橋記念講座(拡張知能医学) (信濃町)

職名

専任講師(有期)

HP

特記事項

旧姓:岡田

経歴 【 表示 / 非表示

  • 2022年04月
    -
    継続中

    慶應義塾大学医学部, 石井・石橋記念講座(拡張知能医学), 専任講師

  • 2021年11月
    -
    2022年03月

    慶應義塾大学医学部, 石井・石橋記念講座(拡張知能医学), 助教

  • 2019年10月
    -
    2021年10月

    慶應義塾大学医学部, 腫瘍センター ゲノム医療ユニット, 特任講師

  • 2018年05月
    -
    2019年09月

    テキサス大学MDアンダーソンがんセンター, メラノーマ腫瘍学, インストラクター

  • 2016年07月
    -
    2018年04月

    テキサス大学MDアンダーソンがんセンター, メラノーマ腫瘍学, 上席主任研究官

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学歴 【 表示 / 非表示

  • 1996年04月
    -
    2003年03月

    熊本大学, 医学部

    大学, 卒業, その他

  • 2007年04月
    -
    2012年03月

    九州大学, 大学院医学系学府

    大学院, 卒業, 博士

学位 【 表示 / 非表示

  • 医師, 熊本大学医学部, 課程, 2003年04月

  • 博士(医学), 九州大学大学院医学系学府, 課程, 2012年03月

免許・資格 【 表示 / 非表示

  • 医師免許, 2003年04月

  • 日本皮膚科学会専門医, 2009年10月

 

論文 【 表示 / 非表示

  • Multi-modal molecular programs regulate melanoma cell state

    Andrews M.C., Oba J., Wu C.J., Zhu H., Karpinets T., Creasy C.A., Forget M.A., Yu X., Song X., Mao X., Robertson A.G., Romano G., Li P., Burton E.M., Lu Y., Sloane R.S., Wani K.M., Rai K., Lazar A.J., Haydu L.E., Bustos M.A., Shen J., Chen Y., Morgan M.B., Wargo J.A., Kwong L.N., Haymaker C.L., Grimm E.A., Hwu P., Hoon D.S.B., Zhang J., Gershenwald J.E., Davies M.A., Futreal P.A., Bernatchez C., Woodman S.E.

    Nature Communications 13 ( 1 ) 4000 2022年07月

    研究論文(学術雑誌), 共著, 筆頭著者, 査読有り

     概要を見る

    Melanoma cells display distinct intrinsic phenotypic states. Here, we seek to characterize the molecular regulation of these states using multi-omic analyses of whole exome, transcriptome, microRNA, long non-coding RNA and DNA methylation data together with reverse-phase protein array data on a panel of 68 highly annotated early passage melanoma cell lines. We demonstrate that clearly defined cancer cell intrinsic transcriptomic programs are maintained in melanoma cells ex vivo and remain highly conserved within melanoma tumors, are associated with distinct immune features within tumors, and differentially correlate with checkpoint inhibitor and adoptive T cell therapy efficacy. Through integrative analyses we demonstrate highly complex multi-omic regulation of melanoma cell intrinsic programs that provide key insights into the molecular maintenance of phenotypic states. These findings have implications for cancer biology and the identification of new therapeutic strategies. Further, these deeply characterized cell lines will serve as an invaluable resource for future research in the field.

  • SARS-CoV-2 RT-qPCR testing of pooled saliva samples: A case study of 824 asymptomatic individuals and a questionnaire survey in Japan

    Oba J., Taniguchi H., Sato M., Takanashi M., Yokemura M., Sato Y., Nishihara H.

    PLoS ONE 17 ( 5 May ) e0263700 2022年05月

    研究論文(学術雑誌), 共著, 筆頭著者, 査読有り

     概要を見る

    From the beginning of the COVID-19 pandemic, the demand for diagnostic and screening tests has exceeded supply. Although the proportion of vaccinated people has increased in wealthier countries, breakthrough infections have occurred amid the emergence of new variants. Pooled-sample COVID-19 testing using saliva has been proposed as an efficient, inexpensive, and non-invasive method to allow larger-scale testing, especially in a screening setting. In this study, we aimed to evaluate pooled RT-qPCR saliva testing and to compare the results with individual tests. Employees of Philips Japan, Ltd. were recruited to participate in COVID-19 screening from October to December 2020. Asymptomatic individuals (n = 824) submitted self-collected saliva samples. Samples were tested for the presence of SARS-CoV-2 by RT-qPCR in both 10-sample pools and individual tests. We also surveyed participants regarding their thoughts and behaviors after the PCR screening project. Two of the 824 individuals were positive by RT-qPCR. In the pooled testing, one of these two had no measurable Ct value, but showed an amplification trend at the end of the PCR cycle. Both positive individuals developed cold-like symptoms, but neither required hospitalization. Of the 824 participants, 471 responded to our online questionnaire. Overall, while respondents agreed that PCR screening should be performed regularly, the majority were willing to undergo PCR testing only when it was provided for free or at low cost. In conclusion, pooled testing of saliva samples can support frequent large-scale screening that is rapid, efficient, and inexpensive.

  • Reprogramming of bivalent chromatin states in NRAS mutant melanoma suggests PRC2 inhibition as a therapeutic strategy

    Terranova C.J., Tang M., Maitituoheti M., Raman A.T., Ghosh A.K., Schulz J., Amin S.B., Orouji E., Tomczak K., Sarkar S., Oba J., Creasy C., Wu C.J., Khan S., Lazcano R., Wani K., Singh A., Barrodia P., Zhao D., Chen K., Haydu L.E., Wang W.L., Lazar A.J., Woodman S.E., Bernatchez C., Rai K.

    Cell Reports (Cell Reports)  36 ( 3 ) 109410 2021年07月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  2211-1247

     概要を見る

    The dynamic evolution of chromatin state patterns during metastasis, their relationship with bona fide genetic drivers, and their therapeutic vulnerabilities are not completely understood. Combinatorial chromatin state profiling of 46 melanoma samples reveals an association of NRAS mutants with bivalent histone H3 lysine 27 trimethylation (H3K27me3) and Polycomb repressive complex 2. Reprogramming of bivalent domains during metastasis occurs on master transcription factors of a mesenchymal phenotype, including ZEB1, TWIST1, and CDH1. Resolution of bivalency using pharmacological inhibition of EZH2 decreases invasive capacity of melanoma cells and markedly reduces tumor burden in vivo, specifically in NRAS mutants. Coincident with bivalent reprogramming, the increased expression of pro-metastatic and melanocyte-specific cell-identity genes is associated with exceptionally wide H3K4me3 domains, suggesting a role for this epigenetic element. Overall, we demonstrate that reprogramming of bivalent and broad domains represents key epigenetic alterations in metastatic melanoma and that EZH2 plus MEK inhibition may provide a promising therapeutic strategy for NRAS mutant melanoma patients.

  • The genetic and epigenetic basis of distinct melanoma types

    Oba J., Woodman S.E.

    Journal of Dermatology 48 ( 7 ) 925 - 939 2021年07月

    研究論文(学術雑誌), 共著, 筆頭著者, 査読有り,  ISSN  03852407

     概要を見る

    Melanoma represents the deadliest skin cancer. Recent therapeutic developments, including targeted and immune therapies have revolutionized clinical management and improved patient outcome. This progress was achieved by rigorous molecular and functional studies followed by robust clinical trials. The identification of key genomic alterations and gene expression profiles have propelled the understanding of distinct characteristics within melanoma subtypes. The aim of this review is to summarize and highlight the main genetic and epigenetic findings of melanomas and highlight their pathological and therapeutic importance.

  • RT-PCR screening tests for SARS-CoV-2 with saliva samples in asymptomatic people: Strategy to maintain social and economic activities while reducing the risk of spreading the virus

    Oba J., Taniguchi H., Sato M., Takamatsu R., Morikawa S., Nakagawa T., Takaishi H., Saya H., Matsuo K., Nishihara H.

    Keio Journal of Medicine 70 ( 2 ) 35 - 43 2021年06月

    研究論文(学術雑誌), 共著, 筆頭著者, 査読有り,  ISSN  00229717

     概要を見る

    The year 2020 will be remembered for the coronavirus disease 2019 (COVID-19) pandemic, which continues to affect the whole world. Early and accurate identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is fundamental to combat the disease. Among the current diagnostic tests, real-time reverse transcriptase–polymerase chain reaction (RT-qPCR) is the most reliable and frequently used method. Herein, we discuss the interpretation of RT-qPCR results relative to viral infectivity. Although nasopharyngeal swab samples are often used for RT-qPCR testing, they require collection by trained medical staff. Saliva samples are emerging as an inexpensive and efficient alternative for large-scale screening. Pooled-sample testing of saliva has been applied for mass screening of SARS-CoV-2 infection. Current policies recommend isolating people with borderline cycle threshold (Ct) values (35<Ct <40), despite these Ct values indicating minimal infection risk. We propose the new concept of a “social cut-off” Ct value and risk stratification based on the correlation of Ct with infectivity. We also describe the experience of RT-qPCR screening of saliva samples at our institution. It is important to implement a scientific approach to minimize viral transmission while allowing economic and social activities to continue.

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競争的研究費の研究課題 【 表示 / 非表示

  • パンデミック状況下における可塑的対応を可能とする検査システムモデルの構築

    2021年04月
    -
    2024年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 大場 純奈, 基盤研究(C), 補助金,  研究代表者