若山 正隆 (ワカヤマ マサタカ)

WAKAYAMA Masataka

写真a

所属(所属キャンパス)

政策・メディア研究科 先端生命科学研究所 (湘南藤沢)

職名

特任講師(有期)

外部リンク

学位 【 表示 / 非表示

  • 博士(学術), 埼玉大学, 課程, 2006年03月

 

論文 【 表示 / 非表示

  • Effect of the light and dark conditions on flower opening time between cultivated rice (Oryza sativa L.) and a near-isogenic early-morning flowering line

    Tsutomu Ishimaru, Kazuhiro Sasaki, Ikuo Nozaki, Masanori Ichihashi, Hiroaki Shimizu, Masataka Wakayama, Hideyuki Hirabayashi

    AoB PLANTS (Oxford University Press ({OUP}))  2021年07月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  2041-2851

     概要を見る

    <jats:title>Abstract</jats:title>
    <jats:p>Flower opening time (FOT) is affected by genetic and environmental factors, but little is known about the effect of light and dark conditions on FOT in cereal crops. FOT of an indica rice cultivar, IR64, and its near-isogenic line carrying a QTL for an early-morning flowering trait (IR64+qEMF3) were investigated in a natural light and temperature-controlled small greenhouse by exposing either the panicle or stem or both plant organs to different light and dark conditions. FOT did not change in either genotype when panicles were exposed to light. A large difference in FOT was found between genotypes when panicles were exposed to dark conditions; no flower opening was observed in IR64, whereas flower opening was delayed but observed in IR64+qEMF3. These results suggest that the panicle is the organ that perceives light for flower opening in both genotypes, whereas the light requirement to reach flower opening was quite different between genotypes. Flower opening of IR64 occurred concomitantly with elongation of anther filament in the light after the dark treatment stopped, whereas approximately half of flowering of IR64+qEMF3 resulted in apparent cleistogamy even during dark treatment. An extended duration of the dark treatment until 1730H (30-50 min before sunset) made FOT of IR64 spikelets on the next day shifted to a time as early as that of IR64+qEMF3, with significant advancement of FOT compared to the control IR64 spikelets. Our results indicated that different flowering responses to light and dark conditions exist between IR64 and IR64+qEMF3. These findings provide clues for understanding the unique genetic controls of flowering in an early-morning flowering line in rice. This study also showed evidence that artificial light environments can shift the FOT of IR64 to that of IR64+qEMF3.</jats:p>

  • Effects of feed crops and boiling on chicken egg yolk and white determined by a metabolome analysis

    Tatsuki Ogura, Masataka Wakayama, Yujin Ashino, Rie Kadowaki, Miyu Sato, Tomoyoshi Soga, Masaru Tomita

    Food Chemistry (Elsevier BV)  327   127077 - 127077 2020年10月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  0308-8146

     概要を見る

    © 2020 Elsevier Ltd Compositional analyses of eggs have primarily focused on nutritional components, including large molecules, such as proteins. However, few reports have investigated the effects of heating and hen feed crops on taste components, such as free amino acids and sugars. Herein, water-soluble metabolites in raw and boiled eggs produced from chickens raised with corn- or rice-fed were analyzed. Each egg was separated into yolk and white, and freeze-dried samples were analyzed by CE-MS and LC-MS. Abundant metabolites included amino acids in yolks and sugars in whites. Compared to corn-fed, rice-fed resulted in three times higher betaine and uridine monophosphate concentrations in yolks and whites, respectively. Boiled whites contained more than four times higher concentrations of amino acids and fructose than raw whites. Metabolites in yolks exhibited minimal changes after boiling. Our findings support the use of water-soluble metabolomics to evaluate the effects of heating and feed crops on taste components.

  • Effect of blanching on the concentration of metabolites in two parts of Undaria pinnatifida, Wakame (leaf) and Mekabu (sporophyll)

    Shahlizah Sahul Hamid, Masataka Wakayama, Yujin Ashino, Rie Kadowaki, Tomoyoshi Soga, Masaru Tomita

    Algal Research (Algal Research)  47   101829 2020年05月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  2211-9264

     概要を見る

    © 2020 Undaria pinnatifida is a seaweed native to Japan, where it is cultivated and sold commercially as food. Fresh seaweed commonly blanches before consumption. However, the effects of blanching on metabolites present in different parts of U. pinnatifida, namely, Wakame (leaf) and Mekabu (sporophyll), are not well understood. Here, we investigated changes in metabolites in these two seaweed parts after blanching for different periods. The samples were freeze-dried and extracted using methanol-water, and metabolite concentration was quantitated using liquid chromatography mass spectrometry and capillary electrophoresis mass spectrometry. The concentration of most metabolites was the highest in raw samples, decreasing drastically within 20 s of blanching in Wakame and decreasing gradually in Mekabu. In contrast, the concentration of some fatty acids, such as octanoate, increased in both parts. During blanching, seaweed color changed from brown to green, and blanching did not significantly affect amino acid or the sugar composition. Finally, a relationship was found between metabolite and blanching-related characteristics, such as color and drying percentage.

  • 山形県庄内産メロンのメタボローム解析と果芯エキス開発

    菅原 哲也, 若山 正隆, 服部 愛衣

    日本食品科学工学会誌 67 ( 1 ) 24 - 34 2020年01月

    研究論文(学術雑誌), 共著, 査読有り

  • Metabolome profiling of various seaweed species discriminates between brown, red, and green algae

    Hamid S., Wakayama M., Ichihara K., Sakurai K., Ashino Y., Kadowaki R., Soga T., Tomita M.

    Planta (Planta)  249 ( 6 ) 1921 - 1947 2019年

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  00320935

     概要を見る

    © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Main Conclusion: Among seaweed groups, brown algae had characteristically high concentrations of mannitol, and green algae were characterised by fructose. In red algae, metabolite profiles of individual species should be evaluated. Seaweeds are metabolically different from terrestrial plants. However, general metabolite profiles of the three major seaweed groups, the brown, red, and green algae, and the effect of various extraction methods on metabolite profiling results have not been comprehensively explored. In this study, we evaluated the water-soluble metabolites in four brown, five red, and two green algae species collected from two sites in northern Japan, located in the Sea of Japan and the Pacific Ocean. Freeze-dried seaweed samples were processed by methanol–water extraction with or without chloroform and analysed by capillary electrophoresis- and liquid chromatography-mass spectrometry for metabolite characterisation. The metabolite concentration profiles showed distinctive characteristic depends on species and taxonomic groups, whereas the extraction methods did not have a significant effect. Taxonomic differences between the various seaweed metabolite profiles were well defined using only sugar metabolites but no other major compound types. Mannitol was the main sugar metabolites in brown algae, whereas fructose, sucrose, and glucose were found at high concentrations in green algae. In red algae, individual species had some characteristic metabolites, such as sorbitol in Pyropia pseudolinearis and panose in Dasya sessilis. The metabolite profiles generated in this study will be a resource and provide guidance for nutraceutical research studies because the information about metabolites in seaweeds is still very limited compared to that of terrestrial plants.

全件表示 >>

KOARA(リポジトリ)収録論文等 【 表示 / 非表示

  • 食と健康の科学

    若山, 正隆

    Keio SFC journal (慶應義塾大学湘南藤沢学会)  15 ( 1 ) 178 - 188 2015年

    ISSN  13472828

研究発表 【 表示 / 非表示

  • キャピラリー電気泳動-質量分析法を用いて多様な食品・農産物をいかに安定的に分析するのか

    若山正隆

    電気泳動(Web), 

    2020年

    その他

  • 高温登熟耐性の評価を目指したイネ幼植物体のメタボローム解析

    佐々木一嘉, 佐々木一嘉, 若山正隆, 曽我朋義, 冨田勝

    育種学研究, 

    2020年

    その他

  • 光環境によるイネの開花時刻の変化

    石丸努, 佐々木和浩, 野崎育雄, 市橋正則, 清水宏彰, 若山正隆, 平林秀介

    日本作物学会講演会要旨集, 

    2020年

    その他

  • CE-MSを用いた定量的なグルタミン分解速度の解析

    小倉立己, 若山正隆, 曽我朋義, 冨田勝

    質量分析総合討論会講演要旨集, 

    2019年

    その他

  • 鶏卵の水溶性成分の卵黄,卵白における加熱評価

    小倉立己, 若山正隆, 芦野祐尋, 曽我朋義, 冨田勝

    日本食品科学工学会大会講演集, 

    2019年

    その他

全件表示 >>

競争的研究費の研究課題 【 表示 / 非表示

  • 不均一水分環境条件下における作物の器官間代謝変動

    2020年04月
    -
    2023年03月

    名古屋大学, 仲田 麻奈、若山 正隆, 基盤研究(C), 未設定

     研究概要を見る

    作物の主要な生産性規定要因である水ストレスを、時間的・空間的水分不均一性に着目し、水ストレス適応性向上に関連する代謝物の同定ならびに遺伝制御について明らかにすることを目的とする。作物の器官間とくに根系の構成根別の代謝物の変動とそれらの遺伝変異ならびに発現遺伝子の網羅的な解析を行う。あわせて低コストでハイスループットなフェノタイピング手法の確立を目指す。本研究の成果より、作物の水ストレス適応性指標となるバイオマーカーの探索やフェノタイピング技術の開発に結びつく、今後の根系育種を発展させる有用な知見の提供に繋がることが期待される。

  • イネの開花時刻に関わる分子制御機構の解明

    2018年04月
    -
    2021年03月

    国立研究開発法人農業・食品産業技術総合研究機構, 石丸 努、若山 正隆, 石崎 琢磨, 佐々木 和浩, 基盤研究(B), 未設定

 

担当授業科目 【 表示 / 非表示

  • 生命科学実験の基礎

    2022年度

  • 生命科学実験の基礎

    2021年度

  • 生命科学実験の基礎

    2019年度