Graduate School of Media and Governance institute for Advanced Biosciences (Shonan Fujisawa)


Project Senior Assistant Professor (Non-tenured)/Project Assistant Professor (Non-tenured)/Project Lecturer (Non-tenured)

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Academic Degrees 【 Display / hide

  • Ph.D., Saitama University, Coursework, 2006.03


Papers 【 Display / hide

  • Effect of the light and dark conditions on flower opening time between cultivated rice (Oryza sativa L.) and a near-isogenic early-morning flowering line

    Tsutomu Ishimaru, Kazuhiro Sasaki, Ikuo Nozaki, Masanori Ichihashi, Hiroaki Shimizu, Masataka Wakayama, Hideyuki Hirabayashi

    AoB PLANTS (Oxford University Press ({OUP}))   2021.07

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  2041-2851

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    <jats:p>Flower opening time (FOT) is affected by genetic and environmental factors, but little is known about the effect of light and dark conditions on FOT in cereal crops. FOT of an indica rice cultivar, IR64, and its near-isogenic line carrying a QTL for an early-morning flowering trait (IR64+qEMF3) were investigated in a natural light and temperature-controlled small greenhouse by exposing either the panicle or stem or both plant organs to different light and dark conditions. FOT did not change in either genotype when panicles were exposed to light. A large difference in FOT was found between genotypes when panicles were exposed to dark conditions; no flower opening was observed in IR64, whereas flower opening was delayed but observed in IR64+qEMF3. These results suggest that the panicle is the organ that perceives light for flower opening in both genotypes, whereas the light requirement to reach flower opening was quite different between genotypes. Flower opening of IR64 occurred concomitantly with elongation of anther filament in the light after the dark treatment stopped, whereas approximately half of flowering of IR64+qEMF3 resulted in apparent cleistogamy even during dark treatment. An extended duration of the dark treatment until 1730H (30-50 min before sunset) made FOT of IR64 spikelets on the next day shifted to a time as early as that of IR64+qEMF3, with significant advancement of FOT compared to the control IR64 spikelets. Our results indicated that different flowering responses to light and dark conditions exist between IR64 and IR64+qEMF3. These findings provide clues for understanding the unique genetic controls of flowering in an early-morning flowering line in rice. This study also showed evidence that artificial light environments can shift the FOT of IR64 to that of IR64+qEMF3.</jats:p>

  • Effects of feed crops and boiling on chicken egg yolk and white determined by a metabolome analysis

    Tatsuki Ogura, Masataka Wakayama, Yujin Ashino, Rie Kadowaki, Miyu Sato, Tomoyoshi Soga, Masaru Tomita

    Food Chemistry (Elsevier BV)  327   127077 - 127077 2020.10

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  0308-8146

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    © 2020 Elsevier Ltd Compositional analyses of eggs have primarily focused on nutritional components, including large molecules, such as proteins. However, few reports have investigated the effects of heating and hen feed crops on taste components, such as free amino acids and sugars. Herein, water-soluble metabolites in raw and boiled eggs produced from chickens raised with corn- or rice-fed were analyzed. Each egg was separated into yolk and white, and freeze-dried samples were analyzed by CE-MS and LC-MS. Abundant metabolites included amino acids in yolks and sugars in whites. Compared to corn-fed, rice-fed resulted in three times higher betaine and uridine monophosphate concentrations in yolks and whites, respectively. Boiled whites contained more than four times higher concentrations of amino acids and fructose than raw whites. Metabolites in yolks exhibited minimal changes after boiling. Our findings support the use of water-soluble metabolomics to evaluate the effects of heating and feed crops on taste components.

  • Effect of blanching on the concentration of metabolites in two parts of Undaria pinnatifida, Wakame (leaf) and Mekabu (sporophyll)

    Shahlizah Sahul Hamid, Masataka Wakayama, Yujin Ashino, Rie Kadowaki, Tomoyoshi Soga, Masaru Tomita

    Algal Research (Algal Research)  47   101829 2020.05

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  2211-9264

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    © 2020 Undaria pinnatifida is a seaweed native to Japan, where it is cultivated and sold commercially as food. Fresh seaweed commonly blanches before consumption. However, the effects of blanching on metabolites present in different parts of U. pinnatifida, namely, Wakame (leaf) and Mekabu (sporophyll), are not well understood. Here, we investigated changes in metabolites in these two seaweed parts after blanching for different periods. The samples were freeze-dried and extracted using methanol-water, and metabolite concentration was quantitated using liquid chromatography mass spectrometry and capillary electrophoresis mass spectrometry. The concentration of most metabolites was the highest in raw samples, decreasing drastically within 20 s of blanching in Wakame and decreasing gradually in Mekabu. In contrast, the concentration of some fatty acids, such as octanoate, increased in both parts. During blanching, seaweed color changed from brown to green, and blanching did not significantly affect amino acid or the sugar composition. Finally, a relationship was found between metabolite and blanching-related characteristics, such as color and drying percentage.

  • Metabolomic Profiling of Melon (Cucumis melo L.) Cultivated in Shonai, Yamagata Prefecture and Development of Effective Utilization of Residues from Fruit Processing

    SUGAWARA, T, WAKAYAMA, M, Hattori, A

    日本食品科学工学会誌 67 ( 1 ) 24 - 34 2020.01

    Research paper (scientific journal), Joint Work, Accepted

  • Metabolome profiling of various seaweed species discriminates between brown, red, and green algae

    Hamid SS, Wakayama M, Ichihara K, Sakurai K, Ashino Y, Kadowaki R, Soga T, Tomita M

    Planta (Planta)  249 ( 6 ) 1921 - 1947 2019

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  00320935

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    © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Main Conclusion: Among seaweed groups, brown algae had characteristically high concentrations of mannitol, and green algae were characterised by fructose. In red algae, metabolite profiles of individual species should be evaluated. Seaweeds are metabolically different from terrestrial plants. However, general metabolite profiles of the three major seaweed groups, the brown, red, and green algae, and the effect of various extraction methods on metabolite profiling results have not been comprehensively explored. In this study, we evaluated the water-soluble metabolites in four brown, five red, and two green algae species collected from two sites in northern Japan, located in the Sea of Japan and the Pacific Ocean. Freeze-dried seaweed samples were processed by methanol–water extraction with or without chloroform and analysed by capillary electrophoresis- and liquid chromatography-mass spectrometry for metabolite characterisation. The metabolite concentration profiles showed distinctive characteristic depends on species and taxonomic groups, whereas the extraction methods did not have a significant effect. Taxonomic differences between the various seaweed metabolite profiles were well defined using only sugar metabolites but no other major compound types. Mannitol was the main sugar metabolites in brown algae, whereas fructose, sucrose, and glucose were found at high concentrations in green algae. In red algae, individual species had some characteristic metabolites, such as sorbitol in Pyropia pseudolinearis and panose in Dasya sessilis. The metabolite profiles generated in this study will be a resource and provide guidance for nutraceutical research studies because the information about metabolites in seaweeds is still very limited compared to that of terrestrial plants.

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Papers, etc., Registered in KOARA 【 Display / hide

  • Food and health sciences

    Wakayama, Masataka

    Keio SFC journal (慶應義塾大学湘南藤沢学会)  15 ( 1 ) 178 - 188 2015

    ISSN  13472828

Presentations 【 Display / hide

  • キャピラリー電気泳動-質量分析法を用いて多様な食品・農産物をいかに安定的に分析するのか





  • Metabolome analysis of rice seedlings for high temperature resistance evaluation

    佐々木一嘉, 佐々木一嘉, 若山正隆, 曽我朋義, 冨田勝




  • Changes in flower opening time by artificial light and dark conditions in rice

    石丸努, 佐々木和浩, 野崎育雄, 市橋正則, 清水宏彰, 若山正隆, 平林秀介




  • CE-MSを用いた定量的なグルタミン分解速度の解析

    小倉立己, 若山正隆, 曽我朋義, 冨田勝




  • 鶏卵の水溶性成分の卵黄,卵白における加熱評価

    小倉立己, 若山正隆, 芦野祐尋, 曽我朋義, 冨田勝




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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Metabolite dynamics among different organs in crop under heterogeneous water environment


    Nagoya University, Grant-in-Aid for Scientific Research (C), No Setting

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  • イネの開花時刻に関わる分子制御機構の解明


    National Agriculture and Food Research Organization, Grant-in-Aid for Scientific Research (B), No Setting


Courses Taught 【 Display / hide