Papers - Fujiwara, Kei

Introducing Micrometer-Sized Artificial Objects into Live Cells: A Method for Cell–Giant Unilamellar Vesicle Electrofusion

Akira C Saito, Toshihiko Ogura, Kei Fujiwara, Satoshi Murata, Shin-ichiro M Nomura

PloS one 9   e106853 2014.09

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Generation of Giant Unilamellar Liposomes Containing Biomacromolecules at Physiological Intracellular Concentrations using Hypertonic Conditions.

Yanagisawa, Miho, Fujiwara, Kei

ACS Synthetic Biology 3 ( 12 ) 870 - 874 2014.02

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

DNAナノテクノロジーのためのアウトリーチツールの開発と評価

吉澤 慧, 藤原 慶, 野村 M.慎一郎, 村田 智

形の科学会誌 (形の科学会)  29 ( 2 )  2014

Research paper (scientific journal), Joint Work

The rapid synthesis of cell-sized liposomes by centrifuge-based microfluidic device

Morita Masamune, Onoe Hiroaki, Yanagisawa Miho, Fujiwara Kei, Saito Hirohide, Takinoue Masahiro.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (Chemical and Biological Microsystems Society)     336 - 338 2014

Research paper (international conference proceedings), Joint Work

　View Summary

<p>We developed a simple production method of monodisperse cell-sized liposomes by centrifuge-based droplet shooting device (CDSD)&lt;sup&gt;1&lt;/sup&gt;. This device does not require specialized equipment compared to other microfluidic techniques, and furthermore, this device needs a very small volume of encapsulated solutions (0.5-5 μL). We hope that this method will allow to make cell-sized liposomes for a given biological application.</p>

細胞内濃厚環境に近づいた無細胞発現系

FUJIWARA Kei, Nomura M. Shin-ichiro

生物物理 53 ( 5 ) 262 - 263 2013.09

Joint Work, Accepted

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Molecular robotics approach for constructing an artificial cell model

Shin-Ichiro M Nomura, Yusuke Sato, Kei Fujiwara

Advances in Artificial Life, ECAL 12   488 - 491 2013.09

Research paper (international conference proceedings), Joint Work, Accepted

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Construction of a remote-controlled supramolecular micro-crawler

Daiki Komatsu, Kei Fujiwara, Shin-Ichiro M Nomura

Advances in Artificial Life, ECAL 12   208 - 209 2013.09

Research paper (international conference proceedings), Joint Work, Accepted

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Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

Fujiwara, Kei, Katayama, Tsutomu, Nomura, Shin-ichiro M.

NUCLEIC ACIDS RESEARCH 41 ( 14 ) 7176 - 7183 2013.08

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0305-1048

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Physicochemical Analysis from Real-Time Imaging of Liposome Tubulation Reveals the Characteristics of Individual F-BAR Domain Proteins

Tanaka-Takiguchi, Yohko, Itoh, Toshiki, Tsujita, Kazuya, Yamada, Shunsuke, Yanagisawa, Miho, Fujiwara, Kei, Yamamoto, Akihisa, Ichikawa, Masatoshi, Takiguchi, Kingo

LANGMUIR 29 ( 1 ) 328 - 336 2013.01

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0743-7463

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Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells

Fujiwara, Kei, Nomura, Shin-ichiro M.

PLOS ONE 8 ( 1 ) 1552 - 1564 2013.01

Research paper (scientific journal), Joint Work, Accepted,  ISSN  1932-6203

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A bacterial salt sensor created by multiplying phenotypes of GroE-depleted Escherichia coli

Fujiwara, Kei, Aoi, Keita B., Nomura, Shin-ichiro M.

ANALYTICAL METHODS 5 ( 21 ) 5918 - 5922 2013

Research paper (scientific journal), Joint Work, Accepted,  ISSN  1759-9660

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Mechanism of methionine synthase overexpression in chaperonin-depleted Escherichia coli

Fujiwara, Kei, Taguchi, Hideki

MICROBIOLOGY-SGM 158   917 - 924 2012.04

Research paper (scientific journal), Joint Work, Accepted,  ISSN  1350-0872

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Cell-Sized confinement in microspheres accelerates the reaction of gene expression

Kato, Ayako, Yanagisawa, Miho, Sato, Yuko T., Fujiwara, Kei, Yoshikawa, Kenichi

SCIENTIFIC REPORTS 2   5918 - 5922 2012.02

Research paper (scientific journal), Joint Work, Accepted,  ISSN  2045-2322

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A systematic survey of in vivo obligate chaperonin-dependent substrates

Fujiwara, Kei, Ishihama, Yasushi, Nakahigashi, Kenji, Soga, Tomoyoshi, Taguchi, Hideki

EMBO JOURNAL 29 ( 9 ) 1552 - 1564 2010.05

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0261-4189

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Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE

Fujiwara, Kei, Taguchi, Hideki

JOURNAL OF BACTERIOLOGY 189 ( 16 ) 5860 - 5866 2007.08

Research paper (scientific journal), Accepted,  ISSN  0021-9193

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Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus

Fujiwara, Kei, Tsubouchi, Taishi, Kuzuyama, Tomohisa, Nishiyama, Makoto

MICROBIOLOGY-SGM 152   3585 - 3594 2006.12

Research paper (scientific journal), Joint Work, Accepted,  ISSN  1350-0872