Details of a Researcher - Sato, Toshinori

Papers - Sato, Toshinori

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Effects of the Magnetic Orientation of M13 Bacteriophage on Phage Display Selection

Wang S., Uchida N., Ueno K., Matsubara T., Sato T., Aida T., Ishida Y.

Chemistry - A European Journal (Chemistry - A European Journal) 29 ( 63 ) 2023.11

ISSN 09476539

Although phage display selection using a library of M13 bacteriophage has become a powerful tool for finding peptides that bind to target materials on demand, a remaining concern of this method is the interference by the M13 main body, which is a huge filament >103 times larger than the displayed peptide, and therefore would nonspecifically adhere to the target or sterically inhibit the binding of the displayed peptide. Meanwhile, filamentous phages are known to be orientable by an external magnetic field. If M13 filaments are magnetically oriented during the library selection, their angular arrangement relative to the target surface would be changed, being expected to control the interference by the M13 main body. This study reports that the magnetic orientation of M13 filaments vertical to the target surface significantly affects the selection. When the target surface was affinitive to the M13 main body, this orientation notably suppressed the nonspecific adhesion. Furthermore, when the target surface was less affinitive to the M13 main body and intrinsically free from the nonspecific adhesion, this orientation drastically changed the population of M13 clones obtained through library selection. The method of using no chemicals but only a physical stimulus is simple, clean, and expected to expand the scope of phage display selection.

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The sialyl-Tn antigen synthase genes regulates migration–proliferation dichotomy in prostate cancer cells under hypoxia

Yamamoto D., Hongo H., Kosaka T., Aoki N., Oya M., Sato T.

Glycoconjugate Journal (Glycoconjugate Journal) 40 ( 2 ) 199 - 212 2023.04

ISSN 02820080

A low-oxygen (hypoxia) tumor microenvironment can facilitate chemotherapy and radiation therapy resistance in tumors and is associated with a poor prognosis. Hypoxia also affects PCa (prostate cancer) phenotype transformation and causes therapeutic resistance. Although O-glycans are known to be involved in the malignancy of various cancers under hypoxia, the expression and function of O-glycans in PCa are not well understood. In this study, the saccharide primer method was employed to analyze O-glycan expression in PCa cells. Results showed that the expression of sTn antigens was increased in PCa cells under hypoxia. Furthermore, it was found that ST6GalNAc1, the sTn antigen synthase gene, was involved in the migration–proliferation dichotomy and drug resistance in PCa cells under hypoxia. The results of this study will contribute to the development of novel diagnostic markers and drug targets for PCa under hypoxia.

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Endocytosis-Like Vesicle Fission Mediated by a Membrane-Expanding Molecular Machine Enables Virus Encapsulation for In Vivo Delivery

Uchida N., Ryu Y., Takagi Y., Yoshizawa K., Suzuki K., Anraku Y., Ajioka I., Shimokawa N., Takagi M., Hoshino N., Akutagawa T., Matsubara T., Sato T., Higuchi Y., Ito H., Morita M., Muraoka T.

Journal of the American Chemical Society (Journal of the American Chemical Society) 145 ( 11 ) 6210 - 6220 2023.03

ISSN 00027863

Biological membranes are functionalized by membrane-associated protein machinery. Membrane-associated transport processes, such as endocytosis, represent a fundamental and universal function mediated by membrane-deforming protein machines, by which small biomolecules and even micrometer-size substances can be transported via encapsulation into membrane vesicles. Although synthetic molecules that induce dynamic membrane deformation have been reported, a molecular approach enabling membrane transport in which membrane deformation is coupled with substance binding and transport remains critically lacking. Here, we developed an amphiphilic molecular machine containing a photoresponsive diazocine core (AzoMEx) that localizes in a phospholipid membrane. Upon photoirradiation, AzoMEx expands the liposomal membrane to bias vesicles toward outside-in fission in the membrane deformation process. Cargo components, including micrometer-size M13 bacteriophages that interact with AzoMEx, are efficiently incorporated into the vesicles through the outside-in fission. Encapsulated M13 bacteriophages are transiently protected from the external environment and therefore retain biological activity during distribution throughout the body via the blood following administration. This research developed a molecular approach using synthetic molecular machinery for membrane functionalization to transport micrometer-size substances and objects via vesicle encapsulation. The molecular design demonstrated in this study to expand the membrane for deformation and binding to a cargo component can lead to the development of drug delivery materials and chemical tools for controlling cellular activities.

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Dihydromaniwamycin E, a Heat-Shock Metabolite from Thermotolerant Streptomyces sp. JA74, Exhibiting Antiviral Activity against Influenza and SARS-CoV-2 Viruses

Saito S., Funayama K., Kato W., Okuda M., Kawamoto M., Matsubara T., Sato T., Sato A., Otsuguro S., Sasaki M., Orba Y., Sawa H., Maenaka K., Shindo K., Imoto M., Arai M.A.

Journal of Natural Products (Journal of Natural Products) 85 ( 11 ) 2583 - 2591 2022.11

ISSN 01633864

Dihydromaniwamycin E (1), a new maniwamycin derivative featuring an azoxy moiety, has been isolated from the culture extract of thermotolerant Streptomyces sp. JA74 along with the known analogue maniwamycin E (2). Compound 1 is produced only by cultivation of strain JA74 at 45 °C, and this type of compound has been previously designated a "heat shock metabolite (HSM)" by our research group. Compound 2 is detected as a production-enhanced metabolite at high temperature. Structures of 1 and 2 are elucidated by NMR and MS spectroscopic analyses. The absolute structure of 1 is determined after the total synthesis of four stereoisomers. Though the absolute structure of 2 has been proposed to be the same as the structure of maniwamycin D, the NMR and the optical rotation value of 2 are in agreement with those of maniwamycin E. Therefore, this study proposes a structural revision of maniwamycins D and E. Compounds 1 and 2 show inhibitory activity against the influenza (H1N1) virus infection of MDCK cells, demonstrating IC50values of 25.7 and 63.2 μM, respectively. Notably, 1 and 2 display antiviral activity against SARS-CoV-2, the causative agent of COVID-19, when used to infect 293TA and VeroE6T cells, with 1 and 2 showing IC50values (for infection of 293TA cells) of 19.7 and 9.7 μM, respectively. The two compounds do not exhibit cytotoxicity in these cell lines at those IC50concentrations.

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Effective Cell Transfection in An Ultrasonically Levitated Droplet for Sustainable Technology

Arai T., Sato T., Matsubara T.

Advanced Science (Advanced Science) 9 ( 30 ) 2022.10

The levitation methodology, which enables us to operate a contactless reaction without a container, is likely to be a revolutionary technology in the fields of chemistry and biology to reduce the plastic waste in life science laboratories. Here, the authors show that plasmid DNA can be effectively transfected into animal cells in a floating droplet of culture medium levitated using ultrasonic standing waves. The data indicate that there is no significant damage to the plasmid and cells during the levitating transfection time, and the transgene expression efficiency and cellular uptake in the droplet are significantly higher than those in the conventional tube, with and without shaking. These results suggest the consolidation of the endocytic uptake pathway into macropinocytosis, indicating that ultrasonic levitation induced a change in cell characteristics. This study suggests that transfection methodology using ultrasonic levitation has the potential to advance the current experimental procedures in the field of cell engineering, in addition to presenting a revolutionary containerless reactor for sustainable technology.

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Functional analysis of GCNT3 for cell migration and EMT of castration-resistant prostate cancer cells

Yamamoto D., Sasaki K., Kosaka T., Oya M., Sato T.

Glycobiology (Glycobiology) 32 ( 10 ) 897 - 908 2022.09

Castration-resistant prostate cancer (CRPC) is a malignant tumor that is resistant to androgen deprivation therapy. Treatments for CRPC are limited, and no diagnostic markers are currently available. O-glycans are known to play an important role in cell proliferation, migration, invasion, and metastasis of cancer cells. However, the differences in the O-glycan expression profiles for normal prostate cancer (PCa) cells compared with CRPC cells have not yet been investigated. In this study, the saccharide primer method was employed to analyze the O-glycans expressed in CRPC cells. Expression levels of core 4-type O-glycans were significantly increased in CRPC cells. Furthermore, the expression level of N-Acetylglucosaminyltransferase 3 (GCNT3), a core 4-type O-glycan synthase gene, was increased in CRPC cells. The expression of core 4-type O-glycans and GCNT3 was presumed to be regulated by androgen deprivation. GCNT3 knockdown induced cell migration and epithelial-mesenchymal transition. These observations elucidate the mechanism of acquisition of castration resistance in PCa and offer new possibilities for the development of diagnostic markers and therapeutic targets in the treatment of PCa.

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Ganglioside Nanocluster-Targeting Peptidyl Inhibitor Prevents Amyloid β Fibril Formation on the Neuronal Membrane

Matsubara T., Nakai M., Nishihara M., Miyamoto E., Sato T.

ACS Chemical Neuroscience (ACS Chemical Neuroscience) 13 ( 13 ) 1868 - 1876 2022.07

Neurotoxicity caused by peptide and protein aggregates is associated with the onset of neurodegenerative diseases. Accumulation of the amyloid β protein (Aβ) induced by neuronal ganglioside-enriched nanodomains (nanoclusters) in the presynaptic neuronal membrane, resulting in toxic oligomeric and fibrous forms, is implicated in the onset of Alzheimer's disease (AD). In the current study, we found that the ganglioside cluster-binding peptide (GCBP), a pentadecapeptide VWRLLAPPFSNRLLP that binds to ganglioside-enriched nanoclusters, inhibits the formation of Aβ assemblies with an IC50 of 12 pM and also removes Aβ fibrils deposited on the lipid membrane. Thus, in addition to inhibiting Aβ assembly formation, GCBP effectively clears toxic Aβ assemblies as well, thereby suppressing neuronal cellular damage and death induced by such assemblies. These results indicate that ganglioside cluster-binding molecules may act as novel Aβ-targeting drugs with a unique mechanism of action that may be utilized to ameliorate AD.

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Accelerated transgene expression of pDNA/polysaccharide complexes by solid-phase reverse transfection and analysis of the cell transfection mechanism

Arai T., Aiki Y., Sato T.

Polymer Journal (Polymer Journal) 54 ( 4 ) 603 - 613 2022.04

ISSN 00323896

Gene delivery using pDNA complexes immobilized on a substrate, termed solid-phase reverse transfection (RTF), has received increasing attention. This is because it can enhance transgene expression and localize gene delivery. However, gene carriers used in RTF are limited, and their transgene expression mechanisms are unclear. In this study, we employed pDNA/chitosan/anionic polysaccharide complexes for RTF and investigated their cell transfection mechanisms. The pDNA/chitosan/hyaluronic acid (HA) ternary complexes showed particularly higher transgene expression than other ternary complexes. Cellular uptake and intracellular trafficking pathways were analyzed using inhibitors of endocytosis and intracellular trafficking. It was revealed that pDNA/chitosan/HA complexes were taken up via macropinocytosis and released from late endosomes. Furthermore, pDNA complexes were remarkably accumulated in the nucleus in RTF compared with the conventional forward transfection (FTF) method. These results suggest that the cell transfection efficiency of the pDNA/chitosan/HA ternary complexes in RTF was enhanced by their efficient delivery to the nucleus via late endosomes.

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De Novo Design of Star-Shaped Glycoligands with Synthetic Polymer Structures toward an Influenza Hemagglutinin Inhibitor

Nagao M., Yamaguchi A., Matsubara T., Hoshino Y., Sato T., Miura Y.

Biomacromolecules (Biomacromolecules) 23 ( 3 ) 1232 - 1241 2022.03

ISSN 15257797

Synthetic polymers with well-defined structures allow the development of nanomaterials with additional functions beyond biopolymers. Herein, we demonstrate de novo design of star-shaped glycoligands to interact with hemagglutinin (HA) using well-defined synthetic polymers with the aim of developing an effective inhibitor for the influenza virus. Prior to the synthesis, the length of the star polymer chains was predicted using the Gaussian model of synthetic polymers, and the degree of polymerization required to achieve multivalent binding to three carbohydrate recognition domains (CRDs) of HA was estimated. The star polymer with the predicted degree of polymerization was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, and 6′-sialyllactose was conjugated as the glycoepitope for HA. The designed glycoligand exhibited the strongest interaction with HA as a result of multivalent binding. This finding demonstrated that the biological function of the synthetic polymer could be controlled by precisely defining the polymer structures.

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In vitro synthesis of mucin-type O-glycans using saccharide primers comprising GalNAc-Ser and GalNAc-Thr residues

Sakura R., Nagai K., Yagi Y., Takahashi Y., Ide Y., Yagi Y., Yamamoto D., Mizuno M., Sato T.

Carbohydrate Research (Carbohydrate Research) 511 2022.01

ISSN 00086215

Mucin-type O-glycosylation of serine or threonine residue in proteins is known to be one of the major post-translational modifications. In this study, two novel alkyl glycosides, Nα-lauryl-O-(2-acetamido-2-deoxy-α-D-galactopyranosyl)-L-serineamide (GalNAc-Ser-C12) and Nα-lauryl-O-(2-acetamido-2-deoxy-α-D-galactopyranosyl)-L-threonineamide (GalNAc-Thr-C12) were synthesized as saccharide primers to prime mucin-type O-glycan biosynthesis in cells. Upon incubating human gastric cancer MKN45 cells with the saccharide primers, 22 glycosylated products were obtained, and their structures were analyzed using liquid chromatography-mass spectrometry and enzyme digestion. The amounts of glycosylated products were dependent on the amino acid residues in the saccharide primers. For example, in vitro synthesis of T antigen (Galβ1-3GalNAc), fucosyl-T (Fucα1-2Galβ1-3GalNAc), and sialyl-T (NeuAcα2-3Galβ1-3GalNAc) preferred a serine residue, whereas sialyl-Tn (NeuAcα2-6GalNAc) preferred a threonine residue. Furthermore, the glycosylated products derived from GalNAc-Ser/Thr-C12 and Gal-GalNAc-Ser/Thr-C12 using cell-free synthesis showed the same amino acid selectivity as those in the cell experiments. These results indicate that glycosyltransferases involved in the biosynthesis of mucin-type O-glycans distinguish amino acid residues conjugated to GalNAc. The saccharide primers developed in this study might be useful for comparing mucin-type oligosaccharides in cells and constructing oligosaccharide libraries to study cell function.

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Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells

Matsubara T., Ogami A., Kori H., Hashizume M., Sato T.

ACS Applied Bio Materials (ACS Applied Bio Materials) 2022

The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.

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Motobamide, an Antitrypanosomal Cyclic Peptide from a Leptolyngbya sp. Marine Cyanobacterium

Takahashi H., Iwasaki A., Kurisawa N., Suzuki R., Jeelani G., Matsubara T., Sato T., Nozaki T., Suenaga K.

Journal of Natural Products (Journal of Natural Products) 84 ( 5 ) 1649 - 1655 2021.05

ISSN 01633864

Motobamide (1), a new cyclic peptide containing a C-prenylated cyclotryptophan residue, was isolated from a marine Leptolyngbya sp. cyanobacterium. Its planar structure was established by spectroscopic and MS/MS analyses. The absolute configuration was elucidated based on a combination of chemical degradations, chiral-phase HPLC analyses, spectroscopic analyses, and computational chemistry. Motobamide (1) moderately inhibited the growth of bloodstream forms of Trypanosoma brucei rhodesiense (IC50 2.3 μM). However, it exhibited a weaker cytotoxicity against normal human cells (IC50 55 μM).

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Heterogeneous Ganglioside-Enriched Nanoclusters with Different Densities in Membrane Rafts Detected by a Peptidyl Molecular Probe

Matsubara T., Iijima K., Kojima T., Hirai M., Miyamoto E., Sato T.

Langmuir (Langmuir) 37 ( 2 ) 646 - 654 2021.01

ISSN 07437463

© 2021 American Chemical Society. The specific features of the lateral distribution of gangliosides play key roles in cell-cell communications and the onset of various diseases related to the plasma membrane. We herein demonstrated that an artificial peptide identified from a phage-displayed library is available as a molecular probe for specific ganglioside nanoclustering sites in caveolae/membrane rafts on the cell surface. Atomic force microscopy studies indicated that the peptide specifically binds to the highly enriched monosialoganglioside GM1 nanodomains of reconstituted lipid bilayers composed of GM1, sphingomyelin, cholesterol, and unsaturated phospholipids. The ganglioside-containing area recognized by the peptide on the surface of PC12 cells was part of the area recognized by the cholera toxin B subunit, which has high affinity for GM1. Furthermore, the peptide bound to the cell surface after a treatment with methyl-β-cyclodextrin (MβCD), which disrupts membrane rafts by removing cholesterol. The present results indicate that there are heterogeneous ganglioside clusters with different ganglioside densities in caveolae/membrane rafts, and the peptidyl probe selectively recognizes the high-density ganglioside nanodomain that resists the MβCD treatment. This peptidyl probe will be useful for obtaining information on the lipid organization of the cell membrane and will help clarify the mechanisms by which the lateral distribution of gangliosides affects biological functions and the onset of diseases.

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Avian Influenza Virus Detection by Optimized Peptide Termination on a Boron-Doped Diamond Electrode

Matsubara T., Ujie M., Yamamoto T., Einaga Y., Daidoji T., Nakaya T., Sato T.

ACS Sensors (ACS Sensors) 5 ( 2 ) 431 - 439 2020.02

Copyright © 2020 American Chemical Society. The development of a simple detection method with high sensitivity is essential for the diagnosis and surveillance of infectious diseases. Previously, we constructed a sensitive biosensor for the detection of pathological human influenza viruses using a boron-doped diamond electrode terminated with a sialyloligosaccharide receptor-mimic peptide that could bind to hemagglutinins involved in viral infection. Circulation of influenza induced by the avian virus in humans has become a major public health concern, and methods for the detection of avian viruses are urgently needed. Here, peptide density and dendrimer generation terminated on the electrode altered the efficiency of viral binding to the electrode surface, thus significantly enhancing charge-transfer resistance measured by electrochemical impedance spectroscopy. The peptide-terminated electrodes exhibited an excellent detection limit of less than one plaque-forming unit of seasonal H1N1 and H3N2 viruses. Furthermore, the improved electrode was detectable for avian viruses isolated from H5N3, H7N1, and H9N2, showing the potential for the detection of all subtypes of influenza A virus, including new subtypes. The peptide-based electrochemical architecture provided a promising approach to biosensors for ultrasensitive detection of pathogenic microorganisms.

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Ikoamide, an Antimalarial Lipopeptide from an Okeania sp. Marine Cyanobacterium

Iwasaki K., Iwasaki A., Sumimoto S., Matsubara T., Sato T., Nozaki T., Saito-Nakano Y., Suenaga K.

Journal of Natural Products (Journal of Natural Products) 83 ( 2 ) 481 - 488 2020.02

ISSN 01633864

Copyright © 2020 American Chemical Society and American Society of Pharmacognosy. An antimalarial lipopeptide, ikoamide, was isolated from an Okeania sp. marine cyanobacterium. Its gross structure was established by spectroscopic analyses, and the absolute configuration was clarified based on a combination of chiral-phase HPLC analyses, spectroscopic analyses, and derivatization reactions. Ikoamide showed strong antimalarial activity with an IC50 value of 0.14 μM without cytotoxicity against human cancer cell lines at 10 μM.

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Detection of influenza virus by agglutination using nanoparticles conjugated with a sialic acid-mimic peptide

Matsubara T., Kubo A., Sato T.

Polymer Journal (Polymer Journal) 52 ( 2 ) 261 - 266 2020.02

ISSN 00323896

© 2019, The Society of Polymer Science, Japan. Abstract: Influenza virus (IFV) detection in the early phase of disease is critical for effective anti-influenza therapy using neuraminidase inhibitors. Sialyloligosaccharide receptors on the surface of respiratory cells are recognized by IFV hemagglutinin (HA) in the infection. Here, we show that agglutination of IFV is detected using poly(glycidyl methacrylate) (PGMA)-coated polystyrene nanoparticles conjugated with a sialic acid-mimic peptide. The azido peptide was immobilized onto the surface of the PGMA-coated nanoparticles by click chemistry. The distribution of particle size, determined by dynamic light scattering, indicated that the peptide-conjugated nanoparticles were agglutinated in the presence of HA and IFV. Nanoparticles conjugated with the receptor-mimic peptide may be a useful alternative to red blood cells in the global surveillance and clinical diagnosis of influenza.

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Synthesis of Various Glycopolymers Bearing Sialyllactose and the Effect of Their Molecular Mobility on Interaction with the Influenza Virus

Nagao M., Matsubara T., Hoshino Y., Sato T., Miura Y.

Biomacromolecules (Biomacromolecules) 20 ( 7 ) 2763 - 2769 2019.07

ISSN 15257797

© 2019 American Chemical Society. Synthetic glyco-ligands are promising candidates for effective nanomedicines against pathogens. Glycopolymers bearing sialyl-oligosaccharides interact with hemagglutinin present on the surface of influenza viruses. In designing new glycopolymers that further enhance the interaction with viruses, both static and dynamic properties of the glycopolymers should be considered. In this report, we evaluated the correlation between dynamic properties of glycopolymers and their interaction with the influenza virus. Glycopolymers with pendant sialyllactoses and various linker structures were synthesized, and their molecular mobility was determined by proton spin-spin relaxation time measurements. The molecular mobility of the glycounits increased as the length of the linker structures increased. Interestingly, glycopolymers with the medium-length linker structure exhibited the strongest interaction with the influenza virus, suggesting that optimal molecular mobility is required for maximizing multivalent interactions with the target.

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Topological Design of Star Glycopolymers for Controlling the Interaction with the Influenza Virus

Nagao M., Matsubara T., Hoshino Y., Sato T., Miura Y.

Bioconjugate Chemistry (Bioconjugate Chemistry) 30 ( 4 ) 1192 - 1198 2019.04

ISSN 10431802

© 2019 American Chemical Society. The precise design of synthetic polymer ligands using controlled polymerization techniques provides an advantage for the field of nanoscience. We report the topological design of glyco-ligands based on synthetic polymers for targeting hemagglutinin (HA, lectin on the influenza virus). To achieve precise arrangement of the glycounits toward the sugar-binding pockets of HA, triarm star glycopolymers were synthesized. The interaction of the star glycopolymers with HA was found to depend on the length of the polymer arms and was maximized when the hydrodynamic diameter of the star glycopolymer was comparable to the distance between the sugar-binding pockets of HA. Following the formula of multivalent interaction, the number of binding sites in the interaction of the glycopolymers with HA was estimated as 1.8-2.7. Considering one HA molecule has three sugar-binding pockets, these values were reasonable. The binding mode of synthetic glycopolymer-ligands toward lectins could be tuned using controlled radical polymerization techniques.

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Hoshinoamides A and B, Acyclic Lipopeptides from the Marine Cyanobacterium Caldora penicillata

Iwasaki A., Tadenuma T., Sumimoto S., Shiota I., Matsubara T., Saito-Nakano Y., Nozaki T., Sato T., Suenaga K.

Journal of Natural Products (Journal of Natural Products) 81 ( 11 ) 2545 - 2552 2018.11

ISSN 01633864

Copyright © 2018 American Chemical Society and American Society of Pharmacognosy. Hoshinoamides A (1) and B (2), new acyclic lipopeptides, were isolated from the marine cyanobacterium Caldora penicillata. Their structures were elucidated by spectroscopic analyses and degradation reactions. Hoshinoamides A (1) and B (2) did not exhibit any cytotoxicity against HeLa cells at 10 μM, but inhibited the in vitro growth of the malarial parasite Plasmodium falciparum (IC50 = 0.52 and 1.0 μM, respectively).

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Responsibility of lipid compositions for the amyloid ß assembly induced by ganglioside nanoclusters in mouse synaptosomal membranes

Matsubara T., Kojima T., Fukuda R., Iijma K., Hirai M., Yamamoto N., Yanagisawa K., Sato T.

Polymer Journal (Polymer Journal) 50 ( 8 ) 745 - 752 2018.08

ISSN 00323896

© 2018, The Society of Polymer Science, Japan. The assembly of amyloid ß protein (Aß) on ganglioside-enriched microdomains in presynaptic neuronal membranes is involved in the onset of Alzheimer’s disease. We previously found that a highly enriched ganglioside domain generated in a reconstituted lipid bilayer composed of synaptosomal lipids functioned as an Aß-sensitive ganglioside nanocluster (ASIGN) that may induce a spherical Aß assembly. In the present study, the major lipid components of a detergent-resistant membrane microdomain (DRM) fraction of synaptosomes prepared from an aged mouse brain were identified, and we demonstrate that the proportion of Aß-insensitive gangliosides such as GM3 directly affects the Aß assembly in synaptosomal membranes. Liquid chromatography coupled to mass spectrometry analyses indicated that ganglio a-series gangliosides (GM1, GM2, GM3, and GD1a) were abundant in DRM fractions. In addition, the GM3/GM1 ratio and the cholesterol content in the synaptosomal fraction differed from those in the non-synaptosomal fraction, in which GM1 and GM3 are sensitive and insensitive gangliosides, respectively. The highly enriched ganglioside nanocluster was identified by the surface topography of ternary mixed lipid bilayers composed of gangliosides, sphingomyelin, and cholesterol. Our results indicate that the composition of gangliosides is responsible for the characteristics of the nanocluster generated in the presynaptic neuronal membranes.

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Izenamides A and B, Statine-Containing Depsipeptides, and an Analogue from a Marine Cyanobacterium

Kanamori Y., Iwasaki A., Sumimoto S., Matsubara T., Sato T., Suenaga K.

Journal of Natural Products (Journal of Natural Products) 81 ( 7 ) 1673 - 1681 2018.07

ISSN 01633864

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Comparative Quantification Method for Glycosylated Products Elongated on β-Xylosides Using a Stable Isotope-Labeled Saccharide Primer

Otsuka Y., Sato T.

Analytical Chemistry (Analytical Chemistry) 90 ( 8 ) 5201 - 5208 2018.04

ISSN 00032700

© 2018 American Chemical Society. The structures and amounts of glycosaminoglycan (GAG) produced by cells have attracted much interest because GAG biosynthesis activity can change in cellular processes such as disease and differentiation. β-Xylosides, also called saccharide primers, have been used as artificial acceptors not only to generate GAG oligosaccharides in cells and tissues but also to investigate their biosynthetic pathways. Various analytical methods have been applied to confirm the structure and amounts of GAG oligosaccharides elongated using saccharide primers, yet sample preparation processes such as solid-phase extraction in analysis can cause experimental error and disrupt accurate comparative quantification of glycosylated products. In this study, we developed a new quantification method using a deuterium-labeled saccharide primer. The "heavy" and "light" primers were chemically synthesized, and priming abilities were confirmed by liquid chromatography-tandem mass spectrometry. Relative peak areas of light/heavy products showed good linearity and were well correlated with the theoretical amounts of glycosylated products. Then, as a validation study, we carried out a biosynthesis inhibition assay using known GAG biosynthesis inhibitors. According to the relative quantification using saccharide primers, differences in the mode-of-action among the four GAG biosynthesis inhibitors were dependent on the GAG biosynthetic pathway. Our results indicate that the method will likely forge a new path for comparative glycosaminoglycomics using cultured cells and tissues.

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Novel hemagglutinin-binding sulfated oligofucosides and their effect on influenza virus infection

Kosono S., Kasai A., Komaba S., Matsubara T., Sato T., Takahashi D., Toshima K.

Chemical Communications (Chemical Communications) 54 ( 54 ) 7467 - 7470 2018

ISSN 13597345

© 2018 The Royal Society of Chemistry. Among a series of chemically synthesized fucoidan derivatives (1-9), 5 was found for the first time to bind to influenza virus hemagglutinins (HAs) and inhibit hemagglutination activity. In addition, a designed C3-symmetric tripodal ligand 10, synthesized with three sulfated oligofucoside moieties of 5, exhibited much greater hemagglutination inhibition activity than 5. A plaque assay using MDCK cells demonstrated that 10 effectively inhibited influenza virus infection.

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Glycoreplica peptides

Matsubara T., Sato T., Taki T.

Methods in Molecular Biology (Methods in Molecular Biology) 1804 437 - 447 2018

ISSN 10643745

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. “Glycoreplica peptides” are prepared using a phage display peptide library and monoclonal antibodies that recognize the carbohydrate epitopes of glycoconjugate antigens. The peptides obtained not only mimic the shapes of original glycoconjugate antigens but also have some of their functions. We herein describe how to identify the amino acid alignments of glycoreplica peptides using phage display selection against carbohydrate-binding proteins. Target-specific peptides and proteins may be selected from the large repertory of a peptide/protein library using phage display technology. Glycoreplica peptides have the potential to become alternatives to carbohydrate ligands such as mimotopes for vaccinations and carbohydrate-derived drugs for carbohydrate-related diseases.

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Amyloid- fibrils assembled on ganglioside-enriched membranes contain both parallel -sheets and turns

Matsubara T., Yasumori H., Ito K., Shimoaka T., Hasegawa T., Sato T.

Journal of Biological Chemistry (Journal of Biological Chemistry) 293 ( 36 ) 14146 - 14154 2018

ISSN 00219258

© 2018 Matsubara et al. Some protein and peptide aggregates, such as those of amyloid- protein (A), are neurotoxic and have been implicated in several neurodegenerative diseases. A accumulates at nanoclusters enriched in neuronal lipids called gangliosides in the presynaptic neuronal membrane, and the resulting oligomeric and/or fibrous forms accelerate the development of Alzheimer’s disease. Although the presence of A deposits at such nanoclusters is known, the mechanism of their assembly and the relationship between A secondary structure and topography are still unclear. Here, we first confirmed by atomic force microscopy that A 40 fibrils can be obtained by incubating seed-free A 40 monomers with a membrane composed of sphingomyelin, cholesterol, and the ganglioside GM1. Using Fourier transform infrared (FTIR) reflection–absorption spectroscopy, we then found that these lipid-associated fibrils contained parallel -sheets, whereas self-assembled A 40 molecules formed antiparallel -sheets. We also found that the fibrils obtained at GM1-rich nanoclusters were generated from turn A 40 . Our findings indicate that A generally self-assembles into antiparallel -structures but can also form protofibrils with parallel -sheets by interacting with ganglioside-bound A. We concluded that by promoting the formation of parallel -sheets, highly ganglioside-enriched nanoclusters help accelerate the elongation of A fibrils. These results advance our understanding of ganglioside-induced A fibril formation in neuronal membranes and may help inform the development of additional therapies for Alzheimer’s disease.

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Size and shape of amyloid fibrils induced by ganglioside nanoclusters: role of sialyl oligosaccharide in fibril formation

Teruhiko Matsubara, Masaya Nishihara, Hanaki Yasumori, Mako Nakai, Katsuhiko Yanagisawa, Toshinori Sato

Langmuir 33 13874 - 13881 2017

Research paper (scientific journal), Joint Work, Accepted

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Selective intracellular delivery of ganglioside GM3-binding peptide through caveolae/raft-mediated endocytosis

SATO TOSHINORI

Biomacromolecules, 18 355 - 362 2017

Research paper (scientific journal), Joint Work, Accepted

Saccharide primers comprising xylosyl-serine primed phosphorylated oligosaccharides act as intermediates in glycosaminoglycan biosynthesis

Yuya Otsuka, Toshinori Sato

ACS Omega 2 3110 - 3122 2017

Research paper (scientific journal), Joint Work, Accepted

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Kohamamides A, B and C, Cyclic Depsipeptides from an Okeania sp. Marine Cyanobacterium

Arihiro Iwasaki, Ikuma Shiota, Shimpei Sumimoto, Teruhiko Matsubara, Toshinori Sato, Kiyotake Suenaga

J. Nat. Prod. 80 1948 - 1952 2017

Research paper (scientific journal), Accepted

Access to Document (DOI)

In vitro and in vivo gene delivery using chitosan/hyaluronic acid nanoparticles: Influences of molecular mass of hyaluronic acid and lyophilization on the transfection efficiency

Toshinori Sato, Mitsuhiro Nakata, Zhihong Yang, Yu Torizuka, Satoko Kishimoto, Masayuki Ishihara

J. Gene Med. 19 e2968 2017

Research paper (scientific journal), Joint Work, Accepted

Design of glycopolymers carrying sialyl oligosaccharides for controlling the interaction with the influenza virus

Masanori Nagao, Yurina Fujiwara, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura

Biomacromolecules 18 4385 - 4392 2017

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Highly sensitive detection of influenza virus by boron-doped diamond electrode terminated with sialic acid-mimic peptide

SATO TOSHINORI

Proc. Natl. Acad. Sci. USA 113 ( 32 ) 8981 - 8984 2016

Research paper (scientific journal), Joint Work, Accepted

Functional Domains of ZFP809 Essential for Nuclear Localization and Gene Silencing,

Yu Ichida, Yuko Utsunomiya Y, Junko Tomikawa, Kazuhiko Nakabayashi, Toshinori Sato, Masafumi Onodera

Biosci. Biotech. Biochem. 80 114 - 120 2016

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

体液類似環境におけるアパタイト結合性ペプチド樹状オリゴマーによるミネラリゼーション

橋詰　峰雄、小野上　綾乃、飯島　一智、松原　輝彦、内田　祐樹、佐藤　智典

高分子論文集 73 55 - 61 2016

Research paper (scientific journal), Accepted

糖鎖模倣ペプチドのインフルエンザヘマグルチニンに対する結合活性の多価効果

松原輝彦、大西愛、齋藤智美、山口大介、佐藤智典

高分子論文集 73 62 - 68 2016

Research paper (scientific journal), Joint Work, Accepted

Heptapeptide Ligands against Receptor-Binding Sites of Influenza Hemagglutinin toward Anti-Influenza Therapy

Teruhiko Matsubara, Ai Onishi, Daisuke Yamaguchi, Toshinori Sato

Bioorg. Med. Chem. 24 1106 - 1114 2016

Research paper (scientific journal), Accepted

Access to Document (DOI)

Binding of Hemagglutinin and Influenza Virus to a Peptide-Conjugated Lipid Membrane

Teruhiko Matsubara, Rabi Shibata, Toshinori Sato

Front. Microbiol. 7 468 2016

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Imbalance in fatty-acid-chain length of gangliosides triggers Alzheimer amyloid deposition in the precuneus, Naoto Oikawa

Teruhiko Matsubara, Ryoto Fukuda, Hanaki Yasumori, Hiroyuki Hatsuta, Shigeo Murayama, Toshinori Sato, Akemi Suzuki, Katsuhiko Yanagisawa

PLOS ONE 10 ( 3 ) e0121356 2015

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Mebamamides A and B, Cyclic Lipopeptides Isolated from the Green Alga Derbesia marina

Arihiro Iwasaki, Osamu Ohno, Shinpei Sumimoto, Teruhiko Matsubara, Satoshi Shimada, Toshinori Sato, and Kiyotake Suenaga

J. Nat. Prod. ７８ 901 - 908 2015

Research paper (scientific journal)

Access to Document (DOI)

Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells

Takuma Ojima, Eri Shibata, Shiho Saito, Masashi Toyoda, Hideki Nakajima, Mayu Yamazaki-Inoue, Yoshitaka Miyagawa, Nobutaka Kiyokawa, Jun-ichiro Fujimoto, Toshinori Sato, and Akihiro Umezawa

Scientific Reports 5 14988 2015

Research paper (scientific journal), Accepted

Access to Document (DOI)

Functional domains of ZFP809 essential for nuclear localization and gene silencing

Yu Ichida, Yuko Utsunomiya, Toru Yasuda, Kazuhiko Nakabayashi, Toshinori Sato, Masafumi Onodera

PLOS ONE 10e0139274 2015

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Synthesis and influenza virus inhibitory activities of carbosilane dendrimers peripherally-functionalized with hemagglutinin-binding peptide

Ken Hatano, Teruhiko Matsubara, Yosuke Muramatsu, Masakazu Ezure, Tetsuo Koyama, Koji Matsuoka, Ryunosuke Kuriyama, Haruka Kori, Toshinori Sato

J. Med. Chem. 57 8332 - 8339 2014

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Antibacterial activity of polyethylenimine/carrageenan multi-layer against pathogenic bacteria

A. V. Briones, T. Sato, Ursela G. Bigol

Advances in Chemical Engineering and Science 4 233 - 241 2014

Research paper (scientific journal), Accepted

Structural Studies on ι-Carrageenan Derived Oligosaccharides and Its Application

A. V. Briones, and T. Sato

Advances in Chemical Engineering and Science 4 17 - 22 2014

Research paper (scientific journal), Accepted

Fragmin/protamine microparticles to adsorb and protect HGF and to functionas local HGF carriers in vivo

Satoko Kishimoto, Masayuki Ishihara, Shingo Nakamura, Masanori Fujita, Megumi Takikawa, Yuki Sumi, Tomoharu Kiyosawa, Toshinori Sato, Yasuhiro Kanatani

Acta Biomaterialia 9 4763–4770 2013

Research paper (scientific journal), Joint Work, Accepted

Involvement of Ext1 and Heparanase in migration of mouse FBJ osteosarcoma cells

Yinan Wang, XiaoYan Yang, Sadako Yamagata, Tatsuya Yamagata, Toshinori Sato

Mol. Cell Biochem. 373 63–72 2013

Research paper (scientific journal), Joint Work, Accepted

In vivo gene transfer using pDNA/chitosan/chondroitin sulfate ternary complexes: Influence of chondroitin sulfate on the stability of freeze-dried complexes and transgene expression in vivo

K. Hagiwara, S. Kishimoto, M. Ishihara, Y. Koyama, O. Mazda, T. Sato

Journal of Gene Medicine 15 83 - 92 2013

Research paper (scientific journal), Accepted

Access to Document (DOI)

The density of GM1 in nanoclusters is a critical factor in the formation of a spherical assembly of amyloid β-protein on synaptic plasma membranes

T. Matsubara, K. Iijima, N. Yamamoto, K. Yanagisawa, T. Sato

Langmuir 29 2258 - 2264 2013

Research paper (scientific journal), Accepted

Access to Document (DOI)

Incorporation of glycosylated amino acid into protein by an in vitro translation system

Teruhiko Matsubara, Kazutoshi Iijima, Takayoshi Watanabe, Takahiro Hohsaka, Toshinori Sato

Bioorganic & Medicinal Chemistry Letters 23 5634 - 5636 2013

Research paper (scientific journal), Accepted

Ability of chitosan/carrageenan complex to encapsulate bovine serum albumin (BSA) for potential use in protein delivery

A. V. Briones, and T. Sato

Asian Journal of Biological and Life Sciences 2 163 - 169 2013

Research paper (scientific journal), Accepted

Involvement of Ext1 and Heparanase in migration of mouse FBJ osteosarcoma cells

Yinan Wang, XiaoYan Yang, Sadako Yamagata, Tatsuya Yamagata, Toshinori Sato

Mol. Cell Biochem. 373 63–72 2012

Research paper (scientific journal), Joint Work, Accepted

Brain insulin resistance accelerates Aß fibrillogenesis by inducing GM1 ganglioside clustering in the presynaptic membranes

Naoki Yamamoto, Teruhiko Matsubara, Kazuya Sobue, Mamoru Tanida,Rika Kasahara, Kaori Naruse, Hideo Taniura, Toshinori Sato, Kenji Suzuki

Journal of Neurochemistry (Journal of Neurochemistry) 121 619-628 2012

Research paper (scientific journal), Joint Work, Accepted

Calcium Phosphate Mineralization Induced by Synthetic Peptides Having Different Distributions in Simulated Body Fluids

Mineo Hashizume, Noriaki Kamaya, Yuki Uchida, Teruhiko Matsubara, and Toshinori Sato

Chem Lett. 41 588-590 2012

Research paper (scientific journal), Joint Work, Accepted

Effective expansion of human adipose-derived stromal cells and bone marrow-derived mesenchymal stem cells cultured on a fragmin/protamine nanoparticles-coated substratum with human platelet-rich plasma

Satoko Kishimoto, Masayuki Ishihara, Yasutaka Mori, Megumi Takikawa, Hidemi Hattori, Shingo Nakamura and Toshinori Sato

J. Tissue Eng. Regen. Med DOI: 10.1002/term.1488 2012

Research paper (scientific journal), Joint Work, Accepted

The effects of coating pDNA/chitosan complexes with chondroitin sulfate on physicochemical characteristics and cell transfection

Kenji Hagiwara, Mitsuhiro Nakata, Yoshiyuki Koyama, Toshinori Sato

Biomaterials 33 7251- 7260 2012

Research paper (scientific journal), Single Work, Accepted

Glycosylation of Nα-lauryl-O-(β-D-xylopyranosyl)-L-serinamide as a Saccharide Primer in Cells

Yinan Wang, Tomoaki Kumazawa, Keisuke Shiba, Kenji Osumi, Mamoru Mizuno, and Toshinori Sato

Carbohydrate Res. 361 33-40 2012

Research paper (scientific journal), Joint Work, Accepted

Carbohydrate recognition by pentadecapeptide ligands for a series of sialylated oligosaccharides

Teruhiko Matsubara, Ai Onishi, Toshinori Sato

Bioorganic & Medicinal Chemistry 20 6452-6458 2012

Research paper (scientific journal), Joint Work

Intricate Recognition of Glycolipid-like Compounds by HIV-1 Envelope Proteins Evaluated with Surface Plasmon Resonance Imaging

Tomoko Okada, Arisa Kimura, Hiroshi Miura, Toshinori Nishiyama, Masako Mori, Jyunya Suzuki, Masayo Ogiso, Koji Matsuoka, Toshinori Sato, Kenichi Hatanaka, and Norihiko Minoura

Journal of Carbohydrate Chemistry 31 584-592 2012

Research paper (scientific journal), Joint Work, Accepted

Calcium regulates caveolin-1 expression at the transcriptional level

Xiao-Yan Yang, Cheng-Cheng Huang, Qi-Ming Kan, YanLi, Dan Liu, Xue-Cheng Zhang, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

Biochem. Biophys. Res. Commun. 426 334-341 2012

Research paper (scientific journal), Joint Work, Accepted

Accelerated biosynthesis of neolactoseries-glycosphingolipids in differentiated mouse embryonal carcinoma F9 cells detected by using dodecyl N-acetylglucosaminide as a saccharide primer

Nao Ogasawara, Yohko U. Katagiri, Nobutaka Kiyokawa, Tomonori Kaneko, Ban Sato, Hideki Nakajima, Yoshitaka Miyagawa, Yasunori Kushi, Hideharu Ishida, Makoto Kiso, Hajime Okita, Toshinori Sato, Junichiro Fujimoto

J Biochem. 149 321-330 2011

Research paper (scientific journal), Joint Work, Accepted

Fragmin/Protamine Microparticles (F/P MPs) as cell carriers enhance the formation and growth of tumors in vivo

Isao Kumano, Satoko Kishimoto, Shingo Nakamura, Hidemi Hattori, Yoshihiro Tanaka, Mitsuhiro Nakata, Toshinori Sato, Masanori Fujita, Tadaaki Maehara, Masayuki Ishihara

Cellular and Molecular Bioengineering 4 476-483 2011

Research paper (scientific journal), Joint Work, Accepted

Ganglioside GD1a negatively regulates hepatocyte growth factor expression through caveolin-1 at the transcriptional level in murine osteosarcoma cells

Lan Zhang, Yinan Wang, Li Wang, Ting Cao, Sumiko Hyuga, Toshinori Sato, Sadako Yamagata,Tatsuya Yamagata

Biochimica et Biophysica Acta 1810 759-768 2011

Research paper (scientific journal), Joint Work, Accepted

GM3 suppresses anchorage-independent growth via Rho GDP dissociation inhibitor beta in melanoma B16 cells

Pu Wang, Su Xu, Yinan Wang, Peixing Wu, Jinghai Zhang, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

Cancer Sci. 102 1476-1485 2011

Research paper (scientific journal), Joint Work, Accepted

GM3 Upregulation of Matrix Metalloproteinase-9 Possibly Through PI3K, AKT, RICTOR, RHOGDI-2, and TNF-A Pathways in Mouse Melanoma B16 Cells

P. Wang P, X. Wang, P. Wu, J. Zhang, T. Sato, S. Yamagata, T. Yamagata

Adv. Exp. Med. Biol. 705 335-348 2011

Research paper (scientific journal), Joint Work, Accepted

Selective Expansion of CD34+ Cells fromMouse Bone Marrow Cultured on LH/PMP-Coated Plates with Adequate Cytokines

S. Kishimoto, M. Ishihara, Y. Kanatani, M. Nambu, M. Takikawa, Y. Sumi, S. Nakamura, Y. Mori, H. Hattori, Y. Tanaka, T. Sato

Journal of Tissue Engineering 1-8 2011

Research paper (scientific journal), Joint Work, Accepted

Physicochemical properties of pDNA/chitosan complexes as gene delivery systems,

Kenji Hagiwara, Riany Anastasia, Mitsuhiro Nakata, Toshinori Sato

Current Drug Discovery Technologies 8 329-339 2011

Research paper (scientific journal), Joint Work, Accepted

糖鎖チップ

佐藤智典

バイオチップ実用化ハンドブック (NTS) 180-186 2010

Research paper (scientific journal), Single Work

Sialic Acid-Mimic Peptides As Hemagglutinin Inhibitors for Anti-Influenza Therapy, T. Matsubara

A. Onishi, T. Saito, A. Shimada, H. Inoue, T. Taki, K. Nagata, Y. Okahata, and T. Sato

J. Med. Chem 53 4441–4449 2010

Research paper (scientific journal), Joint Work, Accepted

Neuroblastoma cells can be classified according to glycosphingolipid expression profiles identified by liquid chromatography-tandem mass spectrometry

T. Kaneko, H. Okita, H. Nakajima, K. Iijima, N. Ogasawara, Y. Miyagawa, Y. U. Katagiri, A. Nakagawa, N. Kiyokawa, T. Sato, J. Fujimoto

Int. J. Oncol. 37 1279-1288 2010

Research paper (scientific journal), Joint Work, Accepted

Acrosome reaction, Steroidal saponin, membrane microdomain, GM1 ganglioside, caveolaebreak assay, sperm capacitation

M. Naruse, H. Suetomo, T. Matsubara, T. Sato, H. Yanagawa, M. Hoshi, M. Matsumoto

Developmental Biology 347 147-153 2010

Research paper (scientific journal), Joint Work, Accepted

Evaluation of pDNA-Polyethylenimine Complex Coated with Iota Carrageenan for In Vitro Gene Delivery

A. V. Briones, T. Sato

ASEAN Journal on Science and Technology for Development 347 147-153 2010

Research paper (scientific journal), Joint Work, Accepted

糖鎖ライブラリーと糖鎖アレイ

佐藤智典

化学フロンティア22「生命現象を理解する分子ツール」 (化学同人) 95-101 2010

Research paper (scientific journal), Single Work, Accepted

Observations of the distribution of GM3 in membrane microdomains by atomic force microscopy

K. Iijima, N. Soga, T. Matsubara and T. Sato

J. Colloid Interface Sci. 337 369-374 2009

Research paper (scientific journal), Joint Work, Accepted

Inhibition of influenza virus infections by sialylgalactose-binding peptides selected from a phage library

T. Matsubara, M, Sumi, H. Kubota, T. Taki, Y. Okahata,T. Sato

Journal of Medicinal Chemistry 52 4247-4256 2009

Research paper (scientific journal), Joint Work, Accepted

Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using capillary electrophoresis-electrospray ionization mass spectrometry

X. Zhu, K. Hatanaka, T. Yamagata, T. Sato

Electrophoresis 30 3519-3526 2009

Research paper (scientific journal), Joint Work, Accepted

5a-Carbaglycopyranoside primers: potential building blocks for biocombinatorial synthesis of glycosphingolipid analogues

H. Aoyama, S. Ogawa, and T. Sato

Carbohydr. Res. 344 2088-2092 2009

Research paper (scientific journal), Joint Work, Accepted

Age-dependent high-density clustering of GM1 ganglioside at presynaptic neuritic terminals promotes amyloid beta-protein fibrillogenesis

N. Yamamoto, T. Matsubara, T. Sato, K. Yanagisawa

Biochim. Biophys. Acta 1778 2717-2726 2008

Research paper (scientific journal), Joint Work, Accepted

In vitro Gene Delivery by pDNA/Chitosan Complexes Coated with Anionic PEG Derivatives That Have a Sugar Side Chain

M. Hashimoto, Y. Koyama, and T. Sato

Chem Lett. 37 266-267 2008

Research paper (scientific journal), Single Work, Accepted

Glycosylation of dodecyl 2-acetamide-2-deoxy-β-D-glucopyranoside and dodecyl β-D-galactopyranosyl-(1-4)-2-acetamide-2-deoxy-β-D-glucopyranoside as saccharide primers in cells

T. Sato, M. Takashiba, R. Hayashi, X. Zhu, T. Yamagata

Carbohydr. Res. 343 831-838 2008

Research paper (scientific journal), Joint Work, Accepted

Selection of carbohydrate-binding domain with a helix-loop-helix structure

T. Matsubara, M. Iida, T. Tsumuraya, I. Fujii, T. Sato

Biochemistry 47 6745-6751 2008

Research paper (scientific journal), Joint Work, Accepted

Ganglioside GD1a suppresses TNFa expression via Pkn1 at the transcriptional level in mouse osteosarcoma-derived FBJ cells

L. Wang, Y. Wang, T. Sato, S. Yamagata, T. Yamagata

Biochem. Biophys. Res. Commun. 371 230-235 2008

Research paper (scientific journal), Joint Work, Accepted

Specific binding of GM1-binding peptides to high-density GM1 in lipid membranes

T. Matsubara, K. Iijima, M. Nakamura, T. Taki, Y. Okahata, T. Sato

Langmuir 23 708-714 2007

Research paper (scientific journal), Joint Work, Accepted

The distinction of underivatized monosaccharides using electrospray ionization ion trap mass spectrometry

Xingyu Zhu and Toshinori Sato

Rapid Communications in Mass Spectrometry 21 191-198 2007

Research paper (scientific journal), Single Work, Accepted

Structural Transition Study of a Fifteen Amino Acid Residue Peptide Induced by GM1

N. Fujitani, H. Shimizu, T. Matsubara, T. Ohta, Y. Komata, N. Muira, T. Sato, and S.-I. Nishimura

Carbohydr. Res. 342 1895-1903 2007

Research paper (scientific journal), Single Work, Accepted

ファージデイスプレイ法

松原輝彦、佐藤　智典

分子間相互作用解析ハンドブック（実験医学別冊） (羊土社) 16-22 2007

Research paper (scientific journal), Joint Work

Identification of Oligosaccharide-Recognition Molecules by Phage-Display Technology

M. Matsubara, T. Sato

Trends In Glycoscience and Glycotechnology 19 133-145 2007

Research paper (scientific journal), Joint Work, Accepted

Syntheses of oligosaccharides using cell function

Toshinori Sato, Kenichi Hatanaka, Hironobu Hashimoto, Tatsuya Yamagata

Trends In Glycoscience and Glycotechnology 19 1-17 2007

Research paper (scientific journal), Joint Work, Accepted

グライコチップ（糖鎖アレイ）

佐藤智典

ナノバイオ計測の実際 (講談社サイエンティフィク) 42-51 2007

Research paper (scientific journal), Single Work

Lactosylated chitosan for DNA delivery into hepatocytes: the effect of lactosylation on the physicochemical properties and intracellular trafficking of pDNA/chitosan complexes

M. Hashimoto, M. Morimoto, H. Saimoto, Y. Shigemasa, T. Sato

Bioconjugate Chemistry 17 309-316 2006

Research paper (scientific journal), Joint Work, Accepted

Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, YoshihiroShigemasa, Hironobu Yanagie, Masazumi Eriguchi and Toshinori Sato

Biotechnology Letters 28 815-821 2006

Research paper (scientific journal), Joint Work, Accepted

Hyaluronic acid and its derivative as a multi-functional gene expression enhancer:Protection from non-specific interactions, adhesion to targeted cells, and transcriptional adhesion

T. Ito, N. Iida-Tanaka, T. Niidome, T. Kawano, K. Kubo, K. Yoshikawa, T. Sato, Z. Yang, Y. Koyama

J. Controlled Rel. 112 382-388 2006

Research paper (scientific journal), Joint Work, Accepted

動物細胞に作らせるオリゴ糖鎖のライブラリー

佐藤智典

日本食品科学工学会誌 53 85-89 2006

Research paper (scientific journal), Single Work, Accepted

A Peptide Motif Recognizing a Polymer Stereoregularity

T. Serizawa, T. Sawada, H. Matsuno, T. Matsubara, T. Sato

J. Am. Chem. Soc. 127 13780-13781 2005

Research paper (scientific journal), Joint Work, Accepted

Display of Azido Glycoside on a Sensor Chip

T. Sato, S. Fujita, M.C. Z. Kasuya, K. Hatanaka, T. Yamagata

Chem Lett 33 580-581 2004

Research paper (scientific journal), Single Work, Accepted

Receptor-independent augmentation of adenovirus-mediated gene transfer with chitosan in vitro

Yosei Kawamata, Yuji Nagayama, Kazuhiko Nakao, Hiroyuki Mizuguchi, Takao Hayakawa, Toshinori Sato, Nobuko Ishii

Biomaterials 23 4573-4579 2002

Research paper (scientific journal), Joint Work, Accepted

Mechanism of Cell Transfection with Plasmid/Chitosan Complexes

T. Ishii, Y. Okahata, T. Sato

Biochim. Biophys. Acta 1514 51-64 2001

Research paper (scientific journal), Joint Work, Accepted

Azido glycoside primer: a versatile building block for the biocombinatorial synthesis of glycosphingolipid analogues

M. C. Z. Kasuya, L. X. Wang, Y. C. Lee, M. Mitsuki, H. Nakajima, Y. Miura, T. Sato, K. Hatanaka, S. Yamagata, T. Yamagata

Carbohydr. Res. 329 755-763 2000

Research paper (scientific journal), Joint Work, Accepted

Selection of Ganglioside GM1-binding Peptides by using Phage Library

T. Matsubara, D. Ishikawa, T. Taki, Y. Okahata, and T. Sato

FEBS Letters ( 456 ) 253-256 1999

Joint Work

Inhibition of 2,7-Dideoxy-7-fluoro-2,3-didehydrosialic Acid for Binding of Influenza Virus H1 Hemagglutinin to GM3

T. Sato, F. Ohtake, Y. Ohira, and Y. Okahata

Chem. Lett. 1999 145-146 1999

Joint Work

In vitro gene delivery mediated by chitosan

T. Sato, T. Ishii, Y. Okahata

Proceed. Int'l Symp. Control. Rel. .Bioact. Mater. ( 26 ) 803-804 1999

Research paper (conference, symposium, etc.), Joint Work

Binding affinity of GM3 lactone for influenza virus, Toshinori Sato

T. Sato, M. Ishii, F. Ohtake, K. Nagata, T. Terabayashi, Y. Kawanishi, and Y. Okahata

Glycoconjugate J. ( 16 ) 223-227 1999

Joint Work

Observation of subcellular distribution of antisense oligonucleotide/lipid complexes by confocal laser scanning microscope

T. Sato, H. Nishi, H. Akino, Y. Okahata, Y. Shoji, J. Shimada

Drug Delivery System ( 13 ) 359-364 1998

Joint Work

Quantitative measurement of the interaction between ganglioside monolayers and wheat germ agglutinin (WGA) by a quartz-crystal microbalance

T. Sato, T. Serizawa, F. Ohtake, M. Nakamura, T. Terabayashi, Y. Kawanishi, and Y. Okahata

Biochim. Biophys. Acta ( 1380 ) 82-92 1998

Joint Work

Morphology and proliferation of B16 melanoma cells in the presence of lanthanoid and Al3+ ions

T. Sato, M. Hashizume, Y. Hotta, and Y. Okahata

BioMetals ( 11 ) 107-112 1998

Joint Work

Cell uptake and transfection efficiency of DNA/glycolipid complexes

T. Sato, T. Ishizuka, and Y. Okahata

Kobunshi Ronbun Shu ( 55 ) 217-224 1998

Joint Work

Selective Bindings of a Lectin for Phase-separated Glycolipid Monolayers

M. Hashizume, T. Sato, and Y. Okahata

Chem. Lett. 399-400 1998

Joint Work

Analysis for the subcellular distribution of oligonucleotide/lipid complexes

T. Sato, H. Nishi, Y. Okahata, Y. Shoji, and J. Shimada

Nucleic Acid Symposim Series ( 37 ) 303-304 1997

Joint Work

Delivery of 10B Atoms for Effective Boron Neutron Capture Therapy using Boronated Polyethylene Glycol-binding BSA

H. Yanagie, T. Sato, H. Nishi, Y. Okahata, Y. Fujii, M. Eriguchi, H. Kobayashi, T. Kobayashi, and K. Ono

SPIE ( 2867 ) 101-104 1997

Joint Work

Characterization, Cell Uptake, and Subcellular Distribution of DNA Complexes with Lipoglutamides Having Tetraethylene Glycol Tails

T. Sato, H. Akino, Y. Okahata, Y. Shoji, and J. Shimada

J. Biomaterial Science, Polym. Ed ( 9 ) 31-42 1997

Joint Work

Binding Affinity of GM3 Lactone to Wheat Germ Agglutinin

T. Sato, M. Ishii, T. Terabayashi, Y. Kawanishi, and Y. Okahata

Chem. Lett. 669-670 1997

Joint Work

Interaction of rare earth ions with saccharides, glycolipid membranes, and animal cells

Toshinori Sato

New Development of Studies on Rare Earth Complexes 615-622 1997

Single Work

A DNA complex with Lipoglutamide and Its Cellular Interaction

T. Sato, H. Akino, Y. Okahata, Y. Shoji, and J. Shimada

Advances in Polymeric Biomaterials Science, CMC Co., Ltd 373-380 1997

Joint Work

Formation of A DNA/polygalactosamine Complex and Its Interaction with Cells

T. Sato, N. Shirakawa, H. Nishi, and Y. Okahata

Chem Lett. 725-726 1996

Joint Work

Binding of Influenza Virus to Monosialoganglioside (GM3) Reconstituted in Glucosylceramide and Sphingomyelin Membranes

T. Sato, T. Serizawa, and Y. Okahata

Biochim. Biophys. Acta ( 1285 ) 14-20 1996

Joint Work

Direct Transfer of Blood Group Antigens from Human Erythrocytes to Liposomes

K. Suzuki, Y. Okumura, T. Sato, T. Yasuda, A. Oki, M. Oki, and J. Sunamoto

Transfusion ( 36 ) 966-968 1996

Joint Work

Organization and Recognition Function of Gangliosides in Lipid Monolayer

T. Sato, T. Serizawa, and Y. Okahata

Biomedical Functions and Biotechnology of natural and Artificial Polymers, ATL Press 145-155 1996

Joint Work

DNA Hybrid Complexes with Glutamic Acid Derivatives and Their Applications to Gene Transfer

T. Sato, T. Kawakami, N. Shirakawa, H. Akino, and Y. Okahata

Biomedical Functions and Biotechnology of Natural and Artificial Polymers, ATL Press 135-144 1996

Joint Work

The Influence of Serum on The Spreading of Tumor Cells on Synthetic Glycolipid Films

T. Sato, M. Endo, and Y. Okahata

Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems, Springer-Verlag Tokyo 273-274 1996

Joint Work

Development of gene delivery system by using DNA complexes and their application to antisense drug

T. Sato and Y. Okahata

Nihon-rinshou ( 54 ) 235-244 1996

Joint Work

Interaction of Rare Earth Ions with Biomembranes

T. Sato

Rare Earth ( 29 ) 49-60 1996

Single Work

Preparation of a DNA Complex with Lipoglutamide having Tetraethylene Glycol Tails and Its Application to DNA Delivery into Tumor Cells

T. Sato, H. Akino, and Y. Okahata

Bull. Chem. Soc. Jpn ( 69 ) 2335-2340 1996

Joint Work

The quartz-crystal microbalance study of protein binding on lipid monolayers at the air-water interface

Y. Okahata, Y. Ebara, and T. Sato

MRS Bulletin ( 20 ) 52-56 1995

Joint Work

Cell Uptake of Albumin with Synthetic Glycolipids

T. Sato, H. Miyaguchi and Y. Okahata

Drug Delivery System ( 10 ) 199-200 1995

Joint Work

Membrane Protein Transfer from Human Erythrocyte Ghosts to Liposomes Containing an Artificial Boundary Lipid

K. Suzuki, Y. Okumura, T. Sato, and J. Sunamoto

Proc. Jpn. Acad ( 71, Ser. B ) 93-97 1995

Research paper (conference, symposium, etc.), Joint Work

Preparation and Characterization of DNA-Lipoglutamate Complexes

T. Sato, T. Kawakami, N. Shirakawa, Y. Okahata

Bull. Chem. Soc. Jpn., ( 68 ) 2709-2715 1995

Joint Work

Formation of A DNA Complex with Lipoglutamide Having Tetraethyleneglycol Tails and Its Interaction with A Tumor Cells

T. Sato, H. Akino, and Y. Okahata

Chem. Lett. 755-756 1995

Joint Work

The Influence of Serum for Spreading of Tumor cells on Synthetic Glycolipid Films

T. Sato, M. Endo, and Y. Okahata

J. Biomaterial Sci., Polym Ed. ( 7 ) 587-599 1995

Joint Work

Physicochemical Perturbation of a-Linolenic Acid Related to Cell Proliferation

H. Fukui, T. Sato, J. Sunamoto

Bull. Chem. Soc. Jpn. ( 67 ) 2213-2218 1994

Joint Work

Recognition of Monosialoganglioside (GM3) Reconstituted in Sphingomyelin and Glucosylceramide Membranes against Wheat Germ Agglutinin. Quantitative Analyses by A Quartz Crystal Microbalance

T. Sato, T. Serizawa and Y. Okahata

Biochem. Biophys. Res. Commun ( 204 ) 551-556 1994

Joint Work

Site-specific Polysaccharide-coated Liposomes and Their Applications in Medicine

T. Sato, N. Moriguchi, K. Akiyoshi, J. Sunamoto, T. Kji, N. Ishii, T. Segawa, and S. Shibata

Proc. First International Conference on Intelligen, Technomic Pub. Co. 419-420 1993

Research paper (conference, symposium, etc.), Joint Work

Anticancer Activity of Polyunsaturated Fatty Acid Emulsion Stabilized by Hydrophobized Plysaccharide

H. Fukui, K. Akiyoshi, T. Sato, J. Sunamoto, S. Yamaguchi, and M. Murata

J. Bioactive and Compatible Polym. ( 8 ) 305-316 1993

Joint Work

Effective Transport of Bioactive Materials to Cell Using Specifically Modified Liposomes

J. Sunamoto, K. Akiyoshi and T. Sato

New Functionality Materials: Design, Preparation and Control, Elsevier Sci. Pub. B. V. 203-210 1993

Joint Work

Site Specific Liposomes as Coated with Polysaccharides

Liposome Technology, CRC Press Vol III, 2nd, CRC Press, 180-198 1993

Joint Work

Effective Reconconstitution of Cell Membrane Proteins into Artificial Cell Liposomes

J. Sunamoto, V. Rosilio, Y. Mori, K. Suzuki, M. Ishitobi, T. Sato and K. Akiyoshi

Progress in pacific Polymer Sciences, Springer 223-233 1992

Joint Work

O/W-Emulsion as Formed by Cholesterol-Bearing Pullulan

S. Yamaguchi, H. Fukui, K. Akiyoshi, T. Sato, and J. Sunamoto

J. Chem. Soc. Jpn., Chem. and Ind. Chem 186-190 1992

Joint Work

Direct Transfer of Tumor Surface Antigic Protein (TSAP) from Tumor Cell to Liposome for Making Liposomal Vcaccine

J. Sunamoto, T. Noguchi, T. Sato, K. Akiyoshi, R. Shibata, E. Nakayama, and H. Shiku

J. Controlled Release ( 20 ) 143-154 1992

Joint Work

Direct Transfer of Membrane Proteins from B16 melanoma Cell to Artificial Cell Liposome

J. Sunamoto, Y. Mori, T. Sato

Proc. Japan Acad. ( 68, Ser. B ) 69-74 1992

Research paper (conference, symposium, etc.), Joint Work

Development of Liposome for targeting Therapy against Brain Tumor

S. Shibata, N. Ryu, A. Ochi, T. Jinnouchi, T. Sato, J. Sunamoto, M. Hirai, K. Matsuyama

Drug Delivery System ( 7 ) 109-114 1992

Joint Work

Naturally Occurring Polysaccharide Derivatives Which Behave as an Artificial Cell Wall on an Artificial Cell Liposome

J. Sunamoto, T. Sato, T. Taguchi, H. Hamazaki

Macromolecules ( 25 ) 5665-5670 1992

Joint Work

Effect of1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its toxic metabolites on the physicochemical property of the lipisomal membrane in relation to their cytochrome P-450 inhibition

N. P. Das, G. S. Shahi,S. M. Moochhala, T. Sato and J. Sunamoto

Chemistry and Physics of Lipids ( 62 ) 303-310 1992

Joint Work

Polysaccharide-Coated Liposomal Amphotericin B for the Treatment of Murine Pulmonary Candidiasis

T. Miyazaki, S. Kohno, K. Sasaki, Y. Inoue, K. Hara, M. Ogasawara, T. Sato and J. Sunamoto

Tohoku J. Exp. Med ( 168 ) 483-490 1992

Joint Work

Development of Functionalized liposome

T. Sato and J. Sunamoto

Shikoku J. Clin. Chem ( 9 ) 31-36 1992

Joint Work

Recent Aspects in the Use of Liposomes in Biotechnology and Medicine

T. Sato, J. Sunamoto

Prog. Lipid Res. ( 31 ) 345-372 1991

Joint Work

Damage of Egg Phosphatidyicholine Liposomes by DNA-Binding Cytotoxic Agents

C. J. O'Connor, W. A. Denny, D. P. Emery, H. Tank, T. Sato, J. Sunamoto

Bull. Chem. Soc. Japan ( 64 ) 1364-1369 1991

Joint Work

Priming for In Vitro and In Vivo Anti-Human T Lymphotropic Virus Type I Cellular Immunity by Virus Related Protein Reconstituted into Liposome

Y. Noguchi, T. Noguchi, T. Sato, Y. Yokoo, S. Itoh, M. Yoshida, T. Yoshiki, K. Akiyoshi, J. Sunamoto, E. Nakayama, and H. Shiku

J. Immunol ( 148 ) 3599-3603 1991

Joint Work

Induction of In Vitro and In Vivo Anti-tumor Responses by Sensitization of Mice With Liposomes Containing a Crude Butanol Extract of Leukemia Cells and Transferred Intermembraneously with Cell Surface Proteins

R. Shibata, T. Noguchi, T. Sato, K. Akiyoshi, J. Sunamoto, H. Shiku, and E. Nakayama

Int. J. Cancer ( 48 ) 434-442 1991

Joint Work

Hepatoma Cell Targetability of Liposomes Coated with Polysaccharides bearing galactose groups

M. Yamamoto, T. Segawa, T. Iwata, T. Kadohara, K. Izawa, T. Tsunoda, R. Tsutiya, S. Gondo, Y. Oohata, N. Ishii, T. KOuji, N. Moriguti, T. Sato, K. Akiyoshi, J. Sunamoto

Drug Delivery System ( 6 ) 355-359 1991

Joint Work

Effective Transfer of Membrane Proteins from Intact Cells to Liposomes and Preparation of Liposomal Vaccines

J. Sunamoto, K. Akiyosi, M. Goto, T. Noguchi, T. Sato, E. Nakayama, R. Shibata, H. Shiku

Ann. New York Acad. Sci. (Enzyme Engineering X) ( 613 ) 116-127 1990

Joint Work

Targeting Chemotherapy of Brain Tumor Using Liposome-Encapsulated Cisplatin - Part 1. Substances Associated With Liposomes to Target Brain Tumor

S. Shibata, T. Jinnouchi, A. Ochi, K. Mori, T. Sato, J. Sunamoto

Drug Delivery System ( 5 ) 83-87 1990

Joint Work

Cell Specificity of Polysaccharide Derivatives on Liposomal Surface

K. Akiyoshi, H. Takanabe, T. Sato, T. Sato, H. Kondo, and J. Sunamoto

Chem. Lett 473-476 1990

Joint Work

Synthesis and Characterization of 1,2-Dimyristoylamido-1,2-deoxyphosphatidylcholine as a Boundary Lipid Model.

J. Sunamoto, M. Goto, K. Iwamoto, H. Kondo, and T. Sato

Biochim. Biophys. Acta ( 1024 ) 209-219 1990

Joint Work

Targeting Chemotherapy of Brain Tumor Using Liposome-Encapsulated Cisplatin Part 2. Pullulan-Coated Liposomes to Target Brain Tumor

A. Ochi, S. Shibata, K. Mori, T. Sato, J. Sunamoto

Drug Delivery System ( 5 ) 261-265 1990

Joint Work

Targeting

J. Sunamoto and T. Sato

Koubunshi-Shinsozai-Binran, Ed by The Society of Polymer Science 340-343 1989

Research paper (scientific journal), Joint Work

Improved Drug Delivery Directed to Specific Tissue Using Polysaccharide-Coated Liposomes

J. Sunamoto and T. Sato,

Multiphase Bio-medical Materials, VNUScience Press, The Netherland 167-190 1989

Joint Work

Development of Cell-Specific Liposome and Its Application in Biotechnology

J. Sunamoto and T. Sato

J. Chem. Soc. Japan, Chem. And Ind. Chem. 161-173 1989

Joint Work

Physicochemical Stabilization of Lipid Microspheres by Coating with Polysaccharide Derivatives

A. Carlsson, T. Sato, and J. Sunamoto

Bull. Chem. Soc. Japan ( 62 ) 791-796 1989

Joint Work

Potent Immunomodulating Activities of Polyvinyladenine and (Vinyladenine-Alt-Maleic Acid) Copolymer

M. Akashi, H. Iwasaki, N. Miyauchi, T. Sato, J. Sunamoto, and K. Takemoto

J. Bioactive and Compatible Polym ( 4 ) 124-136 1989

Joint Work

Cell Targetability of Liposomes Bearing Molecular Recognition Site

T. Sato, K. Akiyoshi, and J. Sunamoto

Drug Delivery System ( 4 ) 7-11 1989

Joint Work

Cell Specific Drug Release from Liposomes

J. Sunamoto, K. Akiyoshi, and T. Sato

Topics in Pharmaceutical Sciences, Amsterdam Medical Press, Noordwijk, The Netherlands 25-38 1989

Joint Work

Targetability of Cell-Specific Liposomes Coated with Polysaccharide-Cholesterol Derivatives

T. Sato

Japanese J. Clin. Med ( 47 ) 1402-1407 1989

Single Work

Serum Free Culture of Mammalian Cells By both Biocompatible Polymers and Artificial Cell Liposomes

T. Sato

Nagasaki Sentan Gijyutu Seika Houkoku Sho ( 6 ) 1-7 1988

Research paper (other academic), Single Work

Molecular Recognition of Polysaccharide-Coated Liposomes, Importance of Sialic Acid Moiety on Liposomal Surface

J. Sunamoto, K. Sakai, T. Sato, and H. Kondo

Chem. Lett 1781-1784 1988

Joint Work

Polysaccharide-Coated Immunoliposomes Bearing Anti-CEA Fab' Fragment and Their Internalization by CEA-Producing Tumor Cells

T. Sato, J. Sunamoto, N. Ishii, and T. Koji

J. Bioactive and Compatible Polym. ( 3 ) 195-204 1988

Joint Work

Polysaccharide-Coated Liposome with Antimicrobial Agents Against Intracytoplasmic Pathogen and Fungus

S. Kohno, T. Miyazaki, K. Yamaguchi, H. Tanaka, T. Hayashi, M. Hirota, A. Saito, K. Hara, T. Sato, and J. Sunamoto

J. Bioactive and Compatible Polym ( 3 ) 137-147 1988

Joint Work

Targeting Cancer Therapy in Mice by Use of Newly Developed Immunoliposomes Bearing Adriamycin

M. Hirota, K. Fukushima, K. Hiratani, J. Kadota, K. Kawano, M. Oka, A. Tomonaga, K. Hara. T. Sato, and J. Sunamoto

J. Liposome Research ( 1 ) 15-33 1988

Joint Work

Improvement of Immunopotentiator Activity of Polyanionic Polymers by Encapsulation into Polysaccharide-Coated Liposome

R. M. Ottenbrite, J. Sunamoto, T. Sato, K. Kojima, K. Sahara, K. Hara, and M. Oka

J. Bioactive and Compatible Polym ( 3 ) 184-190 1988

Joint Work

Development of Novel Immunomodulator and Macrophage Activation Using Phagocyte-directing Liposomes Bearing Poly-anionic Polymer Immunomodulators

J. Sunamoto, T. Sato, K. Hara, A. Tomonaga, and M. Oka

Mochida Memorial Foundation ( 2 ) 163-170 1987

Research paper (bulletin of university, research institution), Joint Work

Lectin-Induced Aggregation of Glycophorin-Reconstituted Liposomes

J. Sunamoto, M. Goto, M. Arakawa, T. Sato, and H.Kondo

J. Chem. Soc. Japan 569-574 1987

Joint Work

A Newly Developed Immunoliposome - An Egg Phosphatidyl-choline Liposome Coated with Pullulan Bearing Both a Cholesterol Moiety and an IgMs Fragment

J. Sunamoto, T. Sato, M. Hirota, K. Fukushima, K. Hiratani, and K. Hara

Biochim. Biophys. Acta ( 898 ) 323-330 1987

Joint Work

Specific Rejection of Glycophorin-Reconstituted Liposomes by Human Phagocytes

M. Goto, M. Arakawa, T. Sato, H. Kondo, and J. Sunamoto

Chem. Lett 1935-1938 1987

Joint Work

Enhanced Biological Activity of Polymeric Drugs by Encap-sulating in Liposomes

J. Sunamoto, T. Sato, and R. M. Ottenbrite

Current Topics in Polymer Science, Hanser Publishers Vol.1 101-115 1987

Joint Work

Studies on The Development of Biocompatible Artificial Cells

J. Sunamoto and T. Sato

Res. Report of Suzuken Memorial Foundation ( 4 ) 77-82 1986

Research paper (scientific journal), Joint Work

Effective Encapsulation of Liposomes by Biocompatible Hydrogels

Sato, S. Takei, and J. Sunamoto

Reports of the Faculty of Engineering, Nagasaki University ( 16 ) 183-192 1986

Joint Work

Macrophage Activation by Poly(maleic acid-alt-2-cyclohexyl-1,3-dioxap-5-ene) as Encapsulated in Polysaccharide-coated Liposome

T. Sato, K. Kojima, T. Ihda, J. Sunamoto, and R. M. Ottenbrite

J. Bioactive and Compatible Polymer ( 1 ) 448-460 1986

Joint Work

Targeting Therapy of Human Tumor Cell Line Xenograpft in Nude Mice Using Pullulan-Coated Liposome Encasulated with Adriamycin

M. Hirota, K. Fukushima, K. Hiratani, K. Kawano, M. Oka, A, Tomonaga, A. Saito, K. Hara, T. Sato, and J. Sunamoto

Jpn. J. Cancer and Chemotherapy ( 13 ) 2875-2878 1986

Joint Work

Utilization of Functionalized Liposomes as an Improved Artificial Cell

J. Sunamoto, H. Kondo and T. Sato

Rep. Asahi Glass Found. Ind. Technol ( 48 ) 231-239 1986

Research paper (bulletin of university, research institution), Joint Work

Development of Monoclonal Antibody-Coupled Liposome Encapsulating Antitumor Drug and Its Medical Application

K. Hara, M. Hirota, K. Fukushima, J. Kadota, K. Hiratani, K. Kohno, M. Oka, A. Tomonaga, T. Sato, and J. Sunamoto

Advances in Cancer Treatment ( 5 ) 89-99 1985

Joint Work

Molecular Complexes of Anionic Porphyrin and Anionic Aromatics

T. Sato, T. Ogawa, and K. Kano

Journal of Physical Chemistry ( 88 (16) ) 3678-3682 1984

Joint Work

Evidence of Stacking of Cationic Porphyrin in Aqueous Solution

K. Kano, T. Miyake, K. Uomoto, T. Sato, T. Ogawa, and S. Hashimoto

Chemistry Letters 1867-1870 1983

Joint Work

Fluorescence Quenching of 5, 10, 15, 20-Tetra(p-tolyl)porphyrin and Zinc Complex by Quinones. Charge-Transfer Interaction and Transient Effect

S. Yamada, T. Sato, K. Kano, and T. Ogawa

Photochemistry and Photobiology ( 37 ) 257-262 1983

Joint Work

Fluorescence Quenching of Water-Soluble Porphyrins. A Noble Fluorescence Quenching of Anionic Porphyrin by Anionic Anthraquinone4

K. Kano, T. Sato, S. Yamada, and T. Ogawa

Journal of Physical Chemistry ( 87 ) 566-569 1983

Joint Work

Spectroscopic Study of Covalently Linked Bisporphyrin

S. Yamada, T. Sato, K. Kano, and T. Ogawa,

Engineering Science Reports Kyushu University ( 4 ) 49-52 1982

Joint Work

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