川上 了史 (カワカミ ノリフミ)

Kawakami, Norifumi

写真a

所属(所属キャンパス)

理工学部 生命情報学科 (矢上)

職名

専任講師

外部リンク

経歴 【 表示 / 非表示

  • 2009年04月
    -
    2013年03月

    名古屋大学, 物質科学国際研究センター

  • 2013年04月
    -
    2014年03月

    名古屋大学, 大学院理学研究科生命理学専攻

  • 2014年04月
    -
    2017年03月

    慶應義塾大学, 理工学部

  • 2017年04月
    -
    2020年03月

    慶應義塾大学, 理工学部, 専任講師(有期)

  • 2020年04月
    -
    継続中

    慶應義塾大学, 理工学部

学歴 【 表示 / 非表示

  • 2002年04月
    -
    2004年03月

    宇部工業高等専門学校, 専攻科物質科学専攻

    高等専門学校, 修了

  • 2004年04月
    -
    2006年03月

    広島大学, 大学院理学研究科博士課程前期

    博士前期

  • 2006年04月
    -
    2009年03月

    広島大学, 大学院理学研究科博士課程後期

    博士後期

学位 【 表示 / 非表示

  • 博士(理学), 広島大学, 課程, 2009年03月

免許・資格 【 表示 / 非表示

  • 放射線取扱主任者第一種, 2015年03月

 

研究分野 【 表示 / 非表示

  • 自然科学一般 / 地球生命科学 (金属イオン、金属タンパク質、実験室進化)

  • ナノテク・材料 / 生物分子化学 (タンパク質、ナノ粒子、ハイドロゲル)

研究テーマ 【 表示 / 非表示

  • 生命の元素に対する進化的可塑性の解明, 

    2015年04月
    -
    継続中

  • タンパク質を鋳型とした分子構造の設計と材料への応用, 

    2014年03月
    -
    継続中

 

論文 【 表示 / 非表示

  • Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60

    Obata J., Kawakami N., Tsutsumi A., Nasu E., Miyamoto K., Kikkawa M., Arai R.

    Chemical Communications (Chemical Communications)  57 ( 79 ) 10226 - 10229 2021年10月

    ISSN  13597345

     概要を見る

    Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self-assembled from fusion proteins of a pentameric Sm-like protein and a dimeric MyoX-coil domain. Herein, we report the icosahedral 60-meric structure of TIP60 solved using single-particle cryo-electron microscopy. Interestingly, the structure revealed 20 regular-triangle-like pores on the surface. TIP60 and its mutants have many modifiable sites on their exterior and interior surfaces. The TIP60 architecture will be useful in the development of biomedical and biochemical nanoparticles/nanocages for future applications.

  • Nanopore-Controlled Dual-Surface Modifications on Artificial Protein Nanocages as Nanocarriers

    Nasu E., Kawakami N., Miyamoto K.

    ACS Applied Nano Materials (ACS Applied Nano Materials)  4 ( 3 ) 2434 - 2439 2021年03月

     概要を見る

    Chemical modification of the interior and exterior surfaces of protein nanocages holds promise for various applications such as cosmetics, pharmaceuticals, and catalysts. However, dual-surface modification of these surfaces using different chemicals remains challenging, particularly when the same substituents, such as cysteine thiols, are modified. We recently produced an artificial protein nanocage called TIP60 that has 20 large surface pores. Chemical modification of cysteine residues introduced in the interior surface of TIP60 showed that these pores allow the passage of small molecules from the outside environment to the inside of the nanocage. In this study, we found that the surface pores on TIP60 function as size-dependent molecular filters. Modification experiments using different-sized polymers containing maleimide groups, which specifically react with thiols, showed that macromolecules with diameters larger than that of the pores could not penetrate into the inner cavity. This molecular size discrimination by the pores prompted us to perform stepwise dual-surface functionalization of a double mutant of TIP60 presenting cysteine residues on the interior and exterior surfaces. This was achieved by modifying the exterior cysteine residues with a polymer containing a maleimide group that cannot penetrate to the inside of the nanocage, followed by modification of the interior cysteine residues using thiol-containing small molecules. Dual-functionalized TIP60 released internal small molecules in a redox-responsive manner. This simple approach for dual-surface modification would make TIP60 a useful nanocarrier for a broad range of applications including drug-delivery and molecular filtration systems.

  • Efficient Degradation of Poly(ethylene terephthalate) with Thermobifida fusca Cutinase Exhibiting Improved Catalytic Activity Generated using Mutagenesis and Additive-based Approaches

    Furukawa M., Kawakami N., Tomizawa A., Miyamoto K.

    Scientific Reports (Scientific Reports)  9 ( 1 )  2019年12月

     概要を見る

    Cutinases are promising agents for poly(ethylene terephthalate) (PET) bio-recycling because of their ability to produce the PET monomer terephthalic acid with high efficiency under mild reaction conditions. In this study, we found that the low-crystallinity PET (lcPET) hydrolysis activity of thermostable cutinase from Thermobifida fusca (TfCut2), was increased by the addition of cationic surfactant that attracts enzymes near the lcPET film surface via electrostatic interactions. This approach was applicable to the mutant TfCut2 G62A/F209A, which was designed based on a sequence comparison with PETase from Ideonella sakaiensis. As a result, the degradation rate of the mutant in the presence of cationic surfactant increased to 31 ± 0.1 nmol min−1 cm−2, 12.7 times higher than that of wild-type TfCut2 in the absence of surfactant. The long-duration reaction showed that lcPET film (200 μm) was 97 ± 1.8% within 30 h, the fastest biodegradation rate of lcPET film thus far. We therefore believe that our approach would expand the possibility of enzyme utilization in industrial PET biodegradation.

  • Acceleration of Enzymatic Degradation of Poly(ethylene terephthalate) by Surface Coating with Anionic Surfactants

    Furukawa M., Kawakami N., Oda K., Miyamoto K.

    ChemSusChem (ChemSusChem)  11 ( 23 ) 4018 - 4025 2018年12月

    ISSN  18645631

     概要を見る

    Enzymatic degradation of poly(ethylene terephthalate) (PET) is promising because this process is safer than conventional industrial approaches. Recently, a cationic PET hydrolase (PETase) was identified from Ideonella sakaiensis. Pre-incubation of a low-crystallinity PET film with anionic surfactants prior to initiating the reaction was found to improve PETase activity 120-fold. After 36 h at 30 °C, the film thickness decreased by 22 %. The binding of surfactants to the film makes the surface anionic, thereby attracting the cationic PETase. Mutagenesis of PETase showed that the surface cationic region formed by R53, R90, and K95, which are located on the same side as the substrate binding pocket, was crucial for efficient acceleration of activity by the anionic surfactant. Thus, surfactant bound on PET aligns the orientation of the active site to the surface, resulting in efficient hydrolysis. We believe that this approach using PETase could be further improved by designing surfactant molecules for the more efficient enzymatic PET degradation.

  • Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces

    川上 了史

    Angewandte Chemie - International Edition (Angewandte Chemie - International Edition)  57 ( 38 ) 12400 - 12404 2018年09月

    研究論文(学術雑誌),  ISSN  1433-7851

     概要を見る

    Protein-based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano-building block for further molecular assembly.

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競争的研究費の研究課題 【 表示 / 非表示

  • 実験室進化で生じた大腸菌変異株の鉄輸送体に着目した進化プロセスの検証

    2022年04月
    -
    2024年03月

    補助金,  研究代表者

  • 大腸菌実験室進化を通じた生物の元素利用に対する進化的可塑性の検討

    2021年07月
    -
    2024年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 川上 了史, 挑戦的研究(萌芽), 補助金,  研究代表者

  • 人工球状タンパク質超分子を用いたナノスケール相分離の実現とその応用

    2018年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 川上 了史, 基盤研究(C), 補助金,  研究代表者

受賞 【 表示 / 非表示

  • 第8回新化学技術研究奨励賞 新化学技術推進協会

    2019年06月

    受賞区分: 出版社・新聞社・財団等の賞

  • 日本蛋白質科学会若手奨励賞優秀賞

    2017年06月

 

担当授業科目 【 表示 / 非表示

  • 生命情報特別講義第2

    2022年度

  • 生命情報輪講

    2022年度

  • 自然科学実験

    2022年度

  • 現代生物学概論

    2022年度

  • 生体反応化学(生命化学第4)

    2022年度

全件表示 >>

担当経験のある授業科目 【 表示 / 非表示

  • 生体反応論第2

    慶應義塾

    2017年04月
    -
    継続中

    春学期