Papers - Tsukada, Kosuke

Simultaneous mapping of unevenly distributed tissue hypoxia and vessel permeability in tumor microenvironment

Keitaro Oda, Yuichiro Iwamoto, Kosuke Tsukada

Biomedical Physics & Engineering Express (IOP Science)  2 ( 6 ) 065017 2016.12

Research paper (scientific journal), Joint Work, Accepted

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Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage

Mami Ishikawa, Mayumi Kajimura, Takayuki Morikawa, Kosuke Tsukada, Toshiyuki Tsuji, Gen Kusaka, Yuichi Tanaka, Makoto Suematsu

Journal of the Neurological Sciences (Elsevier)  368   326 - 333 2016.09

Research paper (scientific journal), Joint Work, Accepted

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Facile design of plant-oil-infused fine surface asperity for transparent blood-repelling endoscope lens

Sachiko Nishioka, Mizuki Tenjimbayashi, Kengo Manabe, Takeshi Matsubayashi, Ken Suwabe, Kosuke Tsukada, Seimei Shiratori

RSC Advances (Royal Society of Chemistry)  6 ( 53 ) 47579 - 47587 2016.09

Research paper (scientific journal), Joint Work, Accepted,  ISSN  2046-2069

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Minimally invasive medical operations, especially endoscope operations, have attracted much attention and play a major role in modern medicine. Endoscope operations are superior to decrease incisions, enabling good post-operation progress. However, during its implementation, blood adheres to the lens of the endoscope, resulting in obstructed vision. This prolongs the operation time and causes the patient to gain weight. Hence, we developed a blood-repelling and transparent material for coating the surface of an endoscope lens. The coating material was produced from plant oil and a rough material for trapping the oil. Edible plant oil was particularly used to enable application to medical devices. A fine surface asperity was achieved by a one-dip treatment, which also enhanced the capillary force and durability of the oil under a water shower. The application of the developed coating material to an endoscope lens in an animal experiment enabled the effective repulsion of blood and other body fluids, the maintenance of a clear vision, and high transmittance. The developed coating material promises to contribute to the achievement of antifouling surfaces in medical devices.

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Cortical microcirculatory disturbance in the super acute phase of subarachnoid hemorrhage - In vivo analysis using two-photon laser scanning microscopy

Mami Ishikawa, Mayumi Kajimura, Takayuki Morikawa, Kosuke Tsukada, Toshiyuki Tsuji, Gen Kusaka, Yuichi Tanaka, Makoto Suematsu

JOURNAL OF THE NEUROLOGICAL SCIENCES (ELSEVIER SCIENCE BV)  368   326 - 33 2016.09

Accepted,  ISSN  0022-510X

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Objective: Subarachnoid hemorrhage (SAH) causes cerebral ischemia and drastically worsens the clinical status at onset. However, the arterial flow is surprisingly well maintained on the cerebral surface. We investigated cortical microcirculatory changes in the super acute phase of SAH using two-photon laser scanning microscopy (TPLSM).
Methods: SAH was induced at the skull base in 10 mice using a prone endovascular perforation model. Before SAH, and I, 2, 5,10, 20,30 and 60 min after SAH, the cortical microcirculation was observed with TPLSM through a cranial window. Diameters of penetrating and precapillary arterioles were measured and red blood cell (RBC) velocities in precapillary arterioles were analyzed using a line-scan method after administration of Q-dot 655 nanocrystals.
Results: One minute after SAH, RBC velocity and flow in precapillary arterioles drastically decreased to <20% of the pre-SAH values, while penetrating and precapillary arterioles dilated significantly. Subsequently, the arterioles either dilated or constricted inconsistently for 60 min with continual decreases in RBC velocity and flow in the arterioles, suggesting neurovascular dysfunction.
Conclusion: SAH caused sudden worsening of the cortical arteriolar velocity and flow at onset. The neurovascular unit cannot function sufficiently to maintain cortical microcirculatory flow in the super acute phase of SAH. (C) 2016 Elsevier B.V. All rights reserved.

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Leukocyte plugging and cortical capillary flow after subarachnoid hemorrhage

Mami Ishikawa, Mayumi Kajimura, Takayuki Morikawa, Kosuke Tsukada, Toshiyuki Tsuji, Gen Kusaka, Yuichi Tanaka, Makoto Suematsu

Acta Neurochir 158 ( 6 ) 1057 - 1067 2016.04

Research paper (scientific journal), Joint Work, Accepted

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An in vitro hepatic zonation model with a continuous oxygen gradient in a microdevice

Sato A., Kadokura K., Uchida H.,Tsukada K.

Biochemical and Biophysical Research Communications (ACADEMIC PRESS INC ELSEVIER SCIENCE)  453 ( 4 ) 767 - 771 2014.10

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0006-291X

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In a hepatic lobule, different sets of metabolic enzymes are expressed in the periportal (PP) and pericentral (PC) regions, forming a functional zonation, and the oxygen gradient is considered a determinant of zone formation. It is desirable to reproduce lobular microenvironment in vitro, but incubation of primary hepatocytes in conventional culture dishes has been limited at fixed oxygen concentrations due to technical difficulties.
We designed a cell culture microdevice with an oxygen gradient to reproduce the hepatic microenvironment in vitro. The oxygen gradient during cell culture was monitored using a laser-assisted phosphorescence quenching method, and the cellular oxygen consumption rate could be estimated from changes in the gradient. Culture medium was continuously exchanged through microchannels installed in the device to maintain the oxygen gradient for a long term without transient hyper-oxygenation.
The oxygen consumption rates of hepatocytes at 70.0 mmHg and 31.4 mmHg of partial oxygen pressure, which correspond to PP and PC regions in the microdevice, were 3.67 x 10(-10) and 3.15 x 10(-10) - mol/s/10(6) cells, respectively. Antimycin A changed the oxygen gradient profile, indicating that cellular respiration can be estimated during cell culture. RT-PCR analysis of hepatocytes cultured under the oxygen gradient showed that mRNA expression of PEPCK and GK significantly increased in culture areas corresponding to PP and PC regions, respectively.
These results indicate that the developed microdevice can reproduce the hepatic lobular microenvironment. The oxygen gradient in the microdevice can be closely controlled by changing the sizes of gas channels and the ambient oxygen concentration around the device; therefore, it could be expected to mimic the oxygen gradient of various organs, and it may be applicable to other pathological models. (C) 2014 Elsevier Inc. All rights reserved.

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Optical scanning of tissue oxygen tension and hypoxia imaging in solid tumors

Yamada R., Tabuchi S., Tsukada K.

Advanced Biomedical Engineering 3   65 - 71 2014.06

Research paper (scientific journal), Joint Work, Accepted

A carbon monoxide sensing film based on hemoglobin allostery

Takada Y, Otsuka R, Tsukada K

Journal of Biomedical Science and Engineering 7 ( 4 ) 173-180 2014.03

Research paper (scientific journal), Joint Work, Accepted

Generation of an oxygen gradient in a microfluidic device and cellular analysis in hypoxia

Uchida H, Sato A, Miyayama A, Tsukada K

Advanced Biomedical Engineering 2   143-149 2014.02

Research paper (scientific journal), Joint Work, Accepted,  ISSN  2187-5219

Near-infrared laser adjuvant for influenza vaccine

Kashiwagi S, Yuan J, Forbes B, Hibert ML, Lee EL, Whicher L, Goudie C, Yang Y, Chen T, Edelblute B, Collette B, Edington L, Trussler J, Nezivar J, Leblanc P, Bronson R, Tsukada K, Suematsu M, Dover J, Brauns T, Gelfand J, Poznansky MC.

PLoS One 8 ( 12 ) e82899 2013.12

Research paper (scientific journal), Joint Work, Accepted

Hypoxia-inducible factor-1 is a determinant of lobular structure and oxygen consumption in the liver

Tsukada K., Tajima T., Hori S., Matsuura T., Johnson RS, Goda N. and Suematsu M.

Microcirculation 20 ( 5 ) 385-393 2013.07

Research paper (scientific journal), Joint Work, Accepted

腫瘍の低酸素イメージングと酸素化過程における時空間解析

山田 遼, 堀之内宏久, 塚田孝祐

電気学会論文誌C（電子・情報・システム部門誌） (電気学会)  132 ( 10 ) 1602-1607 2012

Research paper (scientific journal), Joint Work, Accepted

Co-culture microdevice with oxygen gradient for tumor microenvironment model and metastasis imaging

Shiwa T., Uchida H., and Tsukada K.

American Journal of Biomedical Engineering 2 ( 4 ) 175-180 2012

Research paper (scientific journal), Joint Work, Accepted

Visualization and analysis of blood flow and oxygen consumption in hepatic microcirculation: application to an acute hepatitis model

Tsukada K. and Suematsu M.

Journal of Visualized Experiments  2012

Research paper (scientific journal), Joint Work, Accepted

Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice.

Ryo Yamada, Hirohisa Horinouchi, and Tsukada Kosuke

Conf Proc IEEE Eng Med Biol Soc. 2011   5965-5968 2011

Research paper (conference, symposium, etc.), Joint Work, Accepted

Fenofibrate, a peroxisome proliferator-activated receptor alpha agonist, improves hepatic microcirculatory patency and oxygen availability in a high-fat-diet-induced fatty liver in mice.

Kondo K., Sugioka T., Tsukada K., Aizawa M., Takizawa M., Shimizu K., Morimoto M., Suematsu M. and Goda N.

Advances in Experimental Medicine and Biology (SPRINGER-VERLAG BERLIN)  662   77-82 - 82 2010

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0065-2598

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Nonalcoholic fatty liver disease (NAFLD) is a common disease of chronic liver diseases. Peroxisome proliferator-activated receptor alpha (PPAR alpha) has been implicated to play important roles in the development of the disease. Beyond its effects on lipid metabolisms, PPAR alpha activation in the vascular system has emerged as an attractive therapeutic potential for NAFLD, although its actions in the microcirculatory system are not fully understood. In this study, we investigated the effects of fenofibrate, a PPAR alpha synthetic agonist, on hepatic microcirculation in a high-fat diet (HFD)-induced fatty liver in mice. In vivo imaging analysis revealed the adverse effects of HFD on hepatic vasculature with narrowing of hepatic sinusoids and hepatic microcirculatory perfusion. Oxygen tension was significantly decreased in portal venules, while NADH autofluorescence in hepatocytes was greatly elevated. Fenofibrate treatment remarkably improved microvascular patency, tissue oxygenation and redox states in the affected liver. These results suggest beneficial roles of PPAR alpha activated by fenofibrate on the regulation of both lipid metabolisms and microvascular environments of oxygen metabolism in HFD-induced fatty liver.

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Phosphorescence-assisted microvascular O(2) measurements reveal alterations of oxygen demand in human metastatic colon cancer in the liver of superimmunodeficient NOG mice.

Handa K., Ohmura M., Nishime C., Hishiki T., Nagahata Y., Kawai K., Suemizu H., Nakamura M., Wakui M., Kitagawa Y., Suematsu M., Tsukada K.

Advances in Experimental Medicine and Biology 662   423-9 2010

Research paper (scientific journal), Joint Work, Accepted

T-state stabilization of hemoglobin by nitric oxide to form alpha-nitrosyl heme causes constitutive release of ATP from human erythrocytes.

Akatsu T., Tsukada K., Hishiki T., Suganuma K., Tanabe M., Shimazu M., Kitagawa Y., Yachie-Kinoshita A. and Suematsu M.

Advances in Experimental Medicine and Biology 662   109-14 2010

Research paper (scientific journal), Single Work, Accepted

NOによる腫瘍血管構造の正常化と放射線治療

塚田 孝祐，福村 大

血液・腫瘍科 57 ( 6 ) 652-657 2008

Research paper (scientific journal), Joint Work

Perivascular nitric oxide gradients normalize tumor vasculature

Kashiwagi S., Tsukada K., Xu L., Miyazaki J., Kozin SV., Tyrrell JA, Sessa WC., Gerweck LE., Jain RK, Fukumura D.

Nature Medicine 14 ( 3 ) 255-257 2008

Research paper (scientific journal), Single Work, Accepted