Papers - Fujimoto, Keiji

Cell activation by the micro-patterned particle surface

Fujimoto,K., Takahashi,T., Miyaki,M., and Kawaguchi,H.

Journal of Biomaterials Science, Polymer Edition vol.8   879-891 1997

Joint Work, Accepted

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細胞が材料表面の微細な形態を認識し、その影響のもとに自分自身の形態や性質を変化さ せているといった報告が数多くなされるようになってきた。我々は表面に微粒子を規則的に並べた凸状の表面構造を作製し、細胞に与える影響について検討を行った。

Preparation and Structural Characteristics of Stimuli-Responsive Hydrogel Microsphere

Nakazawa,Y., Kamijo,Y., Fujimoto,K., Kawaguchi,H., Yuguchi,Y., Urakawa,H., and Kajiwara,K.

Die Angewandte Makromolekulare Chemie, (Angewandte Makromolekulare Chemie)  vol.240   187-196 - 196 1996

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00033146

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アクリルアミドとメタアクリル酸から刺激応答性ゲル微粒子を作製した。この微粒子はpHに応答したサイズ変化を示した。

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Selective Isolation of DNA or RNA Using Single-Stranded DNA Affinity Latex Particles

Imai,T., Sumi,Y., Hatakeyama,M., Fujimoto,K., Kawaguchi,H., Hayashida,N., Shiozaki,K., Terada, K., Yajima,H., and Handa,H.

Journal of Colloid and Interface Science (Journal of Colloid and Interface Science)  vol.177 ( 1 ) 245-249 - 249 1996

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00219797

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一本鎖のDNAを微粒子に固定化し、そのDNAと相補的なDNAあるいはRNAの単離を試みたところ、短時間で簡便に回収できることがわかった。

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Preparation and Structural Characterization of Hydrogel Microspheres

Kamijo,Y., Fujimoto,K., Kawaguchi,H., Yuguchi,Y., Urakawa,H., and Kajiwara,K.

Polymer Journal (Polymer Journal)  vol.28 ( 4 ) 309-316 - 316 1996

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00323896

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様々な溶媒を用いて沈殿重合によりゲル微粒子の作製を行った。ゲル微粒子の生成と溶媒の性質との関係を見いだし、さらに重合途中の微粒子の核形成の過程を検出することにより沈殿重合についての新しい知見を得ることが出来た。

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Modification and functionalization of hydrogel microspheres

Kawaguchi,H., Fujimoto,K., Nakazawa,Y., Sakagawa,M., Ariyoshi,Y., Shidara,M., Okazaki,H., and Ebisawa,Y.

Colloids and Surfaces, A: Physicochem. Eng. Aspects (Colloids and Surfaces A: Physicochemical and Engineering Aspects)  vol.109   147-154 - 154 1996

Research paper (scientific journal), Joint Work, Accepted,  ISSN  09277757

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沈殿重合により反応性ゲル微粒子を作製し、機能化を目指した様々な改質を行った。

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Chemical Properties of Water-Soluble, Nonionic Azo Compounds as Initiators for Emulsion Polymerization

Torii,H., Fujimoto,K., and Kawaguchi,H.

J. Polym. Sci., Polym. Chem. Ed. (Journal of Polymer Science, Part A: Polymer Chemistry)  vol.34 ( 7 ) 1237-1244 - 1243 1996

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0887624X

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ノニオン性の開始剤を用いた乳化重合を行った。この開始剤は水溶性であり、静電的な反発力に起因する分散安定化効果がなくとも、この水溶性に由来する立体反発力により微粒子の生成がなされたものと考えられた。

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Interactions between temperature‐sensitive hydrogel microspheres and granulocytes

Achiha K., Ojima R., Kasuya Y., Fujimoto K., Kawaguchi H.

Polymers for Advanced Technologies (Polymers for Advanced Technologies)  6 ( 7 ) 534 - 540 1995.07

Research paper (scientific journal), Accepted,  ISSN  10427147

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Poly(N‐isopropylacrylamide) (PNIPAM) has a low critical solution temperature (LCST) at 32°C in water and the hydrophilicity changes through the LCST. The microspheres whose surface was composed of PNIPAM exhibited phase transition behavior around 32°C. Therefore, the interactions between PNIPAM micropheres and granulocytes depended on the temperature. That is, the oxygen consumption and active oxygen production by cells in contact with PNIPAM‐containing microspheres and adhesion of the microspheres to the cell surface were more enhanced above the LCST of PNIPAM than below it, whereas no significant temperature dependence of cell–microspheres interaction was observed in nonthermosensitive microsphere systems. It was suggested that the function of cells could be controlled with temperature using the temperature‐sensitive microspheres. Copyright © 1995 John Wiley & Sons, Ltd.

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Enzyme immobilization on thermosensitive hydrogel microspheres

Shiroya,T., Tamura,N., Yasui,M., Fujimoto,K., and Kawaguchi,H.

Colloids and Surfaces B: Biointerfaces (Colloids and Surfaces B: Biointerfaces)  vol.4 ( 5 ) 267-274 - 274 1995

Research paper (scientific journal), Joint Work, Accepted,  ISSN  09277765

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感温性ゲル微粒子に酵素を固定化し、温度変化による酵素活性の制御を行った。

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Control of enzymatic activity using thermosensitive polymers

Shiroya,T., Yasui,M., Fujimoto,K., and Kawaguchi,H.

Colloids and Surfaces B: Biointerfaces (Colloids and Surfaces B: Biointerfaces)  vol.4 ( 5 ) 275-285 - 285 1995

Research paper (scientific journal), Joint Work, Accepted,  ISSN  09277765

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感温性ポリマー鎖に酵素を固定化し、温度変化による酵素活性の制御を行った。

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Preparation of monodisperse, reactive hydrogel microspheres and their amphoterization

Kashiwabara,M., Fujimoto,K., and Kawaguchi,H.

Colloid & Polymer Science (Colloid and Polymer Science)  vol.273 ( 4 ) 339-345 - 345 1995

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0303402X

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沈殿重合により粒子径が均一で、反応性部位を有する微粒子を作製した。その反応性部位を介して微粒子の両性化を行った。

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Interactions between temperature-sensitive hydrogel microspheres and granulocytes

Achiha,K., Ojima,R., Kasuya,Y., Fujimoto,K., and Kawaguchi,H.

Polymers for Advanced Technologies vol.6   534-540 1995

Joint Work, Accepted

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感温性ゲル微粒子と顆粒球との相互作用を調べた。低温で水和状態の微粒子は顆粒球に付着すらしなかった。しかし、温度を上げて脱水和させると微粒子は顆粒球に付着するようになったが、貪食されることはなかった。

Purification of membrane receptors with peptide-carrying affinity latex particles

Inomata,Y., Kasuya,Y., Fujimoto,K., Handa,H., and Kawaguchi,H.

Colloids and Surfaces B: Biointerfaces (Colloids and Surfaces B: Biointerfaces)  vol.4 ( 4 ) 231-241 - 241 1995

Research paper (scientific journal), Joint Work, Accepted,  ISSN  09277765

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膜レセプターと特異的に接着するペプチド断片を微粒子に固定化した。その微粒子を用いて顆粒球の膜レセプターの分離に成功した。

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Purification of transcription factor 2A using yeast TATA-binding protein affinity latex particles

Shiroya,T., Hatakeyama,M., Fujimoto,K., Watanabe,H., Wada,T., Imai,T., Kawaguchi,H., and Handa,H.

IJBC vol.1   191-198 1995

Joint Work, Accepted

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DNA鎖のTATA配列部分を微粒子に固定化することにより転写制御因子TF2Aの分離精製を試みた。この微粒子によるバッチシステムは、非特異的な成分の吸着が非常に少なく、短時間で高効率の分離が可能であった。

Cytoplasmic calcium level and membrane fluidity of platelets contacting poly(acrylamide-co-methacrylic acid) particles with different surface properties

Yui,N., Suzuki,K., Okano,T., Sakurai,Y., Nakano,M., Ishikawa,C., Fujimoto,K., and Kawaguchi,H.

Journal of Biomaterials Science, Polymer Edition (Journal of Biomaterials Science, Polymer Edition)  vol.7 ( 3 ) 253-264 - 264 1995

Research paper (scientific journal), Joint Work, Accepted,  ISSN  09205063

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様々な組成比を有するアクリルアミドとメタクリル酸の共重合体からなる微粒子を作製した。これらの微粒子が血小板に与える作用を細胞内カルシウム濃度と膜の流動性の観点から検討を行った。

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Effects of surface modification of materials on human neutrophil activation

Wakabayashi,Y., Sasaki,J., Fujita,H., Fujimoto,K., Morita,I., Murota,S., and Kawaguchi,H.

Biochimica et Biophysica Acta (BBA - General Subjects)  vol.1243 ( 3 ) 521-528 - 528 1995

Research paper (scientific journal), Joint Work, Accepted,  ISSN  03044165

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好中球の非特異的な活性化についての知見を得るために、ポリスチレン表面の表面化学改質を行った。その表面と接触した細胞の活性酸素の放出やレセプター類の発現を調べた。

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Dipyridamole, an anti-platelet agent, reduces the vascular endothelial cell injury induced by active oxygen species

Wakabayashi Y., Fujita H., Fujimoto K., Kawaguchi H., Morita I., Murota S.

Platelets (Platelets)  6 ( 3 ) 176 - 182 1995

Research paper (scientific journal), Accepted,  ISSN  09537104

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The effects of the anti-platelet agent dipyridamole on vascular endothelial cells were assessed by measurement of the injury index induced by oxygen stress. Vascular endothelial cell injury was assayed by measuring 51 Cr release from labeled vascular endothelial cells. Leukocytes activated by phorbol 12-myristate 13-acetate (PMA) were used to induce the injury of endothelial cells. In this system, dipyridamole suppressed endothelial cell injury in a dose dependent manner (0.1-10 4mUM) while it had no effect on production of superoxide anion in PMA-activated leukocytes. Treatment of endothelial cells with hydrogen peroxide also induced endothelial cell injury in a dose dependent manner (50-150 μM). Dipyridamole also prevented the endothelial cell injury induced by hydrogen peroxide with a dose dependent fashion (1-10 μM). There were no significant changes in the activities of catalyzing enzymes such as catalase and glutathione peroxidase in the endothelial cells following dipyridamole treatment. In contrast, dipyridamole significantly increased the cyclic GMP content of endothelial cells in a dose dependent manner (1-10 μM). Addition of 8-bromo-cyclic GMP (1 mM) to the culture also protected endothelial cells from injury induced by hydrogen peroxide, but 8-bromo-cyclic AMP did not. These data suggest that the protective effect of dipyridamole against oxygen stress is correlated with the increase in the cyclic GMP content of the endothelial cells. © 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

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Purification of transcription factor IIA using yeast TATA-binding protein affinity latex particles

Shiroya T., Hatakeyama M., Fujimoto K., Watanabe H., Wada T., Imai T., Kawaguchi H., Handa H.

International Journal of Bio-Chromatography (International Journal of Bio-Chromatography)  1 ( 3 ) 191 - 198 1995

Research paper (scientific journal), Accepted,  ISSN  10680659

Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres

Kasuya Y., Fujimoto K., Kawaguchi H., Miyamoto M.

Biomaterials (Biomaterials)  15 ( 8 ) 570 - 576 1994.06

Research paper (scientific journal), Accepted,  ISSN  01429612

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A cell adhesive peptide, Arg-Gly-Asp-Ser (RGDS), enhances leucocyte response to stimuli when insolubilized or conjugated with proteins. In order to investigate the contribution of the microsphere structure to leucocyte response, RGDS was immobilized onto several polymeric microspheres. The respiratory burst of polymorphonuclear leucocytes (PMNs) contacting microspheres was enhanced by RGDS in cases where it was immobilized on polystyrene and, more effectively, a positively charged one. This was not observed in polyacrylamide-based or negative-charged microsphere systems. These results demonstrate that the property of supports strongly affects the regulation of the PMN response via an adhesive interaction. © 1994.

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Activation of human neutrophils by Arg‐Gly‐Asp‐Ser immobilized on microspheres

Kasuya Y., Fujimoto K., Miyamoto M., Kawaguchi H.

Journal of Biomedical Materials Research (Journal of Biomedical Materials Research)  28 ( 3 ) 397 - 404 1994.03

Research paper (scientific journal), Accepted,  ISSN  00219304

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The adhesive interaction of cells with extracellular matrix components is essential for a variety of cellular functions, and is frequently mediated by a tetra peptide, Arg‐Gly‐Asp‐Ser (RGDS), located within fibronectin and other proteins. In this study, the RGDS‐mediated activation of polymorphonuclear leukocytes accompanied by phagocytosis was investigated using monodisperse polymeric microspheres carrying RGDS. The parent and Arg‐Gly‐Glu‐Ser (RGES)‐carrying microspheres, which have no adhesion activity, were employed as controls. The ingestion of microspheres into PMN was not enhanced by immobilizing RGDS. However, PMNs exhibited unique oxygen consumption and enhanced liberation of reactive oxygen when RGDS‐carrying microspheres were phagocytosed. These PMN responses disappeared with the addition of soluble RGDS. Furthermore, cytochalasin D, which inhibits actin polymerization, showed a marked inhibitory effect on oxygen consumption in the RGDS‐carrying microsphere system, as compared with those in other systems. These findings show that RGDS‐carrying microspheres induced the biospecific activation of PMNs by the signal transduction via RGDS‐integrin binding without alteration in the degree of phagocytosis. © 1994 John Wiley & Sons, Inc. Copyright © 1994 John Wiley & Sons, Inc.

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Surface structure of latex particles covered with temperature-sensitive hydrogel layers

Makino,K., Yamamoto,S., Fujimoto,K., Kawaguchi,H., and Ohshima,H.

Journal of Colloid and Interface Science (Journal of Colloid And Interface Science)  vol.166 ( 1 ) 251-258 - 258 1994

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00219797

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コア部に電荷を持ち、感温性のヘアを表面に有するコアシェル微粒子の泳動挙動を測定し、電荷導入によるヘア層の状態変化について検討を行った。

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