Nakazawa, Hideo

写真a

Affiliation

School of Medicine Department of biology (Hiyoshi)

Position

Research Associate/Assistant Professor/Instructor

External Links
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Career 【 Display / hide 】

  • 2000.04
    -
    2001.03

    早稲田大学　客員研究員

  • 2001.04
    -
    2003.03

    法政大学他　非常勤講師

  • 2003.04
    -
    2007.03

    医学部生物学教室, 助手

  • 2007.04
    -
    Present

    医学部生物学教室, 助教

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Academic Background 【 Display / hide 】

  • 1995.03

    Waseda University, School of Education, Department of Biology

    University, Graduated

  • 1997.03

    Waseda University, Graduate School of Science and Engineering, Department of Pure and Applied Physics

    Graduate School, Completed, Master's course

  • 2000.03

    Waseda University, Graduate School of Science and Engineering, Department of Pure and Applied Physics

    Graduate School, Completed, Doctoral course

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Academic Degrees 【 Display / hide 】

  • Ph.D., Waseda University, 2000.03

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Books 【 Display / hide 】

  • 生き物と匂い・フェロモンの事典

    東原　和成　他 編集, 朝倉書店, 2025.06

    Scope: 第2章　嗅覚受容メカニズム　2.1.2　両生類（イモリ，カエル）,  Contact page： 16-19 , Accepted

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Papers 【 Display / hide 】

  • Subdivisions of the olfactory epithelium in Japanese toads with distinct cell types and odor responsiveness.

    NAKAZAWA HIDEO, Atsushi C. Suzuki, Takatoshi Nagai

    The Japanese Journal of Taste and Smell Research (The Japanese Association for the Study of Taste and Smell)  21 ( 3 ) 425 - 428 2014.12

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  1340-4806

  • Involvement of Gαolf-expressing neurons in the vomeronasal system of Bufo japonicus

    Hagino-Yamagishi Kimiko, NAKAZAWA HIDEO

    The Journal of Comparative Neurology (New York, Wiley-Liss)  519 ( 16 ) 3189-3201 - 3201 2011.11

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  0021-9967

    　View Summary

    Most terrestrial vertebrates possess anatomically distinct olfactory organs: the olfactory epithelium (OE) and the vomeronasal organ (VNO). In rodents, olfactory receptors coupled to G alpha(olf) are expressed in the OE, whereas vomeronasal receptors type 1 (V1R) and vomeronasal receptors type 2 (V2R), coupled to G alpha(i2) and G alpha(o), respectively, are expressed in the VNO. These receptors and G proteins are thought to play important roles in olfactory perception. However, we previously reported that only V2R and G alpha(o) expression is detected in the Xenopus laevis VNO. As X. laevis spends its entire life in water, we considered that expression of limited types of chemosensory machinery in the VNO might be due to adaptation of the VNO to aquatic life. Thus, we analyzed the expression of G proteins in the VNO and the accessory olfactory bulb (AOB) of the adult Japanese toad, Bufo japonicus, because this species is well adapted to a terrestrial life. By using immunohistochemical analysis in combination with in situ hybridization and Dil labeling, we found that B. japonicus G alpha(olf) and G alpha(o) were expressed in the apical and middle-to-basal layer of the vomeronasal neuroepithelium, and that the axons of these G alpha(olf)- and G alpha(o)-expressing vomeronasal neurons projected to the rostral and caudal accessory olfactory bulb, respectively. These results strongly suggest that both the G alpha(olf)- and G alpha(o)-mediated signal transduction pathways function in the B. japonicus VNO. The expression of G alpha(olf) in the B. japonicus VNO may correlate with the detection of airborne chemical cues and with a terrestrial life. J. Comp. Neurol. 519:3189-3201, 2011. (C) 2011 Wiley- Liss, Inc.

  • Seasonal Increase in Olfactory Receptor Neurons of the Japanese Toad, Bufo japonicus, is Paralleled by an Increase in Olfactory Sensitivity to Isoamyl Acetate

    Hideo Nakazawa, Masumi Ichikawa and Takatoshi Nagai

    Chemical Senses (Oxford University Press)  34 ( 8 ) 667-678 - 678 2009.07

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  0379-864X

    　View Summary

    Japanese toads (Bufo japonicus) migrate to and from breeding sites in the early spring, possibly guided by olfactory cues. We previously showed that the electrical activity of olfactory receptor neurons (ORNs) in the toads was enhanced in the breeding period. We undertook morphological and physiological studies of the olfactory epithelium to determine whether any cellular substrate of the epithelium underlies the enhanced electrical activity of ORNs. The ORNs of the toads were labeled by antiserum to olfactory marker protein (OMP), and the morphology of the labeled cells and their distribution in the epithelium were examined throughout the year. The OMP-positive cells, distributed mainly in the basal and intermediate layers of the epithelium, were most numerous in the early breeding period. Cell proliferation in the epithelium detected by 5-bromo-2'-deoxyuridine labeling was most elevated in this period. The electrical activity of ORNs was examined by recording the electroolfactogram (EOG) in the toads throughout the year. Statistical analysis showed a positive correlation between the density of OMP-positive cells in the epithelium and the amplitude of the EOG responses. A greater number of ORNs in the breeding period possibly aids the toads in migrating to their breeding sites.

  • Axonal Projection of OMP-Immunoreactive Cells in the Anterior Sac of Choana in Tadpoles of the Japanese Toad (Bufo japonicus)

    NAKAZAWA HIDEO HIRANO Akinori, ICHIKAWA Masumi NAGAI Takatoshi

    Chemical Senses (Oxford University Press)  32 ( 2 ) J25 - J25 2007.02

    Research paper (scientific journal), Single Work, Accepted,  ISSN  0379-864X

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Fine structure and function of the recessus olfactorius, a sensory epithelium of the accessory olfactory system, in the toad (Bufo japonicu)

    2011.04
    -
    2014.03

    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research, NAKAZAWA Hideo, Grant-in-Aid for Young Scientists (B), Research grant, Principal investigator

    　View Summary

    The recessus olfactorius (RO) is a small pouch observed in the antero-lateral olfactory epithelium of anuran amphibians. In Japanese toads the central projection from the epithelium lining RO (ROE) was observed by using fluorescent carbocyanine dye, and it revealed that the neuronal fibers from the ROE did not project to the main olfactory bulb (MOB) but to the accessory olfactory bulb (AOB). The projections from the ROE were found in the anterior region of the AOB and also in the anterior half of the posterior region. In electron microscopy the tissue collected from the ROE was compared with those from the vomeronasal epithelium (VNE) and the olfactory epithelium (OE). The ROE and the VNE contained both ciliated cells and microvillous cells. The odor response of ROE was measured by using voltage sensitive dye (di-8ANEPPS). The ROE showed responses to airborne and waterborne odorants, such as cineole and amino acids.

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Courses Taught 【 Display / hide 】

  • LABORATORY OF BIOLOGY

    2025

  • LABORATORY OF BIOLOGY

    2024

  • LABORATORY OF BIOLOGY

    2023

  • LABORATORY OF BIOLOGY

    2022

  • LABORATORY OF BIOLOGY

    2021

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Courses Previously Taught 【 Display / hide 】

  • 生物学実験

    Keio University

    2018.04
    -
    2019.03

  • LABORATORY OF BIOLOGY

    Keio University

    2017.04
    -
    2018.03
    , 

    Full academic year, Laboratory work/practical work/exercise

  • BIOLOGY2

    Keio University

    2017.04
    -
    2018.03
    , 

    Spring Semester, Lecture

  • 生物学実験

    生物学教室

    2016.04
    -
    2017.03
    , 

    Full academic year, Laboratory work/practical work/exercise

  • 生物学実験

    生物学教室

    2015.04
    -
    2016.03
    , 

    Full academic year, Laboratory work/practical work/exercise

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