Details of a Researcher - Hasegawa, Naoki

Disseminated Mycobacterium massiliense infection in a patient with myelodysplastic syndrome undergoing allogeneic bone marrow transplantation

Toyama T., Mori T., Kato J., Sugita K., Hasegawa N., Nakata N., Hoshino Y., Okamoto S.

Transplant Infectious Disease （Transplant Infectious Disease) 22 （ 3 ） 2020.06

ISSN 13982273

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© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Nontuberculous mycobacteria are ubiquitous in water and soil, and the subset of rapidly growing mycobacteria species can cause severe infections in immunocompromised patients. Solid organ or hematopoietic stem cell transplantation (HSCT) recipients are known to be susceptible to infection by nontuberculous mycobacteria. The nontuberculous mycobacteria species Mycobacterium massiliense (M massiliense) has been classified as a rapidly growing mycobacteria and recognized as a pathogen causing lung and soft tissue infections in humans. However, there have been only a few reported cases of M massiliense infection after solid organ transplantation and HSCT. We herein report another case of M massiliense infection after allogeneic HSCT, which manifested as soft tissue infection, lung infection, and bacteremia.
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ADAM17 protects against elastase-induced emphysema by suppressing CD62L+ leukocyte infiltration in mice

Suzuki S., Ishii M., Asakura T., Namkoong H., Okamori S., Yagi K., Kamata H., Kusumoto T., Kagawa S., Hegab A.E., Yoda M., Horiuchi K., Hasegawa N., Betsuyaku T.

American Journal of Physiology - Lung Cellular and Molecular Physiology （American Journal of Physiology - Lung Cellular and Molecular Physiology) 318 （ 6 ） L1172 - L1182 2020.06

ISSN 10400605

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© 2020 the American Physiological Society Pulmonary emphysema is a major manifestation of chronic obstructive pulmonary disease and is associated with chronic pulmonary inflammation caused by cigarette smoking, with contributions from immune cells such as neutrophils, macrophages, and lymphocytes. Although matrix metalloproteinases are well known to contribute to emphysema progression, the role of a disintegrin and metalloproteinase (ADAM) family proteins, other major metalloproteinases, in disease pathogenesis is largely unknown. ADAM17 is a major sheddase that cleaves various cell surface proteins, including CD62L, an adhesion molecule that plays a critical role in promoting the migration of immune cells to the site of inflammation. In the present study, we aimed to investigate the potential role of ADAM17 and CD62L in the development of elastase-induced emphysema. Control and Adam17flox/flox/Mx1-Cre(Adam17ΔMx1) mice (8-10 wk old) were intratracheally injected with 5 units of porcine pancreas elastase and monitored for 35 days after injection. Lung alveolar destruction was evaluated by analyzing the mean linear intercepts of lung tissue specimens and by histopathological examination. Mean linear intercepts data indicated that the degree of elastase-induced emphysema was significantly more severe in Adam17ΔMx1 mice. Furthermore, flow cytometry showed that CD62L+ neutrophil, CD62L+ macrophage, and CD62L+ B lymphocyte numbers were significantly increased in Adam17ΔMx1 mice. Moreover, the pharmacological depletion of CD62L+ cells with a CD62L-neutralizing antibody ameliorated the extent of emphysema in Adam17ΔMx1 mice. Collectively, these results suggest that ADAM17 possibly suppresses the progression of emphysema by proteolytically processing CD62L in immune cells and that ADAM17 and CD62L could be novel therapeutic targets for treating pulmonary emphysema.
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Seasonal changes in indoor airborne fungal concentration in a hematology ward

Mori T., Kikuchi T., Kato J., Koda Y., Sakurai M., Kikumi O., Inose R., Murata M., Hasegawa N., Nakayama H., Yamazaki R., Okamoto S.

Journal of Infection and Chemotherapy （Journal of Infection and Chemotherapy) 26 （ 4 ） 363 - 366 2020.04

ISSN 1341321X

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© 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases Invasive fungal disease (IFD) is an important infectious complication of hematological disorders, especially in hematopoietic stem cell transplantation recipients. Evidences suggest seasonal and/or geographical variations in the airborne fungal counts and a relationship between those counts and the incidence of IFD. We evaluated the concentrations of indoor airborne fungi quantitated over the course of one year in a hematology ward in Japan. In January, April, July, and October, fixed volumes of air samples were obtained by an air sampler in a hematology ward not equipped with a high-efficiency particulate air filter and incubated in fugal cultures. Samples were also obtained from a protective environment in the same ward and were evaluated. The number of fungal colonies per 50 L of sampled air was highest in October (median 2.25 (range, 0.2–7.0)), which was significantly higher than those in the other three months (0.1 (range, 0–1.0) in January; 0 (0-0) in April; 0.55 (0–2.5) in July; P < 0.01)). Commonly identified pathogens included Penicillium and Cladosrporium species, but Aspergillus species was detected only in July and October samples. These results suggest a seasonal variation in indoor airborne fungal concentrations in Japan, which could affect the epidemiology of IFD.
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Showering is associated with Mycobacterium avium complex lung disease: An observational study in Japanese women

Uwamino Y., Nishimura T., Sato Y., Tamizu E., Uno S., Mori M., Fujiwara H., Kawabe H., Murata M., Hasegawa N.

Journal of Infection and Chemotherapy （Journal of Infection and Chemotherapy) 26 （ 3 ） 211 - 214 2020.03

ISSN 1341321X

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© 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases Mycobacterium avium complex refers to a group of environmental bacteria which inhabit water and soil. Although Mycobacterium avium complex is capable of causing refractory lung infections, the risk factors for Mycobacterium avium complex lung disease are still unclear. This study aimed to determine the associations between Mycobacterium avium complex lung disease and soil or water exposure. Using questionnaires along with clinical data, we compared soil exposure, along with bathtub bathing and showering habits between 172 women with Mycobacterium avium complex lung disease and 339 women without Mycobacterium avium complex infection as controls. Showering was found to be independently associated with the presence of Mycobacterium avium complex lung disease (adjusted odds ratio 5.72, 95%, confidence interval 1.99 to 16.46). Although the mean age of the groups was different, an age-matched sub-analysis yielded similar results. These results indicate that showering may be a risk factor for Mycobacterium avium complex lung disease.
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Effect of refrigeration of blood samples in lithium-heparin tubes on QuantiFERON TB Gold Plus test result

Uwamino Y., Sakai A., Nishimura T., Noguchi M., Uno S., Fujiwara H., Mori M., Wakui M., Murata M., Hasegawa N.

Journal of Infection and Chemotherapy （Journal of Infection and Chemotherapy) 26 （ 3 ） 312 - 314 2020.03

ISSN 1341321X

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© 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases The QuantiFERON TB Gold Plus (QFT-Plus) test is a newly approved interferon-gamma releasing assay test for detecting latent tuberculosis. Although blood samples for QFT test can be refrigerated for 48 h in lithium-heparin tubes according to package inserts, no published data are available on the effects of sample refrigeration on the test results. We conducted a clinical study that aimed to elucidate whether sample refrigeration for 48 h affects QFT-Plus test results. We collected 2 blood samples each from 40 participants for QFT-Plus; one sample was refrigerated before incubation for QFT-Plus assay, while the other sample was incubated soon after collection and treated as control. After comparing QFT-Plus test results of refrigerated samples and control samples, the concordance rate and kappa coefficient between them were 95% and 0.90, respectively. Thus, blood samples for QFT-Plus test can be refrigerated for 48 h in lithium-heparin tubes without influencing the test results.
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Papers, etc., Registered in KOARA 【 Display / hide 】

Elucidation of the host immune response to nontuberculous mycobacterial cell wall lipids

Hasegawa, Naoki

科学研究費補助金研究成果報告書 2018
Elucidation of miRNA-mediated pathophysiology of MAC pulmonary disease

Hasegawa, Naoki

科学研究費補助金研究成果報告書 2018
Association of sex steroid hormones and non-tuberculous mycobacteriosis

Hasegawa, Naoki

科学研究費補助金研究成果報告書 2017
The development of the measuring assay for anti- HIV drug concentration of hair as the evaluation method of medication compliance

Hasegawa, Naoki

科学研究費補助金研究成果報告書 2015
An analysis of miRNA related host-microbial crosstalk

Hasegawa, Naoki

科学研究費補助金研究成果報告書 2015

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Reviews, Commentaries, etc. 【 Display / hide 】

Editorial Comment to Comparison of single- and multiple-dose cefazolin as prophylaxis for transurethral enucleation of prostate: A multicenter, prospective, randomized controlled trial by the Japanese Research Group for Urinary Tract Infection

Yazawa S., Hasegawa N., Oya M.

International Journal of Urology (International Journal of Urology) 27 ( 3 ) 248 - 249 2020.03

ISSN 09198172
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Correction: Low serum estradiol levels are related to Mycobacterium avium complex lung disease: A cross-sectional study (BMC Infectious Diseases (2019) 19 (1055) DOI: 10.1186/s12879-019-4668-x)

Uwamino Y., Nishimura T., Sato Y., Tamizu E., Asakura T., Uno S., Mori M., Fujiwara H., Ishii M., Kawabe H., Murata M., Hasegawa N.

BMC Infectious Diseases (BMC Infectious Diseases) 20 ( 1 ) 2020.02

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© The Author(s). 2020. After publication of the original article [1], we were notified that units of testosterone in main text and abstract and units of DHEA-S in Fig. 1 and Table 4 are incorrect. 1. In the Abstract: 'testosterone (0.230 ng/L vs. 0.250 ng/L, p = 0.005)' should be replaced with 'testosterone (0.230 ng/mL vs. 0.250 ng/mL, p = 0.005)' 2. In the section 'Serum levels of sex hormones': 'testosterone (0.23 ng/L vs. 0.25 ng/L, p = 0.005)' should be replaced with 'testosterone (0.23 ng/mL vs. 0.25 ng/mL, p = 0.005)' 3. The unit of serum DHEA-S, in Fig. 1 (3rd column): 'μg/mL' should be replaced with 'μg/dL' 4. unit of DHEA-S in Table 4 (4th row): 'μg/mL' should be replaced with 'μg/dL'.
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Recovery of FRI-5 carbapenemase at a Japanese hospital where FRI-4 carbapenemase was discovered

Uwamino Y., Kubota H., Sasaki T., Kosaka A., Furuhashi M., Uno S., Kudoh J., Murata M., Hasegawa N.

Journal of Antimicrobial Chemotherapy (Journal of Antimicrobial Chemotherapy) 74 ( 11 ) 3390 - 3392 2019.11

ISSN 03057453
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From the authors

Kwak N., Dalcolmo M.P., Daley C.L., Eather G., Hasegawa N., Koh W.J., Thomson R., Van Ingen J., Yim J.J.

European Respiratory Journal (European Respiratory Journal) 54 ( 5 ) 2019.11

ISSN 09031936
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Mycobacterium triplex pulmonary disease with acquired macrolide resistance in immunocompetent patients

Matsuda S., Suzuki S., Morimoto K., Aono A., Nishio K., Asakura T., Sasaki Y., Namkoong H., Nishimura T., Ogata H., Hasegawa N., Kurashima A., Ishii M., Tatsumi K., Mitarai S., Goto H.

Clinical Microbiology and Infection (Clinical Microbiology and Infection) 24 ( 6 ) 671 - 672 2018.06

ISSN 1198743X
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Presentations 【 Display / hide 】

肺非定型抗酸菌症（MAC）症の治療例における血清KL-6およびSPD値の経時的変化

YAMAGUCHI KAZUHIRO

第44回日本呼吸器学会総会 (東京) ,

2004.03
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Poster presentation
エンドトキシン気道内投与のシグナル伝達に対する抗CD14抗体とtoll-like receptor4遺伝子変異の影響

YAMAGUCHI KAZUHIRO

第44回日本呼吸器学会総会 (東京) ,

2004.03
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Oral presentation (general)
エンドトキシン（LPS）投与マウス肺損傷モデルにおける抗VEGF抗体投与の効果

YAMAGUCHI KAZUHIRO

第44回日本呼吸器学会総会 (東京) ,

2004.03
,
Poster presentation
日本人軽症喘息患者におけるロイコトリエン受容体拮抗薬反応性規定因子の解析

YAMAGUCHI KAZUHIRO

第44回日本呼吸器学会総会 (東京) ,

2004.03
,
Poster presentation
エンドトキシン（LPS）気道内注入肺損傷モデルにおけるTNF-α Converting Enzyme（TACE）阻害薬の効果

YAMAGUCHI KAZUHIRO

第44回日本呼吸器学会総会 (東京) ,

2004.03
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Poster presentation

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

Elucidation of pathogenesis of pulmonary diseases due to nontuberculous mycobacteria using the human lungs

2022.04

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2025.03

MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator
Investigation on pathogenesis of pulmonary diseases due to nontuberculous mycobacteria using human genomic analysis

2019.04

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2022.03

MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator
miRNAを介した肺MAC症の病態解明

2016.04

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2019.03

MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator