Papers - Wakui, Masatoshi

Assessment of in vitro effects of direct thrombin inhibitors and activated factor X inhibitors through clot waveform analysis

Wakui M, Fujimori Y, Katagiri H, Nakamura S, Kondo Y, Kuroda Y, Nakagawa T, Shimizu N, Murata M

J Clin Pathol (Journal of Clinical Pathology)  72 ( 3 ) 244 - 250 2019.03

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00219746

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© Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ. Aims Clot waveform analysis (CWA) has been reported to extend the interpretation of clotting time measurement. The parameters obtained from successive derivatives of the clotting reaction curves reflect the rates of activation of individual coagulation factors, theoretically dissecting the cascade pathway. This study aims to assess the in vitro effects of direct thrombin inhibitors (DTIs) and activated factor X (FXa) inhibitors. Methods CWA was applied to the activated partial thromboplastin time (APTT) assay of plasma samples spiked with each drug. For CWA of APTT measurement curves (APTT-CWA), the positive mode of clotting reaction curves was defined as the direction towards fibrin generation. Results All the maximum positive values in the successive derivatives were decreased dependently on the concentrations of each drug. Moreover, the negative values in the second and third derivatives appeared putatively due to consumption of thrombin and factor FXa, respectively, to form complexes with plasma serine protease inhibitors. The decrease of the maximum negative values observed dependently on the concentrations of each drug appeared to be consistent with the decreased generation of thrombin and factor FXa. The analysis of Hill coefficients of each drug in the dose-response of changes in the APTT-CWA parameters revealed a difference in anticoagulant cooperativity between DTIs versus FXa inhibitors. Conclusions The APTT-CWA demonstrated evidence for the blockade of thrombin-positive feedback by DTIs and FXa inhibitors and that for the differences in anticoagulant cooperativity between them. The results demonstrate the usability of CWA for assessment of anticoagulation and provide insights into direct anticoagulants.

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Fatty acid β-oxidation dependent and independent responses and tumor aggressiveness acquired under mild hypoxia

Wakui M, Kawai K, Mizushima T, Nishime C, Serizawa A, Suemizu H, Asakura K, Yamauchi Y, Hayashida T, Suematsu M, Murata M

Anticancer Res (Anticancer Research)  39 ( 1 ) 191 - 200 2019.01

Research paper (scientific journal), Joint Work, Accepted,  ISSN  02507005

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© 2019 International Institute of Anticancer Research. All Rights Reserved. Background/Aim: The present study assessed whether and how tumor cells undergoing hypoxia contribute to disease progression after moving to areas with different oxygen conditions. Materials and Methods: Human colorectal carcinoma HCT116 cells cultured under mild hypoxia were subjected to in vivo experiments using transfer to immunodeficient murine recipients and to in vitro experiments using pharmacological inhibition of fatty acid β-oxidation (FAO). Results: Bone involvement and hepatic metastases were accelerated in transfer models of hypoxically cultured HCT116 cells. Hypoxic HCT116 cells exhibited FAO-dependent glycogen synthesis. FAO-dependent and -independent induction of gene expression also occurred under hypoxia. The distribution of glucose transporter 1 expression compared with heme oxygenase 1 expression in HCT116 cell spheroids seemed consistent with differential dependence of hypoxic expression of these molecules on FAO. Conclusion: These results provide insights into the contribution of hypoxia to tumor progression and the relevance of FAO.

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Methodology in a new era for clinical laboratories – Potential and pitfalls of mass spectrometer

Terumichi Nakagawa, Masatoshi Wakui, MD, PhD

Rinsho Byori (the official journal of Japanese Society of Laboratory Medicine) (Japanese Society of Laboratory Medicine)  66 ( 4 ) 398 - 407 2018.04

Research paper (scientific journal), Joint Work

Increased levels of prostaglandin E-major urinary metabolite (PGE-MUM) in active mesenteric panniculitis patients: A case report

Mizuno S, Wakui M, Machida Y, Hosoe N, Hisamatsu T, Ishida T, Kameyama K, Naganuma M, Kanai T

Medicine 96 ( 51 ) e9237 - e9240 2017.12

Research paper (scientific journal), Joint Work, Accepted

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Understanding of coagulation tests from the perspective of chemistry

Masatoshi Wakui, Yuta Fujimori, Hisako Katagiri

Japanese Journal of Clinical Chemistry (日本臨床化学会)  46 ( 2 ) 95 - 104 2017

Research paper (scientific journal), Joint Work

Validation of chemiluminescent enzyme immunoassay in detection of autoantibodies in pemphigus and pemphigoid

Fujio Y, Kojima K, Hashiguchi M, Wakui M, Murata M, Amagai M, Yamagami J

J Dermatol Sci 85 ( 3 ) 208 - 215 2017

Research paper (scientific journal), Joint Work, Accepted

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Potential utility and limitations of prothrombin time (PT), activated partial thromboplastin time (APTT), and anti-Xa assays for monitoring of Xa inhibitors

Ohira K, Fujimori Y, Katagiri H, Shimizu N, Mitsuhashi T, Wakui M, Murata M

Journal of The Japanese Society for Laboratory Hematology (日本検査血液学会)  18 ( 1 ) 24 - 29 2017

Research paper (scientific journal), Joint Work, Accepted

免疫学的測定法の不正確さと質量分析法の落とし穴

WAKUI Masatoshi

臨床化学 (日本臨床化学会)  45 ( 4 ) 369 - 370 2016.12

Research paper (scientific journal), Joint Work, Accepted

Potential application of fibrinogen measurement based on the Clauss assay to monitoring of dabigatran

Fujimori Y, Wakui M, Katagiri H, Ohira K, Shimizu N, Mitsuhashi T, Murata M

Rinsho Byori (日本臨床検査医学会)  64 ( 7 ) 765 - 770 2016.07

Research paper (scientific journal), Joint Work, Accepted

Evaluation of anticoagulant effects of direct thrombin inhibitors, dabigatran and argatroban, based on the Lineweaver-Burk plot applied to the Clauss assay

Fujimori Y, Wakui M*#, Katagiri H, Ohira K, Shimizu N, Murata M

J Clin Pathol 69 ( 4 ) 370 - 372 2016.04

Research paper (scientific journal), Joint Work, Accepted

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アデノシンデアミナーゼ.

WAKUI Masatoshi

medicina 52 ( 4 ) 226 - 227 2015

Research paper (scientific journal), Single Work

Innate Response to Human Cancer Cells with or without IL-2 Receptor Common γ-Chain Function in the NOD Background Mice Lacking Adaptive Immunity.

Nishime C, Kawai K, Yamamoto T, Katano I, Monnai M, Goda N, Mizushima T, Suemizu H, Nakamura M, Murata M, Suematsu M, Wakui M*.

J. Immunol. 195 ( 4 ) 1883 - 1890 2015

Research paper (scientific journal), Joint Work, Accepted

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Basis examination of direct thrombin inhibitor monitoring using two fibrinogen reagents containing different thrombin unit

Fujimori Y, Katagiri H, Wakui M, Shimizu N, Sakata T, Mitsuhashi T, Murata M

Journal of The Japanese Society for Laboratory Hematology 16 ( 3 ) 247 - 252 2015

Research paper (scientific journal), Joint Work, Accepted

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平成28年度日本検査血液学会学術賞受賞論文
https://www.keio.ac.jp/ja/research/researchers/award.html

酸ホスファターゼ.

WAKUI Masatoshi

medicina 52 ( 4 ) 228 - 230 2015

Research paper (scientific journal), Single Work

Bevacizumab terminates homeobox B9-induced tumor proliferation by silencing microenvironmental communication.

Hoshino Y, Hayashida T, Hirata A, Takahashi H, Chiba N, Ohmura M, Wakui M, Jinno H, Hasegawa H, Maheswaran S, Suematsu M, Kitagawa Y.

Mol Cancer 13 ( 1 ) 102 2014

Research paper (scientific journal), Joint Work, Accepted

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Discordance between Two Interferon-Gamma Release Assays in the Diagnosis of Latent Tuberculosis Infection in Healthcare Workers

Fujiwara H, Nishimura T, Iketani O, Takano Y, Sakai A, Kondo N, Ohtake K, Oguchi S, Shimizu N, Shibata A, Wakui M, Murata M, Mori M, Iwata S, Hasegawa N

J Infect Dis Ther 2 2014

Research paper (scientific journal), Joint Work,  ISSN  2332-0877

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Lewis Lung Carcinoma Progression Is Facilitated by TIG-3 Fibroblast Cells.

Yamauchi Y, Izumi Y, Asakura K, Kawai K, Wakui M, Ohmura M, Suematsu M, Nomori H.

Anticancer Res. 33 ( 9 ) 3791 - 3798 2013

Research paper (scientific journal), Joint Work, Accepted

Analysis of single nucleotide polymorphisms (SNPs)

Wakui M

Rinsho Byori 61 ( 11 ) 1008 - 1017 2013

Research paper (scientific journal), Single Work, Accepted

フィブリノゲン測定試薬を用いたアルガトロバンモニタリングの検討

Fujimori Yuta

第2回　血栓止血検査研究会－Tokyo  2012.07

Single Work

重症肝疾患患者で重要となる凝固・線溶マーカー.

WAKUI Masatoshi

Thrombosis Medicine 2 ( 3 ) 219 - 226 2012

Research paper (scientific journal), Single Work