Details of a Researcher - Amagai, Masayuki

Papers - Amagai, Masayuki

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Early symptoms preceding post-infectious irritable bowel syndrome following COVID-19: a retrospective observational study incorporating daily gastrointestinal symptoms

Yamamoto R., Yamamoto A., Masaoka T., Homma K., Matsuoka T., Takemura R., Wada M., Sasaki J., Kanai T., Amagai M., Saya H., Nishihara H.

BMC Gastroenterology (BMC Gastroenterology) 23 ( 1 ) 2023.12

Background: Intestinal microinflammation with immune dysfunction due to severe acute respiratory syndrome coronavirus 2 reportedly precipitates post-infectious irritable bowel syndrome. This study aimed to elucidate potential risk factors for subsequent development of irritable bowel syndrome, hypothesizing that it is associated with specific symptoms or patient backgrounds. Methods: This single-center retrospective observational study (2020–2021) included adults with confirmed coronavirus disease requiring hospital admission and was conducted using real-world data retrieved from a hospital information system. Patient characteristics and detailed gastrointestinal symptoms were obtained and compared between patients with and without coronavirus disease-induced irritable bowel syndrome. Multivariate logistic models were used to validate the risk of developing irritable bowel syndrome. Moreover, daily gastrointestinal symptoms during hospitalization were examined in patients with irritable bowel syndrome. Results: Among the 571 eligible patients, 12 (2.1%) were diagnosed with irritable bowel syndrome following coronavirus disease. While nausea and diarrhea during hospitalization, elevated white blood cell count on admission, and intensive care unit admission were associated with the development of irritable bowel syndrome, nausea and diarrhea were identified as risk factors for its development following coronavirus disease, as revealed by the adjusted analyses (odds ratio, 4.00 [1.01–15.84] and 5.64 [1.21–26.31], respectively). Half of the patients with irritable bowel syndrome had both diarrhea and constipation until discharge, and constipation was frequently followed by diarrhea. Conclusions: While irritable bowel syndrome was rarely diagnosed following coronavirus disease, nausea and diarrhea during hospitalization precede the early signs of irritable bowel syndrome following coronavirus disease.

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Dissecting skin microbiota and microenvironment for the development of therapeutic strategies

Ito Y., Amagai M.

Current Opinion in Microbiology (Current Opinion in Microbiology) 74 2023.08

ISSN 13695274

The skin is a pivotal barrier between the human body and the environment, and is a habitat for numerous microorganisms. While host–microbiota interactions in the skin are essential for homeostasis, disturbances in microbial composition and the abnormal growth of certain bacteria are associated with various diseases. Here, we identify strains and communities of skin commensals that contribute to or impair skin barrier function. Furthermore, we discuss the skin microenvironments suitable for specific microbiota that exert therapeutic effects and suggest focus areas for the prospective development of therapeutic strategies using bacterial agents. Finally, we highlight recent efforts to treat skin diseases associated with live bacteria.

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Diverse Role of OX40 on T Cells as a Therapeutic Target for Skin Diseases

Iriki H., Takahashi H., Amagai M.

Journal of Investigative Dermatology (Journal of Investigative Dermatology) 143 ( 4 ) 545 - 553 2023.04

ISSN 0022202X

OX40 is an important costimulatory molecule for T-cell expansion and survival. Because OX40 is expressed on most T-cell subsets, it is an attractive therapeutic target for a variety of T-cell‒mediated diseases. Clinical trials are already underway for some skin inflammatory diseases. In this review, we present various observations that improve our understanding of how OX40-targeted therapy can be applied for skin inflammatory diseases, such as atopic dermatitis and psoriasis, T helper (Th)2- and Th17-mediated diseases, respectively. The important OX40/OX40L-mediated interaction between T cells and other immune cells is also discussed in terms of skin autoimmune diseases, such as alopecia areata and pemphigus. Regulatory T cells (Tregs) highly express OX40, and the skin harbors a large Treg population; thus, understanding how OX40-targeted treatment acts on Tregs is vital for the development of therapeutic strategies for various skin diseases.

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Clinical severity scores as a guide for prediction of initial treatment responses in pemphigus and pemphigoid patients

Tanaka R., Kurihara Y., Egami S., Saito Y., Ouchi T., Funakoshi T., Takahashi H., Umegaki-Arao N., Kubo A., Tanikawa A., Amagai M., Yamagami J.

Journal of Dermatology (Journal of Dermatology) 50 ( 2 ) 203 - 211 2023.02

ISSN 03852407

Pemphigus and pemphigoid are autoimmune blistering diseases that affect mucosa and skin. Several clinical scoring systems, including the pemphigus disease area index (PDAI) and the bullous pemphigoid disease area index (BPDAI), have been validated for managing disease activity and severity. Current guidelines recommend that treatment response be evaluated with clinical scores and that additional second-line therapies be considered if initial treatment is insufficient for disease control. However, there have been few studies analyzing correlations between PDAI/BPDAI transitions and initial treatment effects. To investigate whether PDAI/BPDAI transitions during the treatment initiation phase correlate with initial treatment responses and whether such information can be used as a guide for necessary additional treatment, we retrospectively analyzed 67 pemphigus patients and 47 pemphigoid patients who received initial treatment at Keio University between 2012 and 2018. The clinical symptoms were evaluated weekly with PDAI/BPDAI. The patients were divided into two groups: in group A, disease was controlled only with oral corticosteroids and immunosuppressants (initial treatment), whereas in group B additional therapies were required due to insufficient responses. In pemphigus, the PDAI ratio of day 7/day 0 was significantly reduced in group A compared to group B (0.548 vs 0.761, P < 0.01) after initial treatment had started. In pemphigoid, the ratios of day 7/day 0 of BPDAI (erosion/blister) and BPDAI (urticaria/erythema) significantly decreased in group A compared to group B (0.565 vs 0.901 and 0.350 vs 0.760, respectively, P < 0.05). Receiver operating characteristic analyses on PDAI, BPDAI (erosion/blister) and BPDAI (urticaria/erythema) revealed that the cut-off values in the ratios of day 7/day 0 were 0.762, 0.675, and 0.568, respectively. Our results suggest that PDAI/BPDAI transitions during the initial phase of the treatments may be useful to predict the outcome of the treatment provided and the necessity of additional therapies to achieve disease control.

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Rituximab therapy for intractable pemphigus: A multicenter, open-label, single-arm, prospective study of 20 Japanese patients

Yamagami J., Kurihara Y., Funakoshi T., Saito Y., Tanaka R., Takahashi H., Ujiie H., Iwata H., Hirai Y., Iwatsuki K., Ishii N., Sakurai J., Abe T., Takemura R., Mashino N., Abe M., Amagai M.

Journal of Dermatology (Journal of Dermatology) 50 ( 2 ) 175 - 182 2023.02

ISSN 03852407

This was a multicenter clinical trial of rituximab, a chimeric monoclonal IgG antibody directed against CD20, for the treatment of refractory pemphigus vulgaris and pemphigus foliaceus. In total, 20 patients were treated with two doses of rituximab (1000 mg; 2 weeks apart) on days 0 and 14. The primary end point was the proportion of patients who achieved complete or partial remission on day 168 following the first rituximab dose. Of the 20 enrolled patients, 11 (55%) and four (20%) achieved complete and partial remission, respectively; therefore, remission was achieved in a total of 15 patients (75.0% [95% confidence interval, 50.9%–91.3%]). It was demonstrated that the remission rate was greater than the prespecified threshold (5%). In addition, a significant improvement in clinical score (Pemphigus Disease Area Index) and decrease in serum anti-desmoglein antibody level were observed over time. Four serious adverse events (heart failure, pneumonia, radial fracture, and osteonecrosis) were recorded in two patients, of which only pneumonia was considered causally related with rituximab. The level of peripheral blood CD19-positive B lymphocytes was decreased on day 28 after rituximab treatment and remained low throughout the study period until day 168. Our results confirm the efficacy and safety of rituximab therapy for refractory pemphigus in Japanese patients.

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Two sporadic cases of childhood-onset Hailey-Hailey disease with superimposed mosaicism

Asahina Y., Tahara U., Aoki S., Nakabayashi K., Tateishi C., Hayashi D., Amagai M., Tsuruta D., Kubo A.

European Journal of Human Genetics (European Journal of Human Genetics) 2023

ISSN 10184813

A prenatal second-hit genetic change that occurs on the wild-type allele in an embryo with a congenital pathogenic variant allele results in mosaicism of monoallelic and biallelic defect of the gene, which is called superimposed mosaicism. Superimposed mosaicism of Hailey-Hailey disease (HHD) has been demonstrated in one familial case. Here, we report two unrelated HHD cases with superimposed mosaicism: a congenital monoallelic pathogenic variant of ATP2C1, followed by a postzygotic copy-neutral loss of heterozygosity. Uniquely, neither patient had a family history of HHD at the time of presentation. In the first case, the congenital pathogenic variant had occurred de novo. In the second case, the father had the pathogenic variant but had not yet developed skin symptoms. Our cases showed that superimposed mosaicism in HHD can lack a family history and that genetic analysis is crucial to classify the type of mosaicism and evaluate the risk of familial occurrence.

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Desmoglein-Specific B-Cell−Targeted Single-Cell Analysis Revealing Unique Gene Regulation in Patients with Pemphigus

Egami S., Watanabe T., Fukushima-Nomura A., Nomura H., Takahashi H., Yamagami J., Ohara O., Amagai M.

Journal of Investigative Dermatology (Journal of Investigative Dermatology) 2023

ISSN 0022202X

Autoreactive B cells are assumed to play a critical role in pemphigus; however, the characteristics of these cells are not yet fully understood. In this study, 23 pemphigus vulgaris or pemphigus foliaceus samples were used to isolate circulating desmoglein (DSG)-specific B cells. Transcriptome analysis of the samples was performed at the single-cell level to detect genes involved in disease activity. DSG1- or DSG3-specific B cells from three patients’ differentially expressed genes related to T cell costimulation (CD137L) as well as B-cell differentiation (CD9, BATF, TIMP1) and inflammation (S100A8, S100A9, CCR3), compared with nonspecific B cells from the same patients. When the DSG1-specific B cells before and after treatment transcriptomes of the patient with pemphigus foliaceus were compared, there were changes in several B-cell activation pathways not detected in non−DSG1-specific B cells. This study clarifies the transcriptomic profile of autoreactive B cells in patients with pemphigus and documents the gene expression related to disease activity. Our approach can be applied to other autoimmune diseases and has the potential for future detection of disease-specific autoimmune cells.

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Controlling skin microbiome as a new bacteriotherapy for inflammatory skin diseases

Ito Y., Amagai M.

Inflammation and Regeneration (Inflammation and Regeneration) 42 ( 1 ) 2022.12

The skin serves as the interface between the human body and the environment and interacts with the microbial community. The skin microbiota consists of microorganisms, such as bacteria, fungi, mites, and viruses, and they fluctuate depending on the microenvironment defined by anatomical location and physiological function. The balance of interactions between the host and microbiota plays a pivotal role in the orchestration of skin homeostasis; however, the disturbance of the balance due to an alteration in the microbial communities, namely, dysbiosis, leads to various skin disorders. Recent developments in sequencing technology have provided new insights into the structure and function of skin microbial communities. Based on high-throughput sequencing analysis, a growing body of evidence indicates that a new treatment using live bacteria, termed bacteriotherapy, is a feasible therapeutic option for cutaneous diseases caused by dysbiosis. In particular, the administration of specific bacterial strains has been investigated as an exclusionary treatment strategy against pathogens associated with chronic skin disorders, whereas the safety, efficacy, and sustainability of this therapeutic approach using isolated live bacteria need to be further explored. In this review, we summarize our current understanding of the skin microbiota, as well as therapeutic strategies using characterized strains of live bacteria for skin inflammatory diseases. The ecosystem formed by interactions between the host and skin microbial consortium is still largely unexplored; however, advances in our understanding of the function of the skin microbiota at the strain level will lead to the development of new therapeutic methods.

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Pro108Ser mutation of SARS-CoV-2 3CL<sup>pro</sup> reduces the enzyme activity and ameliorates the clinical severity of COVID-19

Abe K., Kabe Y., Uchiyama S., Iwasaki Y.W., Ishizu H., Uwamino Y., Takenouchi T., Uno S., Ishii M., Maruno T., Noda M., Murata M., Hasegawa N., Saya H., Kitagawa Y., Fukunaga K., Amagai M., Siomi H., Suematsu M., Kosaki K.

Scientific Reports (Scientific Reports) 12 ( 1 ) 2022.12

Recently, an international randomized controlled clinical trial showed that patients with SARS-CoV-2 infection treated orally with the 3-chymotrypsin-like protease (3CLpro) inhibitor PF-07321332 within three days of symptom onset showed an 89% lower risk of COVID-19-related hospital admission/ death from any cause as compared with the patients who received placebo. Lending support to this critically important result of the aforementioned trial, we demonstrated in our study that patients infected with a SARS-Cov-2 sub-lineage (B.1.1.284) carrying the Pro108Ser mutation in 3CLpro tended to have a comparatively milder clinical course (i.e., a smaller proportion of patients required oxygen supplementation during the clinical course) than patients infected with the same sub-lineage of virus not carrying the mutation. Characterization of the mutant 3CLpro revealed that the Kcat/Km of the 3CLpro enzyme containing Ser108 was 58% lower than that of Pro108 3CLpro. Hydrogen/deuterium-exchange mass spectrometry (HDX-MS) revealed that the reduced activity was associated with structural perturbation surrounding the substrate-binding region of the enzyme, which is positioned behind and distant from the 108th amino acid residue. Our findings of the attenuated clinical course of COVID-19 in patients infected with SARS-CoV-2 strains with reduced 3CLpro enzymatic activity greatly endorses the promising result of the aforementioned clinical trial of the 3CLpro inhibitor.

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Microbiota-Independent Spontaneous Dermatitis Associated with Increased Sebaceous Lipid Production in Tmem79-Deficient Mice

Morimoto A., Fukuda K., Ito Y., Tahara U., Sasaki T., Shiohama A., Kawasaki H., Kawakami E., Naganuma T., Arita M., Sasaki H., Koseki H., Matsui T., Amagai M.

Journal of Investigative Dermatology (Journal of Investigative Dermatology) 142 ( 11 ) 2864 - 2872.e6 2022.11

ISSN 0022202X

TMEM79 is a predisposing gene for atopic dermatitis. Tmem79-deficient mice develop spontaneous dermatitis in a biphasic pattern. The first-phase dermatitis is unique because it occurs independent of microbiota status, whereas the second-phase dermatitis is microbiota dependent. In this study, we sought to identify the key factors mediating the development of first-phase dermatitis. Structural analysis showed that sebaceous gland hyperplasia started from first-phase dermatitis. Longitudinal RNA sequencing analysis revealed significant activation of fatty acid lipid metabolism pathways in first-phase dermatitis, whereas T helper 17‒based immune response genes were highly expressed in second-phase dermatitis. Quantitative RT-PCR analysis revealed that genes involved in fatty acid elongation and sebocyte differentiation were upregulated in first-phase dermatitis. The results of thin-layer chromatography supported these findings with an increased abundance of wax esters, cholesterol esters, and fatty alcohols in hair lipids. Further gas chromatography-tandem mass spectrometry analysis showed an increase in total fatty acid production, including that of elongated C20–24 saturated and C18–24 monounsaturated fatty acids. Collectively, these results suggest that aberrant production of sebaceous long-chain fatty acids is associated with microbiota-independent dermatitis. Further investigation of Tmem79-deficient mice may clarify the role of certain fatty acids in dermatitis.

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Neurofibromatosis type 2 with mild Pierre-Robin sequence showing a heterozygous chromosome 22q12 microdeletion encompassing NF2 and MN1

Saito S., Ono N., Sasaki T., Aoki S., Kosaki K., Kuze B., Nakabayashi K., Amagai M., Kubo A.

Journal of Human Genetics (Journal of Human Genetics) 67 ( 11 ) 675 - 678 2022.11

ISSN 14345161

Pierre-Robin sequence (PRS) is a rare, congenital defect presenting with micrognathia, glossoptosis, and airway obstruction with variable inclusion of a cleft palate. Overlapping PRS with neurofibromatosis type 2 (NF2) is a syndrome caused by a chromosome 22q12 microdeletion including NF2. We describe a patient with severe early-onset NF2 overlapping with PRS that showed micrognathia, glossoptosis, and a mild form of cleft palate. We detected a de novo chromosome 22q12 microdeletion including MN1 and NF2 in the patient. Previous cases of overlapping PRS and NF2 caused by the chromosome 22q12 microdeletions showed severe NF2 phenotypes with variable severity of cleft palate and microdeletions of varying sizes. Genotype-phenotype correlations and comparison of the size and breakpoint of microdeletions suggest that some modifier genes distal to MN1 and NF2 might be linked to the cleft palate severity.

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Severe graft-versus-host disease-like enterocolitis accompanied with cytomegalovirus-reactivation in drug-induced hypersensitivity syndrome/drug reaction with eosinophilia and systemic symptoms

Takamiyagi S., Iriki H., Asahina Y., Furuichi Y., Funakoshi T., Ichikawa M., Mikami Y., Okita H., Sakiyama T., Inazumi T., Amagai M., Takahashi H.

Journal of Dermatology (Journal of Dermatology) 49 ( 8 ) 796 - 799 2022.08

ISSN 03852407

Drug-induced hypersensitivity syndrome (DIHS)/drug reaction with eosinophilia and systemic symptoms (DRESS) is a severe drug adverse reaction with skin eruption and visceral organ involvement. The characteristic clinical features of DIHS/DRESS are reactivation of human herpesviruses (HHV) and the development of autoimmune diseases, but their pathogenesis and associations are not yet understood. Here, we report a 66-year-old man who presented with fever, generalized erythema, diffuse lymphadenopathy, and diarrhea after 3 weeks of treatment with zonisamide. Reactivation of HHV-6 and cytomegalovirus (CMV) was detected during the clinical course. The patient was diagnosed with DIHS/DRESS and treated with systemic prednisolone, i.v. immunoglobulin therapy, and ganciclovir. However, severe enterocolitis persisted for 6 months. A series of examinations revealed features of both CMV enterocolitis, as indicated by identification of a few CMV-positive cells on immunohistochemical analysis, and graft-versus-host disease (GVHD)-like enterocolitis indicated by orange-peel appearance on endoscopic examination and histopathological loss of goblet cells. Intractable enterocolitis continued and the patient finally died of pneumonia. An autoimmune predisposition in DIHS/DRESS patients in combination with CMV reactivation was considered to trigger the severe enterocolitis of this case that showed GVHD-like features of the gastrointestinal tract. GVHD-like organ damage is a pathological condition rarely observed in DIHS/DRESS but should be recognized as one of the most severe complications of the disease.

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Intravenous Ig Regulates Anti-Desmoglein 3 IgG Production in B220<sup>–</sup> Antibody-Producing Cells in Mice with Pemphigus Vulgaris

Kase Y., Takahashi H., Ito H., Kamata A., Amagai M., Yamagami J.

Journal of Investigative Dermatology (Journal of Investigative Dermatology) 142 ( 7 ) 1786 - 1792.e3 2022.07

ISSN 0022202X

Intravenous Ig (IVIG) is a treatment option for intractable cases of pemphigus vulgaris (PV), an autoimmune blistering disease caused by autoantibodies against desmoglein 3 (DSG3). To investigate the efficacy of IVIG on autoantibody secretion, we produced PV model mice by adoptive transfer of immunized Dsg3−/− splenocytes to Rag2−/− mice. We found that circulating anti-DSG3 IgG ELISA titer decreased in PV model mice after 5 days of treatment with IVIG compared with PBS-treated mice, whereas the F(ab’)2 fragment did not suppress the anti-DSG3 IgG titer. enzyme-linked immunospot assay revealed that IVIG treatment reduced the frequency of anti-DSG3 antibody–secreting cells in the spleen but not in lymph nodes and bone marrow. Moreover, this reduction was observed only in the splenic B220− fraction but not in the B220+ fraction. Furthermore, IVIG decreased the serum levels of anti-DSG3 IgG, even after a significant reduction of its titer, owing to antibody-mediated CD20+ B cell depletion. In addition, IVIG suppressed anti-DSG3 IgG production in B220−CD138+ plasma cells derived from PV model mice ex vivo. These results indicate that IVIG reduced autoantibody production in B220− cells containing plasma cells in PV model mice, and this function may indicate one of the mechanisms of action of IVIG on PV.

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Superimposition of checkerboard distribution of ephelides and neurofibromas in a patient with segmental neurofibromatosis

Iriki H., Umegaki-Arao N., Kakuta R., Fujita H., Aoki S., Amagai M., Sasaki T., Hamamoto Y., Nakayama R., Kubo A.

JAAD Case Reports (JAAD Case Reports) 25 89 - 92 2022.07

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【手足の皮膚病】関節リウマチの症状増悪に伴い出現したBywaters' lesions

田村佳奈, 角田梨沙, 金子祐子, 天谷雅行, 谷川瑛子

皮膚病診療 ((株)協和企画) 44 ( 7 ) 604 - 607 2022.07

ISSN 0387-7531

＜文献概要＞症例のポイント　・各種治療に抵抗性の30年来の関節リウマチの患者に,手指爪囲の紫斑や血疱が出現し,病理組織像で真皮小血管の閉塞像が確認されたことからBywaters' lesions(BL)と診断した.・治療経過中,関節リウマチの血清抗体価上昇および関節症状・関節外症状の出現など関節リウマチが増悪するたびにBLが出現する傾向がみられた.・関節リウマチ患者においてBLを認めた際には,病勢増悪や血管炎を疑わせる症状の有無に注意して,慎重に経過を追うことが大切である.

Highly Precise, Continuous, Long-Term Monitoring of Skin Electrical Resistance by Nanomesh Electrodes

Miyamoto A., Kawasaki H., Lee S., Yokota T., Amagai M., Someya T.

Advanced Healthcare Materials (Advanced Healthcare Materials) 11 ( 10 ) 2022.05

ISSN 21922640

The transepidermal water loss has been widely used as a method for directly evaluating the barrier function of the stratum corneum of the skin. However, transepidermal water loss could not be measured continuously for a long period of time, and there were no reports of continuous monitoring of skin barrier functions. Here, a method is reported to continuously monitor the skin electrical resistance by nanomesh electrodes for a long period of time while maintaining the natural skin condition that does not inhibit water evaporation. Simultaneous measurements of the skin electrical resistance by nanomesh electrodes and transepidermal water loss exhibits a linear fit with a high negative correlation. Furthermore, dynamics of skin physiological functions are successfully visualized by monitoring of the skin electrical resistance by nanomesh electrodes for 30 h in daily life.

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Increase in the Intracellular Bulk Water Content in the Early Phase of Cell Death of Keratinocytes, Corneoptosis, as Revealed by 65 GHz Near-Field CMOS Dielectric Sensor

Shiraga K., Ogawa Y., Kikuchi S., Amagai M., Matsui T.

Molecules (Molecules) 27 ( 9 ) 2022.05

While bulk water and hydration water coexist in cells to support the expression of biological macromolecules, how the dynamics of water molecules, which have long been only a minor role in molecular biology research, relate to changes in cellular states such as cell death has hardly been explored so far due to the lack of evaluation techniques. In this study, we developed a high-preci-sion measurement system that can discriminate bulk water content changes of ±0.02% (0.2 mg/cm3) with single-cell-level spatial resolution based on a near-field CMOS dielectric sensor operating at 65 GHz. We applied this system to evaluate the temporal changes in the bulk water content during the cell death process of keratinocytes, called corneoptosis, using isolated SG1 (first layer of stratum granulosum) cells in vitro. A significant irreversible increase in the bulk water content was observed approximately 1 h before membrane disruption during corneoptosis, which starts with cytoplasmic high Ca2+ signal. These findings suggest that the calcium flux may have a role in triggering the increase in the bulk water content in SG1 cells. Thus, our near-field CMOS dielectric sensor provides a valuable tool to dissect the involvement of water molecules in the various events that occur in the cell.

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【(新規)抗腫瘍薬〜光と影〜】ドセタキセル・ゲムシタビン併用療法と放射線治療が奏効した頭部血管肉腫

鎌田亜紀, 平井郁子, 中村善雄, 齋藤昌孝, 天谷雅行, 舩越建

皮膚病診療 ((株)協和企画) 44 ( 5 ) 406 - 409 2022.05

ISSN 0387-7531

【遺伝性疾患と遺伝カウンセリング】NCSTN遺伝子変異を同定した家族性化膿性汗腺炎/familial acne inversaの姉妹例

小野紀子, 田中諒, 中林一彦, 秦健一郎, 鈴木寿人, 小崎健次郎, 天谷雅行, 久保亮治

皮膚病診療 ((株)協和企画) 44 ( 3 ) 215 - 219 2022.03

ISSN 0387-7531

＜文献概要＞症例のポイント　・53歳女.高校生ごろからざ瘡が多発し,頸部から体幹,四肢にかけて大小の嚢胞性皮下腫瘤が散在.腋窩・鼠径部に瘻孔を形成,排膿を繰り返していた.・本人および妹に家族性化膿性汗腺炎/familial acne inversaの病原性変異として報告されているNCSTNのナンセンス変異を同定した.・家族性化膿性汗腺炎/familial acne inversaは常染色体優性遺伝のため,次世代に遺伝する確率は50%である.妹には未成年の子どもが3人いるが,未発症未成年の遺伝学的検査は本人が成人してから本人の意思で施行するのが原則である.

【遺伝性疾患と遺伝カウンセリング】母子間で皮疹の分布範囲が大きく異なったGoltz症候群

田原海, 小野紀子, 青木里美, 天谷雅行, 久保亮治

皮膚病診療 ((株)協和企画) 44 ( 3 ) 232 - 235 2022.03

ISSN 0387-7531

＜文献概要＞症例のポイント　・PORCNの片アレル欠失によって発症した,Goltz症候群の母子例を報告した.母は生下時から両側の広大な範囲に皮疹を認めた一方で,娘は片側のごく限られた範囲にのみ皮疹を認めた.・母子間でGoltz症候群の皮疹の分布範囲が大きく異なった原因として,ランダムにおこったX染色体不活化の結果,皮膚組織において正常なPORCNを発現せず病変部をつくる細胞の割合に差があったと考えられた.PORCNに遺伝学的変化を有する場合でも,皮疹の範囲は発生初期におこるX染色体不活化の結果をそのまま反映することが示唆され,次子が同疾患に罹患した場合は姉妹間でも皮疹の範囲が大きく異なる可能性があることを両親に伝えた.

IgM to IgG Class Switching Is a Necessary Step for Pemphigus Phenotype Induction in Desmoglein 3-Specific B Cell Receptor Knock-in Mouse

Nomura H., Wada N., Takahashi H., Kase Y., Yamagami J., Egami S., Iriki H., Mukai M., Kamata A., Ito H., Fujii H., Ishikura T., Koseki H., Watanabe T., Yamada T., Ohara O., Koyasu S., Amagai M.

Journal of Immunology (Journal of Immunology) 208 ( 3 ) 582 - 593 2022.02

ISSN 00221767

Pemphigus vulgaris is an autoimmune blistering disease caused by IgG targeting desmoglein 3 (Dsg3), an adhesion molecule of keratinocytes. Anti-Dsg3 IgG production is prevented in healthy individuals, but it is unclear how Dsg3-specific B cells are regulated. To clarify the immunological condition regulating Dsg3-specific B cells, a pathogenic anti-Dsg3 Ig (AK23) knock-in mouse was generated. AK23 knock-in B cells developed normally without undergoing deletion or acquiring an anergic phenotype in vivo. The knock-in B cells showed Ca2+ influx upon IgM cross-linking and differentiated into AK23-IgG+ B cells after LPS and IL-4 stimulation in vitro that induced a pemphigus phenotype after adoptive transfer into Rag2-/- mice. However, the knock-in mouse itself produced AK23-IgM but little IgG without blisters in vivo. Dsg3 immunization and skin inflammation caused AK23-IgG production and a pemphigus phenotype in vivo. Furthermore, Fcgr2b deficiency or haploinsufficiency spontaneously induced AK23-IgG production and a pemphigus phenotype with poor survival rates in AK23 knock-in mice. To assess Fcgr2b involvement in Ig class-switch efficiency, postswitch transcripts of B cells were quantified and significantly higher in Fcgr2b-/- and Fcgr2b1/mice than wild-type mice in a gene dose-dependent manner. Finally, RNA sequencing revealed reduced expression of FCGR2B and FcγRIIB-related genes in patient B cells. These results indicated that Dsg3-specific B cells do not spontaneously perform pathogenic class switching in vivo, and pemphigus phenotype induction was prevented under normal conditions. Attenuated FcγRIIB signaling is also one of the drivers for pathogenic class switching and is consistent with immunological features identified from clinical samples. This study unveiled a characteristic immune state silencing autoreactive B cells in mice.

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Analysis of clinical characteristics, prognosis and antibody pathogenicity of pemphigus patients positive for anti-desmoglein IgG autoantibodies in remission: a retrospective cohort study

Zhao W.L., Ishii K., Egami S., Xu Z., Funakoshi T., Takahashi H., Tanikawa A., Ishiko A., Amagai M., Yamagami J.

Journal of the European Academy of Dermatology and Venereology (Journal of the European Academy of Dermatology and Venereology) 36 ( 2 ) 271 - 278 2022.02

ISSN 09269959

Background: The detection of serum anti-desmoglein (Dsg) IgG autoantibodies has been reported to be useful for assessment of disease activity in pemphigus. However, previous studies have reported that anti-Dsg autoantibodies remain detectable in some patients without active pemphigus lesions. Objectives: To investigate the clinical characteristics and antibody pathogenicity of pemphigus patients positive for anti-Dsg IgG autoantibodies in remission. Methods: We retrospectively investigated pemphigus patients with a history of clinical remission who visited the Department of Dermatology of Keio University during 2019 and 2020. The antibody pathogenicity was assessed by bead aggregation assay. Results: When patients were recognized as having entered remission (PDAI = 0 and PSL ≦ 10 mg/day for 2 months), serum autoantibodies against Dsg were detected in 72 of 132 patients (54.5%, positive group; PG), but were not detected in 60 patients (45.5%, negative group; NG). Anti-Dsg antibody titres in remission declined from the active phase in 33 patients in the PG for whom data were available. There were no differences in the chance of reducing PSL to 5 mg/day (P = 0.885) and rate of relapse (P = 0.279) between PG and NG, but fewer patients in PG discontinued corticosteroids (P = 0.004). The ability of patients' sera to block aggregation of Dsg/desmocollin beads was significantly reduced in remission compared to the active phase. However, our results revealed that whole sera in remission still had pathogenic activity in seven of nine patients, and the approximately equal amounts of anti-Dsg antibodies in active phase and remission showed similar pathogenicity. Conclusions: This study will provide guidance in cases where autoantibodies are found to be positive in pemphigus patients during remission or steroid reduction.

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Diagnostic utility of the basophil activation test in natto-induced hypersensitivity

Fukuda R., Ouchi T., Asahina Y., Shiiya C., Takeuchi S., Yasuda-Sekiguchi F., Iriki H., Kouno M., Takahashi S., Tanikawa A., Amagai M., Takahashi H.

Allergology International (Allergology International) 71 ( 1 ) 125 - 130 2022.01

ISSN 13238930

Background: Natto (fermented soybeans)-induced hypersensitivity is characterized by delayed symptom onset that hampers diagnosis. We aimed to clarify the clinical utility of the basophil activation test (BAT) in the diagnosis of natto-induced hypersensitivity. Methods: Five patients with a history of anaphylaxis and chronic urticaria suspected of natto-induced hypersensitivity and seven with chronic spontaneous urticaria clinically unrelated to natto were enrolled in the patient and control groups, respectively. The BAT was performed with two incubation times, 15 min and 1 h, in combination with various concentrations of natto-mucilage extract. Results: In controls, CD203c levels in basophils remained low in the 15-min incubation but were significantly increased in the 1-h incubation. In the patient group, in the 15-min condition, basophil CD203c was significantly upregulated by natto mucilage but not by soybean vs controls (P = 0.001). Low concentrations of natto mucilage were sufficient to upregulate basophil CD203c in the anaphylaxis cases, but high concentrations were required to induce the same effect in the urticaria cases. Finally, the dose-dependent pattern of the BAT results differed significantly between the anaphylaxis and urticaria cases (P = 0.006). Thus, a strong background reaction was observed in the BAT with 1 h incubation; 15 min of incubation was sufficient to identify patients with natto-induced hypersensitivity and may distinguish the clinical phenotype of natto-induced hypersensitivity, i.e., anaphylaxis or urticaria. Conclusions: The BAT with a 15-min incubation period is useful in diagnosing natto-induced hypersensitivity.

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Imatinib mesylate in combination with pembrolizumab in patients with advanced KIT -mutant melanoma following progression on standard therapy: A phase I/II trial and study protocol

Hirai I., Tanese K., Fukuda K., Fusumae T., Nakamura Y., Sato Y., Amagai M., Funakoshi T.

Medicine (United States) (Medicine (United States)) 100 ( 49 ) 2021.12

ISSN 00257974

Introduction:Genetic alterations of KIT gene are known to be one of the major causes of melanoma. Those are more common in the mucous and acral subtypes and KIT is regarded as major oncogene in Asian melanomas, where the prevalence of these subtypes is high. Up to date, several clinical trials have been conducted to target KIT gene alterations in melanoma with unsatisfied efficacies. Imatinib mesylate, a small-molecule inhibitor of the KIT tyrosine kinase, provides a rapid but not durable clinical response in KIT-mutant melanoma. Meanwhile, recent basic and clinical evidence have revealed another aspect of KIT-targeted therapy, namely the enhancement of antitumor activity of immune checkpoint inhibitors. Herein, we designed clinical trial of co-administrating imatinib mesylate and pembrolizumab (anti-PD-1 antibody) to evaluate its safety and efficacy.Methods and analysis:This is an open-label, single-arm, phase I/II clinical trial involving Japanese patients with metastatic KIT-mutant melanoma that are refractory to standard therapy including anti-PD-1 therapy. Phase I study is a dose-escalation study comprising two dose levels of imatinib mesylate (200 and 400 mg/day, respectively) with fixed dose of pembrolizumab (200 mg every 3 weeks) to evaluate safety and tolerability and determine recommended phase II dose. The primary endpoint of the phase II study is the objective response rate after 4 cycles (3 weeks/cycle) of pembrolizumab and imatinib mesylate at the dose determined in phase I, based on RECIST version 1.1. A Simon's minimax two-stage design is employed to test the null hypothesis of a 5% response rate vs 30% alternative, which will be rejected when a lower confidence limit of two-sided 90% confidence interval of true response rate is over than threshold response rate. The secondary endpoints include progression free survival, overall survival, best overall response and incidence of adverse events. Totally, a target size of 22 patients will be expected.Discussion:If this study shows efficacy and acceptable safety profile, it will contribute to the development of novel treatment option for patients with KIT-mutant melanoma that are refractory to standard therapy.Trial registration:NCT04546074. Date of Registration: September 11, 2020 (https://clinicaltrials.gov/ct2/show/NCT04546074). Date of First Participant Enrollment: December 23, 2020.

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Peripheral tolerance by treg via constraining OX40 signal in autoreactive T cells against desmoglein 3, a target antigen in pemphigus

Iriki H., Takahashi H., Wada N., Nomura H., Mukai M., Kamata A., Ito H., Yamagami J., Matsui T., Kurebayashi Y., Mise-Omata S., Nishimasu H., Nureki O., Yoshimura A., Hori S., Amagai M.

Proceedings of the National Academy of Sciences of the United States of America (Proceedings of the National Academy of Sciences of the United States of America) 118 ( 49 ) 2021.12

ISSN 00278424

Antigen-specific peripheral tolerance is crucial to prevent the development of organ-specific autoimmunity. However, its function decoupled from thymic tolerance remains unclear. We used desmoglein 3 (Dsg3), a pemphigus antigen expressed in keratinocytes, to analyze peripheral tolerance under physiological antigen-expression conditions. Dsg3-deficient thymi were transplanted into athymic mice to create a unique condition in which Dsg3 was expressed only in peripheral tissue but not in the thymus. When bone marrow transfer was conducted from high-avidity Dsg3-specific T cell receptor–transgenic mice to thymus-transplanted mice, Dsg3-specific CD4+ T cells developed in the transplanted thymus but subsequently disappeared in the periphery. Additionally, when Dsg3-specific T cells developed in Dsg32/2 mice were adoptively transferred into Dsg3-sufficient recipients, the T cells disappeared in an antigen-specific manner without inducing autoimmune dermatitis. However, Dsg3-specific T cells overcame this disappearance and thus induced autoimmune dermatitis in Treg-ablated recipients but not in Foxp3-mutant recipients with dysfunctional Tregs. The molecules involved in disappearance were sought by screening the transcriptomes of wild-type and Foxp3-mutant Tregs. OX40 of Tregs was suggested to be responsible. Consistently, when OX40 expression of Tregs was constrained, Dsg3-specific T cells did not disappear. Furthermore, Tregs obtained OX40L from dendritic cells in an OX40-dependent manner in vitro and then suppressed OX40L expression in dendritic cells and Birc5 expression in Dsg3-specific T cells in vivo. Lastly, CRISPR/Cas9-mediated knockout of OX40 signaling in Dsg3-specific T cells restored their disappearance in Treg-ablated recipients. Thus, Treg-mediated peripheral deletion of autoreactive T cells operates as an OX40-dependent regulatory mechanism to avoid undesired autoimmunity besides thymic tolerance.

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The pathogeneses of pemphigus and pemphigoid diseases

Ujiie H., Yamagami J., Takahashi H., Izumi K., Iwata H., Wang G., Sawamura D., Amagai M., Zillikens D.

Journal of Dermatological Science (Journal of Dermatological Science) 104 ( 3 ) 154 - 163 2021.12

ISSN 09231811

Autoimmune bullous diseases (AIBDs) are skin disorders which are mainly induced by autoantibodies against desmosomal or hemidesmosomal structural proteins. Previous studies using patients’ samples and animal disease models identified target antigens and elucidated the mechanisms of blister formation. Pemphigus has been the subject of more active clinical and basic research than any other AIBD. These efforts have revealed the pathogenesis of pemphigus, which in turn has led to optimal diagnostic methods and novel therapies, such as rituximab. In bullous pemphigoid (BP), studies with passive-transfer mouse models using rabbit anti-mouse BP180 antibodies and studies with passive-transfer or active mouse models using autoantigen-humanized mice elucidated the immune reactions to BP180 in vivo. Recently, dipeptidyl peptidase-4 inhibitors have attracted attention as a trigger for BP. For epidermolysis bullosa acquisita (EBA), investigations using mouse models are actively under way and several molecules have been identified as targets for novel therapies. In this review, we give an overview and discussion of the recent progress in our understanding of the pathogenesis of pemphigus, BP, and EBA. Further studies on the breakdown of self-tolerance and on the identification of key molecules that are relevant to blister formation may expand our understanding of the etiology of AIBDs and lead to the development of novel therapeutic strategies.

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Comprehensive and long-term surveys of COVID-19 sequelae in Japan, an ambidirectional multicentre cohort study: Study protocol

Nakagawara K., Namkoong H., Terai H., Masaki K., Tanosaki T., Shimamoto K., Lee H., Tanaka H., Okamori S., Kabata H., Chubachi S., Ikemura S., Kamata H., Yasuda H., Kawada I., Ishii M., Ishibashi Y., Harada S., Fujita T., Ito D., Bun S., Tabuchi H., Kanzaki S., Shimizu E., Fukuda K., Yamagami J., Kobayashi K., Hirano T., Inoue T., Kagyo J., Shiomi T., Ohgino K., Sayama K., Otsuka K., Miyao N., Odani T., Oyamada Y., Masuzawa K., Nakayama S., Suzuki Y., Baba R., Nakachi I., Kuwahara N., Ishiguro T., Mashimo S., Minematsu N., Ueda S., Manabe T., Funatsu Y., Koh H., Yoshiyama T., Saito F., Ishioka K., Takahashi S., Nakamura M., Goto A., Harada N., Kusaka Y., Nakano Y., Nishio K., Tateno H., Edahiro R., Takeda Y., Kumanogoh A., Kodama N., Okamoto M., Umeda A., Hagimura K., Sato T., Miyazaki N., Takemura R., Sato Y., Takebayashi T., Nakahara J., Mimura M., Ogawa K., Shimmura S., Negishi K., Tsubota K., Amagai M., Goto R., Ibuka Y., Hasegawa N., Kitagawa Y., Kanai T., Fukunaga K.

BMJ Open Respiratory Research (BMJ Open Respiratory Research) 8 ( 1 ) 2021.11

Introduction The rapid spread of COVID-19 posed a global burden. Substantial number of people died of the disease in the acute phase of infection. In addition, a significant proportion of patients have been reported to suffer from post-acute phase symptoms, sequelae of COVID-19, which may negatively influence the quality of daily living and/or socioeconomic circumstances of the patients. However, no previous study has comprehensively and objectively assessed the quality of life of patients by using existing international scales. Further, evidence of socioeconomic consequences among patients with COVID-19 is scarce. To address the multidimensional issues from sequelae of COVID-19, evidence from comprehensive surveys beyond clinical perspectives is critical that investigates health, and social determinants of disease progression as well as socioeconomic consequences at a large scale. Methods and analysis In this study, we plan to conduct a nationwide and comprehensive survey for the sequelae of COVID-19 in a total of 1000 patients diagnosed at 27 hospitals throughout Japan. This study will evaluate not only the health-related status of patients from clinical perspectives but also the Health-related Quality of Life (HRQoL) scores, socioeconomic status and consequences to discuss the sequelae of the disease and the related risk factors. The primary endpoint is the frequency of long-term complications of COVID-19 infection. The secondary endpoints are risk factors for progression to sequelae of COVID-19 infection. The study will provide robust and important evidence as a resource to tackle the issues from the sequelae of COVID-19 from the multi-dimensional perspectives. Ethics and dissemination This trial was approved by the Keio University School of Medicine Ethics Committee (20200243, UMIN000042299). The results of this study will be reported at a society meeting or published in a peer-reviewed journal.

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Filaggrin-gene mutation has minimal effect on the disease severity in the lesions of atopic dermatitis

Ota M., Sasaki T., Ebihara T., Yokosawa E., Murakami Y., Matsunaka H., Chinuki Y., Amagai M., Morita E.

Journal of Dermatology (Journal of Dermatology) 48 ( 11 ) 1688 - 1699 2021.11

ISSN 03852407

Loss-of-function mutations of filaggrin (FLG) gene (FLG) are the strongest known genetic risk factor for atopic dermatitis (AD). It is still debatable how FLG gene mutations and the resulting abnormal amount of FLG protein contribute to skin barrier function and symptoms of AD. In this study, we examined the effects of loss-of-function mutations of FLG gene on the severity of skin lesions and skin barrier function in 55 patients with AD by evaluating eight patients with AD with FLG gene mutations and 47 patients with AD without mutations. The results showed that the FLG gene mutation did not affect the duration of AD, severity of AD, degree of local inflammatory symptoms, skin water content and trans-epidermal water loss of the lesions. Next, in these eight mutation carriers and the 47 non-carriers, stratum corneum was collected from the three site of skin lesions using tape-stripping method, and the amounts of FLG protein and total amino acid contained in the stratum corneum was measured to investigate the effect of the FLG gene mutation on the amount of FLG gene product in the local lesion. FLG abnormalities had little effect on FLG protein and total amino acid content in the stratum corneum in the lesional skin. The amount of the FLG products, especially amino acids derived from FLG, in the stratum corneum of AD lesional skin is influenced by development of dermatitis. The results obtained from this study supports that the activation of Th2-dominant inflammatory cells, together with FLG abnormality, plays a role in suppressing the production of FLG in skin lesions.

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Frequent FGFR3 and Ras Gene Mutations in Skin Tags or Acrochordons

Aoki S., Hirata Y., Kawai T., Nakabayashi K., Hata K., Suzuki H., Kosaki K., Amagai M., Kubo A.

Journal of Investigative Dermatology (Journal of Investigative Dermatology) 141 ( 11 ) 2756 - 2760.e8 2021.11

ISSN 0022202X

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Efficacy of hydroxychloroquine for treating annular erythema associated with Sjögren’s syndrome

Arakawa H., Tanese K., Tanaka R., Murakami K., Sujino K., Miyamoto J., Amagai M., Tanikawa A.

Journal of Dermatology (Journal of Dermatology) 48 ( 10 ) 1526 - 1532 2021.10

ISSN 03852407

Annular erythema is one of the cutaneous manifestations of Sjögren’s syndrome (SS). Topical corticosteroids and tacrolimus, and oral corticosteroids, have been used as treatments for this condition. However, the safety and efficacy of these treatments remains unsatisfactory, and further development of therapies are desired. In this study, we performed a retrospective analysis of 16 annular erythema associated with SS (AESS) patients treated with hydroxychloroquine (HCQ). Disease activity was assessed using a modified version of the Cutaneous Lupus Erythematosus Disease Area and Severity Index (CLASI), which we termed the modified CLASI (m-CLASI). HCQ treatment improved AESS lesions in all 16 patients. The mean m-CLASI score was reduced by 85.6% at the 12-week follow-up relative to baseline (p < 0.01). Notably, 60% (6/10 cases) of patients with AESS lesions limited to the facial area achieved complete remission within 4 weeks. In the analysis of six patients who had taken oral prednisolone before starting HCQ, all were able to reduce the dose within 52 weeks without relapse. Particularly, 75% (3/4 cases) of patients with prednisolone dose of more than 5 mg/day could reduce their dose to less than 5 mg/day in combination with HCQ. For the safety concerns, two patients experienced grade 1 diarrhea during the 52-week observation period. However, neither serious adverse events nor adverse events requiring discontinuation of treatment occurred. The results of the present study suggest that HCQ may not only be highly effective as a single agent, but may also be useful as a steroid-sparing agent in refractory case requiring long-term steroid administration, making it a good treatment option for AESS.

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A multicenter, open-label, uncontrolled, single-arm phase 2 study of tirabrutinib, an oral Bruton's tyrosine kinase inhibitor, in pemphigus

Yamagami J., Ujiie H., Aoyama Y., Ishii N., Tateishi C., Ishiko A., Ichijima T., Hagihara S., Hashimoto K., Amagai M.

Journal of Dermatological Science (Journal of Dermatological Science) 103 ( 3 ) 135 - 142 2021.09

ISSN 09231811

Background: The treatment of pemphigus is based on systemic corticosteroid use and adjuvant therapies, but some patients are resistant to conventional therapy. Tirabrutinib is a highly selective oral Bruton's tyrosine kinase inhibitor that may be clinically effective in treating pemphigus by suppressing B-cell signaling. Objective: We investigated the efficacy and safety of tirabrutinib in patients with refractory pemphigus. Methods: This was a multicenter, open-label, single-arm phase 2 study of Japanese patients with refractory pemphigus receiving appropriate treatment with an oral corticosteroid and adjuvant therapies. Patients received postprandial oral tirabrutinib 80 mg once daily for 52 weeks. After 16 weeks of tirabrutinib treatment, the corticosteroid dose was tapered to ≤10 mg/day of prednisolone equivalent. Results: In total, 16 patients were evaluated (mean age, 52.5 years; 50 % male). The complete remission rate after 24 weeks of treatment (primary endpoint) was 18.8 % (3/16; 95 % confidence interval, 6.6 %–43.0 %). By Week 52, eight patients (50.0 %) achieved complete remission and 10 patients (62.5 %) achieved remission. Over 52 weeks of treatment, the mean prednisolone dose decreased from 17.03 to 7.65 mg/day. Incidences of adverse events (AEs) and adverse drug reactions were 87.5 % and 43.8 %, respectively. A relationship with tirabrutinib was ruled out for all serious AEs and Grade ≥3 AEs. Conclusion: Treatment with tirabrutinib enabled remission and reduced oral corticosteroid exposure over time and did not result in any major safety concerns in patients with refractory pemphigus. Thus, oral tirabrutinib may be a new treatment option for patients with refractory pemphigus.

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Neonatal Linear IgA Bullous Dermatosis Mediated by Breast Milk-Borne Maternal IgA

Egami S., Suzuki C., Kurihara Y., Yamagami J., Kubo A., Funakoshi T., Nishie W., Matsumura K., Matsushima T., Kawaida M., Sakamoto M., Amagai M.

JAMA Dermatology (JAMA Dermatology) 157 ( 9 ) 1107 - 1111 2021.09

ISSN 21686068

Importance: Neonatal linear immunoglobulin A (IgA) bullous dermatosis (LABD) is a rare disease that can be fatal when associated with respiratory failure. All previously reported cases of neonatal LABD have been in newborns with healthy asymptomatic mothers, and the pathogenic IgA was of unknown origin. Objective: To clarify the origin of IgA associated with LABD in neonates born of healthy asymptomatic mothers. Design, Setting, and Participants: This case study analyzed the laboratory findings of a single breast-fed newborn male with neonatal LABD admitted to the Keio University Hospital in Tokyo and his healthy asymptomatic mother. The healthy newborn developed life-threatening blisters and erosions of the skin and mucous membranes on day 4 after birth. Blood serum, skin, and maternal breast milk were examined for IgA autoantibodies. Main Outcomes and Measures: Histopathologic and immunofluorescence analyses of specimens (serum, skin, and breast milk) from the patient and his mother. Results: Histopathologic evaluation of the newborn's skin revealed subepidermal blisters with neutrophil infiltrates, and immunofluorescence testing showed linear IgA deposition along the basement membrane zone (BMZ), which lead to the diagnosis of neonatal LABD. Indirect immunofluorescence using normal human skin after treatment with 1-mol/L sodium chloride showed the patient to have circulating IgA binding to the dermal side of BMZ. Immunohistochemical staining proved the deposition of secretory IgA in the neonatal skin by demonstrating the presence of J chain - not been seen in other LABD cases - indicating that the autoantibodies producing the blisters were derived from the maternal breast milk. Although no circulating IgA against the skin was detected in mother's sera, the breast milk contained IgA that reacted with the dermal side of the BMZ. No new blister formation was observed after cessation of breastfeeding. Conclusions and Relevance: The results of this case study suggest a passive transfer of pathogenic IgA to a newborn from an asymptomatic mother via breast milk. In prior reports, no serum from asymptomatic mothers of newborns with LABD had IgA autoantibodies binding to skin components; however, in this case, we found that the maternal breast milk contained IgA autoantibodies associated with neonatal LABD. In neonatal LABD, maternal breast milk should be examined for IgA autoantibodies and breast milk feeding should be discontinued as soon as neonatal LABD is suspected..

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Adoptive cell therapy using tumor-infiltrating lymphocytes for melanoma refractory to immune-checkpoint inhibitors

Hirai I., Funakoshi T., Kamijuku H., Fukuda K., Mori M., Sakurai M., Koda Y., Kato J., Mori T., Watanabe N., Noji S., Yaguchi T., Iwata T., Ohta S., Fujita T., Tanosaki R., Handa M., Okamoto S., Amagai M., Kawakami Y.

Cancer Science (Cancer Science) 112 ( 8 ) 3163 - 3172 2021.08

ISSN 13479032

To evaluate the feasibility of adoptive cell therapy (ACT) using ex vivo-expanded tumor-infiltrating lymphocytes (TILs) in Japanese patients with melanoma who failed immune-checkpoint inhibitor therapy, an open-label, single-arm, pilot study was conducted. We investigated the immunological and genetic factors of the pretreatment tumor and expanded TILs that may be associated with the clinical response. The treatment protocol comprised preparation of TIL culture, lympho-depleting non-myeloablative preconditioning with cyclophosphamide and fludarabine, TIL infusion, and intravenous administration of low-dose IL-2. Three patients of clinical subtypes mucosal, superficial spreading, and acral melanoma underwent TIL-ACT. Most severe adverse events, including fever and leukopenia, were manageable with the supportive regimen specified in the protocol, suggesting that the TIL-ACT regimen is suitable for Japanese patients with melanoma. One patient showed a short-term partial response, one relatively long-stable disease, and one experienced disease progression. Whole-exome and transcriptional sequencing of isolated tumor cells and immunohistochemical analyses before TIL-ACT revealed various immunostimulatory factors, including a high tumor mutation burden and immune cell-recruiting chemokines, as well as various immunosuppressive factors including TGF-β, VEGF, Wnt/β-catenin, and MAPK signaling and epithelial-to-mesenchymal transition, which might influence the efficacy of TIL-ACT. Our results imply mechanisms for the antitumor effect of and resistance to TIL-ACT. Further studies of immune-resistant mechanisms of TIL-ACT are warranted. This study is registered with the UMIN Clinical Trial Registry (UMIN 000011431).

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Autosomal dominant familial acanthosis nigricans caused by a C-terminal nonsense mutation of FGFR3

Tahara U., Yasuda M., Yamada Y., Aoki S., Sato S., Amagai M., Kubo A.

Journal of Human Genetics (Journal of Human Genetics) 66 ( 8 ) 831 - 834 2021.08

ISSN 14345161

FGFR3 encodes a transmembrane receptor tyrosine kinase that has six autophosphorylation sites of tyrosine. Among them, Y770 is a negative regulatory site for the downstream signaling of FGFR3. Constitutive active mutations in FGFR3 are involved in human developmental disorders including familial acanthosis nigricans, an autosomal dominant disorder characterized by general hyperpigmentation with mild acanthosis of the epidermis. Here, we report two unrelated cases of familial acanthosis nigricans with a heterozygous c.2302G>T (p.E768*) mutation in FGFR3 (NM_000142.5). FGFR3 mRNA purified from the skin lesion neither showed aberrant splicing nor nonsense-mediated mRNA decay, indicating that the FGFR3 mutant simply lacked the C-terminal 768–806 amino acids including Y770. While all of the known pathogenic mutations were missense mutations in FGFR3 showing autosomal dominant trait, the c.2302G>T mutation of FGFR3 is a unique autosomal dominant nonsense mutation that causes familial acanthosis nigricans probably via loss of negative regulatory autophosphorylation site of FGFR3.

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爪甲紅色線条を呈した爪乳頭腫の1例

椎谷千尋, 種本紗枝, 宮本樹里亜, 天谷雅行, 齋藤昌孝

皮膚科の臨床 (金原出版(株)) 63 ( 9 ) 1417 - 1419 2021.08

ISSN 0018-1404

＜文献概要＞45歳,男性。左拇指爪甲の爪半月から爪甲遠位端まで伸びる幅2mmの紅色線条がみられた。爪甲遠位端には爪甲下に角質の付着を認め,同部位に非常に強い圧痛の訴えがあった。ダーモスコピーでは爪甲の下面が軽度陥凹しており,陥凹部では角質塊がみられた。紅色線条をすべて切除したところ,病理では紅色線条部の爪甲の低形成に加えて,爪床表皮の乳頭状増殖と真皮浅層の毛細血管拡張の所見を認めた。臨床および病理所見より,爪乳頭腫と診断した。術後速やかに疼痛は消失し,明らかな爪甲の変形も残さなかった。爪甲紅色線条を呈する疾患はいくつか知られており,そのひとつが爪乳頭腫である。爪乳頭腫はその病因や病態が未だ不明なこともあり,貴重な症例と考え報告した。

Risk of Stevens-Johnson syndrome and toxic epidermal necrolysis associated with anticonvulsants in a Japanese population: Matched case–control and cohort studies

Fukasawa T., Takahashi H., Takahashi K., Tanemura N., Amagai M., Urushihara H.

Allergology International (Allergology International) 70 ( 3 ) 335 - 342 2021.07

ISSN 13238930

Background: Evidence for the risk and incidence of anticonvulsant-induced Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) in Japan is scarce. Methods: We conducted a matched case–control study using a large-scale Japanese claims database. SJS/TEN cases were identified using a claims-based algorithm developed in a previous study (sensitivity 76.9%, specificity 99.0%). Conditional logistic regression with Firth's bias correction to address an issue of rare events was used to estimate odds ratios (ORs) for SJS/TEN for each anticonvulsant use (90 days before the index date) versus non-use. 90-day cumulative incidence of SJS/TEN per 100,000 new users was calculated for 33 anticonvulsants. Causality between anticonvulsant use and SJS/TEN in each exposed case was assessed using the algorithm of drug causality for epidermal necrolysis (ALDEN) score. Results: From 5,114,492 subjects, we selected 71 SJS/TEN cases and 284 controls. We observed significantly increased ORs for SJS/TEN among new users of carbamazepine (OR 68.00) and lamotrigine (OR 36.00) with ALDEN scores of “probable” or higher. Cumulative incidence of SJS/TEN was 93.83 for carbamazepine and 84.33 for lamotrigine. One case newly exposed to phenytoin which developed SJS/TEN was rated “unlikely” in ALDEN causality, resulting in cumulative incidence of 66.27. Cumulative incidence of SJS/TEN was 25.23 for levetiracetam, 7.52 for clonazepam, and 1.23 for diazepam, but their ALDEN scores were “very unlikely”. Conclusions: This study is the first to document the differential risk of SJS/TEN for anticonvulsants in a real-world setting in Japan. Exposure to carbamazepine and lamotrigine was associated with an increased risk of SJS/TEN.

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Atopic dermatitis: Role of the skin barrier, environment, microbiome, and therapeutic agents

Luger T., Amagai M., Dreno B., Dagnelie M.A., Liao W., Kabashima K., Schikowski T., Proksch E., Elias P.M., Simon M., Simpson E., Grinich E., Schmuth M.

Journal of Dermatological Science (Journal of Dermatological Science) 102 ( 3 ) 142 - 157 2021.06

ISSN 09231811

Atopic dermatitis (AD) is a chronic, inflammatory skin disorder characterized by eczematous and pruritic skin lesions. In recent decades, the prevalence of AD has increased worldwide, most notably in developing countries. The enormous progress in our understanding of the complex composition and functions of the epidermal barrier allows for a deeper appreciation of the active role that the skin barrier plays in the initiation and maintenance of skin inflammation. The epidermis forms a physical, chemical, immunological, neuro-sensory, and microbial barrier between the internal and external environment. Not only lesional, but also non-lesional areas of AD skin display many morphological, biochemical and functional differences compared with healthy skin. Supporting this notion, genetic defects affecting structural proteins of the skin barrier, including filaggrin, contribute to an increased risk of AD. There is evidence to suggest that natural environmental allergens and man-made pollutants are associated with an increased likelihood of developing AD. A compromised epidermal barrier predisposes the skin to increased permeability of these compounds. Numerous topical and systemic therapies for AD are currently available or in development; while anti-inflammatory therapy is central to the treatment of AD, some existing and novel therapies also appear to exert beneficial effects on skin barrier function. Further research on the skin barrier, particularly addressing epidermal differentiation and inflammation, lipid metabolism, and the role of bacterial communities for skin barrier function, will likely expand our understanding of the complex etiology of AD and lead to identification of novel targets and the development of new therapies.

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A unique mode of keratinocyte death requires intracellular acidification

Matsui T., Kadono-Maekubo N., Suzuki Y., Furuichi Y., Shiraga K., Sasaki H., Ishida A., Takahashi S., Okada T., Toyooka K., Sharif J., Abe T., Kiyonari H., Tominaga M., Miyawaki A., Amagai M.

Proceedings of the National Academy of Sciences of the United States of America (Proceedings of the National Academy of Sciences of the United States of America) 118 ( 17 ) 2021.04

ISSN 00278424

The stratum corneum (SC), the outermost epidermal layer, consists of nonviable anuclear keratinocytes, called corneocytes, which function as a protective barrier. The exact modes of cell death executed by keratinocytes of the upper stratum granulosum (SG1 cells) remain largely unknown. Here, using intravital imaging combined with intracellular Ca2+- and pH-responsive fluorescent probes, we aimed to dissect the SG1 death process in vivo. We found that SG1 cell death was preceded by prolonged (∼60 min) Ca2+ elevation and rapid induction of intracellular acidification. Once such intracellular ionic changes were initiated, they became sustained, irreversibly committing the SG1 cells to corneocyte conversion. Time-lapse imaging of isolated murine SG1 cells revealed that intracellular acidification was essential for the degradation of keratohyalin granules and nuclear DNA, phenomena specific to SC corneocyte formation. Furthermore, intravital imaging showed that the number of SG1 cells exhibiting Ca2+ elevation and the timing of intracellular acidification were both tightly regulated by the transient receptor potential cation channel V3. The functional activity of this protein was confirmed in isolated SG1 cells using whole-cell patch-clamp analysis. These findings provide a theoretical framework for improved understanding of the unique molecular mechanisms underlying keratinocyte-specific death mode, namely corneoptosis.

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Staphylococcus cohnii is a potentially biotherapeutic skin commensal alleviating skin inflammation

Ito Y., Sasaki T., Li Y., Tanoue T., Sugiura Y., Skelly A.N., Suda W., Kawashima Y., Okahashi N., Watanabe E., Horikawa H., Shiohama A., Kurokawa R., Kawakami E., Iseki H., Kawasaki H., Iwakura Y., Shiota A., Yu L., Hisatsune J., Koseki H., Sugai M., Arita M., Ohara O., Matsui T., Suematsu M., Hattori M., Atarashi K., Amagai M., Honda K.

Cell Reports (Cell Reports) 35 ( 4 ) 2021.04

Host-microbe interactions orchestrate skin homeostasis, the dysregulation of which has been implicated in chronic inflammatory conditions such as atopic dermatitis and psoriasis. Here, we show that Staphylococcus cohnii is a skin commensal capable of beneficially inhibiting skin inflammation. We find that Tmem79−/− mice spontaneously develop interleukin-17 (IL-17)-producing T-cell-driven skin inflammation. Comparative skin microbiome analysis reveals that the disease activity index is negatively associated with S. cohnii. Inoculation with S. cohnii strains isolated from either mouse or human skin microbiota significantly prevents and ameliorates dermatitis in Tmem79−/− mice without affecting pathobiont burden. S. cohnii colonization is accompanied by activation of host glucocorticoid-related pathways and induction of anti-inflammatory genes in the skin and is therefore effective at suppressing inflammation in diverse pathobiont-independent dermatitis models, including chemically induced, type 17, and type 2 immune-driven models. As such, S. cohnii strains have great potential as effective live biotherapeutics for skin inflammation.

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造影CTで検出困難であった活動性出血が外科的介入時に判明したdeep dissecting hematomaの1例

小川夕貴, 伏間江貴之, 舩越建, 加藤達也, 岡部圭介, 小林秀, 天谷雅行, 山上淳

臨床皮膚科 ((株)医学書院) 75 ( 4 ) 285 - 289 2021.04

ISSN 0021-4973

＜文献概要＞60歳,男性.ワルファリンカリウム内服中.初診1ヵ月前に左足外顆の皮下腫瘤を自覚した.初診2週間前に近医を受診し,ガングリオン疑いで穿刺された.その後,皮下腫瘤は増大傾向となり,疼痛も出現したため前医整形外科を受診した.MRIにて陳旧性血腫を認め,当科紹介受診した.初診時,左足外顆に径5cm大,びらんを伴う暗紫紅色の皮下腫瘤を認め,発赤と腫脹を伴っていた.造影CTで活動性出血を示唆する所見なく,皮下血腫の二次感染を疑い抗生剤投与と圧迫療法を開始したが増悪傾向で第7病日に局所麻酔下で血腫除去術を施行した.術中,深部動脈からの活動性出血を認め結紮処理した.脂肪組織と筋膜の間に血腫を生じており,自験例をdeep dissecting hematomaと診断した.血腫が出血源を圧迫していたため出血が持続的ながら緩徐であり,画像検査で活動性出血を同定できなかったと考えられた.臨床的に血腫の拡大を疑う場合は,画像所見にかかわらず早期の外科的治療介入が重要と考えた.

Streptococcus pyogenes upregulates arginine catabolism to exert its pathogenesis on the skin surface

Hirose Y., Yamaguchi M., Sumitomo T., Nakata M., Hanada T., Okuzaki D., Motooka D., Mori Y., Kawasaki H., Coady A., Uchiyama S., Hiraoka M., Zurich R.H., Amagai M., Nizet V., Kawabata S.

Cell Reports (Cell Reports) 34 ( 13 ) 2021.03

The arginine deiminase (ADI) pathway has been found in many kinds of bacteria and functions to supplement energy production and provide protection against acid stress. The Streptococcus pyogenes ADI pathway is upregulated upon exposure to various environmental stresses, including glucose starvation. However, there are several unclear points about the advantages to the organism for upregulating arginine catabolism. We show that the ADI pathway contributes to bacterial viability and pathogenesis under low-glucose conditions. S. pyogenes changes global gene expression, including upregulation of virulence genes, by catabolizing arginine. In a murine model of epicutaneous infection, S. pyogenes uses the ADI pathway to augment its pathogenicity by increasing the expression of virulence genes, including those encoding the exotoxins. We also find that arginine from stratum-corneum-derived filaggrin is a key substrate for the ADI pathway. In summary, arginine is a nutrient source that promotes the pathogenicity of S. pyogenes on the skin.

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Single nucleotide variations in genes associated with innate immunity are enriched in Japanese adult cases of face and neck type atopic dermatitis

Yasuda-Sekiguchi F., Shiohama A., Fukushima A., Obata S., Mochimaru N., Honda A., Kawasaki H., Kubo A., Ebihara T., Amagai M., Sasaki T.

Journal of Dermatological Science (Journal of Dermatological Science) 101 ( 2 ) 93 - 100 2021.02

ISSN 09231811

Background: Atopic dermatitis (AD) is heterogenous in terms of phenotype as well as genetic and environmental factors, while its associated genetic factors and pathophysiology are not fully understood. Objective: We identify novel genetic factors enriched in a subgroup of AD patients with characteristic clinical features. Methods: We clinically subgrouped 18 AD patients who exhibited distinctive characteristic of persistent skin eruption areas on the face and neck from 92 Japanese adult AD patients and identified disease-associated genetic factors enriched within the subgroup. Targeted resequencing and subsequent genetic association analyses were used to identify novel enriched genetic variations in the subgroup compared with the other AD patients. Results: Targeted resequencing of 648 skin associated genes revealed an enrichment of 12 single nucleotide variations (SNVs) in patients with face and neck AD (n = 18) compared with the general Japanese population in the database. Subsequent allele frequency comparison between the face and neck AD and non - face and neck AD subgroups revealed enrichment of five SNVs. Multivariate analysis using genotype data revealed that three SNVs in theTLR1, TIRAP, and PSAPL1 genes, two of the three genes are involved in the Toll-like receptor pathway, were significantly enriched in patients with face and neck AD. Conclusion: These findings revealed that the SNVs in genes associated with the innate immune pathway are enriched in a subgroup of AD. The combinational approach of clinical subgrouping and genotyping is valuable for detecting novel disease-associated genetic factors.

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【最近の水疱症】天疱瘡の寛解後に発症した水疱性類天疱瘡

田原海, 古市祐樹, 舩越建, 久保亮治, 天谷雅行, 山上淳

皮膚病診療 ((株)協和企画) 43 ( 2 ) 116 - 119 2021.02

ISSN 0387-7531

＜文献概要＞症例のポイント　・天疱瘡の長期寛解後に水疱性類天疱瘡(bullous pemphigoid:BP)とネフローゼ症候群を発症し,またその罹患後に間質性肺炎で死亡した1例を報告した.・同一患者に,2種類の自己免疫性水疱症が時を経て発症した.またネフローゼ症候群,間質性肺炎の発症メカニズムに,水疱性類天疱瘡と共通した自己免疫反応の存在が示唆された.詳細な関連や機構については不明な点が多く,今後のさらなる症例の蓄積が必要である.

Clinical utility of SARS-CoV-2 whole genome sequencing in deciphering source of infection

Takenouchi T., Iwasaki Y.W., Harada S., Ishizu H., Uwamino Y., Uno S., Osada A., Abe K., Hasegawa N., Murata M., Takebayashi T., Fukunaga K., Saya H., Kitagawa Y., Amagai M., Siomi H., Kosaki K.

Journal of Hospital Infection (Journal of Hospital Infection) 107 40 - 44 2021.01

ISSN 01956701

Coronavirus disease 2019 (COVID-19) caused by human severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a worldwide problem. From the standpoint of hospital infection control, determining the source of infection is critical. We conducted the present study to evaluate the efficacy of using whole genome sequencing to determine the source of infection in hospitalized patients who do not have a clear infectious contact history. Recently, we encountered two seemingly separate COVID-19 clusters in a tertiary hospital. Whole viral genome sequencing distinguished the two clusters according to the viral haplotype. However, the source of infection was unclear in 14 patients with COVID-19 who were clinically unlinked to clusters 1 or 2. These patients, who had no clear history of infectious contact within the hospital (‘undetermined source of infection’), had haplotypes similar to those in cluster 2 but did not have two of the mutations used to characterize cluster 2, suggesting that these 14 cases of ‘undetermined source of infection’ were not derived from cluster 2. Whole viral genome sequencing can be useful for confirming that sporadic COVID-19 cases with an undetermined source of infection are indeed not part of clusters at the institutional level.

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Cholesterol 25-hydroxylase is a metabolic switch to constrain T cell–mediated inflammation in the skin

Takahashi H., Nomura H., Iriki H., Kubo A., Isami K., Mikami Y., Mukai M., Sasaki T., Yamagami J., Kudoh J., Ito H., Kamata A., Kurebayashi Y., Yoshida H., Yoshimura A., Sun H.W., Suematsu M., O’Shea J.J., Kanno Y., Amagai M.

Science Immunology (Science Immunology) 6 ( 64 ) 2021

Interleukin-27 (IL-27) is an immunoregulatory cytokine whose essential function is to limit immune responses. We found that the gene encoding cholesterol 25-hydroxylase (Ch25h) was induced in CD4+ T cells by IL-27, enhanced by transforming growth factor–β (TGF-β), and antagonized by T-bet. Ch25h catalyzes cholesterol to generate 25-hydroxycholesterol (25OHC), which was subsequently released to the cellular milieu, functioning as a modulator of T cell response. Extracellular 25OHC suppressed cholesterol biosynthesis in T cells, inhibited cell growth, and induced nutrient deprivation cell death without releasing high-mobility group box 1 (HMGB1). This growth inhibitory effect was specific to actively proliferating cells with high cholesterol demand and was reversed when extracellular cholesterol was replenished. Ch25h-expressing CD4+ T cells that received IL-27 and TGF-β signals became refractory to 25OHC-mediated growth inhibition in vitro. Nonetheless, IL-27–treated T cells negatively affected viability of bystander cells in a paracrine manner, but only if the bystander cells were in the early phases of activation. In mouse models of skin inflammation due to autoreactive T cells or chemically induced hypersensitivity, genetic deletion of Ch25h or Il27ra led to worse outcomes. Thus, Ch25h is an immunoregulatory metabolic switch induced by IL-27 and dampens excess bystander T effector expansion in tissues through its metabolite derivative, 25OHC. This study reveals regulation of cholesterol metabolism as a modality for controlling tissue inflammation and thus represents a mechanism underlying T cell immunoregulatory functions.

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Stratification of atopic dermatitis patients by patterns of response to proactive therapy with topical tacrolimus: low serum IgE levels and inadequately controlled disease activity at the start of treatment predict its failure

Kasai H., Kawasaki H., Fukushima-Nomura A., Yasuda-Sekiguchi F., Amagai M., Ebihara T., Tanese K.

Annals of Medicine (Annals of Medicine) 53 ( 1 ) 2205 - 2214 2021

ISSN 07853890

Purpose: Topical calcineurin inhibitors (TCIs) are an important anti-inflammatory drug for treating atopic dermatitis (AD). However, those treatment responses are variable. In this study, we stratified AD patients by patterns of response to remission maintenance therapy (proactive therapy) with topical tacrolimus, a typical TCI. Thereafter, we explored patient features that predict the success or failure of proactive therapy using TCI (TCI proactive therapy). Methods: A single-arm open-label clinical study aimed to evaluate the efficacy of TCI proactive therapy was conducted in 31 patients with AD. Patients were treated with TCS to induce remission (remission-induction period) followed by daily TCI ointment (0.1% tacrolimus) application for 4 weeks (maintenance therapy period), and twice-weekly application for 12 weeks (proactive therapy period). Based on its results, treatment outcomes were correlated with the patients’ clinical and laboratory findings. Results: Of the 31 patients enrolled in the study, 21 successfully completed maintenance therapy (TCI responders). Among them, 13 completed (proactive-completed group) and 8 failed proactive therapy (proactive-dropout group). At the beginning of maintenance therapy, the serum IgE level was significantly higher in the TCI responders than in those who failed maintenance therapy (p = 0.049). At the beginning of proactive therapy, the mean-SCORing Atopic Dermatitis (SCORAD) score was significantly different between the proactive-completed (11.7 ± 4.6) and proactive-dropout (16.6 ± 4.2) groups (p = 0.025). In proactive-dropout group patients, worsened disease activity correlated well with the elevation of serum lactate dehydrogenase (LDH) and Thymus and activation-regulated chemokine (TARC) levels and peripheral eosinophil count. Conclusion: AD patients were stratified into three different response patterns to TCI proactive therapy. Patients with less involvement of IgE in the pathogenesis and inadequate remission induction by TCS may not be expected to respond well to TCI proactive therapy.Key messages AD patients can be stratified into three types according to their pattern of responsiveness to TCI proactive therapy. The efficacy of TCI proactive therapy is lower in AD patients with lower serum IgE levels. TCI proactive therapy should be done after the achievement of adequate remission induction by TCS.

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Sars-cov-2 infection among medical institution faculty and healthcare workers in tokyo, japan

Nishimura T., Uwamino Y., Uno S., Kashimura S., Shiraki T., Kurafuji T., Morita M., Noguchi M., Azegami T., Yamada-Goto N., Murai-Takeda A., Yokoyama H., Kuwabara K., Kato S., Matsumoto M., Hirata A., Iida M., Harada S., Ishizaka T., Misawa K., Murata M., Saya H., Amagai M., Kitagawa Y., Takeuchi T., Mori M., Takebayashi T., Hasegawa N.

Internal Medicine (Internal Medicine) 60 ( 16 ) 2569 - 2575 2021

ISSN 09182918

Objective To consider effective measures against severe acute respiratory syndrome coronavirus 2 (SARSCoV- 2) infection in medical institutions, this study estimated the SARS-CoV-2 infection rate among healthcare workers (HCWs) in Tokyo, Japan, and determined the specific findings for mild coronavirus disease 2019 (COVID-19) cases. Methods This study analyzed the results of serologic tests to detect immunoglobulin G antibodies against SARS-CoV-2 and evaluated the demographic and clinical characteristics of the faculty and HCWs at a Tokyo medical institution in August 2020. The demographic and clinical characteristics of participants with antibody-positive results were compared to those of participants with antibody-negative results. Materials This study recruited 2,341 faculty and HCWs at a Tokyo medical institution, 21 of whom had a COVID-19 history. Results Of the 2,320 participants without a COVID-19 history, 20 (0.862%) had positive serologic test results. A fever and dysgeusia or dysosmia occurred with greater frequency among the participants with positive test results than in those with negative results [odds ratio (OR), 5.475; 95% confidence interval (CI), 1.960-15.293 and OR, 24.158; 95% CI, 2.693-216.720, respectively]. No significant difference was observed in the positivity rate between HCWs providing medical care for COVID-19 patients using adequate protection and other HCWs (OR, 2.514; 95% CI, 0.959-6.588). Conclusion To reduce the risk of COVID-19 spread in medical institutions, faculty and HCWs should follow standard and necessary transmission-based precautions, and those with a fever and dysgeusia or dysosmia should excuse themselves from work as soon as possible.

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Identification of b.1.346 lineage of sars-cov-2 in japan: Genomic evidence of re-entry of clade 20c

Abe K., Shimura T., Takenouchi T., Iwasaki Y.W., Ishizu H., Uwamino Y., Uno S., Gotoh J., Tachikawa N., Takeuchi Y., Katayama J., Nozaki H., Fujii S., Seki S., Nakamura M., Uda K., Misumi T., Ishihara J., Yamada K., Kanai T., Murai S., Araki K., Ebihara T., Siomi H., Hasegawa N., Kitagawa Y., Amagai M., Suematsu M., Kosaki K.

Keio Journal of Medicine (Keio Journal of Medicine) 70 ( 2 ) 44 - 50 2021

ISSN 00229717

SARS-CoV-2 whole-genome sequencing of samples from COVID-19 patients is useful for informing infection control. Datasets of these genomes assembled from multiple hospitals can give critical clues to regional or national trends in infection. Herein, we report a lineage summary based on data collected from hospitals located in the Tokyo metropolitan area. We performed SARS-CoV-2 whole-genome sequencing of specimens from 198 patients with COVID-19 at 13 collaborating hospitals located in the Kanto region. Phylogenetic analysis and fingerprinting of the nucleotide substitutions were performed to differentiate and classify the viral lineages. More than 90% of the identified strains belonged to Clade 20B, which has been prevalent in European countries since March 2020. Only two lineages (B.1.1.284 and B.1.1.214) were found to be predominant in Japan. However, one sample from a COVID-19 patient admitted to a hospital in the Kanto region in November 2020 belonged to the B.1.346 lineage of Clade 20C, which has been prevalent in the western United States since November 2020. The patient had no history of overseas travel or any known contact with anyone who had travelled abroad. Consequently, the Clade 20C strain belonging to the B.1.346 lineage appeared likely to have been imported from the western United States to Japan across the strict quarantine barrier. B.1.1.284 and B.1.1.214 lineages were found to be predominant in the Kanto region, but a single case of the B.1.346 lineage of clade 20C, probably imported from the western United States, was also identified. These results illustrate that a de-centralized network of hospitals offers significant advantages as a highly responsive system for monitor-ing regional molecular epidemiologic trends.

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Co-activation of macrophages and T cells contribute to chronic GVHD in human IL-6 transgenic humanised mouse model

Ono R., Watanabe T., Kawakami E., Iwasaki M., Tomizawa-Murasawa M., Matsuda M., Najima Y., Takagi S., Fujiki S., Sato R., Mochizuki Y., Yoshida H., Sato K., Yabe H., Kato S., Saito Y., Taniguchi S., Shultz L., Ohara O., Amagai M., Koseki H., Ishikawa F.

EBioMedicine (EBioMedicine) 41 584 - 596 2019.03

© 2019 The Authors Background: Graft-versus host disease (GVHD) is a complication of stem cell transplantation associated with significant morbidity and mortality. Non-specific immune-suppression, the mainstay of treatment, may result in immune-surveillance dysfunction and disease recurrence. Methods: We created humanised mice model for chronic GVHD (cGVHD) by injecting cord blood (CB)-derived human CD34 + CD38 − CD45RA − haematopoietic stem/progenitor cells (HSPCs) into hIL-6 transgenic NOD/SCID/Il2rgKO (NSG) newborns, and compared GVHD progression with NSG newborns receiving CB CD34 − cells mimicking acute GVHD. We characterised human immune cell subsets, target organ infiltration, T-cell repertoire (TCR) and transcriptome in the humanised mice. Findings: In cGVHD humanised mice, we found activation of T cells in the spleen, lung, liver, and skin, activation of macrophages in lung and liver, and loss of appendages in skin, obstruction of bronchioles in lung and portal fibrosis in liver recapitulating cGVHD. Acute GVHD humanised mice showed activation of T cells with skewed TCR repertoire without significant macrophage activation. Interpretation: Using humanised mouse models, we demonstrated distinct immune mechanisms contributing acute and chronic GVHD. In cGVHD model, co-activation of human HSPC-derived macrophages and T cells educated in the recipient thymus contributed to delayed onset, multi-organ disease. In acute GVHD model, mature human T cells contained in the graft resulted in rapid disease progression. These humanised mouse models may facilitate future development of new molecular medicine targeting GVHD.

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Rituximab therapy for refractory autoimmune bullous diseases: A multicenter, open-label, single-arm, phase 1/2 study on 10 Japanese patients

Kurihara Y., Yamagami J., Funakoshi T., Ishii M., Miyamoto J., Fujio Y., Kakuta R., Tanikawa A., Aoyama Y., Iwatsuki K., Ishii N., Hashimoto T., Nishie W., Shimizu H., Kouyama K., Amagai M.

Journal of Dermatology (Journal of Dermatology) 46 ( 2 ) 124 - 130 2019.02

ISSN 03852407

© 2018 Japanese Dermatological Association This was a multicenter study of rituximab, a chimeric monoclonal immunoglobulin G antibody directed against CD20, for the treatment of refractory autoimmune bullous diseases (pemphigus and pemphigoid). Ten patients (three with pemphigus vulgaris, six with pemphigus foliaceus and one with bullous pemphigoid) were treated with a single cycle of rituximab (four weekly infusions at a dose of 375 mg/m 2 of body surface area). The primary end-points were the number of serious adverse events and rate of complete remission at 40 weeks. Five patients (50%) achieved complete remission with minimal therapy (defined as no active lesions with lower doses of systemic corticosteroids compared to that with prednisolone 10 mg/day). Improvements in clinical scores (Pemphigus Disease Area Index) and decreases in autoantibody titers in the sera were observed in the four pemphigus patients who failed to achieve complete remission. This suggests that rituximab was effective in nine of 10 cases. Two serious adverse events (Pneumocystis carinii pneumonia and septic shock due to infectious arthritis) were observed and adequately treated with hospitalization. CD19-positive B lymphocytes in the peripheral blood decreased on day 29 following rituximab treatment, and remained at low levels throughout the observation period (280 days). Our results confirmed the efficacy of rituximab therapy for refractory autoimmune bullous diseases in Japan.

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Identification of a human papillomavirus type 58 lineage in multiple Bowen's disease on the fingers: Case report and published work review

Kobayashi K., Tanese K., Kubo A., Matsumoto-Mochimaru N., Sasaki A., Kameyama K., Amagai M., Umegaki-Arao N.

Journal of Dermatology (Journal of Dermatology) 45 ( 10 ) 1195 - 1198 2018.10

ISSN 03852407

© 2018 Japanese Dermatological Association Human papillomavirus (HPV) has been detected in some cases of Bowen's disease, particularly on the fingers and genitalia. HPV-58 is classified as a high-risk mucosal type and accounts for a high percentage of cervical cancer in Asia. Moreover, several HPV-58 lineages, including sublineage A1, have a high prevalence in Asia. However, the nature of HPV-58-associated skin cancer is still unknown. Here, we report a case of a Japanese patient with multiple Bowen's disease on the fingers. A 33-year-old man presented with multiple reddish-brown scaly plaques on his left middle finger and right ring finger. All lesions were surgically excised, and the diagnosis of Bowen's disease was made. We performed Sanger sequencing using DNA extracted from paraffin-embedded samples and identified HPV-58 sublineage A1. Additionally, we review previous reports on HPV-58-associated skin cancers, including our case, showing a high regional prevalence in Asia. Further studies would be needed to reveal the relationship between HPV-58 lineages and carcinogenesis in the skin.

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Vancomycin mediates IgA autoreactivity in drug-induced linear IgA bullous dermatosis

Yamagami, J. Nakamura, Y. Nagao, K. Funakoshi, T. Takahashi, H. Tanikawa, A. Hachiya, T. Yamamoto, T. Ishida-Yamamoto, A. Tanaka, T. Fujimoto, N. Nishigori, C. Yoshida, T. Ishii, N. Hashimoto, T. Amagai, M.

J Invest Dermatol (Journal of Investigative Dermatology) 138 ( 7 ) 1473 - 1480 2018.07

Accepted, ISSN 0022202X

© 2018 The Authors Vancomycin (VCM) is known to induce linear IgA bullous dermatosis (LAD). However, in contrast to conventional LAD, in which circulating IgA autoantibodies against basement membrane proteins are commonly detected, patient sera from VCM-induced LAD yields negative results in indirect immunofluorescence microscopy, and the targeted autoantigen remains undetermined. By using sera from a typical patient with VCM-induced LAD, we identified that co-incubation of sera with VCM resulted in linear IgA deposition at the basement membrane zone by indirect immunofluorescence. Patient sera reacted with the dermal side of 1 mol/L NaCl-split skin and with the recombinant noncollagenous (i.e., NC1) domain of type VII collagen by both immunoblot and ELISA in the presence of VCM. The investigation of an additional 13 patients with VCM-induced LAD showed that 10 out of the 14 sera (71.4%) reacted with the NC1 domain of type VII collagen by ELISA when spiked with VCM, whereas only 4 (28.6%) tested positive without it. The enhancement of reactivity to NC1 by VCM, as determined by optical density via ELISA, was observed in 10 out of the 14 sera (71.4%). These findings indicate that type VII collagen is a target autoantigen in VCM-induced LAD and that VCM mediates IgA autoreactivity against type VII collagen, providing an insight into mechanisms involved in drug-induced autoimmune disease.

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Characterization of centriole duplication in human epidermis, Bowen's disease, and squamous cell carcinoma

Watanuki, S. Fujita, H. Kouyama, K. Amagai, M. Kubo, A.

J Dermatol Sci (Journal of Dermatological Science) 91 ( 1 ) 9 - 18 2018.07

Joint Work, Accepted, ISSN 09231811

© 2018 Background: Centrosomes contain two centrioles: a pre-existing mature centriole and a newly formed immature centriole. Each centriole is duplicated once within a cell cycle, which is crucial for proper centrosome duplication and cell division. Objective: To describe the centrosome duplication cycle in human epidermis, Bowen's disease (BD), and squamous cell carcinoma (SCC). Methods: Immunofluorescent staining of centriolar proteins and Ki-67 was used to evaluate cell cycles and the number of centrioles. Centrobin and Outer dense fiber of sperm tails 2 (ODF2) were used as markers for immature and mature centrioles, respectively. Results: Normal human primary epidermal keratinocytes in a monolayered culture have one centrobin+ centriole (CTRB1+ cells) supposed in G0/G1 phases or have two centrobin+ centrioles (CTRB2+ cells) supposed in S−G2 phase. In a three-dimensional culture and in vivo human epidermis, the majority of suprabasal cells were CTRB2+ cells, in spite of their non-proliferative Ki-67− nature. The tumor mass of BD and SCC contained CTRB1+ cells and Ki-67+ proliferating and Ki-67− non-proliferative CTRB2+ cells. Clumping cells in BD had increased numbers of centrioles, with an approximate 1:1 to 2:1 ratio of centrobin+ to ODF2+ centrioles. Conclusions: The cell cycle arrest of suprabasal cells is distinct from the G0 arrest of monolayered epithelial cells. Tumor mass of BD and SCC contained non-proliferative cells with the characteristics of the suprabasal cells of normal epidermis. A constant ratio of the number of centrobin+ to ODF2+ centrioles indicates that multiple centrioles were induced by cell division failure rather than centriole overduplication in clumping cells.

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アレルギー疾患研究、臨床研究アトピー性皮膚炎と皮膚バリア障害

AMAGAI MASAYUKI

医学のあゆみ 265 ( 9 ) 812 - 817 2018.06

Joint Work

Unique clinical and serological features of bullous pemphigoid associated with dipeptidyl peptidase-4 inhibitors

Horikawa, H. Kurihara, Y. Funakoshi, T. Umegaki-Arao, N. Takahashi, H. Kubo, A. Tanikawa, A. Kodani, N. Minami, Y. Meguro, S. Itoh, H. Izumi, K. Nishie, W. Shimizu, H. Amagai, M. Yamagami, J.

Br J Dermatol 178 ( 6 ) 1462 - 1463 2018.06

Accepted

Combination of low-dose total skin electron beam therapy and subsequent localized skin electron beam therapy as a therapeutic option for advanced-stage mycosis fungoides

Kinoshita-Ise, M. Ouchi, T. Izumi, E. Kawaguchi, O. Nagao, K. Amagai, M. Funakoshi, T.

Clinical and Experimental Dermatology 43 ( 4 ) 437 - 440 2018.06

Joint Work, Accepted

Assessment of the methods used to detect HER2-positive advanced extramammary Paget's disease

Hirai, I. Tanese, K. Nakamura, Y. Otsuka, A. Fujisawa, Y. Yamamoto, Y. Hata, H. Fujimura, T. Matsushita, S. Yoshino, K. Kameyama, K. Amagai, M. Funakoshi, T.

Medical Oncology (Medical Oncology) 35 ( 6 ) online1 - 8 2018.06

Joint Work, Accepted, ISSN 13570560

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The human epidermal growth factor receptor 2 (HER2) is recognized as an oncogene as well as a therapeutic target in various cancers. Certain patients with advanced extramammary Paget’s disease (EMPD) have also been reported to express HER2, which is therefore considered a therapeutic target for EMPD. However, an accurate methodology to determine HER2-positive EMPD has not been established. To assess the optimal methods for detection of HER2-positive EMPD, 73 EMPD samples were analyzed by immunohistochemical (IHC) staining, fluorescence in situ hybridization (FISH), and the HER2 testing algorithm for breast cancer of the American Society of Clinical Oncology/College of American Pathologists, which combined the results of IHC staining and FISH. The results showed discordance in the rate of positive IHC staining and FISH results. While 68.6% (24/35) of the metastatic samples showed equivocal or positive IHC staining, only 37.1% (13/35) were positive by FISH. To assess the accuracy of these methods, the degree of HER2 expression detected by each method was correlated with the staining profiles of activated downstream signaling pathways involving phosphorylated p44/42 MAPK (Thr202/Tyr204) (p-ERK1/2) and phosphorylated AKT (Ser473) (p-AKT). Among 16 lymph node metastasis samples, all HER2-positive samples as determined by the testing algorithm stained positively for both p-ERK1/2 and p-AKT. On the other hand, 10-14.3% of the samples determined by FISH or IHC showed negative staining for p-ERK1/2 and p-AKT. The results showed that combining the results of IHC and FISH according to the HER2 testing algorithm is a useful method for accurately evaluating HER2-positive EMPD.

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女性化乳房様を呈し、皮膚悪性腫瘍を疑った嚢胞内乳癌の男性例

AMAGAI MASAYUKI

皮膚科の臨床 60 ( 5 ) 726 - 727 2018.05

Joint Work

Bone Marrow-Derived Stem Cells Migrate into Intraepidermal Skin Defects of a Desmoglein-3 Knockout Mouse Model but Preserve their Mesodermal Differentiation

Hunefeld, C. Mezger, M. Muller-Hermelink, E. Schaller, M. Muller, I. Amagai, M. Handgretinger, R. Rocken, M.

J Invest Dermatol 138 ( 5 ) 1157 - 1165 2018.05

Joint Work, Accepted

A familial case of nail patella syndrome with a heterozygous in-frame indel mutation in the LIM domain of LMX1B

Mukai, M. Fujita, H. Umegaki-Arao, N. Sasaki, T. Yasuda-Sekiguchi, F. Isojima, T. Kitanaka, S. Amagai, M. Kubo, A.

J Dermatol Sci 90 ( 1 ) 90 - 93 2018.04

Accepted

Azathioprine monotherapy in autoimmune blistering diseases: A feasible option for mild to moderate cases

Kakuta, R. Yamagami, J. Funakoshi, T. Takahashi, H. Ohyama, M. Amagai, M.

J Dermatol (Journal of Dermatology) 45 ( 3 ) 334 - 339 2018.03

Accepted, ISSN 03852407

© 2017 Japanese Dermatological Association Azathioprine (AZP) is used as a corticosteroid (CS)-sparing medication to treat autoimmune blistering diseases. In this study, we examined the efficacy of AZP and the feasibility of using AZP monotherapy (without CS) to treat pemphigus and pemphigoid. We performed a retrospective study of 10 Japanese patients (seven with pemphigus and three with pemphigoid) with mild to moderate disease activity who had been treated using AZP. The treatment efficacy was evaluated based on decreases in the disease activity scores and autoantibody titers. The results demonstrate that seven out of 10 cases (70%) were treated successfully using AZP monotherapy with no severe adverse effects. The disease activity scores of the successfully-treated patients decreased to zero after 1–37.5 months (average, 11.9) and the average disease activity scores in these cases decreased significantly at 2 months (38.2 ± 36.6%) compared with the scores of the three patients who required additional systemic CS therapy (77.5 ± 3.5%) (P < 0.05). Additionally, the autoantibody titers of five cases treated successfully using AZP decreased by half at 6 months. In conclusion, our findings suggest that AZP monotherapy is a viable treatment option for mild to moderate pemphigus and pemphigoid.

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A Life Full of Surprises and Excitement through Science in Dermatology

AMAGAI MASAYUKI

J Invest Dermatol 138 ( 3 ) 475 - 476 2018.03

Accepted

Case of lamotrigine-induced drug adverse reaction under tocilizumab treatment with clinical and virological features of drug-induced hypersensitivity syndrome

Iriki, H. Ouchi, T. Ito, H. Sawada, M. Mukai, M. Nomura, H. Baba, Y. Adachi, T. Funakoshi, T. Amagai, M. Takahashi, H.

J Dermatol (Journal of Dermatology) 45 ( 6 ) 738 - 741 2018.03

Accepted, ISSN 03852407

© 2018 Japanese Dermatological Association The pathological mechanisms and immunological kinetics of drug-induced hypersensitivity syndrome (DIHS), including the relevance of interleukin (IL)-6, remain unclear. We report a case of drug adverse reaction that does not fulfill the diagnostic criteria of DIHS but mimics its characteristic features. Because the patient was under anti-IL-6 therapy at the onset, some symptoms typically seen in DIHS were absent, such as fever and leukocyte count abnormalities. However, the characteristic features of DIHS were clearly observed in the subsequent course, including the repeated recurrence of skin rash, prolonged liver dysfunction and reactivation of herpes viruses. This case suggested that IL-6 role at the onset is not a main factor to determine the subsequent pathomechanism of DIHS and attention should be paid to the preceding therapy for achieving accurate diagnosis.

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タンポンの使用に起因したtoxic shock syndrome

AMAGAI MASAYUKI

皮膚病診療 40 ( 2 ) 193 - 196 2018.02

Research paper (scientific journal), Joint Work

Authors' reply: Paraneoplastic autoimmune multi-organ syndrome is a distinct entity from traditional pemphigus subtypes

Payne, A. S. Kasperkiewicz, M. Ellebrecht, C. T. Takahashi, H. Yamagami, J. Zillikens, D. Amagai, M.

Nat Rev Dis Primers 4 18013 2018.02

Accepted

Diagnosis and Management of Pemphigus: recommendations by an International Panel of Experts

Murrell, D. F. Pena, S. Joly, P. Marinovic, B. Hashimoto, T. Diaz, L. A. Sinha, A. A. Payne, A. S. Daneshpazhooh, M. Eming, R. Jonkman, M. F. Mimouni, D. Borradori, L. Kim, S. C. Yamagami, J. Lehman, J. S. Saleh, M. A. Culton, D. A. Czernik, A. Zone, J. J. Fivenson, D. Ujiie, H. Wozniak, K. Akman-Karakas, A. Bernard, P. Korman, N. J. Caux, F. Drenovska, K. Prost-Squarcioni, C. Vassileva, S. Feldman, R. J. Cardones, A. R. Bauer, J. Ioannides, D. Jedlickova, H. Palisson, F. Patsatsi, A. Uzun, S. Yayli, S. Zillikens, D. Amagai, M. Hertl, M. Schmidt, E. Aoki, V. Grando, S. A. Shimizu, H. Baum, S. Cianchini, G. Feliciani, C. Iranzo, P. Mascaro, J. M., Jr. Kowalewski, C. Hall, R. Groves, R. Harman, K. E. Marinkovich, M. P. Maverakis, E. Werth, V. P.

J Am Acad Dermatol e-pub ahead of print 2018.02

Accepted

重症感染症を転機に軽快した尋常性天疱瘡

AMAGAI MASAYUKI

皮膚病診療 40 ( 1 ) 15 - 18 2018.01

Research paper (scientific journal), Joint Work

Langerhans Cells Prevent Autoimmunity via Expansion of Keratinocyte Antigen-Specific Regulatory T Cells

Kitashima, D. Y. Kobayashi, T. Woodring, T. Idouchi, K. Doebel, T. Voisin, B. Adachi, T. Ouchi, T. Takahashi, H. Nishifuji, K. Kaplan, D. H. Clausen, B. E. Amagai, M. Nagao, K.

EBioMedicine (EBioMedicine) 27 293 - 303 2018.01

Accepted

© 2017 Langerhans cells (LCs) are antigen-presenting cells in the epidermis whose roles in antigen-specific immune regulation remain incompletely understood. Desmoglein 3 (Dsg3) is a keratinocyte cell-cell adhesion molecule critical for epidermal integrity and an autoantigen in the autoimmune blistering disease pemphigus. Although antibody-mediated disease mechanisms in pemphigus are extensively characterized, the T cell aspect of this autoimmune disease still remains poorly understood. Herein, we utilized a mouse model of CD4+ T cell-mediated autoimmunity against Dsg3 to show that acquisition of Dsg3 and subsequent presentation to T cells by LCs depended on the C-type lectin langerin. The lack of LCs led to enhanced autoimmunity with impaired Dsg3-specific regulatory T cell expansion. LCs expressed the IL-2 receptor complex and the disruption of IL-2 signaling in LCs attenuated LC-mediated regulatory T cell expansion in vitro, demonstrating that direct IL-2 signaling shapes LC function. These data establish that LCs mediate peripheral tolerance against an epidermal autoantigen and point to langerin and IL-2 signaling pathways as attractive targets for achieving tolerogenic responses particularly in autoimmune blistering diseases such as pemphigus.

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Mechanisms Causing Loss of Keratinocyte Cohesion in Pemphigus

Spindler, V. Eming, R. Schmidt, E. Amagai, M. Grando, S. Jonkman, M. F. Kowalczyk, A. P. Muller, E. J. Payne, A. S. Pincelli, C. Sinha, A. A. Sprecher, E. Zillikens, D. Hertl, M. Waschke, J.

J Invest Dermatol (Journal of Investigative Dermatology) 138 ( 1 ) 32 - 37 2018.01

Accepted, ISSN 0022202X

© 2017 The Authors The autoimmune blistering skin disease pemphigus is caused by IgG autoantibodies against desmosomal cadherins, but the precise mechanisms are in part a matter of controversial discussions. This review focuses on the currently existing models of the disease and highlights the relevance of desmoglein-specific versus nondesmoglein autoantibodies, the contribution of nonautoantibody factors, and the mechanisms leading to cell dissociation and blister formation in response to autoantibody binding. As the review brings together the majority of laboratories currently working on pemphigus pathogenesis, it aims to serve as a solid basis for further investigations for the entire field.

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Combination of low-dose total skin electron beam therapy and subsequent localized skin electron beam therapy as a therapeutic option for advanced-stage mycosis fungoides

Kinoshita-Ise, M. Ouchi, T. Izumi, E. Kawaguchi, O. Nagao, K. Amagai, M. Funakoshi, T.

Clin Exp Dermatol e-pub ahead of print 2017.12

Accepted

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Association study of childhood food allergy with genome-wide association studies-discovered loci of atopic dermatitis and eosinophilic esophagitis

Hirota, T. Nakayama, T. Sato, S. Yanagida, N. Matsui, T. Sugiura, S. Takaoka, Y. Hizawa, N. Fujieda, S. Miyatake, A. Sasaki, T. Amagai, M. Doi, S. Ito, K. Ebisawa, M. Tamari, M.

J Allergy Clin Immunol 140 ( 6 ) 1713 - 1716 2017.12

Accepted

Bone Marrow-Derived Stem Cells Migrate into Intra-epidermal Skin Defects of a Desmoglein-3 knockout Mouse Model but Preserve their Mesodermal Differentiation

Hunefeld, C. Mezger, M. Muller-Hermelink, E. Schaller, M. Muller, I. Amagai, M. Handgretinger, R. Rocken, M.

J Invest Dermatol e-pub ahead of print 2017.12

Accepted

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E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning

Rubsam, M. Mertz, A. F. Kubo, A. Marg, S. Jungst, C. Goranci-Buzhala, G. Schauss, A. C. Horsley, V. Dufresne, E. R. Moser, M. Ziegler, W. Amagai, M. Wickstrom, S. A. Niessen, C. M.

Nat Commun 8 ( 1 ) 1250 2017.11

Accepted

Dermoscopy of pigmented invasive ductal carcinoma mimicking basal cell carcinoma

Horikawa, H. Umegaki-Arao, N. Funakoshi, T. Amagai, M. Tanaka, M.

Australas J Dermatol 58 ( 4 ) 326 - 327 2017.11

Accepted

Endoscopic findings of laryngopharyngeal and esophageal involvement in autoimmune bullous disease

Nakamura, R. Omori, T. Suda, K. Wada, N. Kawakubo, H. Takeuchi, H. Yamagami, J. Amagai, M. Kitagawa, Y.

Dig Endosc (Gastroenterological Endoscopy) 29 ( 7 ) 765 - 772 2017.11

Accepted, ISSN 03871207

© 2018 Japan Gastroenterological Endoscopy Society. All Rights Reserved. Background and Aim: Autoimmune bullous disease (ABD) is induced by autoantibodies against cell adhesion molecules, and blistering may occur on the mucous membranes of the eyes, nose, mouth, oral cavity, laryngopharynx, and esophagus. Endoscopic prevalence and features of ABD-associated esophageal lesions are not well known. We conducted the present study to assess the endoscopic prevalence of ABD-associated mucosal lesions. Methods: Endoscopic prevalence of mucosal lesions, particularly laryngopharyngeal and esophageal lesions, was used as the primary endpoint to assess the significance of upper gastrointestinal endoscopy, and clinical and endoscopic features were secondary endpoints. Results: Of 123 ABD patients, 50.4％ had apparent oral or laryngopharyngeal lesions and 30.8％had laryngopharyngeal lesions. Esophageal lesions were detected through normal observation in 16.8％ of affected patients, whereas 40.6％ exhibited epidermolysis or blood blisters by mechanical inducement, regardless of esophageal mucosal lesion detection by normal observation. Additionally, 56.0％ exhibited the Nikolsky sign with mechanical inducement. Of the 123 patients, 29.2％ did not have exposed skin lesions. Of these patients, 77.7％ had oral cavity or laryngopharyngeal lesions, 36.1％ had esophageal lesions, and 58.3％ exhibited the Nikolsky sign on esophageal mucosa. Conclusion: It is important to determine the endoscopic characteristics and findings of ABD. ABD can be suspected from endoscopic findings.

インフリキシマブ投与開始後に自己免疫性肝炎を発症した尋常性乾癬の1例

AMAGAI MASAYUKI

皮膚科の臨床 59 ( 11 ) 1721 - 1724 2017.10

Research paper (scientific journal), Joint Work

悪性黒色腫に対する腫瘍浸潤リンパ球(TIL)輸注療法のfeasibility試験

AMAGAI MASAYUKI

Skin Cancer 32 ( 2 ) 143 - 147 2017.10

Research paper (scientific journal), Joint Work

水疱症の新しい治療

AMAGAI MASAYUKI

皮膚科の臨床 59 ( 11 ) 1621 - 1628 2017.10

Research paper (scientific journal), Joint Work

Inflammation-free, gas-permeable, lightweight, stretchable on-skin electronics with nanomeshes

Miyamoto, Akihito, Lee, Sungwon, Cooray, Nawalage Florence, Lee, Sunghoon, Mori, Mami, Matsuhisa, Naoji, Jin, Hnbit, Yoda, Leona, Yokota, Tomoyuki, Itoh, Akira, Sekino, Masaki, Kawasaki, Hiroshi, Ebihara, Tamotsu, Amagai, Masayuki, Someya, Takao

NATURE NANOTECHNOLOGY 12 ( 9 ) 907 - + 2017.09

Research paper (scientific journal), ISSN 1748-3387

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Case of primary cutaneous methotrexate-associated Hodgkin's lymphoma

Baba, Yuko, Tanese, Keiji, Amagai, Masayuki, Kameyama, Kaori, Saito, Masataka

JOURNAL OF DERMATOLOGY 44 ( 9 ) 1079 - 1081 2017.09

Research paper (scientific journal), ISSN 0385-2407

Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice

Hayashi, Atsushi, Mikami, Yohei, Miyamoto, Kentaro, Kamada, Nobuhiko, Sato, Toshiro, Mizuno, Shinta, Naganuma, Makoto, Teratani, Toshiaki, Aoki, Ryo, Fukuda, Shinji, Suda, Wataru, Hattori, Masahira, Amagai, Masayuki, Ohyama, Manabu, Kanai, Takanori

CELL REPORTS 20 ( 7 ) 1513 - 1524 2017.08

Research paper (scientific journal), ISSN 2211-1247

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Peptide-pulsed dendritic cell vaccine in combination with carboplatin and paclitaxel chemotherapy for stage IV melanoma

Fukuda, Keitaro, Funakoshi, Takeru, Sakurai, Toshiharu, Nakamura, Yoshio, Mori, Mariko, Tanese, Keiji, Tanikawa, Akiko, Taguchi, Junichi, Fujita, Tomonobu, Okamoto, Masato, Amagai, Masayuki, Kawakami, Yutaka

MELANOMA RESEARCH 27 ( 4 ) 326 - 334 2017.08

Research paper (scientific journal), ISSN 0960-8931

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Non-pigmenting fixed drug eruption with mixed features of acute generalized exanthematous pustulosis induced by pseudoephedrine: a case report

Fukuda, Risa, Ouchi, Takeshi, Hirai, Ikuko, Funakoshi, Takeru, Honda, Aki, Tanese, Keiji, Tanikawa, Akiko, Amagai, Masayuki, Takahashi, Hayato

CONTACT DERMATITIS 77 ( 2 ) 123 - 126 2017.08

Research paper (scientific journal), ISSN 0105-1873

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Expression of monoacylglycerol lipase as a marker of tumour invasion and progression in malignant melanoma

Baba, Y. Funakoshi, T. Mori, M. Emoto, K. Masugi, Y. Ekmekcioglu, S. Amagai, M. Tanese, K.

J Eur Acad Dermatol Venereol 31 ( 2 ) 2038 - 2045 2017.07

Joint Work, Accepted

Unexpected recalcitrant course of drug-induced erythema multiforme-like eruption and interstitial pneumonia sequentially occurring after nivolumab therapy

Nomura, Hisashi, Takahashi, Hayato, Suzuki, Satsuki, Kurihara, Yuichi, Chubachi, Shotaro, Kawada, Ichiro, Yasuda, Hiroyuki, Betsuyaku, Tomoko, Amagai, Masayuki, Funakoshi, Takeru

JOURNAL OF DERMATOLOGY 44 ( 7 ) 818 - 821 2017.07

Research paper (scientific journal), ISSN 0385-2407

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Successful treatment with bendamustine and rituximab for paraneoplastic pemphigus

Kikuchi, Taku, Mori, Takehiko, Shimizu, Takayuki, Koda, Yuya, Abe, Ryohei, Kurihara, Yuichi, Funakoshi, Takeru, Yamagami, Jun, Sato, Hidekazu, Tsunoda, Kazuyuki, Amagai, Masayuki, Okamoto, Shinichiro

ANNALS OF HEMATOLOGY 96 ( 7 ) 1221 - 1222 2017.07

Research paper (scientific journal), ISSN 0939-5555

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Novel nonsense mutation in SERPINB7 and the treatment of foot odor in a patient with Nagashima-type palmoplantar keratosis

Katsuno, Masako, Shiohama, Aiko, Aoki, Satomi, Kitamura, Hajime, Sasaki, Tetsuo, Amagai, Masayuki, Kubo, Akiharu

JOURNAL OF DERMATOLOGY 44 ( 7 ) E146 - E147 2017.07

Research paper (scientific journal), ISSN 0385-2407

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日本皮膚科学会ガイドライン　類天疱瘡(後天性表皮水疱症を含む)診療ガイドライン

AMAGAI MASAYUKI

日本皮膚科学会雑誌 127 ( 7 ) 1483 - 1521 2017.06

Research paper (scientific journal), Joint Work

Somatic HRAS p.G12S mosaic mutation causes unilaterally distributed epidermal nevi, woolly hair and palmoplantar keratosis

Honda, Aki, Umegaki-Arao, Noriko, Sasaki, Takashi, Nakabayashi, Kazuhiko, Hata, Kenichiro, Matsubara, Yoichi, Tanikawa, Akiko, Amagai, Masayuki, Kubo, Akiharu

JOURNAL OF DERMATOLOGY 44 ( 6 ) E109 - E110 2017.06

Research paper (scientific journal), ISSN 0385-2407

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Lack of XAGE-1b and NY-ESO-1 in metastatic lymph nodes may predict the potential survival of stage III melanoma patients

Mori, Mariko, Funakoshi, Takeru, Kameyama, Kaori, Kawakami, Yutaka, Sato, Eiichi, Nakayama, Eiichi, Amagai, Masayuki, Tanese, Keiji

JOURNAL OF DERMATOLOGY 44 ( 6 ) 671 - 680 2017.06

Research paper (scientific journal), ISSN 0385-2407

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Meeting Report of the Pathogenesis of Pemphigus and Pemphigoid Meeting in Munich, September 2016.

Schmidt, E. Spindler, V. Eming, R. Amagai, M. Antonicelli, F. Baines, J. F. Belheouane, M. Bernard, P. Borradori, L. Caproni, M. Di Zenzo, G. Grando, S. Harman, K. Jonkman, M. F. Koga, H. Ludwig, R. J. Kowalczyk, A. P. Müller, E. J. Nishie, W. Pas, H. Payne, A. S. Sadik, C. D. Seppänen, A. Setterfield, J. Shimizu, H. Sinha, A. A. Sprecher, E. Sticherling, M. Ujiie, H. Zillikens, D. Hertl, M. Waschke, J.

J Invest Dermatol 137 ( 6 ) 1199 - 1203 2017.06

腫瘍随伴性天疱瘡6例の口腔症状に関する臨床的検討

AMAGAI MASAYUKI

日本口腔内科学会雑誌 23 ( 1 ) 1 - 8 2017.06

Research paper (scientific journal), Joint Work

特集：医学部開設100年次の100年に向けて未来の医学を語る

AMAGAI MASAYUKI

三田評論 1211 10 - 26 2017.05

Joint Work, Accepted

天疱瘡　ーデスモグレインに対する細胞性免疫とバリア機能障害

AMAGAI MASAYUKI

実験医学 35 ( 7（増刊） ) 101 - 107 2017.05

Research paper (scientific journal), Joint Work, Accepted

GVHD-like erythroderma in the clinical course of thymoma-associated myasthenia gravis

Nomura, Hisashi, Suzuki, Shigeaki, Yasuda-Sekiguchi, Fumiyo, Amagai, Masayuki, Yamada, Satoshi, Nakahara, Jin, Suzuki, Norihiro, Kohno, Mitsutomo, Funakoshi, Takeru

EUROPEAN JOURNAL OF DERMATOLOGY 27 ( 3 ) 314 - 315 2017.05

Research paper (scientific journal), ISSN 1167-1122

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Pemphigus

Kasperkiewicz, Michael, Ellebrecht, Christoph T., Takahashi, Hayato, Yamagami, Jun, Zillikens, Detlef, Payne, Aimee S., Amagai, Masayuki

NATURE REVIEWS DISEASE PRIMERS 3 E109 - E110 2017.05

Research paper (scientific journal), ISSN 2056-676X

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Distinct phenotype of epidermolysis bullosa simplex with infantile migratory circinate erythema due to frameshift mutations in the V2 domain of KRT5

Kumagai, Y., Umegaki-Arao, N., Sasaki, T., Nakamura, Y., Takahashi, H., Ashida, A., Tsunemi, Y., Kawashima, M., Shimizu, A., Ishiko, A., Nakamura, K., Tsuchihashi, H., Amagai, M., Kubo, A.

JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY 31 ( 5 ) e241 - e243 2017.05

Research paper (scientific journal), ISSN 0926-9959

Dissecting the formation, structure and barrier function of the stratum corneum (vol 27, pg 269, 2015)

Matsui, Takeshi, Amagai, Masayuki

INTERNATIONAL IMMUNOLOGY 29 ( 5 ) 243 - 244 2017.05

Research paper (scientific journal), ISSN 0953-8178

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腎移植後の免疫抑制患者に生じた巨大尖圭コンジローマの1例

AMAGAI MASAYUKI

臨床皮膚科 71 ( 6 ) 463 - 468 2017.05

Research paper (scientific journal), Joint Work, Accepted

臨床医学における基礎研究

AMAGAI MASAYUKI

科研費NEWS 2 7 2017.05

Single Work, Accepted

アトピー性皮膚炎と　バリアケア

AMAGAI MASAYUKI

DERMATOLOGY CLUB 4 1 - 4 2017.04

Single Work

Inflammatory Linear Verrucous Epidermal Nevus with a Postzygotic GJA1 Mutation Is a Mosaic Erythrokeratodermia Variabilis et Progressiva

Umegaki-Arao, Noriko, Sasaki, Takashi, Fujita, Harumi, Aoki, Satomi, Kameyama, Kaori, Amagai, Masayuki, Seishima, Mariko, Kubo, Akiharu

JOURNAL OF INVESTIGATIVE DERMATOLOGY 137 ( 4 ) 967 - 970 2017.04

Research paper (scientific journal), ISSN 0022-202X

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自己免疫疾患・膠原病・血管炎など　天疱瘡・類天疱瘡を疑う食道病変

AMAGAI MASAYUKI

消化器内視鏡 29 ( 4 ) 716 - 718 2017.04

Research paper (scientific journal), Joint Work, Accepted

Non-pathogenic pemphigus foliaceus (PF) IgG acts synergistically with a directly pathogenic PF IgG to increase blistering by p38MAPK-dependent desmoglein 1 clustering

Yoshida, K. Ishii, K. Shimizu, A. Yokouchi, M. Amagai, M. Shiraishi, K. Shirakata, Y. Stanley, J. R. Ishiko, A.

J Dermatol Sci 85 ( 3 ) 197 - 207 2017.03

Research paper (scientific journal), Joint Work, Accepted

Validation of chemiluminescent enzyme immunoassay in detection of autoantibodies in pemphigus and pemphigoid

Fujio, Y. Kojima, K. Hashiguchi, M. Wakui, M. Murata, M. Amagai, M. Yamagami, J.

J Dermatol Sci 85 ( 3 ) 208 - 215 2017.03

Research paper (scientific journal), Joint Work, Accepted

Induction of hair follicle dermal papilla cell properties in human induced pluripotent stem cell-derived multipotent LNGFR(+) THY-1(+) mesenchymal cells

Veraitch, O. Mabuchi, Y. Matsuzaki, Y. Sasaki, T. Okuno, H. Tsukashima, A. Amagai, M. Okano, H. Ohyama, M.

Scientific Reports 7 2017.02

Research paper (scientific journal), Joint Work, Accepted

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経過中に抗デスモグレイン抗体陽性となり診断しえた腫瘍随伴性天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 71 ( 2 ) 104 - 110 2017.02

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

A randomized double-blind trial of intravenous immunoglobulin for bullous pemphigoid

Amagai, M. Ikeda, S. Hashimoto, T. Mizuashi, M. Fujisawa, A. Ihn, H. Matsuzaki, Y. Ohtsuka, M. Fujiwara, H. Furuta, J. Tago, O. Yamagami, J. Tanikawa, A. Uhara, H. Morita, A. Nakanishi, G. Tani, M. Aoyama, Y. Makino, E. Muto, M. Manabe, M. Konno, T. Murata, S. Izaki, S. Watanabe, H. Yamaguchi, Y. Matsukura, S. Seishima, M. Habe, K. Yoshida, Y. Kaneko, S. Shindo, H. Nakajima, K. Kanekura, T. Takahashi, K. Kitajima, Y. Hashimoto, K. Bullous Pemphigoid Study, Group

J Dermatol Sci 85 ( 2 ) 77 - 84 2017.02

Research paper (scientific journal), Joint Work, Accepted

Bullous pemphigoid in infancy with spontaneous remission

Nomura, H. Funakoshi, T. Baba, A. Tanikawa, A. Hayakawa, K. Amagai, M.

Eur J Dermatol 27 ( 1 ) 95 - 96 2017.02

Research paper (scientific journal), Joint Work, Accepted

Case of dominant dystrophic epidermolysis bullosa with amniotic band syndrome

Sakiyama, T. Umegaki-Arao, N. Sasaki, T. Kosaki, K. Amagai, M. Kubo, A.

J Dermatol 44 ( 1 ) 102 - 103 2017.01

Research paper (scientific journal), Joint Work, Accepted

アトピー性皮膚炎のリバーストランスレーショナルリサーチ

AMAGAI MASAYUKI

実験医学 35 ( 1 ) 33 - 39 2017.01

Research paper (scientific journal), Joint Work, Accepted

Increasing Comorbidities Suggest that Atopic Dermatitis Is a Systemic Disorder

Brunner, P. M. Silverberg, J. I. Guttman-Yassky, E. Paller, A. S. Kabashima, K. Amagai, M. Luger, T. A. Deleuran, M. Werfel, T. Eyerich, K. Stingl, G. Councilors of the International Eczema, Council

J Invest Dermatol 137 ( 1 ) 18 - 25 2017.01

Research paper (scientific journal), Joint Work, Accepted

Identification of factors contributing to phenotypic divergence via quantitative image analyses of autosomal recessive woolly hair/hypotrichosis with homozygous c.736T>A LIPH mutation

Kinoshita-Ise, M. Kubo, A. Sasaki, T. Umegaki-Arao, N. Amagai, M. Ohyama, M.

Br J Dermatol 176 ( 1 ) 138 - 144 2017.01

Research paper (scientific journal), Joint Work, Accepted

Evaluation of pharyngo-oesophageal involvement in pemphigus vulgaris and its correlation with disease activity

Okamura, A. Nakamura, R. Yamagami, J. Ishii, K. Kawakubo, H. Omori, T. Takeuchi, H. Amagai, M. Kitagawa, Y.

Br J Dermatol 176 ( 1 ) 224 - 226 2017.01

Research paper (scientific journal), Joint Work, Accepted

関節リウマチ患者に生じたメトトレキサート関連リンパ増殖性疾患と考えられた1例

AMAGAI MASAYUKI

臨床皮膚科 70 ( 13 ) 1067 - 1071 2016.12

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Epidermal cell turnover across tight junctions based on Kelvin's tetrakaidecahedron cell shape

Yokouchi, M. Atsugi, T. Logtestijn, M. V. Tanaka, R. J. Kajimura, M. Suematsu, M. Furuse, M. Amagai, M. Kubo, A.

eLife 5 e19593 2016.11

Research paper (scientific journal), Joint Work, Accepted

多彩な皮疹を呈し尋常性乾癬として加療されていた落葉状天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 70 ( 12 ) 949 - 954 2016.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

天疱瘡と類天疱瘡

AMAGAI MASAYUKI

JOHNS 32 ( 11 ) 1601 - 1604 2016.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

増殖性天疱瘡　臨床像、病理組織像の相関性

AMAGAI MASAYUKI

皮膚病診療 38 ( 11 ) 1121 - 1124 2016.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

簡便かつ有用な巻き爪の評価法

AMAGAI MASAYUKI

日本皮膚科学会雑誌 126 ( 12 ) 2275 - 2280 2016.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

乳癌の放射線治療部位に再燃した落葉状天疱瘡

AMAGAI MASAYUKI

皮膚病診療 38 ( 11 ) 1093 - 1096 2016.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

原因薬剤の中止により軽快したブシラミン誘発性疱疹状天疱瘡

AMAGAI MASAYUKI

皮膚病診療 38 ( 11 ) 1117 - 1120 2016.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

4歳で発症し、プレドニゾロン内服治療で軽快した尋常性天疱瘡の小児例

AMAGAI MASAYUKI

皮膚病診療 38 ( 11 ) 1081 - 1084 2016.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Nivolumab for advanced melanoma: pretreatment prognostic factors and early outcome markers during therapy

Nakamura, Y. Kitano, S. Takahashi, A. Tsutsumida, A. Namikawa, K. Tanese, K. Abe, T. Funakoshi, T. Yamamoto, N. Amagai, M. Yamazaki, N.

Oncotarget 7 ( 47 ) 77404 - 77415 2016.11

Research paper (scientific journal), Joint Work, Accepted

Case of lymphoplasmacytic plaque in children: Analysis of the distribution of infiltrating immune cells

Mukai, M. Tanese, K. Umegaki-Arao, N. Kubo, A. Takahashi, H. Amagai, M.

J Dermatol 43 ( 11 ) 1368 - 1370 2016.10

Research paper (scientific journal), Joint Work, Accepted

天疱瘡におけるデスモグレイン3特異的T細胞の臓器を越えた病原的役割

AMAGAI MASAYUKI

医学のあゆみ 259 ( 2 ) 159 - 164 2016.10

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Distinct phenotype of epidermolysis bullosa simplex with infantile migratory circinate erythema due to frameshift mutations in the V2 domain of KRT5

Kumagai, Y. Umegaki-Arao, N. Sasaki, T. Nakamura, Y. Takahashi, H. Ashida, A. Tsunemi, Y. Kawashima, M. Shimizu, A. Ishiko, A. Nakamura, K. Tsuchihashi, H. Amagai, M. Kubo, A.

J Eur Acad Dermatol Venereol 2016.10

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

A case of primary cutaneous methotrexate-associated Hodgkin lymphoma

Baba, Y. Tanese, K. Amagai, M. Kameyama, K. Saito, M.

J Dermatol 2016.09

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Dermoscopic findings in pigmented Bowen's disease of the lip

Nomura, H. Funakoshi, T. Yokoyama, T. Amagai, M. Okabe, K. Kishi, K. Tanikawa, A.

Eur J Dermatol 26 ( 6 ) 613 - 614 2016.08

Research paper (scientific journal), Joint Work, Accepted

皮膚バリアの破綻とアトピー性皮膚炎

AMAGAI MASAYUKI

Medical Science Digest 42 ( 6 ) 268 - 271 2016.06

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

知っておくべき基本事項　皮膚バリアと経皮感作

AMAGAI MASAYUKI

あたらしい眼科 33 ( 3 ) 333 - 341 2016.03

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Identification and Characterization of a Recessive Missense Mutation p.P277L in SERPINB7 in Nagashima-Type Palmoplantar Keratosis

Shiohama, A. Sasaki, T. Sato, S. Sakabe, J. I. Ito, T. Isoda, H. Zenke, Y. Nakano, T. Maeda, T. Ishiko, A. Kabashima, K. Tokura, Y. Mitsuhashi, Y. Amagai, M. Kubo, A.

J Invest Dermatol 136 ( 1 ) 325 - 328 2016.01

Research paper (scientific journal), Joint Work, Accepted

Japanese recurrent mutation c.6216+5G>T in COL7A1 leads to a mild phenotype of dystrophic epidermolysis bullosa

Masunaga, T. Saito, M. Sasaki, T. Kubo, A. Amagai, M. Ishiko, A.

J Dermatol Sci 80 ( 3 ) 220 - 223 2015.12

Research paper (scientific journal), Joint Work, Accepted

Postherpetic Wolf's isotopic response: possible contribution of resident memory T cells to the pathogenesis of lichenoid reaction

Ise, M. Tanese, K. Adachi, T. Du, W. Amagai, M. Ohyama, M.

Br J Dermatol 173 ( 5 ) 1331 - 1334 2015.11

Research paper (scientific journal), Joint Work, Accepted

【マイクロバイオ−ムとウイローム】疾患との関連　皮膚疾患

AMAGAI MASAYUKI

臨床と微生物 42 ( 6 ) 717 - 721 2015.11

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Hair follicle-derived IL-7 and IL-15 mediate skin-resident memory T cell homeostasis and lymphoma

Adachi, T. Kobayashi, T. Sugihara, E. Yamada, T. Ikuta, K. Pittaluga, S. Saya, H. Amagai, M. Nagao, K.

Nat Med 21 ( 11 ) 1272 - 1279 2015.11

Research paper (scientific journal), Joint Work, Accepted

サイトメガロウイルス食道炎を併発した高齢の落葉状天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 69 ( 11 ) 821 - 826 2015.10

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Modulating Immunity to Treat Autoimmune Disease

Amagai, M.

N Engl J Med 375 ( 15 ) 1487 - 1489 2015.10

Research paper (scientific journal), Single Work, Accepted

Metastatic eccrine porocarcinoma successfully treated with carboplatin and epirubicin chemotherapy

Fukuda, K. Funakoshi, T. Fukuyama, M. Kakuta, R. Sato, M. Nakamura, Y. Tanese, K. Masugi, Y. Amagai, M.

J Dermatol 42 ( 9 ) 928 - 930 2015.09

Research paper (scientific journal), Joint Work, Accepted

Application of electron microscopic analysis and fluorescent in situ hybridization technique for the successful diagnosis of extraskeletal Ewing's sarcoma

Funakoshi, T. Sato, T. Hosokawa, R. Saito, M. Amagai, M. Ishiko, A. Ohyama, M.

J Dermatol 42 ( 9 ) 893 - 896 2015.09

Research paper (scientific journal), Joint Work, Accepted

Exploring the biology of the nail: An intriguing but less-investigated skin appendage

Saito, M. Ohyama, M. Amagai, M.

J Dermatol Sci 79 ( 3 ) 187 - 193 2015.09

Research paper (scientific journal), Joint Work, Accepted

Cracking the code of skin inflammation with CD1a

Amagai, M.

Nat Immunol 17 ( 10 ) 1133 - 1134 2015.09

Research paper (scientific journal), Single Work, Accepted

デスモグレイン3特異的T細胞によって誘導される多彩な皮膚病理

AMAGAI MASAYUKI

実験医学 33 ( 12（増刊） ) 1948 - 1953 2015.08

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Pathological characterization of pachydermia in pachydermoperiostosis

Tanese, K. Niizeki, H. Seki, A. Otsuka, A. Kabashima, K. Kosaki, K. Kuwahara, M. Miyakawa, S. I. Miyasaka, M. Matsuoka, K. Okuyama, T. Shiohama, A. Sasaki, T. Kudoh, J. Amagai, M. Ishiko, A.

J Dermatol 42 ( 7 ) 710 - 714 2015.07

Research paper (scientific journal), Joint Work, Accepted

二次性細菌感染により壊疽性膿瘡様皮疹を呈した水疱性類天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 69 ( 8 ) 554 - 559 2015.07

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Low pathogenicity of anti-desmoglein 3 immunoglobulin G autoantibodies contributes to the atypical clinical phenotypes in pemphigus

Saleh, M. A. Hashimoto, R. Kase, Y. Amagai, M. Yamagami, J.

J Dermatol 42 ( 7 ) 685 - 689 2015.07

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

皮膚バリアとランゲルハンス細胞

AMAGAI MASAYUKI

臨床免疫・アレルギー科 64 ( 1 ) 26 - 32 2015.07

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Dissecting the formation, structure, and barrier function of the stratum corneum

Matsui, T. Amagai, M.

Int Immunol 27 ( 6 ) 269 - 280 2015.06

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Periostin is a key niche component for wound metastasis of melanoma

Fukuda, K. Sugihara, E. Ohta, S. Izuhara, K. Funakoshi, T. Amagai, M. Saya, H.

PLoS One 10 ( 6 ) e0129704 2015.06

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

The International Societies for Investigative Dermatology: The Realization of a Vision for Unity and Collaboration in the Advancement of Investigative Dermatology and Cutaneous Biology

Amagai, M. Enk, A. Hall, R. P.

J Invest Dermatol 134 ( 5 ) 1181 - 1183 2015.05

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Splicing abnormality of integrin beta4 gene (ITGB4) due to nucleotide substitutions far from splice site underlies pyloric atresia-junctional epidermolysis bullosa syndrome

Masunaga, T. Niizeki, H. Yasuda, F. Yoshida, K. Amagai, M. Ishiko, A.

J Dermatol Sci 78 ( 1 ) 61 - 66 2015.04

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

頭頂部脱毛性腫瘤を呈した閉鎖性脳瘤（atretic cephalocele）の1例

AMAGAI MASAYUKI

臨床皮膚科 69 ( 4 ) 327 - 331 2015.04

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Dysbiosis and Staphyloccus aureus Colonization Drives Inflammation in Atopic Dermatitis

Kobayashi, T. Glatz, M. Horiuchi, K. Kawasaki, H. Akiyama, H. Kaplan, D. H. Kong, H. H. Amagai, M. Nagao, K.

Immunity 42 ( 4 ) 756 - 766 2015.04

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Case of angiosarcoma on the abdominal wall, an extremely rare variant, putatively shared the pathogenesis with Stewart-Treves syndrome

Ise, M. Funakoshi, T. Furuichi, Y. Honda, H. Fujio, Y. Amagai, M. Ohyama, M.

J Dermatol 42 ( 4 ) 426 - 427 2015.04

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Chronology of confluent and reticulated papillomatosis: Spontaneous regression in a case after long-term follow-up may imply transient nature of the condition

Sakiyama, T. Amagai, M. Ohyama, M.

J Dermatol 42 ( 3 ) 335 - 336 2015.03

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Message from the former president of JSID: Towards a bright future for JSID

Amagai, M.

J Dermatol Sci 77 ( 3 ) 137 - 138 2015.03

Research paper (scientific journal), Single Work, Accepted

Access to Document (DOI)

Case of non-Herlitz junctional epidermolysis bullosa with COL17A1 mutation

Kasai, H. Sasaki, T. Matsuzaki, H. Yoshioka, T. Nagao, K. Amagai, M. Ishiko, A. Kubo, A.

J Dermatol 42 ( 3 ) 323 - 325 2015.03

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

自己免疫性水疱症と上部消化管病変の内視鏡診断

AMAGAI MASAYUKI

胃と腸 50 ( 2 ) 159 - 173 2015.02

Research paper (scientific journal), Joint Work, Accepted

Preface to Journal of Dermatology special issue: pemphigus updates from around the world

Amagai, M.

J Dermatol 42 ( 1 ) 2 2015.01

Research paper (scientific journal), Single Work, Accepted

Access to Document (DOI)

Definitions and outcome measures for mucous membrane pemphigoid: recommendations of an international panel of experts

Murrell, D. F. Marinovic, B. Caux, F. Prost, C. Ahmed, R. Wozniak, K. Amagai, M. Bauer, J. Beissert, S. Borradori, L. Culton, D. Fairley, J. A. Fivenson, D. Jonkman, M. F. Marinkovich, M. P. Woodley, D. Zone, J. Aoki, V. Bernard, P. Bruckner-Tuderman, L. Cianchini, G. Venning, V. Diaz, L. Eming, R. Grando, S. A. Hall, R. P. Hashimoto, T. Herrero-Gonzalez, J. E. Hertl, M. Joly, P. Karpati, S. Kim, J. Chan Kim, S. Korman, N. J. Kowalewski, C. Lee, S. E. Rubenstein, D. R. Sprecher, E. Yancey, K. Zambruno, G. Zillikens, D. Doan, S. Daniel, B. S. Werth, V. P.

J Am Acad Dermatol 72 ( 1 ) 168 - 174 2015.01

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Epidermal tight junction barrier function is altered by skin inflammation, but not by filaggrin-deficient stratum corneum

Yokouchi, M. Kubo, A. Kawasaki, H. Yoshida, K. Ishii, K. Furuse, M. Amagai, M.

J Dermatol Sci 77 ( 1 ) 28 - 36 2015.01

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

皮膚難病への挑戦　厚労省皮膚難病研究班からの報告を含めて　天疱瘡updates　基礎と臨床

AMAGAI MASAYUKI

日本皮膚科学会雑誌 124 ( 13 ) 2457 - 2459 2014.12

Research paper (scientific journal), Single Work, Accepted

若い医師たちに紡ぐことば「なぜ成功したかって？たくさん失敗したからさ.」

AMAGAI MASAYUKI

Visual Dermatology 13 ( 12 ) 1434 2014.12

Research paper (scientific journal), Single Work, Accepted

An association study of 36 psoriasis susceptibility loci for psoriasis vulgaris and atopic dermatitis in a Japanese population

Tamari, M. Saeki, H. Hayashi, M. Umezawa, Y. Ito, T. Fukuchi, O. Nobeyama, Y. Yanaba, K. Nakagawa, H. Tsunemi, Y. Kato, T. Shibata, S. Sugaya, M. Sato, S. Tada, Y. Doi, S. Miyatake, A. Ebe, K. Noguchi, E. Fujieda, S. Ebihara, T. Amagai, M. Esaki, H. Takeuchi, S. Furue, M. Hirota, T.

J Dermatol Sci 76 ( 2 ) 156 - 157 2014.11

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

多彩な外観を呈する皮膚炎症性疾患

AMAGAI MASAYUKI

別冊Bio Clinica 3 ( 2 ) 6 - 7 2014.11

Research paper (scientific journal), Single Work, Accepted

皮膚疾患におけるT細胞の重要な役割

AMAGAI MASAYUKI

実験医学 32 ( 17 ) 2854 - 2860 2014.11

Research paper (scientific journal), Single Work, Accepted

Detailed clinicopathological characterization of progressive alopecia areata patients treated with i.v. corticosteroid pulse therapy toward optimization of inclusion criteria

Sato, M. Amagai, M. Ohyama, M.

J Dermatol 41 ( 11 ) 957 - 963 2014.11

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Grading criteria for disease severity by pemphigus disease area index

Shimizu, T. Takebayashi, T. Sato, Y. Niizeki, H. Aoyama, Y. Kitajima, Y. Iwatsuki, K. Hashimoto, T. Yamagami, J. Werth, V. P. Amagai, M. Tanikawa, A.

J Dermatol 41 ( 11 ) 969 - 973 2014.11

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

水疱性類天疱瘡に対する免疫グロブリン大量静注療法の実態調査

AMAGAI MASAYUKI

新薬と臨床 63 ( 10 ) 50 - 57 2014.10

Research paper (scientific journal), Joint Work, Accepted

尋常性天疱瘡における脱毛の本態は"hair Nikolsky現象"である−自験例に基づく発症のメカニズムとその臨床的意義についての考察−

AMAGAI MASAYUKI

日本皮膚科学会雑誌 124 ( 11 ) 2119 - 2126 2014.10

Research paper (scientific journal), Joint Work, Accepted

表皮バリア不全と経皮感作が招くアレルギー疾患

AMAGAI MASAYUKI

実験医学 33 ( 4 ) 563 - 569 2014.10

Research paper (scientific journal), Joint Work, Accepted

Application of moisturizer to neonates prevents development of atopic dermatitis

Horimukai, K. Morita, K. Narita, M. Kondo, M. Kitazawa, H. Nozaki, M. Shigematsu, Y. Yoshida, K. Niizeki, H. Motomura, K. Sago, H. Takimoto, T. Inoue, E. Kamemura, N. Kido, H. Hisatsune, J. Sugai, M. Murota, H. Katayama, I. Sasaki, T. Amagai, M. Morita, H. Matsuda, A. Matsumoto, K. Saito, H. Ohya, Y.

J Allergy Clin Immunol 134 ( 4 ) 824 - 830 2014.10

Research paper (scientific journal), Joint Work, Accepted

Filaggrin loss-of-function mutations are not a predisposing factor for atopic dermatitis in an Ishigaki Island under subtropical climate

Sasaki, T. Furusyo, N. Shiohama, A. Takeuchi, S. Nakahara, T. Uchi, H. Hirota, T. Tamari, M. Shimizu, N. Ebihara, T. Amagai, M. Furue, M. Hayashi, J. Kudoh, J.

J Dermatol Sci 76 ( 1 ) 10 - 15 2014.10

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Distinct behavior of human Langerhans cells and inflammatory dendritic epidermal cells at tight junctions in atopic dermatitis

Yoshida, K. Kubo, A. Fujita, H. Yokouchi, M. Ishii, K. Kawasaki, H. Nomura, T. Shimizu, H. Kouyama, K. Ebihara, T. Nagao, K. Amagai, M.

J Allergy Clin Immunol 134 ( 4 ) 856 - 864 2014.10

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

The complete type of pachydermoperiostosis: A novel nonsense mutation p.E141* of the SLCO2A1 gene

Niizeki, H. Shiohama, A. Sasaki, T. Seki, A. Kabashima, K. Otsuka, A. Kosaki, K. Ogo, A. Yamada, T. Miyasaka, M. Matsuoka, K. Hirakiyama, A. Okuyama, T. Matsuda, M. Nakabayashi, K. Tanese, K. Ishiko, A. Amagai, M. Kudoh, J.

J Dermatol Sci 75 ( 3 ) 193 - 195 2014.09

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Histopathological investigation of clinically non-affected perilesional scalp in alopecias detected unexpected spread of disease activities

Watanabe-Okada, E. Amagai, M. Ohyama, M.

J Dermatol 41 ( 9 ) 802 - 807 2014.09

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Response to hydroxychloroquine in Japanese patients with lupus-related skin disease using the cutaneous lupus erythematosus disease area and severity index (CLASI) (vol 24, pg 701, 2014)

Yokogawa, N. Tanikawa, A. Amagai, M. Kato, Y. Momose, Y. Arai, S. Eto, H. Ikeda, T. Furukawa, F.

Mod Rheumatol 24 ( 4 ) 701 2014.07

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

頭頂部脱毛性腫瘤を呈した閉鎖性脳瘤（atretic cephalocele）の1例

AMAGAI MASAYUKI

臨床皮膚科 68 ( 8 ) 615 - 620 2014.07

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

IV期悪性黒色腫に対するカルボプラチン+パクリタキセル併用療法の救済療法としての有効性

AMAGAI MASAYUKI

日本皮膚科学会雑誌 124 ( 8 ) 1555 - 1561 2014.07

Research paper (scientific journal), Joint Work, Accepted

Follicular microhemorrhage: A unique dermoscopic sign for the detection of coexisting trichotillomania in alopecia areata

Ise, M. Amagai, M. Ohyama, M.

J Dermatol 41 ( 6 ) 518 - 520 2014.06

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

フィラグリン（filaggrin）

AMAGAI MASAYUKI

分子消化器病 11 ( 2 ) 88 - 93 2014.06

Joint Work

食道病変を認めた類天疱瘡群の4例

AMAGAI MASAYUKI

Progress of Digestive Endoscopy 84 ( 1 ) 70 - 71 2014.06

Research paper (scientific journal), Joint Work, Accepted

臀部に暗赤色隆起性局面を呈した増殖性天疱瘡

AMAGAI MASAYUKI

皮膚病診療 36 ( 6 ) 515 - 518 2014.06

Research paper (scientific journal), Joint Work, Accepted

皮膚バリア異常を示す自然発症皮膚炎マウスモデル

AMAGAI MASAYUKI

日本臨床免疫学会会誌 37 ( 3 ) 160 - 165 2014.06

Research paper (other academic), Joint Work, Accepted

天疱瘡における抗CD20抗体療法

AMAGAI MASAYUKI

感染・炎症・免疫 44 ( 2 ) 186 - 188 2014.06

Research paper (scientific journal), Joint Work, Accepted

Dual role of interleukin-23 in epicutaneously-sensitized asthma in mice

Masaki, K. Suzuki, Y. Kagawa, S. Kodama, M. Kabata, H. Miyata, J. Tanaka, K. Fukunaga, K. Sayama, K. Oguma, T. Kimura, T. Amagai, M. Betsuyaku, T. Asano, K.

Allergol Int 63 ( Suppl 1 ) 13 - 22 2014.05

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Use of cyclosporin A for successful management of steroid-resistant Sweet’s syndrome patient with possible myelodysplastic syndrome

Yasuda, F. Fujio, Y. Kakuta, R. Funakoshi, T. Amagai, M. Ohyama, M.

J Dermatol 41 ( 5 ) 465-466 2014.05

Joint Work, Accepted

Access to Document (DOI)

The novel SLCO2A1 heterozygous missense mutation p.E427K and nonsense mutation p.R603* in a female patient with pachydermoperiostosis with an atypical phenotype

Niizeki, H. Shiohama, A. Sasaki, T. Seki, A. Kabashima, K. Otsuka, A. Takeshita, M. Hirakiyama, A. Okuyama, T. Tanese, K. Ishiko, A. Amagai, M. Kudoh, J.

Br J Dermatol 170 ( 5 ) 1187-1189 2014.05

Joint Work, Accepted

Access to Document (DOI)

棘融解による成長期脱毛を伴った尋常性天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 68 ( 6 ) 389 - 394 2014.05

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

アバタセプトによる乾癬様皮疹を呈した1例

AMAGAI MASAYUKI

臨床皮膚科 68 ( 6 ) 398 - 402 2014.05

Research paper (scientific journal), Joint Work, Accepted, ISSN 0021-4973

Japanese guidelines for the management of pemphigus

Committee for Guidelines for the Management of Pemphigus Disease Amagai, M. Tanikawa, A. Shimizu, T. Hashimoto, T. Ikeda, S. Kurosawa, M. Niizeki, H. Aoyama, Y. Iwatsuki, K. Kitajima, Y.

J Dermatol 41 ( 6 ) 471 - 486 2014.05

Research paper (scientific journal), Joint Work, Accepted

Access to Document (DOI)

Genetic polymorphisms in the IL22 gene are associated with psoriasis vulgaris in a Japanese population (vol 71, pg 148, 2013)

Saeki, H. Hirota, T. Nakagawa, H. Tsunemi, Y. Kato, T. Shibata, S. Sugaya, M. Sato, S. Doi, S. Miyatake, A. Ebe, K. Noguchi, E. Ebihara, T. Amagai, M. Esaki, H. Takeuchi, S. Furue, M. Nakamura, Y. Tamari, M.

J Dermatol Sci 74 ( 1 ) 95 - 95 2014.04

Joint Work, Accepted

Access to Document (DOI)

天疱瘡：抗デスモグレイン抗体

AMAGAI MASAYUKI

分子リウマチ治療 7 ( 2 ) 57 - 60 2014.04

Joint Work

免疫学発見物語　天疱瘡抗原の発見とその後の展開

AMAGAI MASAYUKI

JSI Newsletter 22 ( 2 ) 33 2014.04

Scoring systems for blistering diseases in practice: why bother, and which one should you use?

Murrell, D. F. Amagai, M. Werth, V. P.

JAMA Dermatol 150 ( 3 ) 245-247 2014.03

Joint Work, Accepted

Access to Document (DOI)

Genetic polymorphism in the TRAF3IP2 gene is associated with psoriasis vulgaris in a Japanese population

Hayashi, M. Hirota, T. Saeki, H. Nakagawa, H. Ishiuji, Y. Matsuzaki, H. Tsunemi, Y. Kato, T. Shibata, S. Sugaya, M. Sato, S. Tada, Y. Doi, S. Miyatake, A. Ebe, K. Noguchi, E. Ebihara, T. Amagai, M. Esaki, H. Takeuchi, S. Furue, M. Tamari, M.

J Dermatol Sci 73 ( 3 ) 264-265 2014.03

Joint Work, Accepted

Access to Document (DOI)

Unusually extensive disseminated herpes zoster with multiple ulcer formation in a methotrexate-treated rheumatoid arthritis patient

Kakuta, R. Okata, U. Funakoshi, T. Fujio, Y. Inoue, N. Takahashi, S. Amagai, M. Ohyama, M.

J Dermatol 41 ( 2 ) 181-182 2014.02

Joint Work, Accepted

Access to Document (DOI)

Rheumatoid neutrophilic dermatosis with tense blister formation: A case report and review of the literature

Fujio, Y. Funakoshi, T. Nakayama, K. Amagai, M. Ohyama, M.

Australas J Dermatol 55 ( 1 ) e12 - e14 2014.02

Joint Work, Accepted

皮膚科の現状と未来　多様性を持つ集団であれ

AMAGAI MASAYUKI

臨床皮膚科 68 ( 1 ) 10 - 11 2014.01

Single Work

経皮感作はなぜ重要か？〜皮膚バリア障害からみたアトピー疾患〜

AMAGAI MASAYUKI

皮膚アレルギーフロンティア 11 ( 3 ) 131 - 135 2013.12

Joint Work

A homozygous nonsense mutation in the gene for Tmem79, a component for the lamellar granule secretory system, produces spontaneous eczema in an experimental model of atopic dermatitis

Sasaki, T. Shiohama, A. Kubo, A. Kawasaki, H. Ishida-Yamamoto, A. Yamada, T. Hachiya, T. Shimizu, A. Okano, H. Kudoh, J. Amagai, M.

J Allergy Clin Immunol 132 ( 5 ) 1111-1120 2013.11

Joint Work, Accepted

天疱瘡と免疫グロブリン大量静注療法

AMAGAI MASAYUKI

医学のあゆみ 247 ( 6 ) 568 - 569 2013.11

Joint Work

Mutations in SERPINB7, encoding a member of the serine protease inhibitor superfamily, cause nagashima-type palmoplantar keratosis

Kubo, A. Shiohama, A. Sasaki, T. Nakabayashi, K. Kawasaki, H. Atsugi, T. Sato, S. Shimizu, A. Mikami, S. Tanizaki, H. Uchiyama, M. Maeda, T. Ito, T. Sakabe, J. Heike, T. Okuyama, T. Kosaki, R. Kosaki, K. Kudoh, J. Hata, K. Umezawa, A. Tokura, Y. Ishiko, A. Niizeki, H. Kabashima, K. Mitsuhashi, Y. Amagai, M.

Am J Hum Genet 93 ( 5 ) 945-956 2013.11

Joint Work, Accepted

Access to Document (DOI)

Immune response involving the bulge region in addition to telogen conversion contributes to hair loss in a case of atypical drug-induced hypersensitivity syndrome

Watanabe-Okada, E. Nagao, K. Takahashi, H. Amagai, M. Ohyama, M.

J Dermatol 40 ( 11 ) 928-929 2013.11

Joint Work, Accepted

Access to Document (DOI)

Anti-type VII collagen autoantibodies, detected by enzyme-linked immunosorbent assay, fluctuate in parallel with clinical severity in patients with epidermolysis bullosa acquisita

Ito, Y. Kasai, H. Yoshida, T. Saleh, M. A. Amagai, M. Yamagami, J.

J Dermatol 40 ( 11 ) 864-868 2013.11

Joint Work, Accepted

Access to Document (DOI)

自己免疫性水疱症(天疱瘡・類天疱瘡)

AMAGAI MASAYUKI

SRL宝函 34 ( 3 ) 12 - 19 2013.10

Single Work

感じるサイエンス

AMAGAI MASAYUKI

皮膚科の臨床 55 ( 10 ) 1211 - 1212 2013.10

Single Work

Th17 cells carrying TCR recognizing epidermal autoantigen induce psoriasis-like skin inflammation

Nishimoto, S. Kotani, H. Tsuruta, S. Shimizu, N. Ito, M. Shichita, T. Morita, R. Takahashi, H. Amagai, M. Yoshimura, A.

J Immunol 191 ( 6 ) 3065 - 3672 2013.09

Joint Work, Accepted

アトピー性皮膚炎の皮膚バリアからみた病態

AMAGAI MASAYUKI

臨床免疫・アレルギー科 60 ( 3 ) 276 - 281 2013.09

Joint Work

Improvement of mucocutaneous involvement of immunoglobulin light chain amyloidosis after chemotherapy and hematopoietic stem cell transplantation for multiple myeloma

Hosokawa, R. Ohyama, M. Murakami, K. Abe, D. Yokoyama, K. Okamoto, S. Amagai, M.

J Dermatol 40 ( 9 ) 768-769 2013.09

Joint Work, Accepted

Access to Document (DOI)

Genetic polymorphisms in the IL22 gene are associated with psoriasis vulgaris in a Japanese population

J Dermatol Sci 71 ( 2 ) 148-150 2013.08

Joint Work, Accepted

ドセタキセルが奏効した進行期乳房外Paget病の3例

AMAGAI MASAYUKI

臨床皮膚科 67 ( 9 ) 657 - 662 2013.08

Joint Work, Accepted

Functional tight junction barrier localizes in the second layer of the stratum granulosum of human epidermis

Yoshida, K. Yokouchi, M. Nagao, K. Ishii, K. Amagai, M. Kubo, A.

J Dermatol Sci 71 ( 2 ) 89-99 2013.08

Joint Work, Accepted

骨低形成、脂肪萎縮を伴った顔面に生じたBecker母斑症候群の1例

AMAGAI MASAYUKI

臨床皮膚科 67 ( 8 ) 607 - 610 2013.07

Joint Work

Collapse of the keratin filament network through the expression of mutant keratin 6c observed in a case of focal plantar keratoderma

Kubo, A. Oura, Y. Hirano, T. Aoyama, Y. Sato, S. Nakamura, K. Takae, Y. Amagai, M.

J Dermatol 40 ( 7 ) 553-557 2013.07

Joint Work, Accepted

Ectopic expression of epidermal antigens renders the lung a target organ in paraneoplastic pemphigus

Hata, T. Nishimoto, S. Nagao, K. Takahashi, H. Yoshida, K. Ohyama, M. Yamada, T. Asano, K. Amagai, M.

J Immunol 191 ( 1 ) 83-90 2013.07

Joint Work

Human Induced Pluripotent Stem Cell-Derived Ectodermal Precursor Cells Contribute to Hair Follicle Morphogenesis In Vivo

Veraitch, O. Kobayashi, T. Imaizumi, Y. Akamatsu, W. Sasaki, T. Yamanaka, S. Amagai, M. Okano, H. Ohyama, M.

J Invest Dermatol 133 ( 6 ) 1479-1488 2013.06

Joint Work, Accepted

水疱性病変による食道狭窄を示した後天性表皮水疱症の1例

AMAGAI MASAYUKI

Progress of Digestive Endoscopy 82 ( 1 ) 94 - 95 2013.06

Joint Work

天疱瘡と表皮特異的T細胞による皮膚炎

AMAGAI MASAYUKI

最新医学 68 ( 6月増刊号 ) 1409 - 1419 2013.06

Joint Work

Pemphigus Disease Area Indexによる治療効果の評価が有用であった若年発症落葉状天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 67 ( 7 ) 491 - 496 2013.06

Joint Work, Accepted

Cutaneous plasmacytosis: Report of 6 cases with or without systemic involvement

Honda, R. Cerroni, L. Tanikawa, A. Ebihara, T. Amagai, M. Ishiko, A.

J Am Acad Dermatol 68 ( 6 ) 978-985 2013.06

Joint Work, Accepted

Access to Document (DOI)

Autoimmune reactivity against precursor form of desmoglein 1 in healthy Tunisians in the area of endemic pemphigus foliaceus

Toumi, A. Saleh, M. A. Yamagami, J. Abida, O. Kallel, M. Masmoudi, A. Makni, S. Turki, H. Hachiya, T. Kuroda, K. Stanley, J. R. Masmoudi, H. Amagai, M.

J Dermatol Sci 70 ( 1 ) 19-25 2013.04

Joint Work, Accepted

The stratum corneum comprises three layers with distinct metal-ion barrier properties

Kubo, A. Ishizaki, I. Kawasaki, H. Nagao, K. Ohashi, Y. Amagai, M.

Sci Rep 3 1-6 2013.04

Joint Work, Accepted

Access to Document (DOI)

天疱瘡という「かたち」から見えてくる免疫学

AMAGAI MASAYUKI

JSI Newsletter 21 ( 2 ) 17 - 17 2013.04

Single Work

IgG deposition to non-hemidesmosomal lamina lucida and the early neutrophil involvement are the characteristic features in a case with anti-p200 pemphigoid

Shimizu, A. Funakoshi, T. Ishibashi, M. Yoshida, T. Koga, H. Hashimoto, T. Amagai, M. Ishiko, A.

Br J Dermatol 168 ( 3 ) 647 - 655 2013.03

Joint Work, Accepted

Access to Document (DOI)

Response to hydroxychloroquine in Japanese patients with lupus-related skin disease using the cutaneous lupus erythematosus disease area and severity index (CLASI)

Yokogawa, N. Tanikawa, A. Amagai, M. Kato, Y. Momose, Y. Arai, S. Eto, H. Ikeda, T. Furukawa, F.

Mod Rheumatol 23 ( 2 ) 318-322 2013.03

Research paper (scientific journal), Joint Work, Accepted

A pathophysiologic role for epidermal growth factor receptor in pemphigus acantholysis

Bektas, M. Jolly, P. S. Berkowitz, P. Amagai, M. Rubenstein, D. S.

J Biol Chem 288 ( 13 ) 9447-9456 2013.03

Accepted

Characterization of canine filaggrin: gene structure and protein expression in dog skin

Kanda, S. Sasaki, T. Shiohama, A. Nishifuji, K. Amagai, M. Iwasaki, T. Kudoh, J.

Vet Dermatol 24 ( 1 ) 25-31 2013.02

Joint Work, Accepted

Access to Document (DOI)

フィラグリン欠損による角層バリア障害とアトピー性皮膚炎

AMAGAI MASAYUKI

臨床免疫・アレルギー科 59 ( 2 ) 153 - 159 2013.02

Joint Work

自己免疫疾患、天疱瘡の病態解明と臨床応用

AMAGAI MASAYUKI

最新医学 68 ( 2 ) 248 - 301 2013.02

Single Work

Alopecia as a rare but distinct manifestation of pemphigus vulgaris

Veraitch, O. Ohyama, M. Yamagami, J. Amagai, M.

J Eur Acad Dermatol Venereol 27 ( 1 ) 86-91 2013.01

Accepted

Signaling dependent and independent mechanisms in pemphigus vulgaris blister formation

Saito, M. Stahley, S. N. Caughman, C. Y. Mao, X. Tucker, D. K. Payne, A. S. Amagai, M. Kowalczyk, A. P.

PLoS One 7 ( 12 ) e50696 - e50696 2012.12

Joint Work, Accepted

イヌ毛包細胞の遺伝子発現に対するロキシスロマイシンの与える影響の検討

AMAGAI MASAYUKI

皮膚の科学 11 ( 増刊19 ) 4 - 10 2012.12

Joint Work, Accepted

皮膚バリアの3次元構造（図説）

AMAGAI MASAYUKI

臨床のあゆみ 94 13 - 14 2012.12

Single Work

（病態・治療Q&A）皮膚バリアについてお教えください

AMAGAI MASAYUKI

臨床のあゆみ 94 7 - 8 2012.12

Single Work

Identification of mutations in the prostaglandin transporter gene SLCO2A1 and its phenotype-genotype correlation in Japanese patients with pachydermoperiostosis

Sasaki, T. Niizeki, H. Shimizu, A. Shiohama, A. Hirakiyama, A. Okuyama, T. Seki, A. Kabashima, K. Otsuka, A. Ishiko, A. Tanese, K. Miyakawa, S. I. Sakabe, J. I. Kuwahara, M. Amagai, M. Okano, H. Suematsu, M. Kudoh, J.

J Dermatol Sci 68 ( 1 ) 36 - 44 2012.10

Joint Work, Accepted

Mitochondrial dysfunction associated with increased oxidative stress and alpha-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue

Imaizumi, Y. Okada, Y. Akamatsu, W. Koike, M. Kuzumaki, N. Hayakawa, H. Nihira, T. Kobayashi, T. Ohyama, M. Sato, S. Takanashi, M. Funayama, M. Hirayama, A. Soga, T. Hishiki, T. Suematsu, M. Yagi, T. Ito, D. Kosakai, A. Hayashi, K. Shouji, M. Nakanishi, A. Suzuki, N. Mizuno, Y. Mizushima, N. Amagai, M. Uchiyama, Y. Mochizuki, H. Hattori, N. Okano, H.

Mol Brain 5 35 - 35 2012.10

Joint Work, Accepted

Genome-wide association study identifies eight new susceptibility loci for atopic dermatitis in the Japanese population

Hirota, T. Takahashi, A. Kubo, M. Tsunoda, T. Tomita, K. Sakashita, M. Yamada, T. Fujieda, S. Tanaka, S. Doi, S. Miyatake, A. Enomoto, T. Nishiyama, C. Nakano, N. Maeda, K. Okumura, K. Ogawa, H. Ikeda, S. Noguchi, E. Sakamoto, T. Hizawa, N. Ebe, K. Saeki, H. Sasaki, T. Ebihara, T. Amagai, M. Takeuchi, S. Furue, M. Nakamura, Y. Tamari, M.

Nat Genet 44 ( 11 ) 1222 - 1226 2012.10

Joint Work, Accepted

Pemphigus autoantibodies generated through somatic mutations target the desmoglein-3 cis-interface

Di Zenzo, G. Di Lullo, G. Corti, D. Calabresi, V. Sinistro, A. Vanzetta, F. Didona, B. Cianchini, G. Hertl, M. Eming, R. Amagai, M. Ohyama, B. Hashimoto, T. Sloostra, J. Sallusto, F. Zambruno, G. Lanzavecchia, A.

J Clin Invest 122 ( 10 ) 3781 - 3790 2012.10

Joint Work, Accepted

天疱瘡を通じた自己免疫疾患の発症機序解明への挑戦

AMAGAI MASAYUKI

医学のあゆみ 242 ( 10 ) 811 - 815 2012.09

Joint Work

Restoration of the intrinsic properties of human dermal papilla in vitro

Ohyama, M. Kobayashi, T. Sasaki, T. Shimizu, A. Amagai, M.

J Cell Sci 125 ( pt 17 ) 4114 - 4125 2012.09

Research paper (scientific journal), Joint Work, Accepted

乳癌の放射線治療部位に生じた限局性水疱性類天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 66 ( 10 ) 757 - 760 2012.09

Joint Work, Accepted

Brief Report: Requirement of TACE/ADAM17 for Hair Follicle Bugle Niche Establishment

Nagao, K. Kobayashi, T. Ohyama, M. Akiyama, H. Horiuchi, K. Amagai, M.

Stem Cells 30 ( 8 ) 1781-1785 2012.08

Research paper (scientific journal), Joint Work, Accepted

無治療で軽快したdiffuse neonatal hemangiomatosisの1例

AMAGAI MASAYUKI

臨床皮膚科 66 ( 8 ) 615-619 2012.07

Research paper (scientific journal), Joint Work, Accepted

Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin

Nagao, K. Kobayashi, T. Moro, K. Ohyama, M. Adachi, T. Kitashima, D. Y. Ueha, S. Horiuchi, K. Tanizaki, H. Kabashima, K. Kubo, A. Cho, Y. H. Clausen, B. E. Matsushima, K. Suematsu, M. Furtado, G. C. Lira, S. A. Farber, J. M. Udey, M. C. Amagai, M.

Nat Immunol 13 ( 8 ) 744-752 2012.06

Research paper (scientific journal), Joint Work, Accepted

Altered stratum corneum barrier and enhanced percutaneous immune responses in filaggrin-null mice

Kawasaki, H. Nagao, K. Kubo, A. Hata, T. Shimizu, A. Mizuno, H. Yamada, T. Amagai, M.

J Allergy Clin Immunol 129 ( 6 ) 1538-1546 2012.06

Research paper (scientific journal), Joint Work, Accepted

Mutations in the SASPase gene (ASPRV1) are not associated with atopic eczema or clinically dry skin

Sandilands, A. Brown, S. J. Goh, C. S. Pohler, E. Wilson, N. J. Campbell, L. E. Miyamoto, K. Kubo, A. Irvine, A. D. Thawer-Esmail, F. Munro, C. S. McLean, W. H. Kudoh, J. Amagai, M. Matsui, T.

J Invest Dermatol 132 ( 5 ) 1507-1510 2012.05

Research paper (scientific journal), Joint Work, Accepted

Autoimmunity against M(2) muscarinic acetylcholine receptor induces myocarditis and leads to a dilated cardiomyopathy-like phenotype

Yoshizawa, A. Nagai, S. Baba, Y. Yamada, T. Matsui, M. Tanaka, H. Miyoshi, S. I. Amagai, M. Yoshikawa, T. Fukuda, K. Ogawa, S. Koyasu, S.

Eur J Immunol 42 ( 5 ) 1152-1163 2012.05

Research paper (scientific journal), Joint Work, Accepted

Epitope spreading is rarely found in pemphigus vulgaris by large-scale longitudinal study using desmoglein 2-based swapped molecules

Ohyama, B. Nishifuji, K. Chan, P. T. Kawaguchi, A. Yamashita, T. Ishii, N. Hamada, T. Dainichi, T. Koga, H. Tsuruta, D. Amagai, M. Hashimoto, T.

J Invest Dermatol 132 ( 4 ) 1158-1168 2012.04

Research paper (scientific journal), Joint Work, Accepted

骨吸収を伴った小児口唇の線状強皮症の1例

AMAGAI MASAYUKI

臨床皮膚科 66 ( 4 ) 311-315 2012.04

Research paper (scientific journal), Joint Work, Accepted

天疱瘡　リツキシマブは有効か?

AMAGAI MASAYUKI

Derma. 190 91-93 2012.04

Research paper (scientific journal), Joint Work

Pathogenic anti-desmoglein 3 mAbs cloned from a paraneoplastic pemphigus patient by phage display

Saleh, M. A. Ishii, K. Yamagami, J. Shirakata, Y. Hashimoto, K. Amagai, M.

J Invest Dermatol 132 ( 4 ) 1141-1148 2012.04

Research paper (scientific journal), Joint Work, Accepted

Definitions and outcome measures for bullous pemphigoid: recommendations by an international panel of experts

Murrell, D. F. Daniel, B. S. Joly, P. Borradori, L. Amagai, M. Hashimoto, T. Caux, F. Marinovic, B. Sinha, A. A. Hertl, M. Bernard, P. Sirois, D. Cianchini, G. Fairley, J. A. Jonkman, M. F. Pandya, A. G. Rubenstein, D. Zillikens, D. Payne, A. S. Woodley, D. Zambruno, G. Aoki, V. Pincelli, C. Diaz, L. Hall, R. P. Meurer, M. Mascaro, J. M., Jr. Schmidt, E. Shimizu, H. Zone, J. Swerlick, R. Mimouni, D. Culton, D. Lipozencic, J. Bince, B. Grando, S. A. Bystryn, J. C. Werth, V. P.

J Am Acad Dermatol 66 ( 3 ) 479-485 2012.03

Research paper (scientific journal), Joint Work, Accepted

Desmoglein as a target in skin disease and beyond

Amagai, M. Stanley, J. R.

J Invest Dermatol 132 ( 3 ) 776-784 2012.03

Research paper (scientific journal), Joint Work

Epidermal barrier dysfunction and cutaneous sensitization in atopic diseases

Kubo, A. Nagao, K. Amagai, M.

J Clin Invest 122 ( 2 ) 440-447 2012.02

Research paper (scientific journal), Joint Work, Accepted

多発無菌性膿瘍・骨髄炎を伴った壊疽性膿皮症の1例

AMAGAI MASAYUKI

臨床皮膚科 66 ( 1 ) 49-52 2012.01

Research paper (scientific journal), Joint Work, Accepted

Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome

Ouchi, T. Kubo, A. Yokouchi, M. Adachi, T. Kobayashi, T. Kitashima, D. Y. Fujii, H. Clausen, B. E. Koyasu, S. Amagai, M. Nagao, K.

J Exp Med 208 ( 13 ) 2607-2613 2011.12

Research paper (scientific journal), Joint Work, Accepted

基礎研究から明らかとなった感作と発症の機序　皮膚バリア機能異常と抗原感作

AMAGAI MASAYUKI

アレルギー・免疫 19 ( 1 ) 32-39 2011.12

Research paper (scientific journal), Joint Work

環境と皮膚免疫フィラグリンと皮膚バリア機能

AMAGAI MASAYUKI

日本香粧品学会誌 35 ( 4 ) 318-319 2011.12

Research paper (scientific journal), Single Work

下顎に生じた常色結節の1例

AMAGAI MASAYUKI

日本皮膚病理組織学会会誌 27 ( 1 ) 25-28 2011.12

Research paper (scientific journal), Joint Work, Accepted

Variants of C-C Motif Chemokine 22 (CCL22) Are Associated with Susceptibility to Atopic Dermatitis: Case-Control Studies

Hirota, T. Saeki, H. Tomita, K. Tanaka, S. Ebe, K. Sakashita, M. Yamada, T. Fujieda, S. Miyatake, A. Doi, S. Enomoto, T. Hizawa, N. Sakamoto, T. Masuko, H. Sasaki, T. Ebihara, T. Amagai, M. Esaki, H. Takeuchi, S. Furue, M. Noguchi, E. Kamatani, N. Nakamura, Y. Kubo, M. Tamari, M.

PLoS One 6 ( 11 ) e26987 2011.11

Research paper (scientific journal), Joint Work, Accepted

Leser-Trelat症候群と内臓悪性腫瘍と関連の深い皮膚病変

AMAGAI MASAYUKI

外科 73 ( 12 ) 1278-1281 2011.11

Research paper (scientific journal), Joint Work, Accepted

Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of culture conditions

Kobayashi, T. Fujisawa, A. Amagai, M. Iwasaki, T. Ohyama, M.

Vet Dermatol 22 ( 5 ) 414-422 2011.10

Research paper (scientific journal), Joint Work, Accepted

そのほかの自己免疫疾患自己免疫性水疱症

AMAGAI MASAYUKI

臨床検査 55 ( 11 ) 1318-1326 2011.10

Research paper (scientific journal), Joint Work

Desmoglein 3-specific CD4+ T cells induce pemphigus vulgaris and interface dermatitis in mice

Takahashi, H. Kouno, M. Nagao, K. Wada, N. Hata, T. Nishimoto, S. Iwakura, Y. Yoshimura, A. Yamada, T. Kuwana, M. Fujii, H. Koyasu, S. Amagai, M.

J Clin Invest 121 ( 9 ) 3677-3688 2011.09

Research paper (scientific journal), Joint Work, Accepted

Pathogenic relevance of IgG and IgM antibodies against desmoglein 3 in blister formation in pemphigus vulgaris

Tsunoda, K. Ota, T. Saito, M. Hata, T. Shimizu, A. Ishiko, A. Yamada, T. Nakagawa, T. Kowalczyk, A. P. Amagai, M.

Am J Pathol 179 ( 2 ) 795-806 2011.08

Research paper (scientific journal), Joint Work, Accepted

アトピー性皮膚炎のゲノム解析の現況

AMAGAI MASAYUKI

アレルギー・免疫 18 ( 9 ) 1322-1329 2011.08

Research paper (scientific journal), Joint Work

ステロイドパルス・大量免疫グロブリン療法が奏功した高齢者重症尋常性天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 65 ( 8 ) 593-597 2011.07

Research paper (scientific journal), Joint Work, Accepted

Transgenic rescue of desmoglein 3 null mice with desmoglein 1 to develop a syngeneic mouse model for pemphigus vulgaris

Hata, T. Nishifuji, K. Shimoda, K. Sasaki, T. Yamada, T. Nishikawa, T. Koyasu, S. Amagai, M.

Journal of Dermatological Science 63 ( 1 ) 33-39 2011.07

Research paper (scientific journal), Joint Work, Accepted

Ichthyosis follicularis, alopecia, and photophobia syndrome: a case report and a pathological insight into pilosebaceous anomaly

Kamo, M. Ohyama, M. Kosaki, K. Amagai, M. Ebihara, T. Nakayama, J. Ishiko, A.

Am J Dermatopathol 33 ( 4 ) 403-406 2011.06

Research paper (scientific journal), Joint Work, Accepted

皮膚バリア機構とアトピー性皮膚炎

AMAGAI MASAYUKI

実験医学 29 ( 10 ) 1634-1640 2011.06

Research paper (scientific journal), Joint Work

血中自己抗体の検索を行ったDuhring疱疹状皮膚炎の1例

AMAGAI MASAYUKI

臨床皮膚科 65 ( 7 ) 487-491 2011.06

Research paper (scientific journal), Joint Work, Accepted

SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing

Matsui, T. Miyamoto, K. Kubo, A. Kawasaki, H. Ebihara, T. Hata, K. Tanahashi, S. Ichinose, S. Imoto, I. Inazawa, J. Kudoh, J. Amagai, M.

EMBO Mol Med 3 ( 6 ) 320-333 2011.06

Research paper (scientific journal), Joint Work, Accepted

Development of NC1 and NC2 domains of type VII collagen ELISA for the diagnosis and analysis of the time course of epidermolysis bullosa acquisita patients

Saleh, M. A. Ishii, K. Kim, Y. J. Murakami, A. Ishii, N. Hashimoto, T. Schmidt, E. Zillikens, D. Shirakata, Y. Hashimoto, K. Kitajima, Y. Amagai, M.

J Dermatol Sci 62 ( 3 ) 169-175 2011.06

Research paper (scientific journal), Joint Work, Accepted

Antigen-independent development of Foxp3+ regulatory T cells suppressing autoantibody production in experimental pemphigus vulgaris

Yokoyama, T. Matsuda, S. Takae, Y. Wada, N. Nishikawa, T. Amagai, M. Koyasu, S.

Int Immunol 23 ( 6 ) 365-373 2011.06

Research paper (scientific journal), Joint Work, Accepted

Loss-of-function mutations within the filaggrin gene and atopic dermatitis

Kawasaki, H. Kubo, A. Sasaki, T. Amagai, M.

Curr Probl Dermatol 41 35-46 2011.05

Research paper (scientific journal), Joint Work, Accepted

アレルギー性皮膚疾患とバリア機能異常

AMAGAI MASAYUKI

小児科 52 ( 6 ) 833-841 2011.05

Research paper (scientific journal), Joint Work

Exercise-induced vasculitisの1例

AMAGAI MASAYUKI

臨床皮膚科 65 ( 6 ) 396-399 2011.05

Research paper (scientific journal), Joint Work, Accepted

Prevalence of dermatological disorders in Japan: A nationwide, cross-sectional, seasonal, multicenter, hospital-based study

Furue, M. Yamazaki, S. Jimbow, K. Tsuchida, T. Amagai, M. Tanaka, T. Matsunaga, K. Muto, M. Morita, E. Akiyama, M. Soma, Y. Terui, T. Manabe, M.

J Dermatol 38 ( 4 ) 310-320 2011.04

Research paper (scientific journal), Joint Work, Accepted

Langerhans細胞組織球症の皮膚症状を伴ったErdheim-Chester病の1例

AMAGAI MASAYUKI

臨床皮膚科 65 ( 4 ) 338-342 2011.04

Research paper (scientific journal), Joint Work, Accepted

Desmosome disassembly in response to pemphigus vulgaris IgG occurs in distinct phases and can be reversed by expression of exogenous dsg3

Jennings, J. M. Tucker, D. K. Kottke, M. D. Saito, M. Delva, E. Hanakawa, Y. Amagai, M. Kowalczyk, A. P.

J Invest Dermatol 131 ( 3 ) 706-718 2011.03

Research paper (scientific journal), Joint Work, Accepted

自己免疫性水疱症ー今日の進歩　はじめに

AMAGAI MASAYUKI

医学のあゆみ 236 ( 11 ) 1013 2011.03

Research paper (scientific journal), Single Work

自己免疫性水疱症：病態と診断

山上淳　天谷雅行

日本皮膚科学会雑誌 121 ( 2 ) 135-143 2011.02

Research paper (scientific journal), Joint Work

皮膚科セミナリウム(第70回)　水疱症　自己免疫性水疱症　病態と診断

AMAGAI MASAYUKI

日本皮膚科学会雑誌 121 ( 2 ) 135-143 2011.02

Research paper (scientific journal), Joint Work

Aire-dependent thymic expression of desmoglein 3, the autoantigen in pemphigus vulgaris, and its role in T-cell tolerance

Wada, N. Nishifuji, K. Yamada, T. Kudoh, J. Shimizu, N. Matsumoto, M. Peltonen, L. Nagafuchi, S. Amagai, M.

J Invest Dermatol 131 ( 2 ) 410-417 2011.02

Research paper (scientific journal), Joint Work, Accepted

Generation of human melanocytes from induced pluripotent stem cells

Ohta, S. Imaizumi, Y. Okada, Y. Akamatsu, W. Kuwahara, R. Ohyama, M. Amagai, M. Matsuzaki, Y. Yamanaka, S. Okano, H. Kawakami, Y.

PLoS One 6 ( 1 ) 1-10 2011.01

Research paper (scientific journal), Joint Work, Accepted

皮膚バリア機構の新しい理解ー体表面における防御と索敵のメカニズム

AMAGAI MASAYUKI

HUMAN SCIENCE 22 ( 1 ) 14-18 2011.01

Research paper (scientific journal), Joint Work

難治性皮膚疾患治療の現状と将来

AMAGAI MASAYUKI

HUMAN SCIENCE 22 ( 1 ) 4-13 2011.01

Research paper (scientific journal), Joint Work

Overview on autoimmunity and autoinflammation

Amagai, M. Matsushima, K.

Inflammation and Regeneration 31 ( 1 ) 50-51 2011.01

Research paper (scientific journal), Joint Work, Accepted

Autoimmunity to desmocollin 3 in pemphigus vulgaris

Mao, X. Nagler, A. R. Farber, S. A. Choi, E. J. Jackson, L. H. Leiferman, K. M. Ishii, N. Hashimoto, T. Amagai, M. Zone, J. J. Payne, A. S.

Am J Pathol 177 ( 6 ) 2724-2730 2010.12

Research paper (scientific journal), Joint Work, Accepted

ランゲルハンス細胞による抗原の補捉機序

AMAGAI MASAYUKI

臨床免疫・アレルギー科 54 ( 6 ) 701-709 2010.12

Research paper (scientific journal), Joint Work

天疱瘡におけるDsg3分子と制御

AMAGAI MASAYUKI

Medical Science Digest 36 ( 12 ) 1085-1087 2010.11

Research paper (scientific journal), Joint Work

ELISA法

AMAGAI MASAYUKI

皮膚病診療 32 ( Suppl ) 115-117 2010.11

Research paper (scientific journal), Joint Work

ランゲルハンス細胞による外来抗原捕捉機構

久保亮治　天谷雅行

感染・炎症・免疫 40 ( 3 ) 267-270 2010.10

Research paper (scientific journal), Joint Work

巨大な多発性痛風結節の1例

本多皓　馬場あゆみ　高江雄二郎　天谷雅行

Visual Dermatology 9 ( 10 ) 1032-1033 2010.09

Research paper (scientific journal), Joint Work, Accepted

巨大な多発性痛風結節の1例

AMAGAI MASAYUKI

Visual Dermatology 9 ( 10 ) 1032-1033 2010.09

Research paper (scientific journal), Joint Work, Accepted

Removal of amino-terminal extracellular domains of desmoglein 1 by staphylococcal exfoliative toxin is sufficient to initiate epidermal blister formation

Nishifuji, K. Shimizu, A. Ishiko, A. Iwasaki, T. Amagai, M.

J Dermatol Sci 59 ( 3 ) 184-191 2010.09

Research paper (scientific journal), Joint Work, Accepted

IgA pemphigus reacting exclusively to desmoglein 3

Tajima, M. Mitsuhashi, Y. Irisawa, R. Amagai, M. Hashimoto, T. Tsuboi, R.

Eur J Dermatol 20 ( 5 ) 626-629 2010.09

Research paper (scientific journal), Joint Work, Accepted

Canine Follicle Stem Cell Candidates Reside in the Bulge and Share Characteristic Features with Human Bulge Cells

Kobayashi, T. Iwasaki, T. Amagai, M. Ohyama, M.

J Invest Dermatol 130 ( 8 ) 1988-1995 2010.08

Research paper (scientific journal), Joint Work, Accepted

Simple PCR-based DNA microarray system to identify human pathogenic fungi in skin

Sato, T. Takayanagi, A. Nagao, K. Tomatsu, N. Fukui, T. Kawaguchi, M. Kudoh, J. Amagai, M. Yamamoto, N. Shimizu, N.

J Clin Microbiol 48 ( 7 ) 2357-2364 2010.07

Research paper (scientific journal), Joint Work, Accepted

カルボシステイン（ムコイダン®）による多発性固定薬疹の1例

AMAGAI MASAYUKI

臨床皮膚科 64 ( 8 ) 550-553 2010.07

Research paper (scientific journal), Joint Work, Accepted

Immune response towards the amino-terminus of desmoglein 1 prevails across different activity stages in nonendemic pemphigus foliaceus

Chan, P. T. Ohyama, B. Nishifuji, K. Yoshida, K. Ishii, K. Hashimoto, T. Amagai, M.

Br J Dermatol 162 ( 6 ) 1242-1250 2010.06

Research paper (scientific journal), Joint Work, Accepted

抗デスモグレイン1および3抗体、抗BP180(NC16a）抗体、抗BP230抗体

AMAGAI MASAYUKI

日本臨床 68 ( 増6 ) 614-617 2010.06

Research paper (scientific journal), Single Work, Accepted

2回の生検により診断確定に至ったDuhring疱疹状皮膚炎の1例

AMAGAI MASAYUKI

臨床皮膚科 64 ( 7 ) 464-467 2010.06

Research paper (scientific journal), Joint Work, Accepted

抗デスモグレイン抗体と天疱瘡

AMAGAI MASAYUKI

Mebio 27 ( 6 ) 102-109 2010.06

Research paper (scientific journal), Joint Work, Accepted

天疱瘡診療ガイドライン

AMAGAI MASAYUKI

日本皮膚科学会雑誌 120 ( 7 ) 1443-1460 2010.06

Research paper (scientific journal), Joint Work, Accepted

Experimental evaluation of ebastine, a second-generation anti-histamine, as a supportive medication for alopecia areata

Ohyama, M. Shimizu, A. Tanaka, K. Amagai, M.

J Dermatol Sci 58 ( 2 ) 154-157 2010.05

Research paper (scientific journal), Joint Work

Flaky Tail Mouse Denotes Human Atopic Dermatitis in the Steady State and by Topical Application with Dermatophagoides pteronyssinus Extract

Moniaga, C. S. Egawa, G. Kawasaki, H. Hara-Chikuma, M. Honda, T. Tanizaki, H. Nakajima, S. Otsuka, A. Matsuoka, H. Kubo, A. Sakabe, J. I. Tokura, Y. Miyachi, Y. Amagai, M. Kabashima, K.

Am J Pathol 176 ( 5 ) 2385-2393 2010.05

Research paper (scientific journal), Joint Work, Accepted

Experimental evaluation of ebastine, a second-generation anti-histamine, as a supportive medication for alopecia areata

Ohyama, M. Shimizu, A. Tanaka, K. Amagai, M.

J Dermatol Sci 58 ( 2 ) 154-157 2010.05

Research paper (scientific journal), Joint Work, Accepted

皮膚バリア研究の新展開

AMAGAI MASAYUKI

FRAGRANCE JOURNAL 38 ( 5 ) 14-18 2010.05

Research paper (scientific journal), Joint Work, Accepted

診断の指針　治療の指針　自己免疫水疱症の検査法

AMAGAI MASAYUKI

綜合臨床 59 ( 5 ) 1289-1290 2010.05

Research paper (scientific journal), Joint Work, Accepted

Autoimmune and infectious skin diseases that target desmogleins

AMAGAI MASAYUKI

Proc Jpn Acad Ser 86 ( 5 ) 524-537 2010.05

Research paper (scientific journal), Single Work

顔面神経麻痺を伴った多菌型Hansen病の1例

AMAGAI MASAYUKI

臨床皮膚科 64 ( 4 ) 337-341 2010.04

Research paper (scientific journal), Joint Work, Accepted

自己免疫性水疱症の診断

AMAGAI MASAYUKI

日本医事新報 ( 4485 ) 65-68 2010.04

Research paper (scientific journal), Joint Work, Accepted

難治疾患　未解明の研究課題と治療への挑戦　天疱瘡自己抗体の産生におけるT細胞の役割

AMAGAI MASAYUKI

実験医学 28 ( 6 ) 947-952 2010.04

Research paper (scientific journal), Joint Work, Accepted

Immune response towards the amino-terminus of desmoglein 1 prevails across different activity stages in nonendemic pemphigus foliaceus

Chan, P. T. Ohyama, B. Nishifuji, K. Yoshida, K. Ishii, K. Hashimoto, T. Amagai, M.

Br J Dermatol 37 ( 3 ) 205-213 2010.03

Research paper (scientific journal), Joint Work, Accepted

乾癬の病態と分子標的

AMAGAI MASAYUKI

治療学 44 ( 2 ) 162-166 2010.02

Research paper (scientific journal), Joint Work, Accepted

伝染性膿痂疹

AMAGAI MASAYUKI

小児内科 42 ( 1 ) 129-132 2010.01

Research paper (scientific journal), Joint Work, Accepted

Changes to JSID--from a closed to an open society

Amagai, M.

J Dermatol Sci 57 ( 1 ) 1 2010.01

Research paper (scientific journal), Single Work, Accepted

Blepharochalasisの1例

AMAGAI MASAYUKI

臨床皮膚科 64 ( 1 ) 33-36 2010.01

Research paper (scientific journal), Joint Work, Accepted

External antigen uptake by Langerhans cells with reorganization of epidermal tight junction barriers

Kubo, A. Nagao, K. Yokouchi, M. Sasaki, H. Amagai, M.

J Exp Med 206 ( 13 ) 2937-2946 2009.12

Research paper (scientific journal), Joint Work, Accepted

多形紅斑様皮疹が先行したChurg-Strauss症候群の1例

AMAGAI MASAYUKI

臨床皮膚科 63 ( 12 ) 900-904 2009.11

Research paper (scientific journal), Joint Work, Accepted

天疱瘡　ー重症度判定基準を中心に

AMAGAI MASAYUKI

医学のあゆみ 230 ( 11 ) 997-1000 2009.11

Research paper (scientific journal), Joint Work, Accepted

疥癬に対するイベルメクチン内服療法の臨床的検討

AMAGAI MASAYUKI

日本皮膚科学会雑誌 119 ( 9 ) 1845-1850 2009.11

Research paper (scientific journal), Joint Work, Accepted

抗デスモグレイン1抗体陽性IgG/IgA天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 63 ( 12 ) 927-931 2009.11

Research paper (scientific journal), Joint Work, Accepted

The molecular logic of pemphigus and impetigo: the desmoglein story

Amagai, M.

Vet Dermatol 20 ( 5-6 ) 308-312 2009.10

Research paper (scientific journal), Single Work, Accepted

Canine hair-follicle keratinocytes enriched with bulge cells have the highly proliferative characteristic of stem cells

Kobayashi, T. Shimizu, A. Nishifuji, K. Amagai, M. Iwasaki, T. Ohyama, M.

Vet Dermatol 20 ( 5-6 ) 338-346 2009.10

Research paper (scientific journal), Joint Work, Accepted

Development of an enzyme-linked immunosorbent assay for detection of circulating IgG autoantibodies against canine desmoglein 3 in dogs with pemphigus

Nishifuji, K. Tamura, K. Konno, H. Olivry, T. Amagai, M. Iwasaki, T.

Vet Dermatol 20 ( 5-6 ) 331-337 2009.10

Research paper (scientific journal), Joint Work, Accepted

Pathogenic Epitopes of Autoantibodies in Pemphigus Reside in the Amino-Terminal Adhesive Region of Desmogleins Which Are Unmasked by Proteolytic Processing of Prosequence

Yokouchi, M. Saleh, M. A. Kuroda, K. Hachiya, T. Stanley, J. R. Amagai, M. Ishii, K.

J Invest Dermatol 129 ( 9 ) 2156-2166 2009.09

Research paper (scientific journal), Joint Work, Accepted

本邦における皮膚科受診患者の多施設横断四季別全国調査

AMAGAI MASAYUKI

日本皮膚科学会雑誌 119 ( 9 ) 1795-1809 2009.08

Research paper (scientific journal), Joint Work, Accepted

Subcellular localization of desmosomal components is different between desmoglein3 knockout mice and pemphigus vulgaris model mice

Saito, H. Shimizu, A. Tsunoda, K. Amagai, M. Ishiko, A.

J Dermatol Sci 55 ( 2 ) 108-115 2009.08

Research paper (scientific journal), Joint Work, Accepted

Intractable ulcer caused by Mycobacterium shinshuense: successful identification of mycobacterium strain by 16S ribosomal RNA 3'-end sequencing

Funakoshi, T. Kazumi, Y. Okada, R. Nishimoto, K. Saito, M. Amagai, M. Shimura, H. Ohyama, M.

Clin Exp Dermatol 34 ( 8 ) e712-715 2009.08

Research paper (scientific journal), Joint Work, Accepted

天疱瘡モデルマウスにおける病的抗体産生を制御するDsg3反応性T細胞の役割

AMAGAI MASAYUKI

医学のあゆみ 230 ( 9 ) 686-691 2009.08

Research paper (scientific journal), Joint Work, Accepted

IVIG

AMAGAI MASAYUKI

皮膚アレルギーフロンティア 7 ( 2 ) 58 2009.07

Research paper (scientific journal), Single Work, Accepted

特別講演会から　皮膚を科学する　ー水疱症から、とびひ、アトピー性皮膚炎へー

AMAGAI MASAYUKI

なごみ（慶應義塾大学薬学部KP会誌） 60 14-21 2009.07

Research paper (scientific journal), Single Work

A homozygous frameshift mutation in the mouse Flg gene facilitates enhanced percutaneous allergen priming

Fallon, P. G. Sasaki, T. Sandilands, A. Campbell, L. E. Saunders, S. P. Mangan, N. E. Callanan, J. J. Kawasaki, H. Shiohama, A. Kubo, A. Sundberg, J. P. Presland, R. B. Fleckman, P. Shimizu, N. Kudoh, J. Irvine, A. D. Amagai, M. McLean, W. H.

Nat Genet 41 ( 5 ) 602-608 2009.05

Research paper (scientific journal), Joint Work, Accepted

嚢腫型深在性白癬　Castleman病患者の臀部に生じた例

AMAGAI MASAYUKI

皮膚病診療 31 ( 4 ) 423-426 2009.04

Research paper (scientific journal), Joint Work, Accepted

Desmosome splitting is a primary ultrastructural change in the acantholysis of pemphigus

Wang, W. Amagai, M. Ishiko, A.

J Dermatol Sci 54 ( 1 ) 59-61 2009.04

Research paper (scientific journal), Joint Work, Accepted

A randomized double-blind trial of intravenous immunoglobulin for pemphigus

Amagai, M. Ikeda, S. Shimizu, H. Iizuka, H. Hanada, K. Aiba, S. Kaneko, F. Izaki, S. Tamaki, K. Ikezawa, Z. Takigawa, M. Seishima, M. Tanaka, T. Miyachi, Y. Katayama, I. Horiguchi, Y. Miyagawa, S. Furukawa, F. Iwatsuki, K. Hide, M. Tokura, Y. Furue, M. Hashimoto, T. Ihn, H. Fujiwara, S. Nishikawa, T. Ogawa, H. Kitajima, Y. Hashimoto, K.

J Am Acad Dermatol 60 ( 4 ) 595-603 2009.04

Research paper (scientific journal), Joint Work, Accepted

Pemphigus mouse model as a tool to evaluate various immunosuppressive therapies

Takae, Y. Nishikawa, T. Amagai, M.

Exp Dermatol 18 ( 3 ) 256-260 2009.03

Research paper (scientific journal), Joint Work, Accepted

IBDに合併する皮膚症状とその対策

AMAGAI MASAYUKI

IBD Research 3 ( 1 ) 37-41 2009.03

Research paper (scientific journal), Joint Work

A single helper T cell clone is sufficient to commit polyclonal naive B cells to produce pathogenic IgG in experimental pemphigus vulgaris

Takahashi, H. Kuwana, M. Amagai, M.

J Immunol 182 ( 3 ) 1740-1745 2009.02

Research paper (scientific journal), Joint Work

Inflammatory plaque with peripheral nodules: a new specific finding of cutaneous polyarteritis nodosa

Chan, P. T. Ishiko, A. Wada, N. Yamamoto, N. Amagai, M.

J Am Acad Dermatol 60 ( 2 ) 320-325 2009.02

Research paper (scientific journal), Joint Work, Accepted

Primary tuberculosis of the penis after intravesical bacillus Calmette-Guerin instillation therapy

Yoshida, R. Kawasaki, H. Miyajima, A. Amagai, M. Ohyama, M.

J Eur Acad Dermatol Venereol 23 ( 1 ) 77-78 2009.01

Research paper (scientific journal), Joint Work

Sentan: a novel specific component of the apical structure of vertebrate motile cilia

Kubo, A. Yuba-Kubo, A. Tsukita, S. Tsukita, S. Amagai, M.

Mol Biol Cell 19 ( 12 ) 5338-5346 2008.12

Research paper (scientific journal), Joint Work, Accepted

後頭部に生じた結節

AMAGAI MASAYUKI

日本皮膚病理組織学会会誌 24 ( 1 ) 6-9 2008.12

Research paper (scientific journal), Joint Work, Accepted

気道アレルギー発症への経皮感作の影響

AMAGAI MASAYUKI

Topics in Atopy 7 ( 4 ) 37-42 2008.12

Research paper (scientific journal), Joint Work

Genetic characterization of human Dsg3-specific B cells isolated by flow cytometry from the peripheral blood of patients with pemphigus vulgaris

Yamagami, J. Takahashi, H. Ota, T. Amagai, M.

J Dermatol Sci 52 ( 2 ) 98-107 2008.11

Research paper (scientific journal), Joint Work, Accepted

Candida albicans infection delays duodenal ulcer healing in cysteamine-induced duodenal ulcers in rats

Jin, L. Yoshida, M. Nakamura, T. Ishikawa, H. Wakabayashi, G. Tanabe, M. Kawachi, S. Shinoda, M. Saikawa, Y. Wada, N. Kameyama, K. Kumai, K. Kubota, T. Sano, K. Nagao, K. Amagai, M. Kitagawa, Y. Kitajima, M.

Dig Dis Sci 53 ( 11 ) 2878-2885 2008.11

Research paper (scientific journal), Joint Work, Accepted

Cutaneous pemphigus vulgaris with skin features similar to the classic mucocutaneous type: a case report and review of the literature

Shinkuma, S. Nishie, W. Shibaki, A. Sawamura, D. Ito, K. Tsuji-Abe, Y. Natsuga, K. Chan, P. T. Amagai, M. Shimizu, H.

Clin Exp Dermatol 33 ( 6 ) 724-728 2008.11

Research paper (scientific journal), Joint Work, Accepted

Autoreactive B-cell elimination by pathogenic IgG specific for the same antigen: implications for peripheral tolerance

Ota, T. Aoki-Ota, M. Tsunoda, K. Nishikawa, T. Koyasu, S. Amagai, M.

Int Immunol 20 ( 10 ) 1351-1360 2008.10

Research paper (scientific journal), Joint Work, Accepted

水膨れが生じる皮膚疾患

AMAGAI MASAYUKI

内科 102 ( 4 ) 781-785 2008.10

Research paper (scientific journal), Joint Work

デスモグレインと天疱瘡

AMAGAI MASAYUKI

炎症と免疫 16 ( 6 ) 717-719 2008.10

Research paper (scientific journal), Joint Work

G-protein-coupled receptor GPR49 is up-regulated in basal cell carcinoma and promotes cell proliferation and tumor formation

Tanese, K. Fukuma, M. Yamada, T. Mori, T. Yoshikawa, T. Watanabe, W. Ishiko, A. Amagai, M. Nishikawa, T. Sakamoto, M.

Am J Pathol 173 ( 3 ) 835-843 2008.09

Research paper (scientific journal), Joint Work, Accepted

Post-IID 2008 International Meeting on autoimmune bullous diseases

Amagai, M. Hashimoto, T. Kitajima, Y.

J Invest Dermatol 128 ( 9 ) 2135-2137 2008.09

Research paper (scientific journal), Joint Work, Accepted

Anti-desmoglein IgG autoantibodies in patients with pemphigus in remission

Kwon, E. J. Yamagami, J. Nishikawa, T. Amagai, M.

J Eur Acad Dermatol Venereol 22 ( 9 ) 1070-1075 2008.09

Research paper (scientific journal), Joint Work, Accepted

Sequence analysis of filaggrin gene by novel shotgun method in Japanese atopic dermatitis

Sasaki, T. Kudoh, J. Ebihara, T. Shiohama, A. Asakawa, S. Shimizu, A. Takayanagi, A. Dekio, I. Sadahira, C. Amagai, M. Shimizu, N.

J Dermatol Sci 51 ( 2 ) 113-120 2008.08

Research paper (scientific journal), Joint Work, Accepted

Novel system evaluating in vivo pathogenicity of desmoglein 3-reactive T cell clones using murine pemphigus vulgaris

Takahashi, H. Amagai, M. Nishikawa, T. Fujii, Y. Kawakami, Y. Kuwana, M.

J Immunol 181 ( 2 ) 1526-1535 2008.07

Research paper (scientific journal), Joint Work, Accepted

Pemphigus mouse model as a tool to evaluate various immunosuppressive therapies

Takae, Y. Nishikawa, T. Amagai, M.

Exp Dermatol 18 252-260 2008.07

Research paper (scientific journal), Joint Work, Accepted

Consensus statement on definitions of disease, end points, and therapeutic response for pemphigus

Murrell, D. F. Dick, S. Ahmed, A. R. Amagai, M. Barnadas, M. A. Borradori, L. Bystryn, J. C. Cianchini, G. Diaz, L. Fivenson, D. Hall, R. Harman, K. E. Hashimoto, T. Hertl, M. Hunzelmann, N. Iranzo, P. Joly, P. Jonkman, M. F. Kitajima, Y. Korman, N. J. Martin, L. K. Mimouni, D. Pandya, A. G. Payne, A. S. Rubenstein, D. Shimizu, H. Sinha, A. A. Sirois, D. Zillikens, D. Werth, V. P.

J Am Acad Dermatol 58 ( 6 ) 1043-1046 2008.06

Research paper (scientific journal), Joint Work, Accepted

詳細な自己抗体解析による天疱瘡の病態解明

AMAGAI MASAYUKI

実験医学 26 ( 8 ) 1302-1307 2008.05

Research paper (scientific journal), Joint Work

座談会　Clinical Immunologyの展望

AMAGAI MASAYUKI

Frontiers in Rheumatology & Clinical Immunology 2 ( 2 ) 69-81 2008.05

Single Work

Evaluating efficacy of plasmapheresis for patients with pemphigus using desmoglein enzyme-linked immunosorbent assay

Nagasaka, T. Fujii, Y. Ishida, A. Handa, M. Tanikawa, A. Amagai, M. Nishikawa, T.

Br J Dermatol 158 ( 4 ) 685-690 2008.04

Research paper (scientific journal), Joint Work, Accepted

バリア機能と皮膚疾患

AMAGAI MASAYUKI

交詢雑誌 515 54-72 2008.03

Research paper (scientific journal), Single Work

Rapid growth of malignant melanoma in pregnancy

Sato, T. Ishiko, A. Saito, M. Tanaka, M. Ishimoto, H. Amagai, M.

J Dtsch Dermatol Ges 6 ( 2 ) 126-129 2008.02

Research paper (scientific journal), Joint Work, Accepted

Staphylococcal exfoliative toxins: "Molecular scissors" of bacteria that attack the cutaneous defense barrier in mammals

Nishifuji, K. Sugai, M. Amagai, M.

J Dermatol Sci 49 ( 1 ) 21-31 2008.01

Research paper (scientific journal), Joint Work, Accepted

Characterization of skin microbiota in patients with atopic dermatitis and in normal subjects using 16S rRNA gene-based comprehensive analysis

Dekio, I. Sakamoto, M. Hayashi, H. Amagai, M. Suematsu, M. Benno, Y.

J Med Microbiol 56 ( 12 ) 1675-1683 2007.12

Research paper (scientific journal), Joint Work, Accepted

自己免疫　最近の進歩

AMAGAI MASAYUKI

日本皮膚科学会雑誌 117 ( 13 ) 2093-2094 2007.12

Research paper (scientific journal), Single Work, Accepted

皮膚免疫と天疱瘡

AMAGAI MASAYUKI

実験医学 25 ( 20（増） ) 193-198 2007.12

Research paper (scientific journal), Joint Work, Accepted

多房性ドーム状隆起を示した症例

AMAGAI MASAYUKI

皮膚病診療 29 ( 11 ) 1299-1302 2007.11

Research paper (scientific journal), Joint Work

Development of tissue-targeting hemagglutinating virus of Japan envelope vector for successful delivery of therapeutic gene to mouse skin

Kawachi, M. Tamai, K. Saga, K. Yamazaki, T. Fujita, H. Shimbo, T. Kikuchi, Y. Nimura, K. Nishifuji, K. Amagai, M. Uitto, J. Kaneda, Y.

Hum Gene Ther 18 ( 10 ) 881-894 2007.10

Research paper (scientific journal), Joint Work, Accepted

Mucinous nevusの1例

AMAGAI MASAYUKI

臨床皮膚科 61 ( 11 ) 869-872 2007.10

Research paper (scientific journal), Joint Work, Accepted

No activation of urokinase plasminogen activator by anti-desmoglein 3 monoclonal IgG antibodies in cultured human keratinocytes

Yamamoto, Y. Aoyama, Y. Shu, E. Tsunoda, K. Amagai, M. Kitajima, Y.

J Dermatol Sci 47 ( 2 ) 119-125 2007.08

Research paper (scientific journal), Joint Work, Accepted

Abnormal keratin expression in circumscribed palmar hypokeratosis

Ishiko, A. Dekio, I. Fujimoto, A. Kameyama, K. Sakamoto, M. Benno, Y. Amagai, M. Nishikawa, T.

J Am Acad Dermatol 57 ( 2 ) 285-291 2007.08

Research paper (scientific journal), Joint Work, Accepted

Canine hyperplastic intraepidermal pustular and suprabasal acantholytic dermatosis with features of human pemphigus vegetans

Heimann, M. Beco, L. Petein, M. Nishifuji, K. Amagai, M. Olivry, D. T.

Vet Pathol 44 ( 4 ) 550-555 2007.07

Research paper (scientific journal), Joint Work, Accepted

Anti-desmoglein 3 (Dsg3) monoclonal antibodies deplete desmosomes of Dsg3 and differ in their Dsg3-depleting activities related to pathogenicity

Yamamoto, Y. Aoyama, Y. Shu, E. Tsunoda, K. Amagai, M. Kitajima, Y.

J Biol Chem 282 ( 24 ) 17866-17876 2007.06

Research paper (scientific journal), Joint Work, Accepted

ブドウ球菌感染における皮膚病変とその機序—天疱瘡との類似点—

AMAGAI MASAYUKI

化学療法の領域 23 ( 7 ) 1061-1066 2007.06

Research paper (scientific journal), Single Work

天疱瘡自己抗体のあらたな病原性評価法の開発—In vitro dissociation assay: Development of novel aasay for pathogenic activity of anti-desmoglein 3 autoantibody

AMAGAI MASAYUKI

医学のあゆみ 221 ( 11 ) 921-925 2007.06

Research paper (scientific journal), Joint Work

IgG autoantibodies directed against desmoglein 3 cause dissociation of keratinocytes in canine pemphigus vulgaris and paraneoplastic pemphigus

Nishifuji, K. Olivry, T. Ishii, K. Iwasaki, T. Amagai, M.

Vet Immunol Immunopathol 117 ( 3-4 ) 209-221 2007.06

Research paper (scientific journal), Joint Work, Accepted

Imaging and force spectroscopy on desmoglein 1 using atomic force microscopy reveal multivalent Ca(2+)-dependent, low-affinity trans-interaction

Waschke, J. Menendez-Castro, C. Bruggeman, P. Koob, R. Amagai, M. Gruber, H. J. Drenckhahn, D. Baumgartner, W.

J Membr Biol 216 ( 2-3 ) 83-92 2007.04

Research paper (scientific journal), Joint Work, Accepted

自己免疫性皮膚疾患　基礎から臨床へ

AMAGAI MASAYUKI

感染・炎症・免疫 37 ( 1 ) 18-27 2007.03

Research paper (scientific journal), Single Work

T helper type 2-biased natural killer cell phenotype in patients with pemphigus vulgaris

Takahashi, H. Amagai, M. Tanikawa, A. Suzuki, S. Ikeda, Y. Nishikawa, T. Kawakami, Y. Kuwana, M.

J Invest Dermatol 127 ( 2 ) 324-330 2007.02

Research paper (scientific journal), Joint Work, Accepted

抗癌剤による薬疹

AMAGAI MASAYUKI

Visual Dermatology 6 ( 2 ) 156-157 2007.02

Research paper (scientific journal), Joint Work

Gene ontology analysis of human hair follicle bulge molecular signature

Ohyama, M. Vogel, J. C. Amagai, M.

J Dermatol Sci 45 ( 2 ) 147-150 2007.02

Research paper (scientific journal), Joint Work, Accepted

ヒトの天疱瘡における水疱形成メカニズム

AMAGAI MASAYUKI

Visual Veterinary Dermatology 3 ( 1 ) 7-12 2007.01

Research paper (scientific journal), Joint Work

Minocycline-induced cutaneous hyperpigmentation

AMAGAI MASAYUKI

皮膚病診療 29 ( 1 ) 47-50 2007.01

Research paper (scientific journal), Joint Work, Accepted

疥癬診療ガイドライン(第2版)

AMAGAI MASAYUKI

日本皮膚科学会雑誌 117 ( 1 ) 1-13 2007.01

Research paper (scientific journal), Joint Work

Synergistic pathogenic effects of combined mouse monoclonal anti-desmoglein 3 IgG antibodies on pemphigus vulgaris blister formation

Kawasaki, H. Tsunoda, K. Hata, T. Ishii, K. Yamada, T. Amagai, M.

J Invest Dermatol 126 ( 12 ) 2621-2630 2006.12

Research paper (scientific journal), Joint Work, Accepted

自己免疫性水疱症の最近の進歩：診断

AMAGAI MASAYUKI

日本皮膚科学会雑誌 116 ( 13 ) 1937-1939 2006.12

Research paper (scientific journal), Single Work

Pathogenic monoclonal antibody against desmoglein 3 augments desmoglein 3 and p38 MAPK phosphorylation in human squamous carcinoma cell line

Kawasaki, Y. Aoyama, Y. Tsunoda, K. Amagai, M. Kitajima, Y.

Autoimmunity 39 ( 7 ) 587-590 2006.11

Research paper (scientific journal), Joint Work, Accepted

T細胞受容体β鎖およびγ鎖の遺伝子再構成が検出されたlymphomatoid papulosisの1例

AMAGAI MASAYUKI

臨床皮膚科 60 ( 12 ) 1119-1122 2006.11

Research paper (scientific journal), Joint Work, Accepted

Pemphigus, bullous impetigo, and the staphylococcal scalded-skin syndrome

Stanley, J. R. Amagai, M.

N Engl J Med 355 ( 17 ) 1800-1810 2006.10

Research paper (scientific journal), Joint Work, Accepted

抗デスモグレイン1、3抗体

AMAGAI MASAYUKI

検査と技術 34 ( 11 ) 1203-1205 2006.10

Research paper (scientific journal), Single Work

抗デスモグレイン1抗体、抗デスモグレイン3抗体

AMAGAI MASAYUKI

日本医師会雑誌 135 ( 特別2 ) S313 2006.10

Research paper (scientific journal), Single Work

自己免疫と感染症の標的分子、デスモグレイン

AMAGAI MASAYUKI

日本臨床免疫学会会誌 29 ( 5 ) 325-333 2006.10

Research paper (scientific journal), Single Work, Accepted

Are desmoglein autoantibodies essential for the immunopathogenesis of pemphigus vulgaris, or just 'witnesses of disease'?

Amagai, M. Ahmed, A. R. Kitajima, Y. Bystryn, J. C. Milner, Y. Gniadecki, R. Hertl, M. Pincelli, C. Fridkis-Hareli, M. Aoyama, Y. Frusic-Zlotkin, M. Muller, E. David, M. Mimouni, D. Vind-Kezunovic, D. Michel, B. Mahoney, M. Grando, S.

Exp Dermatol 15 ( 10 ) 815 2006.10

Research paper (scientific journal), Joint Work, Accepted

Non-pathogenic anti-desmoglein 3 IgG autoantibodies in Fogo Selvagem

AMAGAI MASAYUKI

J Invest Dermatol 126 ( 9 ) 1931-1932 2006.09

Research paper (scientific journal), Single Work, Accepted

自己免疫水疱症の新展開

AMAGAI MASAYUKI

BIO Clinica 21 ( 10 ) 881-885 2006.09

Research paper (scientific journal), Single Work

自己免疫疾患パラダイムとしての天疱瘡

AMAGAI MASAYUKI

医学のあゆみ 218 ( 11 ) 933-936 2006.09

Research paper (scientific journal), Single Work

抗デスモグレイン1抗体が陽性を示した水疱性類天疱瘡の1例

AMAGAI MASAYUKI

臨床皮膚科 60 ( 10 ) 899-902 2006.09

Research paper (scientific journal), Joint Work, Accepted

BP180、BP230

AMAGAI MASAYUKI

皮膚科の臨床 48 ( 10 ) 1289-1294 2006.09

Research paper (scientific journal), Joint Work

抗デスモグレイン1、3抗体

AMAGAI MASAYUKI

皮膚科の臨床 48 ( 10 ) 1283-1287 2006.09

Research paper (scientific journal), Joint Work

Production of low titers of anti-desmoglein 1 IgG autoantibodies in some patients with staphylococcal scalded skin syndrome

Anzai, H. Stanley, J. R. Amagai, M.

J Invest Dermatol 126 ( 9 ) 2139-2141 2006.09

Research paper (scientific journal), Joint Work, Accepted

Atypical pemphigus involving the esophagus with IgG antibodies to desmoglein 3 and IgA antibodies to desmoglein 1

Inui, S. Amagai, M. Tsutsui, S. Fukuhara-Yoshida, S. Itami, S. Katayama, I.

J Am Acad Dermatol 55 ( 2 ) 354-355 2006.08

Research paper (scientific journal), Joint Work, Accepted

デスモソームカドヘリンの接着障害と水疱症

AMAGAI MASAYUKI

実験医学 24 ( 13 ) 2069-2074 2006.08

Research paper (scientific journal), Joint Work

Amagai, M. Yoshida, K. Takae, Y. Saito, H. Tanikawa, A. Nishikawa, T.

J Am Acad Dermatol 55 ( 1 ) 176-177 2006.07

Research paper (scientific journal), Joint Work, Accepted

デスモソームカドヘリンの接着障害によるヒトならびに動物の水疱症

AMAGAI MASAYUKI

生化学 78 ( 7 ) 609-614 2006.07

Research paper (scientific journal), Joint Work

IgA/IgG pemphigus: a new atypical subset of pemphigus?

Kowalewski, C. Hashimoto, T. Amagai, M. Jablonska, S. Mackiewicz, W. Wozniak, K.

Acta Derm Venereol 86 ( 4 ) 357-358 2006.07

Research paper (scientific journal), Joint Work, Accepted

SSSSと伝染性膿痂疹の発症メカニズム

AMAGAI MASAYUKI

Monthly Book Derma 114 14-19 2006.06

Research paper (scientific journal), Joint Work

新規デスモグレインDsg4に対する天疱瘡自己抗体の反応性

AMAGAI MASAYUKI

Medicina 43 ( 6 ) 947-949 2006.06

Research paper (scientific journal), Joint Work, Accepted

Intraepidermal neutrophilic IgA/IgG antidesmocollin 1 pemphigus

Heng, A. Nwaneshiudu, A. Hashimoto, T. Amagai, M. Stanley, J. R.

Br J Dermatol 154 ( 5 ) 1018-1020 2006.05

Research paper (scientific journal), Joint Work, Accepted

特集：若手研究者の養成と日本学術振興会賞　目の前にある小さな疑問

AMAGAI MASAYUKI

学術月報 59 ( 5 ) 340-341 2006.05

Research paper (scientific journal), Single Work, Accepted

増殖性天疱瘡

AMAGAI MASAYUKI

皮膚病診療 28 ( 5 ) 567-570 2006.05

Research paper (scientific journal), Joint Work, Accepted

疾患　デスモグレインの接着障害と水疱症　天疱瘡と膿痂疹

AMAGAI MASAYUKI

蛋白質・核酸・酵素 51 ( 6 ) 796-802 2006.05

Research paper (scientific journal), Joint Work, Accepted

妊娠性疱疹　産褥期に発症した症例

AMAGAI MASAYUKI

皮膚病診療 28 ( 5 ) 579-582 2006.05

Research paper (scientific journal), Joint Work, Accepted

Aleukaemic leukaemia cutis as an initial manifestation of myeloid/NK cell precursor acute leukaemia

Dekio, I. Anzai, H. Ohyama, M. Tanikawa, A. Amagai, M. Yokoyama, K. Okamoto, S. Tanaka, M.

J Eur Acad Dermatol Venereol 20 ( 4 ) 453-456 2006.04

Research paper (scientific journal), Joint Work, Accepted

新しい検査法と診断法　BP 230 ELISA法の開発について

AMAGAI MASAYUKI

臨床皮膚科 60 ( 5 ) 79-82 2006.04

Research paper (scientific journal), Joint Work, Accepted

Desmoglein endocytosis and desmosome disassembly are coordinated responses to pemphigus autoantibodies

Calkins, C. C. Setzer, S. V. Jennings, J. M. Summers, S. Tsunoda, K. Amagai, M. Kowalczyk, A. P.

J Biol Chem 281 ( 11 ) 7623-7634 2006.03

Research paper (scientific journal), Joint Work, Accepted

天疱瘡における自己抗体

AMAGAI MASAYUKI

分子リウマチ 3 ( 1 ) 28-33 2006.03

Research paper (scientific journal), Single Work, Accepted

Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid

Yoshida, M. Hamada, T. Amagai, M. Hashimoto, K. Uehara, R. Yamaguchi, K. Imamura, K. Okamoto, E. Yasumoto, S. Hashimoto, T.

J Dermatol Sci 41 ( 1 ) 21-30 2006.01

Research paper (scientific journal), Joint Work, Accepted

落葉状天疱瘡から尋常性天疱瘡に移行するとともに血清中の抗デスモグレイン1および3抗体に変動を認めた1例

AMAGAI MASAYUKI

臨床皮膚科 60 ( 1 ) 20-23 2006.01

Research paper (scientific journal), Joint Work, Accepted

A case of drug-induced hypersensitivity syndrome showing transient immunosuppression before viral reactivation during treatment for pemphigus foliaceus

Takahashi, H. Tanaka, M. Tanikawa, A. Toyohara, A. Ogo, Y. Morimoto, A. Harato, R. Kobayashi, M. Amagai, M.

Clin Exp Dermatol 31 ( 1 ) 33-35 2006.01

Research paper (scientific journal), Joint Work, Accepted

Tolerance induction by the blockade of CD40/CD154 interaction in pemphigus vulgaris mouse model

Aoki-Ota, M. Kinoshita, M. Ota, T. Tsunoda, K. Iwasaki, T. Tanaka, S. Koyasu, S. Nishikawa, T. Amagai, M.

J Invest Dermatol 126 ( 1 ) 105-113 2006.01

Research paper (scientific journal), Joint Work, Accepted

Staphylococcus hyicus exfoliative toxins selectively digest porcine desmoglein 1

Fudaba, Y. Nishifuji, K. Andresen, L. O. Yamaguchi, T. Komatsuzawa, H. Amagai, M. Sugai, M.

Microb Pathog 39 ( 5-6 ) 171-176 2005.11

Research paper (scientific journal), Joint Work, Accepted

表皮細胞間接着分子と皮膚疾患

AMAGAI MASAYUKI

Molecular Medicine 42 ( 12 ) 1351-1356 2005.11

Research paper (scientific journal), Joint Work, Accepted

Paraneoplastic pemphigus with widespread mucosal involvement

Wakahara, M. Kiyohara, T. Kumakiri, M. Ueda, T. Ishiguro, K. Fujita, T. Amagai, M. Hashimoto, T.

Acta Derm Venereol 85 ( 6 ) 530-532 2005.11

Research paper (scientific journal), Joint Work, Accepted

Cloning of swine desmoglein 1 and its direct proteolysis by Staphylococcus hyicus exfoliative toxins isolated from pigs with exudative epidermitis

Nishifuji, K. Fudaba, Y. Yamaguchi, T. Iwasaki, T. Sugai, M. Amagai, M.

Vet Dermatol 16 ( 5 ) 315-323 2005.10

Research paper (scientific journal), Joint Work, Accepted

自己免疫性水疱症の自己抗原

AMAGAI MASAYUKI

日本医師会雑誌　生涯教育シリーズ－実践　皮膚病変のみかた 134 ( 2 ) 132 2005.10

Research paper (scientific journal), Single Work, Accepted

結節性線状IgA水疱性皮膚症の1例

AMAGAI MASAYUKI

臨床皮膚科 59 ( 11 ) 1073-1075 2005.10

Research paper (scientific journal), Joint Work, Accepted

Cicatricial pemphigoid of the oropharynx after allogeneic stem cell transplantation for relapsed follicular lymphoma

Aisa, Y. Mori, T. Nakazato, T. Yamazaki, R. Yamagami, J. Amagai, M. Ikeda, Y. Okamoto, S.

Int J Hematol 82 ( 3 ) 266-269 2005.10

Research paper (scientific journal), Joint Work, Accepted

paraneoplastic pemphigus

AMAGAI MASAYUKI

皮膚病診療 27 ( 7 ) 743-748 2005.07

Research paper (scientific journal), Joint Work, Accepted

抗デスモグレイン1および3抗体

AMAGAI MASAYUKI

日本臨牀　広範囲　血液・尿検査、免疫学的検査（３） 63 ( 増7 ) 571-574 2005.07

Research paper (scientific journal), Single Work, Accepted

自己免疫性水疱症における自己抗体

AMAGAI MASAYUKI

最新医学　免疫と疾患－自己免疫と疾患－ 60 ( 増 ) 241-251 2005.06

Research paper (scientific journal), Joint Work, Accepted

In vitro keratinocyte dissociation assay for evaluation of the pathogenicity of anti-desmoglein 3 IgG autoantibodies in pemphigus vulgaris

Ishii, K. Harada, R. Matsuo, I. Shirakata, Y. Hashimoto, K. Amagai, M.

J Invest Dermatol 124 ( 5 ) 939-946 2005.05

Research paper (scientific journal), Joint Work, Accepted

自己免疫性水疱症

AMAGAI MASAYUKI

日本臨牀　臨床免疫学－基礎研究の進歩と最新の臨床－ 63 ( 増5 ) 540-546 2005.05

Research paper (scientific journal), Single Work, Accepted

Cutaneous type pemphigus vulgaris: a rare clinical phenotype of pemphigus

Yoshida, K. Takae, Y. Saito, H. Oka, H. Tanikawa, A. Amagai, M. Nishikawa, T.

J Am Acad Dermatol 52 ( 5 ) 839-845 2005.05

Research paper (scientific journal), Joint Work, Accepted

In vivo ultrastructural localization of the desmoglein 3 adhesive interface to the desmosome mid-line

Shimizu, A. Ishiko, A. Ota, T. Saito, H. Oka, H. Tsunoda, K. Amagai, M. Nishikawa, T.

J Invest Dermatol 124 ( 5 ) 984-988 2005.05

Research paper (scientific journal), Joint Work, Accepted

Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display

Payne, A. S. Ishii, K. Kacir, S. Lin, C. Li, H. Hanakawa, Y. Tsunoda, K. Amagai, M. Stanley, J. R. Siegel, D. L.

J Clin Invest 115 ( 4 ) 888-899 2005.04

Research paper (scientific journal), Joint Work, Accepted

自己抗原ノックアウトマウスモデルからB細胞トランスジェニックマウスへ

AMAGAI MASAYUKI

Molecular Medicine 42 ( 4 ) 419-424 2005.04

Research paper (scientific journal), Joint Work, Accepted

自己免疫性水疱症の免疫抑制療法

AMAGAI MASAYUKI

最新医学 60 ( 3 ) 91-96 2005.03

Research paper (scientific journal), Joint Work, Accepted

Gottron-like papules induced by hydroxyurea

Yoshida, K. Saito, M. Amagai, M. Ikeda, Y.

Clinical and Experimental Dermatology 30 ( 2 ) 191-192 2005.03

Research paper (scientific journal), Joint Work, Accepted

Trousseau症候群　—胃癌を伴い、Trousseau症候群が疑われた多発性皮膚壊死の1例—

AMAGAI MASAYUKI

皮膚病診療 27 ( 3 ) 273-276 2005.03

Research paper (scientific journal), Joint Work, Accepted

Decline of anti-desmoglein 1 IgG ELISA scores by withdrawal of D-penicillamine in drug-induced pemphigus foliaceus

Nagao, K. Tanikawa, A. Yamamoto, N. Amagai, M.

Clinical and Experimental Dermatology 30 ( 1 ) 43-45 2005.01

Research paper (scientific journal), Joint Work, Accepted

Elephantiasis nostrasの1例

AMAGAI MASAYUKI

臨床皮膚科 53 ( 13 ) 1170-1172 2004.12

Research paper (scientific journal), Joint Work, Accepted

自己抗原ノックアウトマウスを用いたモデルマウスの作成と応用

AMAGAI MASAYUKI

Medical Science Digest 30 ( 12 ) 480-482 2004.11

Research paper (scientific journal), Joint Work, Accepted

Parallel fluctuation of anti-desmoglein 3 and anti-BP180 autoantibody titres in a patient with bullous pemphigoid

Takahashi, H. Anzai, H. Suzuki, Y. Tanikawa, A. Amagai, M. Nishikawa, T.

Clinical and Experimental Dermatology 29 ( 6 ) 608-611 2004.11

Research paper (scientific journal), Joint Work, Accepted

Defining the pathogenic involvement of desmoglein 4 in pemphigus and staphylococcal scalded skin syndrome

Nagasaka, T. Nishifuji, K. Ota, T. Whittock, N. V. Amagai, M.

The Journal of Clinical Investigation 114 ( 10 ) 1484-1492 2004.11

Research paper (scientific journal), Joint Work, Accepted

Updates on autoimmune skin bullous diseases

AMAGAI MASAYUKI

Japan Medical Association Journal 47 ( 11 ) 514-519 2004.11

Research paper (scientific journal), Single Work, Accepted

Exclusion of the TAP1 and TAP2 genes within the HLA class II region as candidate susceptibility genes to pemphigus in the Japanese population

Niizeki, H. Kumagai, S. Kanagawa, S. Amagai, M. Yamashina, Y. Asada, H. Nishikawa, T. Miyagawa, S.

Journal of Dermatological Science 36 ( 2 ) 122-124 2004.11

Research paper (scientific journal), Joint Work, Accepted

抗デスモグレイン自己免疫疾患・天疱瘡の病因解明　−この10年の進歩−

AMAGAI MASAYUKI

細胞 36 ( 12 ) 477-480 2004.10

Research paper (scientific journal), Single Work, Accepted

Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism

Ota, T. Aoki-Ota, M. Tsunoda, K. Simoda, K. Nishikawa, T. Amagai, M. Koyasu, S.

International Immunology 16 ( 10 ) 1487-1495 2004.10

Research paper (scientific journal), Joint Work, Accepted

「進化」する疾患モデル動物　自己抗原ノックアウトマウスを用いた天疱瘡モデルマウス

AMAGAI MASAYUKI

細胞工学 23 ( 10 ) 1198-1201 2004.09

Research paper (scientific journal), Joint Work, Accepted

Conformational epitope mapping of antibodies against desmoglein 3 in experimental murine pemphigus vulgaris

Anzai, H. Fujii, Y. Nishifuji, K. Aoki-Ota, M. Ota, T. Amagai, M. Nishikawa, T.

Journal of Dermatological Science 35 ( 2 ) 133-142 2004.08

Research paper (scientific journal), Joint Work, Accepted

A mouse model of pemphigus vulgaris by adoptive transfer of naive splenocytes from desmoglein 3 knockout mice

Aoki-Ota, M. Tsunoda, K. Ota, T. Iwasaki, T. Koyasu, S. Amagai, M. Nishikawa, T.

British Journal of Dermatology 151 ( 2 ) 346-354 2004.08

Research paper (scientific journal), Joint Work, Accepted

コンピューターキーボードによる角化症

AMAGAI MASAYUKI

皮膚病診療 26 ( 6 ) 682-685 2004.06

Research paper (scientific journal), Joint Work, Accepted

水疱性および瘢痕性類天疱瘡の眼合併症

AMAGAI MASAYUKI

日本眼科紀要 55 ( 5 ) 374-377 2004.05

Research paper (scientific journal), Joint Work, Accepted

IgG binds to desmoglein 3 in desmosomes and causes a desmosomal split without keratin retraction in a pemphigus mouse model

Shimizu, A. Ishiko, A. Ota, T. Tsunoda, K. Amagai, M. Nishikawa, T.

Journal of Investigative Dermatology 122 ( 5 ) 1145-1153 2004.05

Research paper (scientific journal), Joint Work, Accepted

Hydroxyureaによる皮膚症状

AMAGAI MASAYUKI

臨床皮膚科 58 ( 5 ) 20-23 2004.04

Research paper (scientific journal), Joint Work, Accepted

ELISAによる天疱瘡自己抗体価測定法

AMAGAI MASAYUKI

臨床皮膚科 58 ( 5 ) 84-88 2004.04

Research paper (scientific journal), Single Work, Accepted

融合性細網状乳頭腫症の1例

NISHIKAWA TAKEJI

臨床皮膚科 58 ( 3 ) 246-248 2004.03

Research paper (scientific journal), Joint Work

ブドウ球菌性熱傷様皮膚症候群：SSSS

AMAGAI MASAYUKI

小児科診療 67 ( 3 ) 391-395 2004.03

Research paper (scientific journal), Single Work

水疱性疾患の自己抗体

AMAGAI MASAYUKI

臨床検査 48 ( 3 ) 283-288 2004.03

Research paper (scientific journal), Single Work

Enzymatic and molecular characteristics of the efficiency and specificity of exfoliative toxin cleavage of desmoglein 1

'Hanakawa Y, Schechter N M, Lin C, Nishifuji K, Amagai M, Stanley J R'

Journal of Biological Chemistry 279 ( 7 ) 5268-5277 2004.02

Research paper (scientific journal), Joint Work

Superficial mucoceleの1例

AMAGAI MASAYUKI

臨床皮膚科 58 ( 2 ) 157-159 2004.02

Research paper (scientific journal), Joint Work

炎症性皮膚疾患の動物モデル：天疱瘡

AMAGAI MASAYUKI

アレルギー科 17 ( 2 ) 136-140 2004.02

Research paper (scientific journal), Single Work

A mouse with an active disease as a model for pemphigus

AMAGAI MASAYUKI

Annales de Dermatologie et de Venereologie 130 ( 12 ) 1176-1179 2003.12

Research paper (scientific journal), Single Work

Conformational epitope mapping and IgG subclass distribution of desmoglein 3 in paraneoplastic pemphigus

'Futei Y, Amagai M, Hashimoto T, Nishikawa T'

Journal of the American Academy of Dermatology 49 ( 6 ) 1023-1028 2003.12

Research paper (scientific journal), Joint Work

天谷雅行

AMAGAI MASAYUKI

日本皮膚科学会雑誌 113 ( 13 ) 1947-1948 2003.12

Research paper (scientific journal), Single Work

230 kDa and 190 kDa proteins in addition to desmoglein 1 as immunological targets in a subset of pemphigus foliaceus with a combined cell surface and basement membrane zone immune staining pattern

'Karlhofer F M, Hashimoto T, Slupetzky K, Kiss M, Liu Y, Amagai M, Pieczkowski F, Foedinger D, Kirnbauer R, Stingl G'

Experimental Dermatology 12 ( 5 ) 646-654 2003.10

Research paper (scientific journal), Joint Work

Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression

'Nishifuji K, Amagai M, Nishikawa T, Iwasaki T'

Veterinary Immunology and Immunopathology 95 ( 39511 ) 177-182 2003.10

Research paper (scientific journal), Joint Work

自己免疫性水疱症

天谷雅行

皮膚科の臨床 45 ( 11 ) 1343-1349 2003.10

Research paper (scientific journal), Single Work

A case of generalized pustular psoriasis treated with topical tacrolimus

'Ishiko A, Yokoyama T, Tanikawa A, Amagai M, Nagao K'

Archives of Dermatology 139 ( 9 ) 1219 2003.09

Research paper (scientific journal), Joint Work

Cloning of canine desmoglein 3 and immunoreactivity of serum antibodies in human and canine pemphigus vulgaris with its extracellular domains

'Nishifuji K, Amagai M, Ota T, Nishikawa T, Iwasaki T'

Journal of Dermatological Science 32 ( 3 ) 181-191 2003.09

Research paper (scientific journal), Joint Work

デスモグレインテスト「Dsg3」「Dsg1」について

AMAGAI MASAYUKI

皮膚病診療 25 ( 9 ) 1058-1060 2003.09

Research paper (scientific journal), Single Work

天疱瘡

AMAGAI MASAYUKI

医薬の門 43 ( 4 ) 100-101 2003.08

Research paper (scientific journal), Single Work

No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases

'Ota T, Amagai M, Watanabe M, Nishikawa T'

Journal of Dermatological Science 32 ( 2 ) 137-141 2003.08

Research paper (scientific journal), Single Work

黄色ブドウ球菌毒素ETの標的分子

天谷雅行

Medical Science Digest 29 ( 7 ) 2003.07

Research paper (scientific journal), Single Work

リベドを呈したサルコイドーシスの1例

戸田素子，　小林誠一郎，　原藤玲，　石河晃，　天谷雅行，　飯塚秀子，　佐藤慎二，　天谷雅行

臨床皮膚科 57 ( 8 ) 712-715 2003.07

Research paper (scientific journal), Joint Work

Comparative study of autoantigen profile between Colombian and Brazilian types of endemic pemphigus foliaceus by various biochemical and molecular biological techniques

'Hisamatsu Y, Abreu Velez A M, Amagai M, Ogawa M M, Kanzaki T, Hashimoto T'

Journal of Dermatological Science 32 ( 1 ) 33-41 2003.06

Research paper (scientific journal), Joint Work

自己免疫水疱症の最新情報

AMAGAI MASAYUKI

日本医師会雑誌 129 ( 9 ) 1425-1429 2003.05

Research paper (scientific journal), Single Work

'最新の臨床検査　−天疱瘡の新しい血清診断法：Dsg1, Dsg3 ELISA法−'

AMAGAI MASAYUKI

SRL宝函 27 ( 2 ) 66-72 2003.05

Research paper (scientific journal), Single Work

Suppression of the immune response against exogenous desmoglein 3 in desmoglein 3 knockout mice: an implication for gene therapy

Ohyama Manabu, Ota Takayuki, Aoki Miyo, Tsunoda Kazuyuki, Harada Reiko, Koyasu Shigeo, Nishikawa Takeji, Amagai Masayuki

The journal of investigative dermatology 120 ( 4 ) 610-615 2003.04

Research paper (scientific journal), Joint Work

Suppression of the immune response against exogenous desmoglein 3 in desmoglein 3 knockout mice: An implication for gene therapy

'Ohyama M, Ota T, Aoki M, Tsunoda K, Harada R, Koyasu S, Nishikawa T, Amagai M'

Journal of Investigative Dermatology 120 ( 4 ) 610-615 2003.04

Research paper (scientific journal), Joint Work

Neonatal pemphigus vulgaris: IgG4 autoantibodies to desmoglein 3 induce skin blisters in newborns

'Parlowsky T, Welzel J, Amagai M, Zillikens D, Wygold T'

Journal of the American Academy of Dermatology 48 ( 4 ) 623-625 2003.04

Research paper (scientific journal), Joint Work

Desmoglein as a target in autoimmunity and infection

Amagai Masayuki

Journal of the American Academy of Dermatology 48 ( 2 ) 244-252 2003.02

Research paper (scientific journal), Single Work

Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3

Tsunoda Kazuyuki, Ota Takayuki, Aoki Miyo, Yamada Taketo, Nagai Tetsuo, Nakagawa Taneaki, Koyasu Shigeo, Nishikawa Takeji, Amagai Masayuki

Journal of immunology 170 ( 4 ) 2170-2178 2003.02

Research paper (scientific journal), Joint Work

Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein3

Tsunoda Kazuyuki, Ota Takayuki, Aoki Miyo, Yamada Taketo, Nagai Tetsuo, Nakagawa Taneaki, Koyasu Shigeo, Nishikawa Takeji, Amagai Masayuki

Jounal of immunology 170 ( 4 ) 2170-2178 2003.02

Research paper (scientific journal), Joint Work

浸潤の強い紅斑を主症状とした落葉状天疱瘡の1例

Kanaida Kazuko, Shishido Etsuko, Mizushima Junichi, Ishiguro Naoko, Kawashima Makoto, Amagai Masayuki

臨床皮膚科 57 ( 1 ) 53-56 2003.01

Research paper (scientific journal), Joint Work

Differential effects of desmoglein 1 and desmoglein 3 on desmosome formation

Hanakawa Yasushi, Amagai Masayuki, Shirakata Yuji, Yahata Yoko, Tokumaru Sho, Yamasaki Kenshi, Tohyama Mikiko, Sayama Koji, Hashimoto Koji

Journal of investigative dermatology 119 ( 6 ) 1231-1236 2002.12

Research paper (scientific journal), Joint Work

BP180 ELISA using bacterial recombinant NC16a protein as a diagnostic and monitoring tool for bullous pemphigoid

Kobayashi Masakazu, Amagai Masayuki, Kuroda-Kinoshita Keiko, Hashimoto Takashi, Shirakata Yuji, Hashimoto Koji, Nishikawa Takeji

Journal of dermatological science 30 ( 3 ) 224-232 2002.12

Research paper (scientific journal), Joint Work

尋常性天疱瘡の1例―ELISA法による病勢モニタリング

Toda Motoko, Kobayashi Masakazu, Anzai Hidemi, Tei Shoui, Tanikawa Akiko, Amagai Masayuki, Nishikawa Takeji

臨床皮膚科 56 ( 12 ) 1023-1026 2002.11

Research paper (scientific journal), Joint Work

IgG/IgA pemphigus with IgG and IgA antidesmoglein 1 antibodies detected by enzyme-linked immunosorbent assay

Oiso N, Yamashita C, Yoshioka K, Amagai Masayuki, Komai A, Nagata Y, Hashimoto T, Ishii M

British journal of dermatology 147 ( 5 ) 1012-1017 2002.11

Research paper (scientific journal), Joint Work

A case of IgA pemphigus successfully treated with acitretin

Ruiz-Genao DP, Hernandez-Nunez A, Hashimoto T, Amagai Masayuki, Fernandez-Herrera J, Garcia-Diez A

British journal of dermatology 147 ( 5 ) 1041-1042 2002.11

Research paper (scientific journal), Joint Work

表皮細胞接着と皮膚疾患

Amagai Masayuki

日本皮膚科学会雑誌 112 ( 12 ) 1569-1575 2002.11

Research paper (scientific journal), Single Work

小膿疱を呈したアンダームによる接触皮膚炎

Ichikawa Naoko, Anzai Hidemi, Amagai Masayuki

皮膚病診療 24 ( 10 ) 1115-1118 2002.10

Research paper (scientific journal), Joint Work

Identification of the Staphylococcus aureus etd pathogenicity island which encodes a novel exfoliative toxin, ETD, and EDIN-B

Yamaguchi Takayuki, Nishifuji Koji, Sasaki Megumi, Fudaba Yasuyuki, Aepfelbacher Martin, Takata Takashi, Ohara Masaru, Komatsuzawa Hitoshi, Amagai Masayuki, Sugai Motoyuki

Infection & immunity 70 ( 10 ) 5835-5845 2002.10

Research paper (scientific journal), Joint Work

Neonatal pemphigus vulgaris with extensive mucocutaneous lesions from a mother with oral pemphigus vulgaris

Campo-Voegeli A, Muniz F, Casals M, Mascaro JM, Garcia F, Arimany JL, Amagai Masayuki, Camps A

British journal of dermatology 147 ( 4 ) 801-805 2002.10

Research paper (scientific journal), Joint Work

The ultrastructural changes of mice actively producing antibodies to desmoglein 3 parallel those in patients with pemphigus vulgaris

'Shimizu Atsushi, Ishiko Akira, Ota Takayuki, Tsunoda Kazuyuki, Koyasu Shigeo, Amagai Masayuki, Nishikawa Takeji'

Archives of dermatological research 294 ( 7 ) 318-323 2002.09

Research paper (scientific journal), Joint Work

Pemphigus as a paradigm of autoimmunity and cell adhesion

Amagai Masayuki

Keio journal of medicine 51 ( 3 ) 133-139 2002.09

Research paper (scientific journal), Single Work

天疱瘡の分子生物学

Amagai Masayuki

現代医療 34 ( 8 ) 1846-1851 2002.08

Research paper (scientific journal), Single Work

Monitoring disease activity in pemphigus with enzyme-linked immunosorbent assay using recombinant desmogleins 1 and 3

Cheng SW, Kobayashi Masakazu, Tanikawa Akiko, Kinoshita-Kuroda Keiko, Amagai Masayuki, Nishikawa Takeji

British journal of dermatology 147 ( 2 ) 261-265 2002.08

Research paper (scientific journal), Joint Work

ステロイドパルス療法が有効であった抗ラミニン5瘢痕性類天疱瘡の1例

Inada Sachiko, Kobayashi Seiichirou, Ooyama Manabu, Tanikawa Akiko, Amagai Masayuki, Matsui Chihiro, Morohashi Masaaki, Tsubota Kazuo

臨床皮膚科 56 ( 9 ) 731-734 2002.08

Research paper (scientific journal), Joint Work

尋常性天疱瘡と水疱性類天疱瘡の合併例

Asano Ayumi, Amagai Masayuki

臨床皮膚科 56 ( 8 ) 600-603 2002.07

Research paper (scientific journal), Joint Work

Immunological and histopathological characterization of active disease mouse model for pemphigus vulgaris

Ohyama Manabu, Amagai Masayuki, Tsunoda Kazuyuki, Ota Takayuki, Koyasu Shigeo, Hata Jun-ichi, Umezawa Akihiro, Nishikawa Takeji

The journal of investigative dermatology 118 ( 1 ) 199-204 2002.07

Research paper (scientific journal), Joint Work

Molecular mechanisms of blister formation in bullous impetigo and staphylococcal scalded skin syndrome

Hanakawa Yasushi, Schechter Norman M, Lin Chenyan, Garza Luis, Li Hong, Yamaguchi Takayuki, Fudaba Yasuyuki, Nishifuji Koji, Sugai Motoyuki, Amagai Masayuki, Stanley John R

Journal of clinical investigation 110 ( 1 ) 53-60 2002.07

Research paper (scientific journal), Joint Work

Paraneoplastic pemphigus associated with Castleman's disease and asymptomatic bronchiolitis obliterans

Fujimoto Wataru, Kanehiro Arihiko, Kuwamoto-Hara Kazue, Saitoh Mari, Nakakita Takashi, Amagai Masayuki, Arata Jiro, Iwatsuki Keiji

European journal of dermatology 12 ( 4 ) 355-359 2002.07

Research paper (scientific journal), Joint Work

天疱瘡とデスモグレイン

Amagai Masayuki

Medical technology 30 ( 7 ) 762-763 2002.07

Research paper (scientific journal), Single Work

Mycophenolate mofetilが奏功した尋常性天疱瘡の1例

Anzai Hidemi, Amagai Masayuki, Ooyama Manabu, Tanikawa Akiko, Tanaka Masaru, Nishikawa Takeji

臨床皮膚科 56 ( 7 ) 498-502 2002.06

Research paper (scientific journal), Joint Work

四肢に多発した壊疽性膿皮症の1例

Kobayashi Seiichirou, Tajima Maiko, Miyake Ayako, Hakuno Megumi, Tanikawa Akiko, Amagai Masayuki, Nishikawa Takeji, HaッTori Kuniyuki

臨床皮膚科 56 ( 7 ) 529-531 2002.06

Research paper (scientific journal), Joint Work

13歳女児に発症した尋常性天疱瘡の1例

Suzuki Kenji, Shimizu Satoshi, Suga Masako, Itou Masahiko, Kubota Hideaki, Miyake Satoru, Gotou Kyouko, Amagai Masayuki

日本口腔粘膜学会雑誌 8 ( 1 ) 37-42 2002.06

Research paper (scientific journal), Joint Work

Staphylococcal exfoliative toxin B specifically cleaves desmoglein 1

Amagai Masayuki, Yamaguchi Takayuki, Hanakawa Yasushi, Nishifuji Koji, Sugai Motoyuki, Stanley John R

Journal of investigative dermatology 118 ( 5 ) 845-850 2002.05

Research paper (scientific journal), Joint Work

Duhring疱疹状皮膚炎（granular IgA type）の日本人女性例

Emori Yuuichi, Inazumi Toyoko, Kimura Shunji

臨床皮膚科 56 ( 6 ) 428-430 2002.05

Research paper (scientific journal), Joint Work

Brown palm pseudochromhidrosis

Yoshida Rie, Kobayashi Seiichiro, Amagai Masayuki, Tanaka Masaru

Contact dermatitis 46 ( 4 ) 237-238 2002.04

Research paper (scientific journal), Joint Work

ブドウ球菌性熱傷様皮膚症候群（SSSS）におけるデスモグレイン1の関与

Amagai Masayuki

臨床皮膚科 56 ( 5 ) 58-61 2002.04

Research paper (scientific journal), Single Work

Immunologic and histopathologic characterization of active disease model for pemphigus vulgaris

Ohyama Manabu, Amagai Masayuki, Tsunoda Kazuyuki, Ota Takayuki, Hata Jun-ichi, Umezawa Akihiro, Nishikawa Takeji

Journal of Inverstigative Dermatology 118 ( 1 ) 199-204 2002.03

Research paper (scientific journal), Joint Work

Pathogenic autoantibody production requires loss of tolerance against desmoglein 3 in both T and B cells in experimental pemphigus vulgaris

Tsunoda Kazuyuki, Ota Takayuki, Suzuki Harumi, Ohyama Manabu, Nagai Tetsuo, Nishikawa Takeji, Amagai Masayuki, Koyasu Shigeo

European Journal of Immunology 32 ( 3 ) 627-633 2002.03

Research paper (scientific journal), Joint Work

Neutrophilic eccrine hidradenitisの1例

Kimoto Masayuki，Futei Yuuko，Kawahara Yoshie，Tanikawa Akiko，Amagai Masayuki

臨床皮膚科 56 ( 3 ) 203-205 2002.03

Research paper (scientific journal), Joint Work

Pathogenic autoantibody production requires loss of tolerance against desmoglein 3 in both T and B cells in experimental pemphigus vulgaris

Tsunoda K, Ota T, Suzuki H, Ohyama M, Nagai T, Nishikawa T, Amagai M, Koyasu S

European Journal of Immunology 32 ( 3 ) 627-633 2002.03

Research paper (scientific journal), Joint Work

自己免疫性水疱症の最新知見

Amagai Masayuki

日本臨床免疫学会会誌 25 ( 1 ) 28-32 2002.02

Research paper (scientific journal), Single Work

nduction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3

Tsunoda Kazuyuki, Ota Takayuki, Aoki Miyo, Yamada Taketo, Nagai Tetsuo, Nakagawa Takeaki, Koyasu Shigeo, Nishikawa Takeji, Amagai Masayuki

Journal of Immunology 170 ( 4 ) 2170-217 2002.02

Research paper (scientific journal), Joint Work

Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3

Tsunoda Kazuyuki, Ota Takyuki, Suzuki Harumi, Ohyama Manabu, Nagai Tetsuo, Nishikawa Takeji, Amagai Masayuki, Koyasu Shigeo

Journal of Immunology 170 ( 4 ) 2170-2178 2002.02

Research paper (scientific journal), Joint Work

Lichen aureusの1例

Fujimoto Atsushi，Murata Takayuki，Amagai Masayuki

臨床皮膚科 56 ( 2 ) 124-126 2002.02

Research paper (scientific journal), Joint Work

Immunologic and histopathologic characterization of active disease model mouse for pemphigus vulgaris

Ohyama Manabu, Amagai Masayuki, Tsunoda Kazuyuki, Ota Takayuki, Koyasu Shigeo, Hata Jun-ichi, Umezawa Akihiro, Nishikawa Takeji

Journal of Investigative Dermatology 118 ( 1 ) 199-204 2002.01

Research paper (scientific journal), Joint Work

Immunologic and histopathologic characterization of an active disease mouse model for pemphigus vulgaris

Ohyama Manabu, Amagai Masayuki, Tsunoda Kazuyuki, Ota Takayuki, Koyasu Shigeo, Hata Jun-ichi, Umezawa Akihiro, Nihsikawa Takeji

Journal of Investigative Dermatology 118 ( 1 ) 199-204 2002.01

Research paper (scientific journal), Joint Work

Immunologic and histopathologic characterization of an active disease mouse model for pemphigus vulgaris

Ohyama M, Amagai M, Tsunoda K, Ota T, Koyasu S, Hata J, Umezawa A, Nishikawa T

Journal of Investigative Dermatology 118 ( 1 ) 199-204 2002.01

Research paper (scientific journal), Joint Work

Immunologic and histopathologic characterization of active disease model mouse for pemphigus vulgaris

Ohyama M, Amagai M, Tsunoda K, Ota T, Koyasu S, Hata J, Umezawa A, Nishikawa T

Journal of Investigative Dermatology 118 ( 1 ) 199-204 2002

Research paper (scientific journal), Joint Work

Pathogenic autoantibody production requires loss of tolerance against desmoglein 3 in both T and B cells in experimental pemphigus vulgaris

Tsunoda Kazuyuki, Ota Takayuki, Suzuki Harumi, Ohyama Manabu, Nagai Tetsuo, Nishikawa Takeji, Amagai Masayuki, Koyasu Shigeo

European journal of immunology 32 627-633 2002

Research paper (scientific journal), Joint Work

自己抗原ノックアウトマウスを用いた新たな自己免疫疾患モデル動物

Amagai Masayuki

免疫2002 38 ( 臨時増刊 ) 190-196 2001.11

Research paper (scientific journal), Single Work

Dominant autoimmune epitopes recognized by pemphigus antibodies map to the N-terminal adhesive region of desmogleins

Sekiguchi M, Futei Y, Fujii Y, Iwasaki T, Nishikawa T, Amagai M

Journal of Immunology 167 ( 9 ) 5439-5448 2001.11

Research paper (scientific journal), Joint Work

Pemphigus vulgaris with autoantibodies to desmoplakin

Kim S-C, Chung YL, Kim J, Cho NJ, Amagai M

British Journal of Dermatology 145 ( 5 ) 838-840 2001.11

Research paper (scientific journal), Joint Work

自己免疫のモデル疾患としての天疱瘡

Amagai Masayuki

日本皮膚科学会雑誌 111 ( 12 ) 1769-1771 2001.11

Research paper (scientific journal), Single Work

A case of pemphigus foliaceus which occurred after five years of remission from pemphigus vulgaris

Kimoto M, Ohyama M, Hata Y, Amagai M, Nishikawa T

Dermatology 203 ( 2 ) 174-176 2001.10

Research paper (scientific journal), Joint Work

粘膜と皮膚に生じる免疫疾患

Tsunoda Kazuyuki，Amagai Masayuki

医学のあゆみ 199 ( 1 ) 103-106 2001.10

Research paper (scientific journal), Joint Work

D-ペニシラミン投与中の汎発性強皮症患者に生じた尋常性天疱瘡の1例

Hirota Tomoko，Mizushima Junichi，Ishiguro Naoko，Kawashima Makoto，Amagai Masayuki

臨床皮膚科 55 ( 9 ) 707-709 2001.08

Research paper (scientific journal), Joint Work

Pemphigus foliaceus with prominent neutrophilic pustules

Matsuo K, Komai A, Ishii K, Futei Y, Amagai M, Deguchi H, Danno K, Hashimoto T

British Journal of Dermatology 145 ( 1 ) 132-136 2001.07

Research paper (scientific journal), Joint Work

多彩な組織像を呈した有棘細胞癌の1例―細胞間接着分子発現の免疫組織化学的検討も含めて―

Saitou Masataka，Kawahara Yoshie，Nonaka Shuuko，Oohata Yoshiyuki，Tanikawa Akiko，Amagai Masayuki，Tanaka Masaru，Shimizu Hiroshi

臨床皮膚科 55 ( 8 ) 627-630 2001.07

Research paper (scientific journal), Joint Work

妊娠中に発症した落葉状天疱瘡の1例

Saitou Hitoshi，Kai Misaki，Amagai Masayuki

臨床皮膚科 55 ( 7 ) 495-497 2001.06

Research paper (scientific journal), Joint Work

Detection of IgA autoantibodies to desmogleins by an enzyme-linked immunosorbent assay: the presence of new minor subtypes of IgA pemphigus

Hashimoto T, Komai A, Futei Y, Nishikawa T, Amagai M

Archives of Dermatology 137 ( 6 ) 735-738 2001.06

Research paper (scientific journal), Joint Work

A case of generalized Hailey-Hailey disease with fatal liver injury

Amagai M, Kobayashi M, Wakabayashi K, hakuno M, Hashiguchi A, Nishikawa T, Hata J

Keio Journal of Medicine 50 ( 2 ) 109-116 2001.06

Research paper (scientific journal), Joint Work

A case of generalized Hailey-Hailey disease with fatal liver injury

Amagai M, Kobayashi M, Wakabayashi K, Hakuno M, Hashiguchi A, Nishikawa T, Hata J

The Keio Journal of Medicine 50 ( 2 ) 109-116 2001.06

Research paper (scientific journal), Joint Work

Paraneoplastic pemphigus: an association with fludarabine?

Gooptu C, Littlewood T, Fritsch P, Lyon CC, Carmichael AJ, Oliwiecki S, MacWhannel A, Amagai M, Hashimoto T, Dean D, Allen J, Wojnarowska F

British Journal of Dermatology 144 ( 6 ) 1255-1261 2001.06

Research paper (scientific journal), Joint Work

The clinical transition between pemphigus foliaceus and pemphigus vulgaris correlates well with the changes in autoantibody profile assessed by an enzyme-linked immunosorbent assay

Komai A, Amagai M, Ishii K, Nishikawa T, Chorzelski T, Matsuo I, Hashimoto T

British Journal of Dermatology 144 ( 6 ) 1177-1182 2001.06

Research paper (scientific journal), Joint Work

黄色ブドウ球菌毒素と皮膚疾患の最前線

Amagai Masayuki

実験医学 19 ( 8 ) 1004-1007 2001.06

Research paper (scientific journal), Single Work

世界の皮膚科学者　Dr. Michael Hertl

Amagai Masayuki

西日本皮膚科 63 ( 3 ) 325-326 2001.06

Research paper (scientific journal), Single Work

自己免疫疾患治療評価モデル

Amagai Masayuki

組織培養工学 27 ( 7 ) 268-271 2001.06

Research paper (scientific journal), Single Work

天疱瘡モデルマウス

Amagai Masayuki

病理と臨床 19 ( 6 ) 655-659 2001.06

Research paper (scientific journal), Single Work

デスモグレインKOマウスを用いた天疱瘡モデルマウスの作製

Amagai Masayuki

臨床免疫 35 ( 6 ) 758-762 2001.06

Research paper (scientific journal), Single Work

Predominant IgG4 subclass in autoantibodies of pemphigus vulgaris and foliaceus

Futei Y, Amagai M, Ishii K, Kuroda-Kinoshita K, Ohya K, Nishikawa T

Journal of Dermatological Science 26 ( 1 ) 55-61 2001.05

Research paper (scientific journal), Joint Work

著明な水疱形成を認めた持久性隆起性紅斑の1例

Yamagami Jun，Futei Yuuko，Tanikawa Akiko，Amagai Masayuki，Tanaka Masaru，Nishikawa Takeji

臨床皮膚科 55 ( 6 ) 396-398 2001.05

Research paper (scientific journal), Joint Work

Clinical phenotype and anti-desmoglein autoantibody profile in paraneoplastic pemphigus

Ohyama M, Amagai M, Hashimoto T, Nousari HC, Anhalt GJ, Nishikawa T

Journal of American Academy of Dermatology 44 ( 4 ) 593-598 2001.04

Research paper (scientific journal), Joint Work

Benign symmetrical lipomatosis

Emori Yuuichi，Murata Takayuki，Amagai Masayuki

皮膚病診療 23 ( 4 ) 385-388 2001.04

Research paper (scientific journal), Joint Work

Pemphigus: Is there another half of the story?

Stanley JR, Nishikawa T, Diaz LA, Amagai M

Journal of Investigative Dermatology 116 ( 4 ) 489-490 2001.04

Research paper (scientific journal), Joint Work

Keyboard wrist pad

Tanaka M, Fujimoto A, Kobayashi S, Hata Y, Amagai M

Contact Dermatitis 44 ( 4 ) 253-254 2001.04

Research paper (scientific journal), Joint Work

皮膚の自己免疫疾患―天疱瘡

Amagai Masayuki

実験医学 19 ( 5 ) 708-712 2001.03

Research paper (scientific journal), Single Work

Eosinophilic pustular folliculitis (Ofuji's disease): indomethacin as first choice of treatment

Ota T, Hata Y, Tanikawa A, Amagai M, Tanaka M, Nishikawa T

Clinical Experimental Dermatology 26 ( 2 ) 179-181 2001.03

Research paper (scientific journal), Joint Work

Allergic contact dermatitis from betamethasone valerate in Japan

Tajima M, Murata T, Suzuki Y, Tanikawa A, Amagai M, Tanaka M

Clinical Experimental Dermatology 26 ( 2 ) 220-221 2001.03

Research paper (scientific journal), Joint Work

Imuunoreactivity against intracellular domains of desmogleins in pemphigus

Ohata Y, Amagai M, Ishii K, Hashimoto K

Journal of Dermatological Science 25 ( 1 ) 72-77 2001.01

Research paper (scientific journal), Joint Work

自己免疫性皮膚疾患における自己抗原

Amagai Masayuki

臨床化学 29 ( 4 ) 175-183 2000.12

Research paper (scientific journal), Single Work

Rapid response of IgA pemphigus of subcorneal pustular dermatosis type to treatment with isotretinoin

Gruss C, Zillikens D, Hashimoto T, Amagai M, Kroiss M, Vogt T, Landthaler M, Stolz W

Journal of the American Academy of Dermatology 43 ( 5 ) 923-926 2000.11

Research paper (scientific journal), Joint Work

Toxin in bullous impetigo and staphylococcal scalded skin syndrome targets desmoglein 1

Amagai M, Matsuyoshi N, Wang ZH, Andl C, Stanley JR

Nature Medicine 6 ( 11 ) 1275-1277 2000.11

Research paper (scientific journal), Joint Work

Use of domain-swapped molecules for conformational epitope mapping of desmoglein 3 in pemphigus vulgaris

Futei Y, Amagai M, Sekiguchi M, Nishifuji K, Fujii Y, Nishikawa T

The Journal of Investigative Dermatology 115 ( 5 ) 829-834 2000.11

Research paper (scientific journal), Joint Work

ハムスターおよびチンチラより感染したTrichophyton mentagrophytesによるケルスス禿瘡と体部白癬

Hata Yasuki，Amagai Masayuki，Naka Wataru，Harada Reiko，Nishikawa Takeji

日本医真菌学会雑誌 41 ( 4 ) 269-273 2000.10

Research paper (scientific journal), Joint Work

特集：外来診療に必要な皮膚科検査マニュアル―水疱症に関する検査―

Amagai Masayuki

Monthly Book Derma 41 105-110 2000.10

Research paper (scientific journal), Single Work

天疱瘡の分子免疫学―抗原解析からマウスモデルへ―

Amagai Masayuki

西日本皮膚科 62 ( 5 ) 577-580 2000.10

Research paper (scientific journal), Single Work

天疱瘡の発症機序

Amagai Masayuki

日本皮膚科学会雑誌 110 ( 12 ) 1836-1837 2000.10

Research paper (scientific journal), Single Work

皮膚疾患のプライマリケア

Kobayashi Masakazu，Amagai Masayuki

レジデントノート 2 ( 4 ) 129-140 2000.10

Research paper (scientific journal), Joint Work

Thymoma with pemphigus foliaceus

Takeshita K, Amano M, Shimizu T, Oyamada Y, Abiko T, Kobayashi K, Futei Y, Amagai M, Kuramochi S, Asano K, Yamaguchi K

Internal Medicine 39 ( 9 ) 742-747 2000.09

Research paper (scientific journal), Joint Work

Clinical and immunpathological findings in 48 patients with pemphigus

Hahn K, Kippes W, Amagai M, Rzany B, Brocker E-B, Zillikens D

Hautarzt 51 ( 9 ) 670-677 2000.09

Research paper (scientific journal), Joint Work

尋常性天疱瘡

Amagai Masayuki

皮膚科の臨床 42 ( 10 ) 1486-1487 2000.09

Research paper (scientific journal), Single Work

一過性に経過した線状IgA水疱性皮膚症の1例

Saito Hitoshi，Kai Misaki，Futei Yuko，Amagai Masayuki

臨床皮膚科 54 ( 10 ) 809-812 2000.09

Research paper (scientific journal), Joint Work

Thymoma with pemphigus foliaceus

Takeshita Kei, Amano M, Shimizu T, Oyamada Yoshitaka, Abiko T, Kobayashi Koichi, Futei Y, Amagai M, Kuramochi S, Yamaguchi Kazuhiro

Internal Medicine 39 ( 9 ) 742-747 2000.09

Research paper (scientific journal), Joint Work

Towards a better understanding of pemphigus autoimmunity

Amagai M

The British Journal Dermatology 143 ( 2 ) 237-243 2000.08

Research paper (scientific journal), Single Work

Production of the entire extracellular domain of BP180 (type XVII collagen) by baculovirus expression

Hata Y, Fujii Y, Tsunoda K, Amagai M

Journal of Dermatological Science 23 ( 3 ) 183-190 2000.08

Research paper (scientific journal), Joint Work

Production of the entire extracellular domain of BP180(type XVII collagen)by baculovirus expression

Hata Yasuki, Fujii Yoshiko, Tsunoda Kazuyuki, Amagai Masayuki

Jounal of Dermatological Science 23 ( 3 ) 183-190 2000.08

Research paper (scientific journal), Joint Work

天疱瘡の皮膚・粘膜病変と自己抗体

Amagai Masayuki, Nishikawa Takeji

臨床免疫 34 ( 1 ) 20-25 2000.07

Research paper (scientific journal), Joint Work

Protection against pemphigus foliaceus by desmoglein 3 in neonates

Wu H, Wang ZH, Yan A, Lyle S, Fakharzadeh S, Wahl JK, Wheelock MJ, Ishikawa H, Uitto J, Amagai M, Stanley JR

New England Journal of Medicine 343 ( 1 ) 31-35 2000.07

Research paper (scientific journal), Joint Work

デスモソームと疾患

Amagai Masayuki

病理と臨床 18 ( 7 ) 691-696 2000.07

Research paper (scientific journal), Single Work

粘膜に症状が限局した尋常性天疱瘡の2症例　―抗デスモグレイン抗体価測定―

Morimotomitsuaki，Yamanegenyuki，Fukushimataihei，Morisakishigeki，Kuramotochinatsu，Oguramoto，Tanakayouichi，Takahashishinichi，Amagaimasayuki

日本口腔粘膜学会誌 6 26-31 2000.06

Research paper (scientific journal), Joint Work

Pemphigus vegetans mit Autoantikoepern gegen rekombinantes Desmoglein 3

Arnold J, Rose C, Amagai M, Brocker E, Zillikens D

Aktuelle Dermatologie 26 241-244 2000.06

Research paper (scientific journal), Joint Work

Identification of desmoglein 1 as autoantigen in a patient with intraepidermal neutrophilic type of IgA pemphigus

Karpati S, Amagai M, Li LW, Dochmowski D, Hashimoto T, Horvath A

Experimental Dermatology 9 ( 3 ) 224-228 2000.06

Research paper (scientific journal), Joint Work

皮膚の組織構築と疾患―特集に寄せて―

Amagai Masayuki, Nishikawa Takeji

組織培養工学 26 ( 6 ) 210-211 2000.06

Research paper (scientific journal), Joint Work

自己免疫性水疱症血清の蛍光抗体法ならびにELISA法による抗体検索―日米2施設で交換された各20例の未知血清の検討

Futei Yuko，Amagai Masayuki，Nishikawa Takeji

臨床皮膚科 54 ( 6 ) 393-396 2000.05

Research paper (scientific journal), Joint Work

Different effects of dominant negative mutants of desmocollin and desmoglein on the cell-cell adhesion of keratinocytes

Hanakawa Y, Amagai M, Shirakata Y, Sayama K, Hashimoto K

Journal of Cell Science 113 ( 10 ) 1803-1811 2000.05

Research paper (scientific journal), Joint Work

Development of pemphigus vulgaris in a patient with pemphigus foliaceus: antidesmoglein antibody profile shift confirmed by enzyme-linked immunosorbent assay

Ishii K, Amagai M, Ohata Y, Shimizu H, Hashimoto T, Ohya K, Nishikawa T

Journal of the American Academy of Dermatology 42 ( 5 ) 859-861 2000.05

Research paper (scientific journal), Joint Work

C-terminus of desmoyokin/AHNAK protein is responsible for its translocation between the nucleus and cytoplasm

Nie Z, Ning W, Amagai M, Green KJ, Hashimoto T

The Journal of Investigative Dermatology 114 ( 5 ) 1044-1049 2000.05

Research paper (scientific journal), Joint Work

Coexistence of psoriasis and linear IgA bullous dermatosis

Takagi Y, Sawada S, Yamauchi M, Amagai M, Niimura M

The British Journal Dermatology 142 ( 3 ) 513-516 2000.05

Research paper (scientific journal), Joint Work

Dissociation of intra- and extracellular domains of desmosomal cadherins and E-cadherin in Hailey-Hailey disease and Darier's disease

Hakuno M, Shimizu H, Akiyama M, Amagai M, Wahl JK, Wheelock MJ, Nishikawa T

The British Journal Dermatology 142 ( 2 ) 702-711 2000.04

Research paper (scientific journal), Joint Work

ELISA as a sensitive and specific diagnostic tool for pemphigus

Amagai M

Journal of National Pemphigus Foundation 22 4-13 2000.04

Research paper (scientific journal), Single Work

Use of autoantigen-knockout mice in developing an active autoimmune disease for pemphigus

Amagai Masayuki, Tsunoda Kazuyuki, Suzuki Harumi, Nishifuji Kouji, Koyasu Shigeo, Nishikawa Takeji

Journal of Clinical Investigation 105 ( 5 ) 625-631 2000.03

Research paper (scientific journal), Joint Work

抗ラミニン5型瘢痕性類天疱瘡の1例

Otsuka Tomoko, Futei Yuko, Miura Ryuji, Ohata Yoshiyuki, Amagai Masayuki, Shimizu Hiroshi, Nishikawa Takeji, Hashimoto Akihiko, Yamamoto Tatsuo

臨床皮膚科 54 ( 3 ) 209-212 2000.03

Research paper (scientific journal), Joint Work

Use of autoantigen-knockout mice in developing an active autoimmune disease model for pemphigus

Amagai Masayuki, Tsunoda Kazuyuki, Suzuki Harumi, Nishifuji Koji, Koyasu Shigeo, Nishikawa Takeji

The Journal of Clinical Investigation 105 ( 5 ) 625-631 2000.03

Research paper (scientific journal), Joint Work

Use of autoantigen knockout mice to develop an active autoimmune disease model of pemphigus

Amagai M, Tsunoda K, Suzuki H, Nishifuji K, Koyasu S, Nishikawa T

The Journal of Clinical Investigation 105 ( 5 ) 625-631 2000.03

Research paper (scientific journal), Joint Work

Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris

Proby Charlotte M, Ohta Takayuki, Suzuki Harumi, Koyasu Shigeo, Gamou Shinobu, Shimizu Nobuyoshi, Wahl James K, Wheelock Margaret J, Nishikawa Takeji, Amagai Masayuki

British Journal of Dermatology 142 ( 2 ) 1-11 2000.02

Research paper (scientific journal), Joint Work

Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris

Proby CM, Ota T, Suzuki H, Koyasu S, Gamou S, Shimizu N, Wahl JK, Wheelock MJ, Nishikawa T, Amagai M

British Journal of Dermatology 142 ( 2 ) 321-330 2000.02

Research paper (scientific journal), Joint Work

Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris

Proby CM, Ota T, Suzuki H, Koyasu S, Gamou Shinobu, Shimizu Nobuyoshi, Wahl JK, Wheelock MJ, Nishikawa T, Amagai M

The British Journal of Dermatology 142 ( 2 ) 321-330 2000.02

Research paper (scientific journal), Joint Work

Detection of antigen-specific B cells in patients with pemphigus vulgreis by enzyme-linked immunospot assay:requirement of T cell collaboretion for autoantibody production

Nishifuji Koji, Amagai Masayuki, Kuwana Masataka, Iwasaki Toshiro, Nishikawa Takeji

Journal of Investigative Dermatology 114 ( 1 ) 88-94 2000.01

Research paper (scientific journal), Joint Work

Detection of antigen-specific B cells in patients with pemphigus vulgaris by enzyme-linked immunospot (ELISPOT) assay: requirement of T cell collaboration for autoantibody production

Nishifuji K, Amagai M, Kuwana M, Iwasaki T, Nishikawa T

Journal of Investigative Dermatology 114 ( 1 ) 88-94 2000.01

Research paper (scientific journal), Joint Work

抗Dsg1 IgA抗体を有し，膿疱形成を認めた尋常性落葉状天疱瘡の1例

Futei Yuko, Ishii Ken, Hata Yasuki, Oohata Yoshiyuki, Amagai Masayuki, Shimizu Hiroshi, Nishikawa Takeji

臨床皮膚科 54 ( 1 ) 21-24 2000.01

Research paper (scientific journal), Joint Work

新しい天疱瘡の血清学的診断法

Futei Yuko, Amagai Masayuki, Nishikawa Takeji, Kinoshita Keiko, Ooya Kazuhiko

臨床皮膚科 53 ( 13 ) 1081-1086 1999.12

Research paper (scientific journal), Joint Work

Late development of anti-desmoglein 1 antibodies in pemphigus vulgaris: correlation with disease progression

Miyagawa S, Amagai M, Iida T, Muramatsu T, Yamamoto Y, Nishikawa T, Shirai T

British Journal of Dermatology 141 ( 6 ) 1084-1087 1999.12

Research paper (scientific journal), Joint Work

水疱症：抗体から細胞へ

Muramatsu Tsutomu, Amagai Masayuki

日本皮膚科学会雑誌 109 ( 12 ) 1922-1922 1999.11

Research paper (scientific journal), Joint Work

ピアスによる金皮膚炎の1例―歯科金属が憎悪因子として関与か―

Yamada Sachiko, Tanikawa Akiko, Amagai Masayuki, Nishikawa Takeji, Mutou Yuki

皮膚科の臨床 41 ( 12 ) 1925-1928 1999.11

Research paper (scientific journal), Joint Work

A case of classic pemphigus erythematosus : detection of anti-desmoglein 1 antibody by ELISA

Gomi H, Kawada A, Amagai M, Matsuo I

Dermatology 199 ( 2 ) 188-189 1999.11

Research paper (scientific journal), Joint Work

Unique immunobullous disease in a child with a predominantly IgA response to three desmosomal proteins

Gooptu C, Mendelsohn S, Amagai M, Hashimoto T, Nishikawa T, Wojnarowska E

British Journal of Dermatology 141 ( 5 ) 882-886 1999.11

Research paper (scientific journal), Joint Work

Phosphatidylcholine-specific phospholipase C, but not phospholipase D, is involved in pemphigus IgG-induced signal transduction

Seishima M, Iwasaki-Bessho Y, Itoh Y, Nozawa Y, Amagai M, Kitajima Y

Archives of Dermatological Research 291 ( 11 ) 606-613 1999.11

Research paper (scientific journal), Joint Work

天疱瘡の分子皮膚科学

Amagai Masayuki

日本皮膚科学会雑誌 109 ( 12 ) 1721-1723 1999.11

Research paper (scientific journal), Single Work

天疱瘡抗体産生B細胞の解析

Amagai Masayuki

日本皮膚科学会雑誌 109 ( 12 ) 1915-1916 1999.11

Research paper (scientific journal), Single Work

アカデミズムからの提言

Amagai Masayuki

日本皮膚科学会雑誌 109 ( 12 ) 1923-1924 1999.11

Research paper (scientific journal), Single Work

なぜ天疱瘡で水疱ができるのか？

Amagai Masayuki

皮膚病診療 21 ( 10 ) 880-884 1999.10

Research paper (scientific journal), Single Work

HLA-DRB1 polymorphisms and autoimmune responses to desmogleins in Japanese patients with pemphigus

Miyagawa S, Amagai M, Niizeki H, Yamashina Y, Kaneshige T, Nishikawa T, Shirai T, Inoko H

Tissue Antigens 54 ( 4 ) 333-340 1999.10

Research paper (scientific journal), Joint Work

A case of herpetiform pemphigus coexisting with psoriasis vulgaris

Morita E, Amagai M, Tanaka T, Horiuchi K, Mizuno H, Yamamoto S

British Journal of Dermatology 141 ( 4 ) 754-755 1999.10

Research paper (scientific journal), Joint Work

Paraneoplastic pemphigus (PNP) associated with Castleman tumor, myasthenia gravis and bronchiolitis obliterans

Chorzelski T, Hashimoto T, Maciejewska B, Amagai M, Anhalt GJ, Jablonska S

Journal of the American Academy of Dermatology 41 ( 3Pt1 ) 393-400 1999.09

Research paper (scientific journal), Joint Work

Epimorphin expression during human foetal hair follicle development

Akiyama M, Amagai M, Smith LT, Hashimoto H, Shimizu H, Nishikawa T

British Journal of Dermatology 141 ( 3 ) 447-452 1999.09

Research paper (scientific journal), Joint Work

Herpetiform pemphigus with an autoimmune response to recombinant desmoglein 1

Seitz CS, Staegemeir E, Amagai M, Rose C, Braker EB, Zillikens D

British Journal of Dermatology 141 ( 2 ) 354-355 1999.08

Research paper (scientific journal), Joint Work

Paraneoplastic pemphigus with clinical and serological features of pemphigus foliaceus

Chorzelski TP, Hashimoto T, Amagai M, Sysa-Jedrzejowska A, Choczaj A, Nousari HC, Anhalt GJ, Jablonska S

British Journal of Dermatology 141 ( 2 ) 357-359 1999.08

Research paper (scientific journal), Joint Work

Desmoglein 1 and desmoglein 3 are the target autoantigens in herpetiform pemphigus

Ishii K, Amagai M, Komai A, Ebihara T, Chorzelski TP, Jablonska S, Ohya K, Nishikawa T, Hashimoto T

Archives of Dermatology 135 ( 8 ) 943-947 1999.08

Research paper (scientific journal), Joint Work

Autoimmunity against desmosomal cadherins in pemphigus

Amagai M

Journal of Dermatological Science 20 ( 2 ) 92-102 1999.06

Research paper (scientific journal), Single Work

The new pemphigus variants

Robinson ND, Hashimoto T, Amagai M, Chan LS

Journal of the American Academy of Dermatology 40 ( 5Pt1 ) 649-671 1999.05

Research paper (scientific journal), Joint Work

The clinical phenotype of pemphigus is defined by the anti-desmoglein autoantibody profile

Amagai Masayuki, Tsunoda Kazuyuki, Detlef Zillikens, Nagai Tetsuo, Nishikawa Takej

Journal of American Academy Dermatology 40 ( 2 ) 167-170 1999.02

Research paper (scientific journal), Joint Work

Human autoantibodies against HD1/plectin in paraneoplastic pemphigus

Proby C, Fujii Y, Owaribe K, Nishikawa T, Amagai M

Journal of Investive Dermatology 112 ( 2 ) 153-156 1999.02

Research paper (scientific journal), Joint Work

Usefulness of enzyme-linked immunosorbent assay (ELISA) using recombinant desmogleins 1 and 3 for serodiagnosis of pemphigus

Amagai M, Komai A, Hashimoto T, Shirakata Y, Hashimoto T, Yamada T, Kitajima Y, Ohaya K, Iwanami H, Nishikawa T

British Journal of Dermatology 140 ( 2 ) 351-357 1999.02

Research paper (scientific journal), Joint Work

The clinical phenotype of pemphigus is defined by the anti-desmoglein autoantibody profile

Amagai M, Tsunoda K, Zillikens D, Nagai T, Nishikawa T

Journal of the American Academy of Dermatology 40 ( 2 ) 167-170 1999.02

Research paper (scientific journal), Joint Work

Desmoglein3 - ELISA: A pemphigus vulgaris-specific diagnostic tool

Lenz P, Amagai M, Volc-platzer B, Stingl G, Kirnbauer R

Archives of Dermatology 135 ( 2 ) 143-148 1999.02

Research paper (scientific journal), Joint Work

Explanation for the clinical and microscopic localization of lesions in pemphigus foliaceus and vulgaris

Mahoney Mg, Wang Z, Rothenberger Kl, Koch Pj, Amagai M, Stanley Jr

Journal of Clinical Investigation 103 ( 4 ) 461-468 1999.02

Research paper (scientific journal), Joint Work

自己免疫疾患を取り巻く最近のトピックス：天疱瘡のターゲット―デスモグレイン―

Amagai Masayuki

内科 83 ( 1 ) 119-122 1999.01

Research paper (scientific journal), Single Work

食道病変を伴った尋常性天疱瘡

Oka Hiroshi, Murata Takayuki, Saito Masataka, Kawahara Yoshie, Sasaki Yuko, Ohata Yoshiyuki, Amagai Masayuki, Nishikawa Takeji

臨床皮膚科 53 ( 1 ) 9-14 1999.01

Research paper (scientific journal), Joint Work

血清中に抗BP180抗体が認められた落葉状天疱瘡の1例

Hattori Naoko, Kasai Shinomi, Hino Haruko, Amagai Masayuki, Hashimoto Takashi

臨床皮膚科 52 ( 11 ) 1026-1029 1998.11

Research paper (scientific journal), Joint Work

結核性膿瘍の1例

Sato Tomotaka, Inazumi Toyoko, Ohata Yoshiyuki, Amagai Masayuki, Nishikawa Takeji, Aoki Takuya

臨床皮膚科 52 ( 11 ) 1030-1033 1998.11

Research paper (scientific journal), Joint Work

Preferential activation of the complement system in the lower epidermis of pemphigus vulgaris patients

Lapiere J-c, Guitart J, Ettlin Da, Chen Dm, Amagai M, Chan Ls

British Journal of Dermatology 139 ( 5 ) 851-854 1998.11

Research paper (scientific journal), Joint Work

印象記：第78回英国皮膚科学会 (BAD) に参加して

Amagai Masayuki

臨床皮膚科 52 ( 11 ) 1058-1060 1998.11

Research paper (scientific journal), Single Work

デスモゾームカドヘリン

Ishii Ken, Amagai Masayuki

医学のあゆみ 187 ( 1 ) 37-41 1998.10

Research paper (scientific journal), Joint Work

Compositionally different desmosomes in the various compartments of the human hair follicle

Kurzen H, Moll I, Moll R, Schafer S, Simics E, Amagai M, Wheelock Mj, Franke Ww

Differentiation 63 ( 5 ) 295-304 1998.09

Research paper (scientific journal), Joint Work

皮膚・粘膜症状より診断されHIV-associated eosinophilic folliculitisを伴ったAIDSの1例

Kawahara Yoshie, Kimura Yoshifumi, Oohata Yoshiyuki, Amagai Masayuki, Nishikawa Takeji, Nagai Tetsuo

臨床皮膚科 52 ( 9 ) 695-698 1998.09

Research paper (scientific journal), Joint Work

組換えバキュロウィルス抗原蛋白を用いた天疱瘡の診断と治療

Ishii Ken, Amagai Masayuki

Monthly Book Derma 14 1-6 1998.08

Research paper (scientific journal), Joint Work

Antibodies against desmoglein 3 (pemphigus vulgaris antigen) are present in sera from patients with paraneoplastic pemphigus and cause acantholysis in vivo in neonatal mice

Amagai M, Nishikawa T, Nousari Hc, Anhalt Gj, Hashimoto T

Journal of Clinical Investigation 102 ( 4 ) 775-782 1998.08

Research paper (scientific journal), Joint Work

Epidermolysis bullosa acquisita in a 6-year-old Japanese boy

Hayashi K, Ueda M, Sakai M, Fujikawa Y, Tsuru K, Amagai M, Ichihashi M

International Journal of Dermatology 37 ( 8 ) 612-614 1998.08

Research paper (scientific journal), Joint Work

皮膚・粘膜症状より診断されHIV-associated eosinophilic folliculitisを伴ったAIDSの1例

Kawahara Yoshie, Kimura Yoshifumi, Ohata Yoshiyuki, Amagai Masayuki, Nishikawa Takeji, Nagai Tetsuo

臨床皮膚科 52 ( 9 ) 695-698 1998.07

Research paper (scientific journal), Joint Work

スナノミ症(tungiasis)の2例

Oka Hiroshi, Tanikawa Akiko, Sato Tomotaka, Sasaki Yuko, Amagai Masayuki, Kobayashi Seiki, Takeuchi Tsutomu, Shinonaga Satoshi

臨床皮膚科 52 ( 8 ) 605-607 1998.07

Research paper (scientific journal), Joint Work

疱疹状天疱瘡の1例

Saito Hitoshi, Futei Yuko, Konohana Izumi, Ishii Ken, Kawahara Yoshie, Amagai Masayuki

臨床皮膚科 52 ( 7 ) 518-521 1998.06

Research paper (scientific journal), Joint Work

Novel non-radioisotope immunoprecipitation studies indicate involvement of pemphigus vulgaris antigen in paraneoplastic pemphigus

Hashimoto T, Amagai M, Ning W, Nishikawa T, Karashima T, Mori O, Jablonska S, Chorzelski Tp

Journal of Dermatological Science 17 ( 2 ) 132-139 1998.06

Research paper (scientific journal), Joint Work

天疱瘡・類天疱瘡

Amagai Masayuki

最新医学　自己免疫疾患の臨床1998（II）―疾患概念と最新の知見 ― 53 1613-1621 1998.06

Research paper (scientific journal), Single Work

A case of cicatricial pemphigoid with simultaneous IgG autoantibodies against the 180 kD bullous pemphigoid antigen and laminin-5

Kawahara Y, Amagai M, Ohata Y, Ishii K, Hasegawa Y, Hsu R, Yee C, Yancey Kb, Nishikawa T

Journal of American Academy of Dermatology 38 ( 4 ) 624-627 1998.04

Research paper (scientific journal), Joint Work

Heterogeneous MHC II restriction pattern of autoreactive desmoglein 3 specific T cell responses in pemphigus vulgaris patients and normals

Hertl M, Karr Rw, Amagai M, Katz Si

Journal of Investigative Dermatology 110 ( 4 ) 388-392 1998.04

Research paper (scientific journal), Joint Work

後天性表皮水疱症の1例

Fujita Tomoko, Orihara Midori, Kokaji Kazuko, Hayakawa Kazuto, Shiohara Tetsuo, Kawahara Yoshie, Amaya Masayuki

皮膚科の臨床 40 ( 2 ) 275-279 1998.02

Research paper (scientific journal), Joint Work

Lack of mucosal involvement in pemphigus foliaceus may be due to low expression of desmoglein 1

Shirakata Y, Amagai M, Hanakawa Y, Nishikawa T, Hashimoto K

J Invest Dermatol 110 ( 1 ) 76-78 1998.01

Research paper (scientific journal), Joint Work

Recognition of desmoglein 3 by autoreactive T cells in pemphigus vulgaris patients and normals

Hertl M, Amagai M, Sundaram H, Stanley J, Ishii K, Katz SI

J Invest Dermatol 110 ( 1 ) 62-66 1998.01

Research paper (scientific journal), Joint Work

Desmoglein 3 (DSG3)

Amagai Masayuki

Molecular Medicine 34 ( 増刊 ) 136-137 1997.12

Research paper (scientific journal), Single Work

分子生物学：天疱瘡病態解明ストラテジー

Amagai Masayuki

日本皮膚科学会雑誌 107 ( 13 ) 1613-1614 1997.12

Research paper (scientific journal), Single Work

カドヘリンの異常と天疱瘡

Amagai Masayuki, Ishii Ken, Nishikawa Takeji

Molecular Medicine 34 ( 10 ) 1202-1209 1997.10

Research paper (scientific journal), Joint Work

皮膚疾患と接着分子

Amagai Masayuki

Bio Clinica 12 ( 3 ) 957-960 1997.10

Research paper (scientific journal), Single Work

印象記：第19回世界皮膚科学会に参加して

Amagai Masayuki

臨床皮膚科 51 ( 10 ) 874-876 1997.09

Research paper (scientific journal), Single Work

Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins

Ishii K, Amagai M, Hall RP, Hashimoto T, Takayanagi A, Gamou S, Shimizu N, Nishikawa T

J Immunol 159 ( 4 ) 2010-2017 1997.08

Research paper (scientific journal), Joint Work

Human desmocollin 1 (Dsc1) is an autoantigen for the subcorneal pustular dermatosis type of IgA pemphigus

Hashimoto T, Kiyokawa C, Mori O, Miyasato M, Chidgey MAJ, Garrod DR, Kobayashi Y, Komori K, Ishii K, Amagai M, Nishikawa T

J Invest Dermatol 109 ( 2 ) 127-131 1997.08

Research paper (scientific journal), Joint Work

Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3)

Kubo A, Amagai M, Hashimoto T, Doi T, Higashiyama M, Hashimoto K, Yoshikawa K

Br J Dermatol 137 109-113 1997.07

Research paper (scientific journal), Joint Work

表皮ケラチノサイトの細胞接着分子と膜裏打ち蛋白(3)―細胞内情報伝達機構における役割と分化・増殖・癌化との関連―

Hashimoto Takashi, Amagai Masayuki

西日本皮膚科 59 ( 3 ) 402-406 1997.06

Research paper (scientific journal), Joint Work

Pemphigus foliaceus : Preferential binding of IgG1 and C3 at the upper epidermis

Hernandez C, Amagai M, Woodley DT, Chan LS

Br J Dermatol 136 249-252 1997.04

Research paper (scientific journal), Joint Work

Hypomelanosis of Itoの1例

Amagai Masayuki, Harada Reiko, Muroya Koji, Ogata Tsutomu, Akiyama Masashi

臨床皮膚科 51 ( 4 ) 309-312 1997.04

Research paper (scientific journal), Joint Work

表皮ケラチノサイトの細胞接着分子と膜裏打ち蛋白(2)―細胞接着における役割とその制御―

Hashimoto Takashi, Amagai Masayuki

西日本皮膚科 59 ( 2 ) 257-265 1997.04

Research paper (scientific journal), Joint Work

表皮ケラチノサイトの細胞接着分子と膜裏打ち蛋白（1）--細胞接着構造と構成蛋白--

Hashimoto Takashi, Amagai Masayuki

西日本皮膚科 59 ( 1 ) 87-93 1997.02

Research paper (scientific journal), Joint Work

Pemphigus foliaceus: preferential binding of IgG1 and C3 at the upper epidermis

Hernandez C, Amagai M, Chan LS

Br J Dermatol 136 ( 2 ) 249-252 1997.02

Research paper (scientific journal), Joint Work

カドヘリンの自己免疫病としての皮膚疾患

Amagai Masayuki

第107回日本医学会シンポジウム記録集 45-51 1996.11

Research paper (scientific journal), Single Work

Analysis of antigens recognized by autoantibodies in herpes gestationis: Usefulness of immunoblotting using a fusion protein representing an extracellular domain of the 180 kD bullous pemphigoid antigen

Murakami H, Amagai M, Higashiyama M, Hashimoto K, Chorzelski TP, Bhogal BS, Black MM, Zillikens D, Nishikawa T, Hashimoto T

J Dermatol Sci 13 ( 2 ) 112-117 1996.11

Research paper (scientific journal), Joint Work

Transport to endoplasmic reticulum by signal peptide, but not proteolytic processing, is inquired for formation of conformational epitopes of pemphigus vulgaris antigen (Dsg3)

Amagai Masayuki, Ishii Ken, Takayanagi Atsushi, Nishikawa Takeji, Shimizu Nobuyoshi

J Invest Dermatol 107 ( 4 ) 539-542 1996.10

Research paper (scientific journal), Joint Work

The majority of bullous pemphigoid and herpes gestationis serum samples react with NC16a domain of the 180 kD bullous pemphigoid antigen

Matsumura K, Hashimoto T, Amagai M, Nishikawa T

Arch Dermatol Res 288 ( 9 ) 507-509 1996.08

Research paper (scientific journal), Joint Work

Clinical evaluation of enzyme-linked immunosorbent assay (ELISA) for pemphigus using baculovirus-expressed antigen

Ishii Ken, Amagai Masayuki, Hashimoto Takashi, Hall R P, Takayanagi Atsushi, Gamou Shinobu, Shimizu Nobuyoshi, Nishikawa Takeji

J Dermatol Sci 12 ( 2 ) 191-191 1996.06

Research paper (scientific journal), Joint Work

Transport to endoplasmic reticulum (ER) by signal peptide, but not proteolytic processing, is required for formation of conformational epitopes of pemphigus vulgaris antigen (Dsg3)

Amagai Masayuki, Ishii Ken, Takayanagi Atsushi, Nishikawa Takeji, Shimizu Nobuyoshi

J Dermatol Sci 12 ( 2 ) 191-191 1996.06

Research paper (scientific journal), Joint Work

Specific detection of anti-cell surface antibodies in herpes gestationis sera

Hashimoto T, Amagai M, Murakami H, Higashiyama M, Hashimoto K, Nishikawa T

Exp Dermatol 5 ( 2 ) 96-101 1996.04

Research paper (scientific journal), Joint Work

Pemphigus: from immunofluorescence to molecular biology

Nishikawa T, Hashimoto T, Shimizu H, Ebihara T, Amagai M

J Dermatol Sci 12 ( 1 ) 1-9 1996.04

Research paper (scientific journal), Joint Work

Immunofluorescence and immunoblot studies on the reactivity of pemphigus vulgaris and pemphigus foliaceus sera with desmoglein 3 and desmoglein 1

Hashimoto T, Amagai M, Garrod DR

Epith Cell Biol 4 ( 2 ) 63-69 1996.04

Research paper (scientific journal), Joint Work

A case of herpetiform pemphigus associated with autoimmune hemolytic anemiai detection of autoantibodies against multiple epidermal antigens

Shimizu K, Hashimoto T, Wang N, Watanabe K, Ohata Y, Kikuchi A, Amagai M, Nishikawa T

Dermatology 192 ( 2 ) 179-182 1996.03

Research paper (scientific journal), Joint Work

Pemphigus vulgaris antigen (Desmoglein 3) is localized in the lower epidermis, the site of blister formation in patients

Amagai M, Koch PJ, Nishikawa T, Stanley JR

J Invest Dermatol 106 ( 2 ) 351-355 1996.02

Research paper (scientific journal), Joint Work

Lymphomatoid papulosis followed by Ki-1 positive anaplastic large cell lymphoma ・ Proliferation of a common T-cell clone

Amagai M, Kawakubo Y, Tsuyuki A, Harada R

J Dermatol 22 ( 10 ) 743-746 1995.10

Research paper (scientific journal), Joint Work

Conformational epitopes of pemphigus antigens (Dsg1 and Dsg3) are Calcium dependent and glycosylation independent

Amagai M, Ishii K, Hashimoto T, Gamou S, Shimizu N, Nishikawa T

J Invest Dermatol 105 ( 2 ) 243-247 1995.08

Research paper (scientific journal), Joint Work

Localization of the human AHNAK/desmoyokin gene (AHNAK) to chromosome band 11q 12 by somatic cell hybrid analysis and fluorescence in situ hybridizati

Kudoh J, Wang N, Minoshima S, Hashimoto T, Amagai M, Nishikawa T, Shtivelman E, Bishop J, Shimizu N

Cytogenet Cell Genet 70 ( 3ー4 ) 218-220 1995.07

Research paper (scientific journal), Joint Work

Antigen-specific immunoadsorption of pathogenic autoantibodies in pemphigus foliaceus

Amagai M, Hashimoto T, Green KJ, Shimizu N, Nishikawa T

J Invest Dermatol 104 ( 6 ) 895-901 1995.06

Research paper (scientific journal), Joint Work

Characterization of paraneoplastic pemphigus autoantigens by immunoblot analysis

Hashimoto T, Amagai M, Watanabe K, Chorzelski T, Bhogal B, Black M, Stevens H, Boorsma D, Korman N, Gamou S, Shimizu N, Nishikawa T

J Invest Dermatol 104 ( 5 ) 829-834 1995.05

Research paper (scientific journal), Joint Work

A case of pemphigus vulgaris showing reactivity with pemphigus antigens (Dsg1 and Dsg3) and desmocollins

Hashimoto T, Amagai M, Watanabe K, Dmochowski M, Chidgey M, Yue K, Garrod D, Nishikawa T

J Invest Dermatol 104 ( 4 ) 541-544 1995.04

Research paper (scientific journal), Joint Work

Detection of autoantibodies against bullous pemphigoid and pemphigus antigens by an enzyme-linked immunosorbent assay using the bacterial recombinant

Ide A, Hashimoto T, Amagai M, Tanaka M, Nishikawa T

Exp Dermatol 4 ( 2 ) 112-116 1995.04

Research paper (scientific journal), Joint Work

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