論文 - 貴志　和生

Transplanted endothelial progenitor cells augment the survival areas of rat dorsal flaps

Kubota Yoshiaki, Kishi Kazuo, Satoh Hiroko, Tanaka Takara, Nakajima Hideo, Nakajima Tatsuo

Cell Transplantation 12 （ 6 ） 647 - 657 2003年

ISSN  0963-6897

　概要を見る

<p>Endothelial progenitor cells (EPCs) have been identified in peripheral blood, and have been reported to be incorporated into ischemic regions such as the ischemic hindlimb. In this study, we examined whether or not transplantation of EPCs is useful for salvaging surgical flaps in vivo. At the same time, we quantitatively compared the neovascularization ability of transplanted EPCs and that of mature endothelial cells (ECs). ECs obtained from the aorta of rats by explantation and passaged several times were used in the present study. EPCs were obtained from the blood of rat hearts. The blood samples were separated by density gradient centrifugation. Light-density mononuclear cells (MNCs) were collected and cultured on plastic plates coated with rat plasma vitronectin. Cells attached at day 7 of culture were deemed to be EPCs. Then PBS (control), ECs, or EPCs (3.0 × 10⁵ suspended in 1.0 ml PBS) were injected at the middle of a flap. Seven days after surgery, the survival lengths of the flaps were evaluated. EPC-transplanted groups revealed statistically significant augmentation of survival length compared with the other two groups (p &lt; 0.003). EPC-transplanted groups had significantly more angiographically detectable blood vessels (p &lt; 0.003) and significantly higher capillary density (p &lt; 0.03) than the other two groups. Confocal microscopy revealed that EPCs were incorporated into enhanced neovascularization. These results suggest that transplantation of EPCs may be useful for salvaging surgical flaps, and EPCs are superior to ECs in neovascularization ability.</p>

A challenge to construct functional tissue engineered skeletal muscle

Kishi Kazuo, Satoh Hiroko, Tanaka Takara, Kubota Yoshiaki, Imanishi Nobuaki, Nakajima Hideo, Nakajima Tatsuo

Japanese Journal of Plastic and Reconstructive Surgery 45 （ 5 ） 419 - 424 2002年

ISSN  0021-5228

　概要を見る

<p>In order to construct functional skeletal muscle in vitro for tissue engineering, the micro architecture including microcirculation, neuromuscular junctions, and cell alignment has to be constructed. Thus it has been said that tissue engineered skeletal muscle will not be realized until two or three decades in the future. Skeletal muscle, however, possesses the ability to regenerate partial defects even in adult animals, and this regenerative activity can be a blue print for skeletal muscle tissue engineering. In the present paper, we describe the principle of skeletal muscle regeneration, and then describe the challenges to achieve skeletal muscle tissue engineering.</p>

骨格筋のTissue Engineering のチャレンジと展望

貴志和生，佐藤博子，久保田義顕，中島龍夫

形成外科 45 （ 5 ） 419-424 2002年

研究論文（学術雑誌）, 共著

胎仔の創傷治癒―胎仔型から成獣型創傷治癒への変換点を探る

貴志和生

医学のあゆみ 198 （ 11 ） 776-777 2001年09月

研究論文（学術雑誌）, 単著

Fetal scarless wound healing

Kishi K.

Connective Tissue 32 （ 4 ） 361 - 366 2000年

ISSN  0916-572X

　概要を見る

<p>Fetal cutaneous wound made at a proper developmental stage leaves any scar formation and regenerates normal skin completely. Thus fetal wound healing can be a model of regeneration in mammalian. In this review, various differences between adult and fetal wound healing from molecular, cellular, and morphological aspects are introduced to understand the mechanism of regeneration. Then the transition from fetal to adult type wound healing is discussed in detail.</p>

Differential responses of collagen and glycosaminoglycan syntheses and cell proliferation to exogenous transforming growth factor β1 in the developing mouse skin fibroblasts in culture

Kishi Kazuo, Nakajima Hideo, Tajima Shingo

British Journal of Plastic Surgery 52 （ 7 ） 579 - 582 1999年10月

ISSN  0007-1226

　概要を見る

<p>Skin fibroblast cultures were established from mouse foetuses at days 14, 15, 16 and 18 of gestation and from newborn mice. Modulations of mitotic and biosynthetic phenotypes of the fibroblasts by transforming growth factor β1 (TGFβ1) were studied. Treatment of the fibroblasts with TGFβ1 at doses of 1 and 10 ng/ml for 48 h resulted in significant stimulation of cell proliferation in the 15-, 16- and 18-day foetal fibroblasts and a slight stimulation in the 14-day foetal fibroblasts. Treatment with TGFβ1 resulted in stimulation of collagen synthesis approximately 2-fold in the 18-day foetal and newborn fibroblasts, but failed to stimulate it in the 14-, 15- and 16-day foetal fibroblasts. TGFβ1 stimulated glycosaminoglycan (GAG) synthesis throughout all developmental stages approximately 1.8-2.6 fold. Histological study demonstrated that skin wounds made at day 16 of gestation were replaced with normal-appearing dermis, but at day 18 the wounds left dermal fibrosis and lack of hair follicles. These results indicate that the modulations of fibroblast phenotypes (proliferation and syntheses of collagen and GAG) in response to TGFβ1 occur at different stages of gestation. Ontogenic transitions of skin wound healing and collagen synthetic phenotype with TGFβ1 treatment in cultured fibroblasts occurred between days 16 and 18 of gestation, suggesting that the unresponsiveness of collagen synthesis to exogenous TGFβ1 in cell culture may be related to the phenomenon of scarless wounds in the foetus.</p>

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骨浸潤，頸部リンパ節転移を認めた後頭部有棘細胞癌の1例

高橋慎一，川島淳子，貴志和生，中嶋英雄

日本皮膚外科学会誌 1 （ 1 ） 92-92 1998年02月

研究論文（学術雑誌）, 共著

磁場が線維芽細胞TGF-β₁ 発現に及ぼす影響

貴志和生，土屋裕一，中嶋英雄，多島新吾

日本生体磁気学会誌特別号 9 （ 1 ） 68-69 1996年05月

研究論文（学術雑誌）, 共著

磁場が線維芽細胞コラーゲン合成に及ぼす影響

貴志和生，土屋裕一，中嶋英雄，多島新吾

日本生体磁気学会誌特別号 9 （ 1 ） 66-67 1996年05月

研究論文（学術雑誌）, 共著

肋間動脈穿通枝によって栄養される肋骨附き広背筋皮弁による下顎再建の経験

平野早秀子，金子剛，貴志和生，小林正弘，藤野豊美，藤井正人，大野芳裕，神崎仁，柴秀行，河奈裕正，朝波惣一郎

頭頚部腫瘍 22 （ 1 ） 120-126 1996年03月

研究論文（学術雑誌）, 共著

定常磁場曝露による皮弁生着に及ぼす影響

福積聡，中嶋英雄，今西宣晶，貴志和生，土屋裕一，大西裕子

日本生体磁気学会誌 8 （ 1 ） 168-171 1995年05月

研究論文（学術雑誌）, 共著

皮弁虚血再灌流による活性酸素産生に対する磁場の影響

土屋裕一，中嶋英雄，福積聡，今西宣晶，貴志和生，大西裕子

日本生体磁気学会誌 8 （ 1 ） 164-167 1995年05月

研究論文（学術雑誌）, 共著