Details of a Researcher - Kano, Motohiro

Papers - Kano, Motohiro

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Comparison of Postoperative Respiratory Function Between Patients After Thoracoscopic and Open Lobectomy

Ishimaru T., Kanamori Y., Fujino A., Yoneda A., Fujiogi M., Yamamoto Y., Kano M., Koinuma G., Deie K., Kawashima H.

Journal of Laparoendoscopic and Advanced Surgical Techniques 34 ( 4 ) 376 - 379 2024.04

ISSN 10926429

Purpose: This study aimed to compare respiratory functions of patients after thoracoscopic lobectomy (TS) with those after thoracotomy (TR). Methods: This retrospective study was conducted in two centers, one of which adapted TS as a standard procedure in 2009 and the other performs it via TR. Data on patients who underwent lobectomy for congenital lung disease between 2009 and 2021 and underwent pulmonary function test (spirometry) were collected. Results: Ten patients underwent TS and 36 underwent TR. Distribution based on sex, prenatal diagnosis, pathological diagnosis, and resected lobe were similar between the two groups. The median [interquartile range] age at procedure in the TR group was significantly smaller than that in the TS group (13 [11-18] months versus 38 [13-79] months, P = .03). The procedure duration in the TR group was significantly shorter than that in the TS group (230 [171-264] minutes versus 264 [226-420] minutes, P = .02). Pulmonary function test was conducted at the age of eight in both groups, but the interval between the procedure, and the test was significantly shorter in the TS group (TR: 7 [5-8] years versus TS: 5 [2-7] years, P = .03). The ratio of forced vital capacity compared to predicted one (TR: 86.6 [76.6-95.3] versus TS: 88.7 [86.8-89.1], P = .58) and the ratio of forced expiratory volume in 1 second against that predicted (TR: 84.0 [80.5-88.7] versus TS: 88.7 [86.8-89.1], P = .08) were not significantly different between the two groups. Conclusions: Although TR was performed earlier than TS, respiratory function was similar between the two groups.

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Long-Term Outcomes of Infantile Sacrococcygeal Teratoma: Results from a Multi-Institutional Retrospective Observational Study in Japan

Fumino S., Hirohata Y., Takayama S., Tajiri T., Usui N., Taguchi T., Kano M., Tomita H., Sasaki T., Inoue M., Yamoto S., Hatata T., Nakagawa Y., Endo N., Touma M., Nakata M., Tamura R., Kawakubo N., Shinkai T., Yoshida M., Tanaka S., Kurobe M., Watanabe R., Fukuhara M., Tsuji Y., Kato Y., Arai Y.

Journal of Pediatric Surgery 59 ( 4 ) 587 - 592 2024.04

ISSN 00223468

Background: Tumor recurrence, anorectal and urinary dysfunction, and lower limb dysfunction after surgery are observed in infantile sacrococcygeal teratoma (SCT). In this paper, a multi-institutional retrospective observational study was conducted to clarify the long-term functional prognosis in Japan. Methods: This study was conducted using a paper-based questionnaire distributed to 192 facilities accredited by the Japanese Society of Pediatric Surgeons, covering patients who underwent radical surgery at less than 1 year old and who survived for at least 180 days after birth from 2000 to 2019. Results: A total of 355 patients were included in this analysis. Altman type was I-II in 248 and type III-IV in 107, and the median maximum tumor diameter was 6.1 (range: 0.6–36.0) cm. There were 269 mature teratomas, 69 immature teratomas, and 10 malignant tumors. Total resection was performed in 325, subtotal or partial resection in 27, and surgical complications were noted in 54. The median postoperative follow-up was 6.6 (0.5–21.7) years. Eighty-three patients (23.4 %) had functional sequelae, including 62 (17.5 %) with anorectal dysfunction, 56 (13.0 %) with urinary dysfunction, and 15 (4.2 %) with lower limb motor dysfunction. Recurrence occurred in 42 (11.8 %) at a median age of 16.8 (1.7–145.1) months old. Risk factors for dysfunction included preterm delivery, a large tumor diameter, Altman type III-IV, incomplete resection, and surgical complications. Risk factors for recurrence included immature teratoma or malignancy, incomplete resection, and surgical complications. Conclusions: Postoperative dysfunction was not low at 23.4 %, and 11.8 % of the patients experienced recurrence occurring more than 10 years after surgery, suggesting the need for periodic imaging and tumor markers evaluations in patients with risk factors. It is necessary to establish treatment guidelines for best practice monitoring of the long-term quality of life. Level of evidence: Level II Retrospective Study.

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Evaluation of safety and efficacy of autologous oral mucosa-derived epithelial cell sheet transplantation for prevention of anastomotic restenosis in congenital esophageal atresia and congenital esophageal stenosis

Fujino A., Fuchimoto Y., Mori T., Kano M., Yamada Y., Ohno M., Baba Y., Isogawa N., Arai K., Yoshioka T., Abe M., Kanai N., Takagi R., Maeda M., Umezawa A.

Stem Cell Research and Therapy 14 ( 1 ) 2023.12

Background: We performed the first autologous oral mucosa-derived epithelial cell sheet transplantation therapy in a patient with refractory postoperative anastomotic stricture in congenital esophageal atresia (CEA) and confirmed its safety. In this study, patients with CEA and congenital esophageal stenosis were newly added as subjects to further evaluate the safety and efficacy of cell sheet transplantation therapy. Methods: Epithelial cell sheets were prepared from the oral mucosa of the subjects and transplanted into esophageal tears created by endoscopic balloon dilatation (EBD). The safety of the cell sheets was confirmed by quality control testing, and the safety of the transplantation treatment was confirmed by 48-week follow-up examinations. Results: Subject 1 had a stenosis resected because the frequency of EBD did not decrease after the second transplantation. Histopathological examination of the resected stenosis revealed marked thickening of the submucosal layer. Subjects 2 and 3 did not require EBD for 48 weeks after transplantation, during which time they were able to maintain a normal diet by mouth. Conclusions: Subjects 2 and 3 were free of EBD for a long period of time after transplantation, confirming that cell sheet transplantation therapy is clearly effective in some cases. In the future, it is necessary to study more cases; develop new technologies such as an objective index to evaluate the efficacy of cell sheet transplantation therapy and a device to achieve more accurate transplantation; identify cases in which the current therapy is effective; and find the optimal timing of transplantation; and clarify the mechanism by which the current therapy improves stenosis. Trial registration: UMIN, UMIN000034566, registered 19 October 2018, https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000039393.

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Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene

Vansandt L.M., Meinsohn M.C., Godin P., Nagykery N., Sicher N., Kano M., Kashiwagi A., Chauvin M., Saatcioglu H.D., Barnes J.L., Miller A.G., Thompson A.K., Bateman H.L., Donelan E.M., González R., Newsom J., Gao G., Donahoe P.K., Wang D., Swanson W.F., Pépin D.

Nature Communications 14 ( 1 ) 2023.12

Eighty percent of the estimated 600 million domestic cats in the world are free-roaming. These cats typically experience suboptimal welfare and inflict high levels of predation on wildlife. Additionally, euthanasia of healthy animals in overpopulated shelters raises ethical considerations. While surgical sterilization is the mainstay of pet population control, there is a need for efficient, safe, and cost-effective permanent contraception alternatives. Herein, we report evidence that a single intramuscular treatment with an adeno-associated viral vector delivering an anti-Müllerian hormone transgene produces long-term contraception in the domestic cat. Treated females are followed for over two years, during which transgene expression, anti-transgene antibodies, and reproductive hormones are monitored. Mating behavior and reproductive success are measured during two mating studies. Here we show that ectopic expression of anti-Müllerian hormone does not impair sex steroids nor estrous cycling, but prevents breeding-induced ovulation, resulting in safe and durable contraception in the female domestic cat.

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Historical Cohort Study of Congenital Isolated Hypoganglionosis of the Intestine: Determining the Best Surgical Interventions

Yamada Y., Mori T., Takahashi N., Fujimura T., Kano M., Kato M., Takahashi M., Shimojima N., Watanabe T., Yoshioka T., Kanamori Y., Kuroda T., Fujino A.

Biomolecules 13 ( 10 ) 2023.10

No standard diagnostic method or surgical treatment for congenital isolated hypoganglionosis (CIHG) has been established. This study aimed to analyze the clinical outcomes of patients with CIHG and identify the best surgical interventions provided thus far. Data on surgical interventions in 19 patients were collected between 1992 and 2020, including the type of enterostomy, type of revision, and length of the intestines. Ganglion cells in the myenteric plexus were enumerated using Hu C/D staining. The ratio of the length of the small intestine to its height was defined as the intestinal ratio (IR). The outcomes were assessed using the stoma output, growth parameters including the body mass index (BMI), and parenteral nutrition (PN) dependency. All patients required a diverting enterostomy. The IR ranged from 0.51 to 1.75 after multiple non-transplant surgeries. The stoma types were tube-stoma, end-stoma, Santulli-type, and Bishop–Koop (BK)-type. Patients with Santulli- or BK-type stomas had better BMIs and less PN dependency in terms of volume than those with end-stomas or tube-stomas. Two patients with BK-type stomas were off PN, and three who underwent an intestinal transplantation (Itx) achieved enteral autonomy. The management of CIHG involves a precise diagnosis using Hu C/D staining, neonatal enterostomy, and stoma revision using the adjusted IR and Itx if other treatments do not enable enteral autonomy.

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Recurrent outflow obstruction of a choked catheter for peritoneal dialysis caused by a fallopian tube fimbria in a five-month-old female

Furugane R., Kobayashi T., Hashizume N., Mori T., Watanabe E., Kano M., Takahashi M., Murakoshi M., Sato M., Kamei K., Fujino A., Yoneda A., Kanamori Y.

Journal of Pediatric Surgery Case Reports 76 2022.01

Outflow obstruction of a catheter for peritoneal dialysis (PD) is one of the most common complications in patients who are undergoing PD for renal failure. Although the cause of catheter obstruction is variable, a choked catheter caused by a fallopian tube fimbria is quite rare and only five pediatric cases were reported. We report a case of a five-month-old female who suffered from recurrent catheter obstruction by a fallopian tube fimbria. Laparoscopic surgery was performed to recanalize the catheter, but the catheter obstruction recurred three days after the surgery. We abandoned the use of the catheter that had been inserted into the Douglas fossa and a new catheter was inserted into the left para-colonic groove. After the second surgery, catheter obstruction did not occur and PD has been continued without any complications.

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“Triple duodenal obstruction” complicated with intestinal malrotation and several cardiovascular malformations

Takahashi M., Kanamori Y., Kobayashi T., Kobayashi M., Tsukamoto K., Yamagishi S., Furugane R., Kutsukake M., Mori T., Kano M., Yoneda A., Ito Y., Fujino A.

Journal of Pediatric Surgery Case Reports 75 2021.12

Triple duodenal obstruction is an extremely rare disorder—apparently only two cases were reported in the English literature. We present a third case complicated with some cardiovascular anomalies (interrupted inferior vena cava, left-sided inferior vena cava, preduodenal portal vein, and ventricular septal defect) and intestinal malrotation. Operative procedure to reconstruct continuity of the duodenal lumen was a combination of longitudinal incision, partial web resection, and transverse closure at the first obstruction site together with diamond-shaped duodeno-duodenostomy between the oral side of the second obstruction and the anal side of the third obstruction.

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H-type ano-bulbar urethral fistula with an ectopic perineal fistula in a male patient complicated with VACTER association

Watanabe E., Hashizume N., Furugane R., Kobayashi T., Mori T., Kano M., Takahashi M., Yamazaki T., Hasegwa Y., Fujino A., Yoneda A., Kanamori Y.

Journal of Pediatric Surgery Case Reports 75 2021.12

Congenital H-type fistula is a rare congenital rectourogenital anomaly and is less common in males than in females. A one-year-and-eight-month-old boy with an ectopic perineal fistula was incidentally diagnosed with H-type fistula when he underwent voiding cystourethrography for vesicoureteral reflux. Further examination revealed that the H-type fistula was an ano-bulbar urethral fistula. At two years of age, the patient underwent fistulectomy and also anterior sagittal anorectoplasty for the ectopic perineal fistula during the same surgery.

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Congenital dermal sinus in the lateral buttock

Kobayashi T., Kanamori Y., Furugane R., Mori T., Kano M., Takahashi M., Fujino A.

Journal of Pediatric Surgery Case Reports 75 2021.12

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Nodular fasciitis occurring at the anterior abdominal wall in a 12-year-old female

Kobayashi T., Kanamori Y., Kutsukake M., Takahashi M., Kano M., Mori T., Yamagishi S., Furugane R., Fujino A., Shiobara T., Tsutsumi Y., Miyazaki O., Haga C., Yoshioka T.

Journal of Pediatric Surgery Case Reports 74 2021.11

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Single-cell sequencing reveals suppressive transcriptional programs regulated by MIS/AMH in neonatal ovaries

Meinsohn M.C., Saatcioglu H.D., Wei L., Li Y., Horn H., Chauvin M., Kano M., Nguyen N.M.P., Nagykery N., Kashiwagi A., Samore W.R., Wang D., Oliva E., Gao G., Morris M.E., Donahoe P.K., Pépin D.

Proceedings of the National Academy of Sciences of the United States of America 118 ( 20 ) 2021.05

ISSN 00278424

Müllerian inhibiting substance (MIS/AMH), produced by granulosa cells of growing follicles, is an important regulator of folliculogenesis and follicle development. Treatment with exogenous MIS in mice suppresses follicle development and prevents ovulation. To investigate the mechanisms by which MIS inhibits follicle development, we performed single-cell RNA sequencing of whole neonatal ovaries treated with MIS at birth and analyzed at postnatal day 6, coinciding with the first wave of follicle growth. We identified distinct transcriptional signatures associated with MIS responses in the ovarian cell types. MIS treatment inhibited proliferation in granulosa, surface epithelial, and stromal cell types of the ovary and elicited a unique signature of quiescence in granulosa cells. In addition to decreasing the number of growing preantral follicles, we found that MIS treatment uncoupled the maturation of germ cells and granulosa cells. In conclusion, MIS suppressed neonatal follicle development by inhibiting proliferation, imposing a quiescent cell state, and preventing granulosa cell differentiation.

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