Tani, Hidenori



School of Medicine, Joint Research Laboratory for Medical Innovation in Heart Disease (Shinanomachi)


Project Assistant Professor (Non-tenured)/Project Research Associate (Non-tenured)/Project Instructor (Non-tenured)


Papers 【 Display / hide

  • Production of functional cardiomyocytes and cardiac tissue from human induced pluripotent stem cells for regenerative therapy

    Tani H., Tohyama S., Kishino Y., Kanazawa H., Fukuda K.

    Journal of Molecular and Cellular Cardiology (Journal of Molecular and Cellular Cardiology)  164   83 - 91 2022.03

    ISSN  00222828

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    The emergence of human induced pluripotent stem cells (hiPSCs) has revealed the potential for curing end-stage heart failure. Indeed, transplantation of hiPSC-derived cardiomyocytes (hiPSC-CMs) may have applications as a replacement for heart transplantation and conventional regenerative therapies. However, there are several challenges that still must be overcome for clinical applications, including large-scale production of hiPSCs and hiPSC-CMs, elimination of residual hiPSCs, purification of hiPSC-CMs, maturation of hiPSC-CMs, efficient engraftment of transplanted hiPSC-CMs, development of an injection device, and avoidance of post-transplant arrhythmia and immunological rejection. Thus, we developed several technologies based on understanding of the metabolic profiles of hiPSCs and hiPSC derivatives. In this review, we outline how to overcome these hurdles to realize the transplantation of hiPSC-CMs in patients with heart failure and introduce cutting-edge findings and perspectives for future regenerative therapy.

  • Intramyocardial Transplantation of Human iPS Cell–Derived Cardiac Spheroids Improves Cardiac Function in Heart Failure Animals

    Kawaguchi S., Soma Y., Nakajima K., Kanazawa H., Tohyama S., Tabei R., Hirano A., Handa N., Yamada Y., Okuda S., Hishikawa S., Teratani T., Kunita S., Kishino Y., Okada M., Tanosaki S., Someya S., Morita Y., Tani H., Kawai Y., Yamazaki M., Ito A., Shibata R., Murohara T., Tabata Y., Kobayashi E., Shimizu H., Fukuda K., Fujita J.

    JACC: Basic to Translational Science (JACC: Basic to Translational Science)  6 ( 3 ) 239 - 254 2021.03

    ISSN  2452302X

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    The severe shortage of donor hearts hampered the cardiac transplantation to patients with advanced heart failure. Therefore, cardiac regenerative therapies are eagerly awaited as a substitution. Human induced pluripotent stem cells (hiPSCs) are realistic cell source for regenerative cardiomyocytes. The hiPSC-derived cardiomyocytes are highly expected to help the recovery of heart. Avoidance of teratoma formation and large-scale culture of cardiomyocytes are definitely necessary for clinical setting. The combination of pure cardiac spheroids and gelatin hydrogel succeeded to recover reduced ejection fraction. The feasible transplantation strategy including transplantation device for regenerative cardiomyocytes are established in this study.

  • Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells

    Someya S., Tohyama S., Kameda K., Tanosaki S., Morita Y., Sasaki K., Kang M.I., Kishino Y., Okada M., Tani H., Soma Y., Nakajima K., Umei T., Sekine O., Moriwaki T., Kanazawa H., Kobayashi E., Fujita J., Fukuda K.

    iScience (iScience)  24 ( 2 )  2021.02

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    Cell Biology; Metabolomics; Stem Cell Research

  • Fatty Acid Synthesis Is Indispensable for Survival of Human Pluripotent Stem Cells

    Tanosaki S., Tohyama S., Fujita J., Someya S., Hishiki T., Matsuura T., Nakanishi H., Ohto-Nakanishi T., Akiyama T., Morita Y., Kishino Y., Okada M., Tani H., Soma Y., Nakajima K., Kanazawa H., Sugimoto M., Ko M.S.H., Suematsu M., Fukuda K.

    iScience (iScience)  23 ( 9 )  2020.09

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    Biological Sciences; Cell Biology; Stem Cells Research; Proteomics; Metabolomics; Metabolic Flux Analysis

  • Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures

    Kurotsu S., Sadahiro T., Fujita R., Tani H., Yamakawa H., Tamura F., Isomi M., Kojima H., Yamada Y., Abe Y., Murakata Y., Akiyama T., Muraoka N., Harada I., Suzuki T., Fukuda K., Ieda M.

    Stem Cell Reports (Stem Cell Reports)  15 ( 3 ) 612 - 628 2020.09

    ISSN  22136711

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    In this article, Ieda and colleagues showed that a soft matrix, which is comparable with native myocardium, efficiently promoted cardiac reprogramming. This soft matrix enhanced cardiac reprogramming via inhibition of integrin, Rho/ROCK, actomyosin, and YAP/TAZ signaling and subsequent suppression of fibroblast programs, which were activated on conventional rigid substrates, thus demonstrating that mechanotransduction plays a critical role in cardiac reprogramming.

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