Sakamoto, Michiie

写真a

Affiliation

School of Medicine （Mita）

Position

Professor Emeritus

External Links
Page Top ▲
 

Research Areas 【 Display / hide 】

  • Life Science / Human pathology

Page Top ▲
 

Papers 【 Display / hide 】

  • Effectiveness of color correction on the quantitative analysis of histopathological images acquired by different whole-slide scanners

    Aziz M., Nakamura T., Yamaguchi M., Kiyuna T., Yamashita Y., Abe T., Hashiguchi A., Sakamoto M.

    Artificial Life and Robotics （Artificial Life and Robotics)  24 （ 1 ） 28 - 37 2019.03

    ISSN  14335298

    　View Summary

    © 2018, ISAROB. Advances in whole-slide scanning have led to research on digital pathology, including monitor-based diagnosis, feature quantification, and computer-aided diagnosis. The color variations due to the staining process and scanning device are a serious issue that should be solved in whole-slide imaging applications, and methods for color correction have been studied. Nevertheless, the effectiveness of color-correction methods in the quantitative analysis of histopathological images acquired by different whole-slide scanners (WSSs) has not been confirmed. In this work, several liver tissue samples were scanned by different WSSs, and a color-correction method for whole-slide images was applied to the digitized tissue specimens. A set of histological features were extracted from the histopathological images, both without and with color correction, to analyze the effectiveness of the color-correction method in feature quantification and cancer identification.

  • Accuracy of non-invasive scoring systems for diagnosing non-alcoholic steatohepatitis-related fibrosis: Multicenter validation study

    Itoh Y., Seko Y., Shima T., Nakajima T., Mizuno K., Kawamura Y., Akuta N., Ito K., Kawanaka M., Hiramatsu A., Sakamoto M., Harada K., Goto Y., Nakayama T., Kumada H., Okanoue T.

    Hepatology Research （Hepatology Research)  48 （ 13 ） 1099 - 1107 2018.12

    ISSN  13866346

    　View Summary

    © 2018 The Japan Society of Hepatology Aim: Hepatic fibrosis is the most important factor for estimating the prognosis of patients with non-alcoholic fatty liver disease (NAFLD). A novel non-invasive scoring system, the FM-fibro index, showed high accuracy in a pilot study. The purpose of this study was to validate the efficacy of the FM-fibro index in a multicenter cohort. Methods: Among 18 institutions, we analyzed 400 Japanese patients with biopsy-proven NAFLD. We evaluated the accuracies of the FM-fibro index, CA-fibro index, and European Liver Fibrosis (ELF) panel by area under the receiver operator characteristics curves (AUROC). The FM-fibro index includes three formulas for type IV collagen 7S, hyaluronic acid, and vascular cell adhesion molecule-1. Results: Among 400 patients, 205 were women, and the median age was 56 years. The histological distribution of Matteoni types 1, 2, 3, and 4 was 11, 40, 15, and 334, and the distribution of hepatic fibrosis stages 0 to 4 was 67, 183, 55, 63, and 32, respectively. The AUROCs of the FM-fibro index, CA-fibro index, and ELF panel for non-alcoholic steatohepatitis (NASH)-related fibrosis were 0.7178/0.7095/0.7065, 0.7093, and 0.7245, respectively. The sensitivity and specificity of the FM-fibro index for predicting NASH-related fibrosis was 0.5359/0.5210/0.4641 and 0.8333/0.8182/0.8788, respectively. The accuracy of the FM-fibro index was not significantly different from that of the CA-fibro index or the ELF panel. Conclusions: The FM-fibro index can predict NASH-related fibrosis with sufficient accuracy compared with previous scoring systems. Further analyses that verify the accuracy of the FM-fibro index to distinguish significant or advanced fibrosis in patients with NAFLD are awaited. (UMIN-CTR: UMIN000018158).

  • CD26 is a potential therapeutic target by humanized monoclonal antibody for the treatment of multiple myeloma

    Nishida H., Hayashi M., Morimoto C., Sakamoto M., Yamada T.

    Blood Cancer Journal （Blood Cancer Journal)  8 （ 11 ）  2018.11

    　View Summary

    © 2018, The Author(s). CD26, a 110-kDa transmembrane glycoprotein that is expressed on several tumor cells including malignant lymphoma, has been implicated in tumorigenesis: however, little is known regarding its role in multiple myeloma (MM). Recently, we identified CD26 expression on human osteoclasts (OCs) and demonstrated that humanized IgG1 monoclonal antibody targeting CD26, huCD26mAb, inhibits human OC differentiation. Herein, we show that CD26 expression was present on plasma cells in the bone marrow tissues of MM patients. In vitro immunostaining studies revealed that although CD26 expression was low or absent on MM cell lines cultured alone, it was intensely and uniformly expressed on MM cell lines co-cultured with OCs. The augmented CD26 expression in MM cells was exploited to enhance anti-MM efficacy of huCD26mAb via a substantial increase in antibody-dependent cytotoxicity (ADCC) but not complement-dependent cytotoxicity (CDC). Moreover, huCD26mAb in combination with novel agents synergistically enhanced huCD26mAb induced ADCC activity against CD26+ MM cells compared with each agent alone. huCD26mAb additionally reduced the ratio of the side population (SP) fraction in CD26+ MM cells by ADCC. Finally, huCD26mAb significantly reduced the MM tumor burden and OC formation in vivo. These results suggest that CD26 is a potential target molecule in MM and that huCD26mAb could act as a therapeutic agent.

  • Evaluation of pancreatic fibrosis with acoustic radiation force impulse imaging and automated quantification of pancreatic tissue components

    Fujita Y., Kitago M., Abe T., Itano O., Shinoda M., Abe Y., Yagi H., Hibi T., Ishii M., Nakano Y., Okuma K., Hashimoto M., Takeuchi A., Masugi Y., Jinzaki M., Sakamoto M., Kitagawa Y.

    Pancreas （Pancreas)  47 （ 10 ） 1277 - 1282 2018.11

    ISSN  08853177

    　View Summary

    © 2018 Wolters Kluwer Health, Inc. All rights reserved. Objectives The aim of this study was to determine whether computer-assisted digital analysis and acoustic radiation force impulse (ARFI) imaging were useful for assessing pancreatic fibrosis, and if ARFI imaging predicted postoperative pancreatic fistula (POPF). Methods Seventy-eight patients scheduled to undergo pancreatic resection were enrolled. Shear wave velocity (SWV) at the pancreatic neck was measured preoperatively using ARFI imaging. Pancreatic tissue components on a whole slide image were quantified using an automatic image processing software. The relationship between SWV, fibrotic tissue content, and POPF incidence and clinical severity was analyzed. Results The median collagen fiber, fatty tissue, and acinar cell contents were 11.6%, 8.5%, and 61.3%, respectively. Unlike fatty tissue, collagen fiber content and acinar cells were correlated with SWV (ρ = 0.440, P < 0.001 and ρ =-0.428, P < 0.001, respectively). Although collagen fiber content and SWV were associated with the overall incidence of POPF (P = 0.004 and 0.001, respectively), collagen fiber content and SWV had no statistical correlation with clinically relevant POPF (P = 0.268 and 0.052, respectively). Conclusions We objectively quantified the pancreatic tissue components using an automatic image processing software. Shear wave velocity was significantly related to collagen fiber content and suggests that ARFI imaging can be useful for evaluating pancreatic fibrosis.

  • Immunohistochemical visualization of the signature of activated Hedgehog signaling pathway in cutaneous epithelial tumors

    Tanese K., Emoto K., Kubota N., Fukuma M., Sakamoto M.

    Journal of Dermatology （Journal of Dermatology)  45 （ 10 ） 1181 - 1186 2018.10

    ISSN  03852407

    　View Summary

    © 2018 Japanese Dermatological Association Activation of the Hedgehog (HH) signaling pathway plays a critical role in the development of basal cell carcinoma (BCC). HH signaling activity is produced by nuclear translocation of transcription factors, glioma-associated oncogene homolog (GLI). Among three GLI subfamilies, GLI1 is the only full-length transcriptional activator, and its nuclear localization is recognized as a signature event in HH signaling activation. However, limited published work has investigated the nuclear staining of GLI1 protein in human tumor tissue samples by immunohistochemical analysis. In this study, we performed immunohistochemical staining of GLI1 in 382 cases of cutaneous epithelial tumors, including 196 BCC cases, using rabbit monoclonal antihuman GLI1 antibody (C68H3). As a result, 98.2% cases of BCC showed a diffuse and strong nuclear staining pattern regardless of the histological subtype. Positive staining was mainly restricted to the tumor nests, while the overlying epidermis was negative suggesting specificity of the antibody. In further analysis of other cutaneous epithelial tumors, 100% (4/4) cases of trichoblastoma, 15.1% (5/33) Bowen's disease, 3.5% (1/28) actinic keratosis and 12.5% (4/32) squamous cell carcinoma showed the nuclear staining pattern of GLI1. This suggested that HH signaling is also dysregulated in some other cutaneous malignant tumors. In conclusion, the C68H3 antibody is a useful tool for revealing activation of HH signaling in immunohistochemical analysis.

display all >>

Page Top ▲

Papers, etc., Registered in KOARA 【 Display / hide 】

display all >>

Page Top ▲

Reviews, Commentaries, etc. 【 Display / hide 】

Page Top ▲

Presentations 【 Display / hide 】

  • 肝細胞癌における多段階発癌とCyclase-associated protein-2(CAP2)の過剰発現

    SAKAMOTO MICHIIE

    ワークショップ　第64回日本癌学会学術総会 (札幌) , 

    2005.09
    , 

    Oral presentation (general), 日本癌学会

  • Overexpression of cyclase-associated protein 2 i multistage hepatocaricinogenesis

    MORI TAISUKE,Rie Shibata,Wenlin du,Makoto Chuma,Masahiro Gotoh,Setsuo Hirohashi,Michiie Sakamoto

    AACR annual meeting (anaheim USA) , 

    2005.04
    , 

    Poster presentation, AACR

  • オリゴヌクレオチドマイクロアレイを用いた非小細胞肺癌の網羅的遺伝子発現解析

    SAKAMOTO MICHIIE

    第44回日本肺癌学会総会、 (東京) , 

    2003.11
    , 

    Oral presentation (general)

  • 'DNA methyltransferase (DNMT) 1 protein expression is significantly increased in human hepatocellular carcinomas with malignant potential and may be a biological predictor of prognosis in hepatocellular carcinoma patients. The 54th Annual Meeting of the American Association for the Study of Liver Diseases, Boston, Massachusetts, USA, October 24-28, 2003'

    'Yoshimasa Saito, Yae Kanai, Tohru Nakagawa, Michiie Sakamoto, Hidetsugu Saito, Hiromasa Ishii, Setsuo Hirohashi.'

    'The 54th Annual Meeting of the American Association for the Study of Liver Diseases,' ('Boston, Massachusetts, USA,') , 

    2003.10
    , 

    Poster presentation

  • Expression profiling in multistage hepatocarcinogenesis; identification of HSP70 as a molecular marker of early hepatocellular carcinoma.

    'Makoto Chuma, Michiie Sakamoto, Shuhei Hige, Masahiro Asaka, Setsuo Hirohashi.'

    'The 54th Annual Meeting of the American Association for the Study of Liver Diseases,' ('Boston, Massachusetts, USA,') , 

    2003.10
    , 

    Poster presentation

display all >>

Page Top ▲

Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Establishing the molecular pathology-based subclassification of hepatocellular carcinoma.

    2014.04
    -
    2017.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

    　View Summary

    By analyzing cell signaling features and phenotype immunohistochemically, a new subclassification of hepatocellular carcinoma (HCC) that reflects the degree of malignancy was established. Activated MAPK signaling or overexpression of Glypican 3 as therapeutic target candidates were shown in biliary/stem cell marker-positive HCCs. The use of MRI to diagnose HCC with activated WNT signaling was also revealed. These findings are expected to be applied in the clinical practice.

Page Top ▲

Works 【 Display / hide 】

  • 学務委員会委員

    2003.10
    -
    2005.09
    , 

    Other

  • 教育委員会委員

    2003.10
    -
    2005.09
    , 

    Other

Page Top ▲
 

Courses Taught 【 Display / hide 】

  • GENERAL PATHOLOGY

    2024

  • GENERAL PATHOLOGY

    2023

  • DISEASES OF ORGAN SYSTEMS

    2023

  • ADVANCED PATHOLOGY

    2022

  • PATHOLOGY: SEMINAR

    2022

display all >>

Page Top ▲