Papers - Harada, Sei

Gender-specific association of early age-related macular degeneration with systemic and genetic factors in a Japanese population

Sasaki, Mariko, Harada, Sei, Kawasaki, Yumiko, Watanabe, Miki, Ito, Hidemi, Tanaka, Hideo, Takeuchi, Ayano, Tsubota, Kazuo, Takebayashi, Toru, Nishiwaki, Yuji, Kawasaki, Ryo

SCIENTIFIC REPORTS (Scientific Reports)  8 ( 1 )  2018.01

Research paper (scientific journal), Accepted,  ISSN  2045-2322

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© 2018 The Author(s). The Tsuruoka Metabolomics Cohort Study included subjects aged 35-74 years from participants in annual health check-up programs in Tsuruoka, Japan. The gender-specific associations of early age-related macular degeneration (AMD) with systemic and genetic factors was assessed cross-sectionally. Of these, 3,988 subjects had fundus photographs of sufficient quality, and early AMD was present in 12.3% and 10.3% of men and women, respectively. In men, higher levels of high-density lipoprotein cholesterol and lower levels of triglycerides were associated with increased odds of having early AMD after adjusting for potential risk factors (for each 1 mmol/L increase, odds ratio [OR]: 1.61 and 0.78, 95% confidence interval [CI]: 1.17-2.23 and 0.64-0.96, respectively). In women, higher levels of total cholesterol and low-density lipoprotein cholesterol were associated with increased risk of having early AMD (OR: 1.21 and 1.26, 95% CI: 1.01-1.44 and 1.03-1.53, respectively). Sub-analysis demonstrated that women with ARMS2 A69S polymorphisms had a stronger risk for early AMD (OR: 3.25, 95% CI: 2.10-5.04) than men (OR: 1.65, 95% CI: 1.02-2.69). Differential associations of early AMD with both systemic and genetic factors by sex were demonstrated in a Japanese cohort, which suggests that disease process of early AMD could be different by sex.

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Reliability of plasma polar metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry

Harada, Sei, Hirayama, Akiyoshi, Chan, Queenie, Kurihara, Ayako, Fukai, Kota, Iida, Miho, Kato, Suzuka, Sugiyama, Daisuke, Kuwabara, Kazuyo, Takeuchi, Ayano, Akiyama, Miki, Okamura, Tomonori, Ebbels, Timothy M. D., Elliott, Paul, Tomita, Masaru, Sato, Asako, Suzuki, Chizuru, Sugimoto, Masahiro, Soga, Tomoyoshi, Takebayashi, Toru

PLOS ONE (PLoS ONE)  13 ( 1 )  2018.01

Research paper (scientific journal), Joint Work,  ISSN  1932-6203

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© 2018 Harada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Background Cohort studies with metabolomics data are becoming more widespread, however, large-scale studies involving 10,000s of participants are still limited, especially in Asian populations. Therefore, we started the Tsuruoka Metabolomics Cohort Study enrolling 11,002 community-dwelling adults in Japan, and using capillary electrophoresis-mass spectrometry (CE-MS) and liquid chromatography–mass spectrometry. The CE-MS method is highly amenable to absolute quantification of polar metabolites, however, its reliability for large-scale measurement is unclear. The aim of this study is to examine reproducibility and validity of large-scale CE-MS measurements. In addition, the study presents absolute concentrations of polar metabolites in human plasma, which can be used in future as reference ranges in a Japanese population. Methods Metabolomic profiling of 8,413 fasting plasma samples were completed using CE-MS, and 94 polar metabolites were structurally identified and quantified. Quality control (QC) samples were injected every ten samples and assessed throughout the analysis. Inter- and intra-batch coefficients of variation of QC and participant samples, and technical intraclass correlation coefficients were estimated. Passing-Bablok regression of plasma concentrations by CE-MS on serum concentrations by standard clinical chemistry assays was conducted for creatinine and uric acid. Results and conclusions In QC samples, coefficient of variation was less than 20% for 64 metabolites, and less than 30% for 80 metabolites out of the 94 metabolites. Inter-batch coefficient of variation was less than 20% for 81 metabolites. Estimated technical intraclass correlation coefficient was above 0.75 for 67 metabolites. The slope of Passing-Bablok regression was estimated as 0.97 (95% confidence interval: 0.95, 0.98) for creatinine and 0.95 (0.92, 0.96) for uric acid. Compared to published data from other large cohort measurement platforms, reproducibility of metabolites common to the platforms was similar to or better than in the other studies. These results show that our CE-MS platform is suitable for conducting large-scale epidemiological studies.

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Metabolic Profiling of Total Physical Activity and Sedentary Behavior in Community-Dwelling Men

Fukai, Kota, Harada, Sei, Iida, Miho, Kurihara, Ayako, Takeuchi, Ayano, Kuwabara, Kazuyo, Sugiyama, Daisuke, Okamura, Tomonori, Akiyama, Miki, Nishiwaki, Yuji, Oguma, Yuko, Suzuki, Asako, Suzuki, Chizuru, Hirayama, Akiyoshi, Sugimoto, Masahiro, Soga, Tomoyoshi, Tomita, Masaru, Takebayashi, Toru

PLOS ONE 11 ( 10 )  2016.10

Research paper (scientific journal), Joint Work, Accepted,  ISSN  1932-6203

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Profiling of plasma metabolites in postmenopausal women with metabolic syndrome

Iida, Miho, Harada, Sei, Kurihara, Ayako, Fukai, Kota, Kuwabara, Kazuyo, Sugiyama, Daisuke, Takeuchi, Ayano, Okamura, Tomonori, Akiyama, Miki, Nishiwaki, Yuji, Suzuki, Asako, Hirayama, Akiyoshi, Sugimoto, Masahiro, Soga, Tomoyoshi, Tomita, Masaru, Banno, Kouji, Aoki, Daisuke, Takebayashi, Toru

MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY 23 ( 7 ) 749 - 758 2016.07

Research paper (scientific journal), Joint Work,  ISSN  1072-3714

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Metabolomic profiling reveals novel biomarkers of alcohol intake and alcohol-induced liver injury in community-dwelling men (vol 21, pg 18, 2016)

Harada, Sei, Takebayashi, Toru, Kurihara, Ayako, Akiyama, Miki, Suzuki, Asako, Hatakeyama, Yoko, Sugiyama, Daisuke, Kuwabara, Kazuyo, Takeuchi, Ayano, Okamura, Tomonori, Nishiwaki, Yuji, Tanaka, Taichiro, Hirayama, Akiyoshi, Sugimoto, Masahiro, Soga, Tomoyoshi, Tomita, Masaru

ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE 21 ( 4 ) 283 - 284 2016.07

Research paper (scientific journal), Joint Work,  ISSN  1342-078X

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Metabolomic profiling reveals novel biomarkers of alcohol intake and alcohol-induced liver injury in community-dwelling men

Harada, Sei, Takebayashi, Toru, Kurihara, Ayako, Akiyama, Miki, Suzuki, Asako, Hatakeyama, Yoko, Sugiyama, Daisuke, Kuwabara, Kazuyo, Takeuchi, Ayano, Okamura, Tomonori, Nishiwaki, Yuji, Tanaka, Taichiro, Hirayama, Akiyoshi, Sugimoto, Masahiro, Soga, Tomoyoshi, Tomita, Masaru

ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE 21 ( 1 ) 18 - 26 2016.01

Research paper (scientific journal),  ISSN  1342-078X

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Relationship between Non-High-Density Lipoprotein Cholesterol and Low-Density Lipoprotein Cholesterol in the General Population: the KOBE Study and Tsuruoka Metabolomic Cohort Study

Kuwabara, Kazuyo, Harada, Sei, Sugiyama, Daisuke, Kurihara, Ayako, Kubota, Yoshimi, Higashiyama, Aya, Hirata, Takumi, Nishida, Yoko, Kawasaki, Midori, Takebayashi, Toru, Okamura, Tomonori

JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS 23 ( 4 ) 477 - 490 2016

Research paper (scientific journal), Joint Work,  ISSN  1340-3478

Effects of processing and storage conditions on charged metabolomic profiles in blood

Hirayama, Akiyoshi, Sugimoto, Masahiro, Suzuki, Asako, Hatakeyama, Yoko, Enomoto, Ayame, Harada, Sei, Soga, Tomoyoshi, Tomita, Masaru, Takebayashi, Toru

ELECTROPHORESIS 36 ( 18 ) 2148 - 2155 2015.09

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0173-0835

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Circulating AIM as an Indicator of Liver Damage and Hepatocellular Carcinoma in Humans

Yamazaki, Tomoko, Mori, Mayumi, Arai, Satoko, Tateishi, Ryosuke, Abe, Masanori, Ban, Mihoko, Nishijima, Akemi, Maeda, Maki, Asano, Takeharu, Kai, Toshihiro, Izumino, Kiyohiro, Takahashi, Jun, Aoyama, Kayo, Harada, Sei, Takebayashi, Toru, Gunji, Toshiaki, Ohnishi, Shin, Seto, Shinji, Yoshida, Yukio, Hiasa, Yoichi, Koike, Kazuhiko, Yamamura, Ken-ichi, Inoue, Ken-ichiro, Miyazaki, Toru

PLOS ONE 9 ( 10 )  2014.10

Research paper (scientific journal), Accepted,  ISSN  1932-6203

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Gender difference in the relationships between vision and hearing impairments and negative well-being

Harada, Sei, Nishiwaki, Yuji, Michikawa, Takehiro, Kikuchi, Yuriko, Iwasawa, Satoko, Nakano, Makiko, Ishigami, Ai, Saito, Hideyuki, Takebayashi, Toru

PREVENTIVE MEDICINE 47 ( 4 ) 433 - 437 2008.10

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0091-7435

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