Papers - Shiomi, Haruhiko

Human PIWI (HIWI) is an azoospermia factor

Hasuwa Hidetoshi, Ishino Kyoko, Siomi Haruhiko

Science China Life Sciences    1 - 3 2017.08

ISSN  1674-7305

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Deep sequencing and high-throughput analysis of PIWI-associated small RNAs

Iwasaki Yuka W., Ishino Kyoko, Siomi Haruhiko

Methods  2017.03

ISSN  1046-2023

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<p>Small RNAs are now known to be major regulatory factors of gene expression. Emerging methods based on deep-sequencing have enabled the analysis of small RNA expression in a high-throughput manner, leading to the identification of large numbers of small RNAs in various species. Moreover, profiling small RNA data together with transcriptome data enables transcriptional and post-transcriptional regulation mediated by small RNAs to be hypothesized. Here, we isolated PIWIL1 (MIWI)-associated small RNAs from mouse testes, and performed small RNA-seq analysis. In addition, directional RNA-seq was performed using Piwil1 mutant mouse testes. Using these data, we describe protocols for analyzing small RNA-seq reads to obtain profiles of small RNAs associated with PIWI proteins. We also present bioinformatic protocols for analyzing RNA-seq reads that aim to annotate expression of piRNA clusters and identify genes regulated by piRNAs.</p>

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Mobile elements control stem cell potency

Hasuwa Hidetoshi, Siomi Haruhiko

Science 355 ( 6325 ) 581 - 582 2017.02

ISSN  0036-8075

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Identification of mouse piRNA pathway components using anti-MIWI2 antibodies

Hirano Takamasa, Hasuwa Hidetoshi, Siomi Haruhiko

  205 - 216 2017

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<p>The mouse testis has served as a popular model system to study a wide range of biological processes, including germcell development, meiosis, epigenetic changes of chromatin, transposon silencing, and small RNA-mediated epigenetic modifications. PIWI-interacting RNAs (piRNAs) are a class of small RNAs that are almost exclusively expressed in animal gonads. They repress transposons by forming effector complexes with PIWI proteins to maintain genome integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci. We then describe how to use the antibodies to isolate associated complexes and to detect MIWI2 immunohistochemically.</p>

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Crystal Structure of Silkworm PIWI-Clade Argonaute Siwi Bound to piRNA

Matsumoto Naoki, Nishimasu Hiroshi, Sakakibara Kazuhiro, Nishida Kazumichi M., Hirano Takamasa, Ishitani Ryuichiro, Siomi Haruhiko, Siomi Mikiko C., Nureki Osamu

Cell 167 ( 2 ) 484 - 497.e9 2016.10

ISSN  0092-8674

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<p>PIWI-clade Argonaute proteins associate with PIWI-interacting RNAs (piRNAs) and silence transposable elements in animal gonads. Here, we report the crystal structure of a silkworm PIWI-clade Argonaute, Siwi, bound to the endogenous piRNA, at 2.4 Å resolution. Siwi adopts a bilobed architecture consisting of N-PAZ and MID-PIWI lobes, in which the 5′ and 3′ ends of the bound piRNA are anchored by the MID-PIWI and PAZ domains, respectively. A structural comparison of Siwi with AGO-clade Argonautes reveals notable differences in their nucleic-acid-binding channels, likely reflecting the distinct lengths of their guide RNAs and their mechanistic differences in guide RNA loading and cleavage product release. In addition, the structure reveals that Siwi and prokaryotic, but not eukaryotic, AGO-clade Argonautes share unexpected similarities, such as metal-dependent 5′-phosphate recognition and a potential structural transition during the catalytic-tetrad formation. Overall, this study provides a critical starting point toward a mechanistic understanding of piRNA-mediated transposon silencing.</p>

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Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells

Sumiyoshi Tetsutaro, Sato Kaoru, Yamamoto Hitomi, Iwasaki Yuka W., Siomi Haruhiko, Siomi Mikiko C.

Genes and Development 30 ( 14 ) 1617 - 1622 2016.07

ISSN  0890-9369

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<p>In Drosophila germ cells, PIWI-interacting RNAs (piR-NAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific pingpong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila.</p>

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Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian

Shibata, Norito, Kashima, Makoto, Ishiko, Taisuke, Nishimura, Osamu, Rouhana, Labib, Misaki, Kazuyo, Yonemura, Shigenobu, Saito, Kuniaki, Siomi, Haruhiko, Siomi, Mikiko C., Agata, Kiyokazu

DEVELOPMENTAL CELL 37 ( 3 ) 226 - 237 2016.05

Research paper (scientific journal), Joint Work,  ISSN  1534-5807

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Misprocessed tRNA response targets piRNA clusters

Yamanaka, Soichiro, Siomi, Haruhiko

EMBO JOURNAL 34 ( 24 ) 2988 - 2989 2015.12

Research paper (scientific journal), Joint Work,  ISSN  0261-4189

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piRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals

Parrish, Nicholas F., Fujino, Kan, Shiromoto, Yusuke, Iwasaki, Yuka W., Ha, Hongseok, Xing, Jinchuan, Makino, Akiko, Kuramochi-Miyagawa, Satomi, Nakano, Toru, Siomi, Haruhiko, Honda, Tomoyuki, Tomonaga, Keizo

RNA 21 ( 10 ) 1691 - 1703 2015.10

Research paper (scientific journal), Joint Work,  ISSN  1355-8382

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Krimper enforces an antisense bias on piRNA pools by binding AGO3 in the Drosophila germline.

Sato, K., Iwasaki, YW., Shibuya, A., Carninci, C., Tsuchizawa, Y., Ishizu, H., Siomi. MC., and Siomi, H.

Molecular cell (Cell Press)  59   553 - 563 2015.07

Research paper (scientific journal), Accepted

Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb

Ishizu, Hirotsugu, Iwasaki, Yuka W., Hirakata, Shigeki, Ozaki, Haruka, Iwasaki, Wataru, Siomi, Haruhiko, Siomi, Mikiko C.

CELL REPORTS 12 ( 3 ) 429 - 440 2015.07

Research paper (scientific journal),  ISSN  2211-1247

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Phased piRNAs tackle transposons

Siomi, Haruhiko, Siomi, Mikiko C.

SCIENCE 348 ( 6236 ) 756 - 757 2015.05

Research paper (scientific journal),  ISSN  0036-8075

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Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom

Matsumoto, Naoki, Sato, Kaoru, Nishimasu, Hiroshi, Namba, Yurika, Miyakubi, Kana, Dohmae, Naoshi, Ishitani, Ryuichiro, Siomi, Haruhiko, Siomi, Mikiko C., Nureki, Osamu

Cell Reports 11 ( 3 ) 366 - 375 2015.04

Research paper (scientific journal),  ISSN  2211-1247

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Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development

Lin, Zachary Yu-Ching, Hirano, Takamasa, Shibata, Shinsuke, Seki, Naomi M., Kitajima, Ryunosuke, Sedohara, Ayako, Siomi, Mikiko C., Sasaki, Erika, Siomi, Haruhiko, Imamura, Masanori, Okano, Hideyuki

DEVELOPMENTAL BIOLOGY 400 ( 1 ) 43 - 58 2015.04

Research paper (scientific journal),  ISSN  0012-1606

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Small RNAs: Artificial piRNAs for Transcriptional Silencing

Hirano, Takamasa, Siomi, Haruhiko

CURRENT BIOLOGY 25 ( 7 ) R280 - R283 2015.03

Research paper (scientific journal),  ISSN  0960-9822

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Respective Functions of Two Distinct Siwi Complexes Assembled during PIWI-Interacting RNA Biogenesis in Bombyx Germ Cells

Nishida, Kazumichi M., Iwasaki, Yuka W., Murota, Yukiko, Nagao, Akihiro, Mannen, Taro, Kato, Yumiko, Siomi, Haruhiko, Siomi, Mikiko C.

CELL REPORTS 10 ( 2 ) 193 - 203 2015.01

Research paper (scientific journal),  ISSN  2211-1247

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PIWI-Interacting RNA: Its Biogenesis and Functions

Iwasaki, Yuka W., Siomi, Mikiko C., Siomi, Haruhiko

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 84 84   405 - 433 2015

Research paper (scientific journal),  ISSN  0066-4154

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miRNA Regulatory Ecosystem in Early Development

Iwasaki, Yuka W., Siomi, Haruhiko

MOLECULAR CELL 56 ( 5 ) 615 - 616 2014.12

Research paper (scientific journal),  ISSN  1097-2765

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Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate

Hirano, Takamasa, Iwasaki, Yuka W., Lin, Zachary Yu-Ching, Imamura, Masanori, Seki, Naomi M., Sasaki, Erika, Saito, Kuniaki, Okano, Hideyuki, Siomi, Mikiko C., Siomi, Haruhiko

RNA 20 ( 8 ) 1223 - 1237 2014.08

Research paper (scientific journal),  ISSN  1355-8382

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piRNA clusters and open chromatin structure

Yamanaka, Soichiro, Siomi, Mikiko C., Siomi, Haruhiko

MOBILE DNA 5   1282 - 1291 2014.08

Research paper (scientific journal),  ISSN  1759-8753

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Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly

Murota, Yukiko, Ishizu, Hirotsugu, Nakagawa, Shinichi, Iwasaki, Yuka W., Shibata, Shinsuke, Kamatani, Miharu K., Saito, Kuniaki, Okano, Hideyuki, Siomi, Haruhiko, Siomi, Mikiko C.

CELL REPORTS 8 ( 1 ) 103 - 113 2014.07

Research paper (scientific journal),  ISSN  2211-1247

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It's time to exploit your favorite quirky organism with new technologies

Siomi, Haruhiko

EMBO REPORTS 15 ( 6 ) 620 - 621 2014.06

Research paper (scientific journal),  ISSN  1469-221X

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DmGTSF1 is necessary for Piwi-piRISC-mediated transcriptional transposon silencing in the Drosophila ovary

Ohtani, Hitoshi, Iwasaki, Yuka W., Shibuya, Aoi, Siomi, Haruhiko, Siomi, Mikiko C., Saito, Kuniaki

GENES &amp; DEVELOPMENT 27 ( 15 ) 1656 - 1661 2013.08

Research paper (scientific journal),  ISSN  0890-9369

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Roles of R2D2, a Cytoplasmic D2 Body Component, in the Endogenous siRNA Pathway in Drosophila

Nishida, Kazumichi M., Miyoshi, Keita, Ogino, Akiyo, Miyoshi, Tomohiro, Siomi, Haruhiko, Siomi, Mikiko C.

MOLECULAR CELL 49 ( 4 ) 680 - 691 2013.02

Research paper (scientific journal),  ISSN  1097-2765

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Biology of PIWI-interacting RNAs: new insights into biogenesis and function inside and outside of germlines

Ishizu, Hirotsugu, Siomi, Haruhiko, Siomi, Mikiko C.

GENES &amp; DEVELOPMENT 26 ( 21 ) 2361 - 2373 2012.11

Research paper (scientific journal),  ISSN  0890-9369

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Structure and function of Zucchini endoribonuclease in piRNA biogenesis

Nishimasu, Hiroshi, Ishizu, Hirotsugu, Saito, Kuniaki, Fukuhara, Satoshi, Kamatani, Miharu K., Bonnefond, Luc, Matsumoto, Naoki, Nishizawa, Tomohiro, Nakanaga, Keita, Aoki, Junken, Ishitani, Ryuichiro, Siomi, Haruhiko, Siomi, Mikiko C., Nureki, Osamu

NATURE 491 ( 7423 ) 284 - U157 2012.11

Research paper (scientific journal),  ISSN  0028-0836

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Chromatin-associated RNA interference components contribute to transcriptional regulation in Drosophila

Cernilogar, Filippo M., Onorati, Maria Cristina, Kothe, Greg O., Burroughs, A. Maxwell, Parsi, Krishna Mohan, Breiling, Achim, Lo Sardo, Federica, Saxena, Alka, Miyoshi, Keita, Siomi, Haruhiko, Siomi, Mikiko C., Carninci, Piero, Gilmour, David S., Corona, Davide F. V., Orlando, Valerio

NATURE 480 ( 7377 ) 391 - U151 2011.12

Research paper (scientific journal),  ISSN  0028-0836

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Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOC

Sato, Kaoru, Nishida, Kazumichi M., Shibuya, Aoi, Siomi, Mikiko C., Siomi, Haruhiko

GENES &amp; DEVELOPMENT 25 ( 22 ) 2361 - 2373 2011.11

Research paper (scientific journal),  ISSN  0890-9369

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Stress Signaling Etches Heritable Marks on Chromatin

Siomi, Haruhiko, Siomi, Mikiko C.

CELL 145 ( 7 ) 1005 - 1007 2011.06

Research paper (scientific journal),  ISSN  0092-8674

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Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis

Nagao, Akihiro, Mituyama, Toutai, Huang, Haidong, Chen, Dahua, Siomi, Mikiko C., Siomi, Haruhiko

RNA-A PUBLICATION OF THE RNA SOCIETY 16 ( 12 ) 2503 - 2515 2010.12

Research paper (scientific journal),  ISSN  1355-8382

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Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila

Saito, Kuniaki, Ishizu, Hirotsugu, Komai, Miharu, Kotani, Hazuki, Kawamura, Yoshinori, Nishida, Kazumichi M., Siomi, Haruhiko, Siomi, Mikiko C.

GENES &amp; DEVELOPMENT 24 ( 22 ) 2493 - 2498 2010.11

Research paper (scientific journal),  ISSN  0890-9369

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piRNA-mediated silencing in Drosophila germlines

Siomi, Mikiko C., Miyoshi, Tomohiro, Siomi, Haruhiko

SEMINARS IN CELL &amp; DEVELOPMENTAL BIOLOGY 21 ( 7 ) 754 - 759 2010.09

Research paper (scientific journal),  ISSN  1084-9521

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Many ways to generate microRNA-like small RNAs: non-canonical pathways for microRNA production

Miyoshi, Keita, Miyoshi, Tomohiro, Siomi, Haruhiko

MOLECULAR GENETICS AND GENOMICS 284 ( 2 ) 95 - 103 2010.08

Research paper (scientific journal),  ISSN  1617-4615

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A direct role for Hsp90 in pre-RISC formation in Drosophila

Miyoshi, Tomohiro, Takeuchi, Akiko, Siomi, Haruhiko, Siomi, Mikiko C.

NATURE STRUCTURAL &amp; MOLECULAR BIOLOGY 17 ( 8 ) 1024 - 1026 2010.08

Research paper (scientific journal),  ISSN  1545-9985

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Posttranscriptional Regulation of MicroRNA Biogenesis in Animals

Siomi, Haruhiko, Siomi, Mikiko C.

MOLECULAR CELL 38 ( 3 ) 323 - 332 2010.05

Research paper (scientific journal),  ISSN  1097-2765

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Is canalization more than just a beautiful idea?

Sato, Kaoru, Siomi, Haruhiko

GENOME BIOLOGY 11 ( 3 ) 754 - 759 2010

Research paper (scientific journal),  ISSN  1474-7596

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Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlines

Nishida, Kazumichi M., Okada, Tomoko N., Kawamura, Takeshi, Mituyama, Toutai, Kawamura, Yoshinori, Inagaki, Sachi, Huang, Haidong, Chen, Dahua, Kodama, Tatsuhiko, Siomi, Haruhiko, Siomi, Mikiko C.

EMBO JOURNAL 28 ( 24 ) 3820 - 3831 2009.12

Research paper (scientific journal),  ISSN  0261-4189

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A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila

Saito, Kuniaki, Inagaki, Sachi, Mituyama, Toutai, Kawamura, Yoshinori, Ono, Yukiteru, Sakota, Eri, Kotani, Hazuki, Asai, Kiyoshi, Siomi, Haruhiko, Siomi, Mikiko C.

NATURE 461 ( 7268 ) 1296 - U135 2009.10

Research paper (scientific journal),  ISSN  0028-0836

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RISC hitches onto endosome trafficking

Siomi, Haruhiko, Siomi, Mikiko C.

NATURE CELL BIOLOGY 11 ( 9 ) 1049 - 1051 2009.09

Research paper (scientific journal),  ISSN  1465-7392

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Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway

Miyoshi, Keita, Okada, Tomoko N., Siomi, Haruhiko, Siomi, Mikiko C.

RNA-A PUBLICATION OF THE RNA SOCIETY 15 ( 7 ) 1282 - 1291 2009.07

Research paper (scientific journal),  ISSN  1355-8382

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On the road to reading the RNA-interference code

Siomi, Haruhiko, Siomi, Mikiko C.

NATURE 457 ( 7228 ) 396 - 404 2009.01

Research paper (scientific journal),  ISSN  0028-0836

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Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells

Kawamura, Yoshinori, Saito, Kuniaki, Kin, Taishin, Ono, Yukiteru, Asai, Kiyoshi, Sunohara, Takafumi, Okada, Tomoko N., Siomi, Mikiko C., Siomi, Haruhiko

NATURE 453 ( 7196 ) 793 - U5 2008.06

Research paper (scientific journal),  ISSN  0028-0836

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Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing

Azuma-Mukai, Asuka, Oguri, Hideo, Mituyama, Toutai, Qian, Zhi Rong, Asai, Kiyoshi, Siomi, Haruhiko, Siomi, Mikiko C.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 ( 23 ) 7964 - 7969 2008.06

Research paper (scientific journal),  ISSN  0027-8424

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Interactions between transposable elements and Argonautes have (probably) been shaping the evolution Drosophila genome throughout

Siomi, Haruhiko, Siomi, Mikiko C.

CURRENT OPINION IN GENETICS &amp; DEVELOPMENT 18 ( 2 ) 181 - 187 2008.04

Research paper (scientific journal),  ISSN  0959-437X

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HOW TO DEFINE TARGETS FOR SMALL GUIDE RNAS IN RNA SILENCING: A BIOCHEMICAL APPROACH

Siomi, Mikiko C., Nishida, Kazumichi M., Siomi, Haruhiko

RNA TURNOVER IN EUKARYOTES: ANALYSIS OF SPECIALIZED AND QUALITY CONTROL RNA DECAY PATHWAYS 449   345 - 355 2008

Research paper (scientific journal),  ISSN  0076-6879

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Pimet, the Drosophila homolog of HEN1, mediates 2 '-O-methylation of PIWI-interacting RNAs at their 3 ' ends

Saito, Kuniaki, Sakaguchi, Yuriko, Suzuki, Takeo, Suzuki, Tsutomu, Siomi, Haruhiko, Siomi, Mikiko C.

GENES &amp; DEVELOPMENT 21 ( 13 ) 1603 - 1608 2007.07

Research paper (scientific journal),  ISSN  0890-9369

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Expanding RNA physiology: microRNAs in a unicellular organism

Siomi, Haruhiko, Siomi, Mikiko C.

GENES &amp; DEVELOPMENT 21 ( 10 ) 1153 - 1156 2007.05

Research paper (scientific journal),  ISSN  0890-9369

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A slicer-mediated mechanism for repeat-associated siRNA 5 ' end formation in Drosophila

Gunawardane, Lalith S., Saito, Kuniaki, Nishida, Kazumichi M., Miyoshi, Keita, Kawamura, Yoshinori, Nagami, Tomoko, Siomi, Haruhiko, Siomi, Mikiko C.

SCIENCE 315 ( 5818 ) 1587 - 1590 2007.03

Research paper (scientific journal),  ISSN  0036-8075

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Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome

Saito, Kuniaki, Nishida, Kazumichi M., Mori, Tomoko, Kawamura, Yoshinori, Miyoshi, Keita, Nagami, Tomoko, Siomi, Haruhiko, Siomi, Mikiko C.

GENES &amp; DEVELOPMENT 20 ( 16 ) 2214 - 2222 2006.08

Research paper (scientific journal),  ISSN  0890-9369

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Slicer function of Drosophila Argonautes and its involvement in RISC formation

Miyoshi, K, Tsukumo, H, Nagami, T, Siomi, H, Siomi, MC

GENES &amp; DEVELOPMENT 19 ( 23 ) 2837 - 2848 2005.12

Research paper (scientific journal),  ISSN  0890-9369

Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells

Saito, K, Ishizuka, A, Siomi, H, Siomi, MC

PLOS BIOLOGY 3 ( 7 ) 1202 - 1212 2005.07

Research paper (scientific journal),  ISSN  1544-9173

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Distinct roles for argonaute proteins in small RNA-directed RNA cleavage pathways

Okamura, K, Ishizuka, A, Siomi, H, Siomi, MC

GENES &amp; DEVELOPMENT 18 ( 14 ) 1655 - 1666 2004.07

Research paper (scientific journal),  ISSN  0890-9369

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RNA interference: A new mechanism by which FMRP acts in the normal brain? What can drosophila teach us?

Siomi, H, Ishizuka, A, Siomi, MC

MENTAL RETARDATION AND DEVELOPMENTAL DISABILITIES RESEARCH REVIEWS 10 ( 1 ) 68 - 74 2004

Research paper (scientific journal),  ISSN  1080-4013

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A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins

Ishizuka, A, Siomi, MC, Siomi, H

GENES &amp; DEVELOPMENT 16 ( 19 ) 2497 - 2508 2002.10

Research paper (scientific journal),  ISSN  0890-9369

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A role for the Drosophila fragile X-related gene in circadian output

Inoue, SB, Shimoda, M, Nishinokubi, I, Siomi, MC, Okamura, M, Nakamura, A, Kobayashi, S, Ishida, N, Siomi, H

CURRENT BIOLOGY 12 ( 15 ) 1331 - 1335 2002.08

Research paper (scientific journal),  ISSN  0960-9822

The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExX-box helicase to direct RNAi in C-elegans

Tabara, H, Yigit, E, Siomi, H, Mello, CC

CELL 109 ( 7 ) 861 - 871 2002.06

Research paper (scientific journal),  ISSN  0092-8674

Transportin: Nuclear transport receptor of a novel nuclear protein import pathway

Nakielny, S, Siomi, MC, Siomi, H, Michael, WM, Pollard, V, Dreyfuss, G

EXPERIMENTAL CELL RESEARCH 229 ( 2 ) 261 - 266 1996.12

Research paper (scientific journal),  ISSN  0014-4827

Specific sequences in the fragile X syndrome protein FMR1 and the FXR proteins mediate their binding to 60S ribosomal subunits and the interactions among them

Siomi, MC, Zhang, Y, Siomi, H, Dreyfuss, G

MOLECULAR AND CELLULAR BIOLOGY 16 ( 7 ) 3825 - 3832 1996.07

Research paper (scientific journal),  ISSN  0270-7306

Molecular characterization of the protein products of the fragile X syndrome gene and the survival of motor neurons gene

Liu, Q, Siomi, H, Siomi, MC, Fischer, U, Zhang, Y, Wan, L, Dreyfuss, G

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 61   689 - 697 1996

Research paper (scientific journal),  ISSN  0091-7451

FXR1, AN AUTOSOMAL HOMOLOG OF THE FRAGILE-X MENTAL-RETARDATION GENE

SIOMI, MC, SIOMI, H, SAUER, WH, SRINIVASAN, S, NUSSBAUM, RL, DREYFUSS, G

EMBO JOURNAL 14 ( 11 ) 2401 - 2408 1995.06

Research paper (scientific journal),  ISSN  0261-4189

A NUCLEAR-LOCALIZATION DOMAIN IN THE HNRNP A1 PROTEIN

SIOMI, H, DREYFUSS, G

JOURNAL OF CELL BIOLOGY 129 ( 3 ) 551 - 560 1995.05

Research paper (scientific journal),  ISSN  0021-9525

ESSENTIAL ROLE FOR KH DOMAINS IN RNA-BINDING - IMPAIRED RNA-BINDING BY A MUTATION IN THE KH DOMAIN OF FMR1 THAT CAUSES FRAGILE-X SYNDROME

SIOMI, H, CHOI, MY, SIOMI, MC, NUSSBAUM, RL, DREYFUSS, G

CELL 77 ( 1 ) 33 - 39 1994.04

Research paper (scientific journal),  ISSN  0092-8674

LONG CELLULAR REPEATS FLANKING A DEFECTIVE HTLV-I PROVIRUS - IMPLICATION FOR SITE-TARGETED INTEGRATION

KUBOTA, S, FURUTA, R, MAKI, M, SIOMI, H, HATANAKA, M

ONCOGENE 8 ( 10 ) 2873 - 2877 1993.10

Research paper (scientific journal),  ISSN  0950-9232

THE PROTEIN PRODUCT OF THE FRAGILE-X GENE, FMR1, HAS CHARACTERISTICS OF AN RNA-BINDING PROTEIN

SIOMI, H, SIOMI, MC, NUSSBAUM, RL, DREYFUSS, G

CELL 74 ( 2 ) 291 - 298 1993.07

Research paper (scientific journal),  ISSN  0092-8674

THE PREMESSENGER RNA-BINDING K-PROTEIN CONTAINS A NOVEL EVOLUTIONARILY CONSERVED MOTIF

SIOMI, H, MATUNIS, MJ, MICHAEL, WM, DREYFUSS, G

NUCLEIC ACIDS RESEARCH 21 ( 5 ) 1193 - 1198 1993.03

Research paper (scientific journal),  ISSN  0305-1048

HTLV-1 P27REX STABILIZES HUMAN INTERLEUKIN-2 RECEPTOR ALPHA-CHAIN MESSENGER-RNA

KANAMORI, H, SUZUKI, N, SIOMI, H, NOSAKA, T, SATO, A, SABE, H, HATANAKA, M, HONJO, T

EMBO JOURNAL 9 ( 12 ) 4161 - 4166 1990.12

Research paper (scientific journal),  ISSN  0261-4189

FUNCTIONAL COMPARISON OF TRANSACTIVATION BY HUMAN RETROVIRUS REV AND REX GENES

SAKAI, H, SIOMI, H, SHIDA, H, SHIBATA, R, KIYOMASU, T, ADACHI, A

JOURNAL OF VIROLOGY 64 ( 12 ) 5833 - 5839 1990.12

Research paper (scientific journal),  ISSN  0022-538X

EFFECTS OF A HIGHLY BASIC REGION OF HUMAN-IMMUNODEFICIENCY-VIRUS TAT PROTEIN ON NUCLEOLAR LOCALIZATION

SIOMI, H, SHIDA, H, MAKI, M, HATANAKA, M

JOURNAL OF VIROLOGY 64 ( 4 ) 1803 - 1807 1990.04

Research paper (scientific journal),  ISSN  0022-538X

NUCLEOLAR TARGETING SIGNAL OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I REX-ENCODED PROTEIN IS ESSENTIAL FOR CYTOPLASMIC ACCUMULATION OF UNSPLICED VIRAL MESSENGER-RNA

NOSAKA, T, SIOMI, H, ADACHI, Y, ISHIBASHI, M, KUBOTA, S, MAKI, M, HATANAKA, M

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86 ( 24 ) 9798 - 9802 1989.12

Research paper (scientific journal),  ISSN  0027-8424

SEQUENCE REQUIREMENTS FOR NUCLEOLAR LOCALIZATION OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-L PX PROTEIN, WHICH REGULATES VIRAL-RNA PROCESSING

SIOMI, H, SHIDA, H, NAM, SH, NOSAKA, T, MAKI, M, HATANAKA, M

CELL 55 ( 2 ) 197 - 209 1988.10

Research paper (scientific journal),  ISSN  0092-8674