岩崎 由香 (イワサキ ユカ)

Iwasaki, Yuka

写真a

所属(所属キャンパス)

医学部 分子生物学教室 (信濃町)

職名

准教授

メールアドレス

メールアドレス

HP

経歴 【 表示 / 非表示

  • 2020年05月
    -
    継続中

    慶應義塾大学, 医学部, 准教授

  • 2016年08月
    -
    2020年04月

    慶應義塾大学, 医学部, 専任講師

  • 2012年08月
    -
    2016年07月

    慶應義塾大学, 医学部, 助教

  • 2011年04月
    -
    2012年07月

    慶應義塾大学, 医学部, 特任助教

学歴 【 表示 / 非表示

  • 2011年03月

    慶應義塾大学大学院, 政策・メディア研究科

    大学院, 修了

  • 2006年03月

    慶應義塾大学, 環境情報学部

    大学, 卒業

学位 【 表示 / 非表示

  • 博士(学術), 慶應義塾大学, 課程, 2011年03月

 

研究分野 【 表示 / 非表示

  • ゲノム生物学

  • システムゲノム科学

  • 分子生物学

研究キーワード 【 表示 / 非表示

  • RNAサイレンシング

  • エピゲノム

  • エピジェネティクス

  • クロマチン

  • ゲノム

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論文 【 表示 / 非表示

  • Clinical Utility of SARS-CoV-2 Whole Genome Sequencing in Deciphering Source of Infection

    Toshiki Takenouchi, Yuka W Iwasaki, Sei Harada, Hirotsugu Ishizu, Yoshifumi Uwamino, Shunsuke Uno, Asami Osada, Kodai Abe, Naoki Hasegawa, Mitsuru Murata, Toru Takebayashi, Koichi Fukunaga, Hideyuki Saya, Yuko Kitagawa, Masayuki Amagai, Haruhiko Siomi, Kenjiro Kosaki, Keio Donner Project

    Journal of Hospital Infection In press 2020年10月

    ISSN  0195-6701

     概要を見る

    COVID-19 caused by SARS-CoV-2 is a worldwide problem. From the standpoint of hospital infection control, determining the source of infection is critical. We conducted the present study to evaluate the efficacy of using whole genome sequencing to determine the source of infection in hospitalized patients who do not have a clear infectious contact history. Recently, we encountered two seemingly separate COVID-19 clusters in a tertiary hospital. Whole viral genome sequencing distinguished the two clusters according to the viral haplotype. However, the source of infection was unclear in 14 patients with COVID-19 who were clinically unlinked to clusters #1 or #2. These patients, who had no clear history of infectious contact within the hospital (“undetermined source of infection”), had haplotypes similar to those in cluster #2 but did not have two of the mutations used to characterize cluster #2, suggesting that these 14 cases of “undetermined source of infection” were not derived from cluster #2. Whole viral genome sequencing can be useful for confirming that sporadic COVID-19 cases with an undetermined source of infection are indeed not part of clusters at the institutional level.

  • Nuclear RNA export factor variant initiates piRNA‐guided co‐transcriptional silencing

    Murano K, Iwasaki YW, Ishizu H, Mashiko A, Shibuya A, Kondo S, Adachi S, Suzuki S, Saito K, Natsume T, Siomi MC, Siomi H

    the EMBO journal e102870   e102870 2019年08月

    ISSN  1460-2075

     概要を見る

    The PIWI-interacting RNA (piRNA) pathway preserves genomic integrity by repressing transposable elements (TEs) in animal germ cells. Among PIWI-clade proteins in Drosophila, Piwi transcriptionally silences its targets through interactions with cofactors, including Panoramix (Panx) and forms heterochromatin characterized by H3K9me3 and H1. Here, we identified Nxf2, a nuclear RNA export factor (NXF) variant, as a protein that forms complexes with Piwi, Panx, and p15. Panx-Nxf2-P15 complex formation is necessary in the silencing by stabilizing protein levels of Nxf2 and Panx. Notably, ectopic targeting of Nxf2 initiates co-transcriptional repression of the target reporter in a manner independent of H3K9me3 marks or H1. However, continuous silencing requires HP1a and H1. In addition, Nxf2 directly interacts with target TE transcripts in a Piwi-dependent manner. These findings suggest a model in which the Panx-Nxf2-P15 complex enforces the association of Piwi with target transcripts to trigger co-transcriptional repression, prior to heterochromatin formation in the nuclear piRNA pathway. Our results provide an unexpected connection between an NXF variant and small RNA-mediated co-transcriptional silencing.

  • Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification

    Sadahiro, T., Isomi, M., Muraoka, N., Kojima, H., Haginiwa, S., Kurotsu, S., Tamura, F., Tani, H., Tohyama, S., Fujita, J., Miyoshi, H., Kawamura, Y., Goshima, N., Iwasaki, Y. W., Murano, K., Saito, K., Oda, M., Andersen, P., Kwon, C., Uosaki, H., Nishizono, H., Fukuda, K. and Ieda, M.

    Cell Stem Cell 23 ( 3 ) 382 - 395 e5 2018年09月

    ISSN  1875-9777

     概要を見る

    The mesoderm arises from pluripotent epiblasts and differentiates into multiple lineages; however, the underlying molecular mechanisms are unclear. Tbx6 is enriched in the paraxial mesoderm and is implicated in somite formation, but its function in other mesoderms remains elusive. Here, using direct reprogramming-based screening, single-cell RNA-seq in mouse embryos, and directed cardiac differentiation in pluripotent stem cells (PSCs), we demonstrated that Tbx6 induces nascent mesoderm from PSCs and determines cardiovascular and somite lineage specification via its temporal expression. Tbx6 knockout in mouse PSCs using CRISPR/Cas9 technology inhibited mesoderm and cardiovascular differentiation, whereas transient Tbx6 expression induced mesoderm and cardiovascular specification from mouse and human PSCs via direct upregulation of Mesp1, repression of Sox2, and activation of BMP/Nodal/Wnt signaling. Notably, prolonged Tbx6 expression suppressed cardiac differentiation and induced somite lineages, including skeletal muscle and chondrocytes. Thus, Tbx6 is critical for mesoderm induction and subsequent lineage diversification.

  • Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline piRNA biogenesis

    Nishida, K. M., Sakakibara, K., Iwasaki, Y. W., Yamada, H., Murakami, R., Murota, Y., Kawamura, T., Kodama, T., Siomi, H. and Siomi, M. C.

    Nature 555 ( 7695 ) 260 - 264 2018年03月

    ISSN  1476-4687

     概要を見る

    PIWI-interacting RNAs (piRNAs) are small regulatory RNAs that bind to PIWI proteins to control transposons and maintain genome integrity in animal germ lines. piRNA 3' end formation in the silkworm Bombyx mori has been shown to be mediated by the 3'-to-5' exonuclease Trimmer (Trim; known as PNLDC1 in mammals), and piRNA intermediates are bound with PIWI anchored onto mitochondrial Tudor domain protein Papi. However, it remains unclear whether the Zucchini (Zuc) endonuclease and Nibbler (Nbr) 3'-to-5' exonuclease, both of which have pivotal roles in piRNA biogenesis in Drosophila, are required for piRNA processing in other species. Here we show that the loss of Zuc in Bombyx had no effect on the levels of Trim and Nbr, but resulted in the aberrant accumulation of piRNA intermediates within the Papi complex, and that these were processed to form mature piRNAs by recombinant Zuc. Papi exerted its RNA-binding activity only when bound with PIWI and phosphorylated, suggesting that complex assembly involves a hierarchical process. Both the 5' and 3' ends of piRNA intermediates within the Papi complex showed hallmarks of PIWI 'slicer' activity, yet no phasing pattern was observed in mature piRNAs. The loss of Zuc did not affect the 5'- and 3'-end formation of the intermediates, strongly supporting the idea that the 5' end of Bombyx piRNA is formed by PIWI slicer activity, but independently of Zuc, whereas the 3' end is formed by the Zuc endonuclease. The Bombyx piRNA biogenesis machinery is simpler than that of Drosophila, because Bombyx has no transcriptional silencing machinery that relies on phased piRNAs.

  • Profiling Open Chromatin Structure in the Ovarian Somatic Cells Using ATAC-seq

    Murano, K., Iwasaki, Y. W. and Siomi, H.

    Methods Mol Biol 1680   165 - 177 2018年

    ISSN  1940-6029

     概要を見る

    The assay for transposase-accessible chromatin using sequencing (ATAC-seq) was recently established as a method to profile open chromatin, which overcomes the sample size limitations of the alternative methods DNase/MNase-seq. To investigate the role of Piwi in heterochromatin formation around transposable element loci, we have used ATAC-seq to examine chromatin accessibility at target transposable elements in a Drosophila cultured cell line, ovarian somatic cells (OSCs). In this chapter, we describe our method to profile open chromatin structure in OSCs using ATAC-seq.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

競争的資金等の研究課題 【 表示 / 非表示

  • 小分子RNAが制御するクロマチンポテンシャルと遺伝子発現

    2019年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 岩崎 由香, 新学術領域研究(研究領域提案型), 補助金,  代表

  • 小分子RNAによるクロマチン制御を介した生殖ゲノム維持機構の全体像

    2018年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 岩崎 由香, 基盤研究(B), 補助金,  代表

  • 非コード小分子RNAによるヘテロクロマチン形成メカニズム

    2017年04月
    -
    2019年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 岩崎 由香, 新学術領域研究(研究領域提案型), 補助金,  代表

  • 小分子RNAとクロマチン因子によるゲノムレベルの非自己認識機構

    2015年04月
    -
    2018年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 岩崎 由香, 若手研究(A), 補助金,  代表

  • 非コード小分子RNAによるエピゲノム制御の作動ダイナミクス

    2015年04月
    -
    2017年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 岩崎 由香, 新学術領域研究(研究領域提案型), 補助金,  代表

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担当授業科目 【 表示 / 非表示

  • 分子生物学Ⅱ

    2021年度

  • 分子生物学Ⅰ

    2021年度

  • ゲノム科学

    2021年度

  • 分子生物学Ⅱ

    2020年度

  • 分子生物学Ⅰ

    2020年度

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