Kato, Yasuhiro

写真a

Affiliation

Graduate School of Science and Technology ( Yagami )

Position

Project Senior Assistant Professor (Non-tenured)/Project Assistant Professor (Non-tenured)/Project Lecturer (Non-tenured)
Page Top ▲

Career 【 Display / hide 】

  • 2021.04
    -
    2024.03

    Keio University, Research Centers and Institutes Keio University Global Research Institute, 特任講師

  • 2024.04
    -
    Present

    Keio University, Graduate School of Science and Technology, Project Senior Assistant Professor,

Page Top ▲
 

Books 【 Display / hide 】

  • 植物細胞工学シリーズ、18巻、カリウムイオン輸送系と生理学的役割

    水谷昭文, 佐藤陽子, 松田信行, 加藤靖浩, 魚住信之, 秀潤社, 2003

    Scope: 植物細胞工学シリーズ、18巻、pp.40-47

Page Top ▲

Papers 【 Display / hide 】

  • Title: Identification of Redox State Based on the Difference in Solvation Dynamics.

    Yasuhiro Kato, Jelena Muncan, Yoshinori Hirano, Hiroko Yamamoto, Roumiana Tsenkova, Masato Yasui

    ChemistryOpen 14 ( 5 ) e202400278 2025.05

    　View Summary

    Oxidation-reduction (Redox) reactions are crucial for many biological processes, yet there is no method available to evaluate redox states in a non-invasive, continuous manner. Here we introduce a novel approach to distinguish between reduced and oxidized states of glutathione (GSH and GSSG, respectively) using aquaphotomics near-infrared (NIR) spectroscopy and multivariate analysis. We identified clear differences in NIR spectra reflecting not only glutathione itself, but different redox states of glutathione based on the spectral features of water molecular conformations interacting with the reaction site. Molecular dynamic simulations also revealed the difference in water molecule coordination and hydration numbers around the reaction site. This approach not only sheds light on the significance of water molecules in redox reactions but also enables non-destructive, continuous assessment of redox states, with potential applications for bioreactor optimization.

  • Aquaphotomics Reveals Subtle Differences between Natural Mineral, Processed and Aged Water Using Temperature Perturbation Near-Infrared Spectroscopy

    Yasuhiro Kato, Jelena Munćan, Roumiana Tsenkova, Dušan Kojić, Masato Yasui, Jing-Yu Fan, Jing-Yan Han

    Applied Sciences (MDPI AG)  11 ( 19 ) 9337 - 9337 2021.10

    Accepted

    　View Summary

    Current approaches to the quality control of water are unsatisfying due to either a high cost or the inability to capture all of the relevant information. In this study, near-infrared spectroscopy (NIRS) with aquaphotomics as a novel approach was assessed for the discrimination of natural, processed and aged water samples. Temperature perturbation of water samples was employed to probe the aqueous systems and reveal the hidden information. A radar chart named an aquagram was used to visualize and compare the absorbance spectral patterns of waters at different temperatures. For the spectra acquired at a constant temperature of 30 °C, the discrimination analysis of different water samples failed to produce satisfying results. However, under perturbation by increasing the temperature from 35 to 60 °C, the absorbance spectral pattern of different waters displayed in aquagrams revealed different, water-specific dynamics. Moreover, it was found that aged processed water changed with the temperature, whereas the same processed water, when freshly prepared, had hydrogen bonded structures unperturbed by temperature. In summary, the aquaphotomics approach to the NIRS analysis showed that the water absorbance spectral pattern can be used to describe the character and monitor dynamics of each water sample as a complex molecular system, whose behavior under temperature perturbation can reveal even subtle changes, such as aging and the loss of certain qualities during storage.

  • Expression and localization of aquaporin-4 in sensory ganglia

    Jungo Kato, Yoshiki Takai, Mariko Kato Hayashi, Yasuhiro Kato, Manami Tanaka, Yoshiro Sohma, Yoichiro Abe, Masato Yasui

    Biochemical and Biophysical Research Communications (Elsevier BV)  451 ( 4 ) 562 - 567 2014.09

    Accepted,  ISSN  0006-291X

  • Unprecedented Cell-Selection Using Ultra-Quick Freezing Combined with Aquaporin Expression

    Yasuhiro Kato, Takayuki Miyauchi, Youichiro Abe, Dušan Kojić, Manami Tanaka, Nana Chikazawa, Yuhki Nakatake, Shigeru B. H. Ko, Daisuke Kobayashi, Akihiro Hazama, Shoko Fujiwara, Tatsuya Uchida, Masato Yasui

    PLoS ONE (Public Library of Science (PLoS))  9 ( 2 ) e87644 - e87644 2014.02

    Accepted

  • Bioengineering technique by the freeze tolerance of water channel Aquaporin

    Yasuhiro Kato, Yoichiro Abe, Masato Yasui

    Development Kidney 23 ( 1 ) 23 - 27 2014

    Lead author

display all >>

Page Top ▲

Papers, etc., Registered in KOARA 【 Display / hide 】

Page Top ▲

Reviews, Commentaries, etc. 【 Display / hide 】

  • Cryopreservation for the cell selection using ultra-quick freezing

    Yasuhiro Kato, Takayuki Miyauchi, Youichiro Abe, Manami Tanaka, Masato Yasui

    JOURNAL OF PHARMACOLOGICAL SCIENCES 124   90P - 90P 2014

    ISSN  1347-8613

  • Investegated freezing methods from water dyanamics by Aquaporin

    Yasuhiro Kato, Manami Tanaka, Youichiro Abe, Masato Yasui

    JOURNAL OF PHARMACOLOGICAL SCIENCES 118   94P - 94P 2012

    ISSN  1347-8613

Page Top ▲

Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 水分子の可視化とグルタチオンの機能的構造解析

    2023.04
    -
    2026.03

    日本学術振興会, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

    　View Summary

    生体の7割を占める「水」はどのような役割をもつのか。溶媒としての「水分子」は、バイオマーカーと言われる溶質が特定の立体的構造を形成し、生物学的機能を発現する際の要である。つまり溶媒と溶質の相互関係を可視化することが出来れば、“なぜ生命体が「水」を 必要とするのか”の問いに答えられるのではないか。

  • アクアポリンによるiPS細胞から神経系細胞への分化制御と腫瘍化細胞の除去

    2015.04
    -
    2018.03

    日本学術振興会, 科学研究費助成事業, 基盤研究(C), No Setting

  • " Selection of differentiated neural stem cells from iPS cells and removal of undifferentiated cells by using ultra-quick freezing with aquaporin expression "

    2014.04
    -
    2016.03

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Yasui Masato, OKADA Yohei, KATO Yasuhiro, Grant-in-Aid for Challenging Exploratory Research, No Setting

    　View Summary

    Based on the finding that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4, we proposed a novel cell-selection method using ultra-quick freezing combined with AQP expression. In this project, we examined if the residual undifferentiated cells can be removed during differentiation from iPS cells to neural stem cells by the ultra-quick freezing since AQP4 expression is induced in neural stem cells. As expected, AQP4 positive cells were slightly concentrated. However, there was no difference, before and after the ultra-quick freezing, in terms of tumor formation after transferring of the neural stem cells into mouse testis tissues. Further optimal conditioning of AQP4 expression is required in neural stem cells for clinical application.

  • Cryopreservation for The Cell Selection Using Water Channels AQPs Combination with Ultra-quick Freezing

    2012.04
    -
    2015.03

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, YASUHIRO Kato, Grant-in-Aid for Young Scientists (B), No Setting

    　View Summary

    Here we show that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4. We next examine if the expression of AQP4 combined with ultra-quick freezing can bes used to select cells efficiently under freezing conditions where most cells are died. Finally we propose that this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in regenerative medicine as well as cell-based functional assays or drug screening protocols.

  • Multi Gas was recommended the individual tissue culture system for the microenvironment of Stem Cell

    2010
    -
    2011

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, KATO Yasuhiro, Grant-in-Aid for Young Scientists (B), No Setting

    　View Summary

    The aim of this research is provided that a hypoxia microenvironment is a key component of the human oral mucosa stem cell. Because, tissue culture plays an important role in cell biology by using the CO_2 incubator have been widely employed. But this conventional CO_2 incubator poses a structural problem if hypoxia tissue culture is to be performed. We proposed the individual tissue culture system with hypoxic atmosphere for the microenvironment of stem cell.

Page Top ▲

Works 【 Display / hide 】

  • Life water Bag「せおう水袋」 防災ガイドブック監修

    加藤 靖浩

    2012.03
    , 

    Other

Page Top ▲
 

Courses Taught 【 Display / hide 】

  • MUFG ENDOWED COURSE : REBUILDING OF "TRUST" TOWARDS SUSTAINABLE SOCIETY: "2040" AND BEYOND 2

    2023

  • MUFG ENDOWED COURSE : REBUILDING OF "TRUST" TOWARDS SUSTAINABLE SOCIETY: "2040" AND BEYOND 1

    2023

Page Top ▲