慶應義塾研究者情報データベース

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• ライフサイエンス / 分子生物学 （Molecular Biology）

• ライフサイエンス / 発生生物学 （Developmental Biology）

• ライフサイエンス / 神経科学一般 （神経科学一般）

研究テーマ 【 表示 ／ 非表示 】

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• 中枢神経系の発生と再生, 

  1983年

  -

  継続中

著書 【 表示 ／ 非表示 】

著書 【 表示 ／ 非表示 】

• 新世紀の精神科治療・第6巻

  '多田敬典,　岡野栄之', 2003年05月

  担当範囲: 219-229

• 脳神経科学

  '徳永暁憲,　岡野栄之', 2003年05月

  担当範囲: 167-176

• Chapter 18. Neuron Regeneration Using iPS Cells.

  岡野 栄之, Science Publishers, CRC Press, Boca Raton, FL,, 2013年

  担当範囲: 315-332

• Transplantation of neural stem cells for spinal cord regeneration.

  岡野 栄之, Springer-Verlag (Heidelberg, Germany), 2008年

• Functions of neural RNA-binding proteins Musashi and Hu in stem cells and neuronal differentiation.

  岡野 栄之, Research Signpost, Kerala, India,, 2008年

  担当範囲: 107-121

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• Disorders in myelination in the twitcher mutant

  Mikoshiba Katsuhiko, Fujishiro Masatoshi, Kohsaka Shinichi, Okano Hideyuki, Takamatsu Ken, Tsukada Yasuzo

  Neurochemical Research 10 （ 8 ） 1129 - 1141 1985年08月

  ISSN  0364-3190

  　概要を見る

  <p>The twitcher is an autosomal recessive mutant mouse characterized by absence of galactosylceramidase. The twitcher shows clinical and histological features similar to those of human Krabbe-type leukodystrophy. We here present the results of a neurochemical and immunohistochemical analysis of the twitcher. Electrophoretic analysis revealed that in the particulate fraction of the spinal cord, myelin basic proteins (MBP) and proteolipid protein were decreased, and in the sciatic nerve fibers, PO protein, X, Y and MBP were clearly decreased. 2′,3′-Cyclic nucleotide 3′-phosphodiesterase (CNPase) activities of the pallium cerebri, brain stem and spinal cord of the twitcher were about 20% less than those of the control. However, in the sciatic nerve, the activity was half that of the control. Immunohistochemical studies were carried out by means of antisera against MBP and CNPase. There were clear patches indicating both MBP- and CNPase-negative reactions in the white matter of the central nervous system from the twitcher. The reaction on the section of sciatic nerve fibers from the twitcher showed a positive reaction only in a very limited number of fibers with both MBP and CNPase antisera. A clear astrocytic hypertrophy was detected by the antiserum against glial fibrillary acidic protein (GFAP). Even in the grey matter of the cerebral cortex, strong GFAP-positive astrocytes were clearly observed. © 1985 Plenum Publishing Corporation.</p>

  • Access to Document (DOI)

• Molecular genetic analysis of myelin-deficient mice: shiverer mutant mice show deletion in gene(s) coding for myelin basic protein.

  1) Kimura M, Inoko H, Katsuki M, Ando, A, Sato T, Hirose T, Takashima H, Inayama S, Okano H, Takamatsu K, Mikoshiba K, Tsukada Y, Watanabe I.

  Journal of Neurochemistry 44   692 - 696 1985年

  研究論文（学術雑誌）, 共著, 査読有り

  　概要を見る

  The gene expression of myelin basic proteins (MBPs) in shiverer mutant mice was investigated by the Northern and Southern hybridization techniques. In the control mice RNA molecules from the brains which were about 2,300 nucleotides in length were hybridized to cDNA of 1.8 kb encoding for a mouse MBP, but RNA from the brains of 3-week-old shiverer mutant mice contained no detectable amount of MBP transcripts hybridizing to this probe. Moreover the shiverer mutant mice lost several restriction fragments that hybridized to the same probe in the control mice when each of the five restriction enzymes, i.e., HindIII, PstI, PvuII, AccI, and StuI, was used. These data suggest that the shiverer mutation may correspond to the deletion of a large portion of MBP exon(s) in the gene, and this deletion causes inefficient transcription leading to the depletion of MBPs in the myelin and the dysmyelination observed in these mice.

• P₄₀₀ protein characteristic to Purkinje cells and related proteins in cerebella from neuropathological mutant mice

  Mikoshiba K., Okano H., Tsukada Y.

  Developmental Neuroscience 7 （ 3 ） 179 - 187 1985年

  ISSN  0378-5866

  　概要を見る

  <p>P₄₀₀ protein has been found in the cerebellum and localizes to Purkinje cells. It is a glycoprotein and reacts with plant lectins. Autoradiographic patterns of the protein profiles of mutant mice cerebella after intracranial injections of ¹⁴C-leucine were analyzed. P₄₀₀ protein was one of the dominant proteins labeled in the control cerebellum. No incorporation of ¹⁴C-leucine to the position of P₄₀₀ protein was found in the nervous and pcd mutant mice where no Purkinje cells and no P₄₀₀ protein are present. P₄₀₀ protein in weaver cerebellum was labelled more intensely than that in the control. In the staggerer mice, where dendritic arborization of Purkinje cell is poor and no spines are formed on the dendrites, no P₄₀₀ protein was found and no ¹⁴C-leucine incorporation was observed to the position of P₄₀₀ protein, suggesting that staggerer is a mutation where no P₄₀₀ protein accumulated in the fractions being studied. P₄₀₀ protein was phosphorylated independently of the presence of Ca²⁺. Among the proteins of the isolated Purkinje cells, P₄₀₀ protein was one of the major proteins phosphorylated.</p>

• Immunohistochemical, biochemical and electron microscopic analysis of myelin formation in the central nervous system of myelin deficient (mld) mutant mice.

  Mikoshiba K., Okano H., Inoue Y., Fujishiro M., Takamatsu K., Lachapelle F., Baumann N., Tsukada Y.

  Brain Research 432 （ 1 ） 111 - 121 1987年09月

  ISSN  0006-8993

  　概要を見る

  <p>Myelin deficiency (mld) is an autosomal recessive mutation in mice and is considered to be allelic to the shiverer (shi) mutation. Mld mice are characterized by hypomyelination of the central nervous system (CNS). They show typical symptoms such as tremor, tonic convulsion and ataxic movement. Subcellular fractionation of the CNS revealed that the MBP bands were greatly decreased in the P2A (myelin) fraction and the total content of myelin basic protein (MBP) was much lower than that in the control in all parts of the CNS. Sections from mld mice were examined by immunohistochemical tests with MBP antiserum, and a mosaic expression of MBP was found in the myelin of the mld mice. Since the major dense line is considered to be composed mainly of MBP, we investigated the myelin of mld mice by electron microscopy and found that there were 3 types of myelin: (1) a normal type compact myelin with a major dense line, (2) a shiverer-type myelin with no major dense line, and (3) a mixed-type myelin, in which within a myelin lamella the major dense line abruptly changes to cytoplasm of oligodendrocytes.</p>

• Inefficient transcription of the myelin basic protein gene possibly causes hypomyelination in myelin-deficient mutant mice

  Okano H., Miura M., Moriguchi A., Ikenaka K., Tsukada Y., Mikoshiba K.

  Journal of Neurochemistry 48 （ 2 ） 470 - 476 1987年

  ISSN  0022-3042

  　概要を見る

  <p>A hereditary dysmyelination mutation, named myelin deficient (mld), is considered to be allelic to shiverer, a deletion mutation of the myelin basic protein (MBP) gene. The present study showed that MBP expression is greatly reduced in mld, but that it is still detectable. Northern blot analysis revealed that the pronounced decrease in the MBP level in mld resulted from a reduced mRNA level and was not caused by deletion of a large portion of the MBP gene as in shiverer. Southern blod studies with BamHI-digested chromosomal DNA suggested some part of the MBP gene, at least the 5'-portion, was duplicated in mld. These results indicated that the mld and shiverer mutations were different from each other, even though genetic allelism between the two was reconfirmed. We also examined the developmental pattern of the gene expression of MBP and that of another protein, myelin proteolipid protein (PLP), specifically expressed in the oligodendrocyte, in mld by RNA dot blot study. The mRNA level of MBP in mld was greatly reduced during the active myelination stages, gradually increasing and remaining constant in the later stages. The PLP-mRNA content in mld was almost normal (60-80% that of control) at any stage of development. All these findings imply that the primary defect in mld is due to reduced transcriptional activity of the MBP gene.</p>

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• ヒトiPS細胞より誘導した甲状腺細胞の甲状腺機能低下症への再生医療実現に向けて

  岡野, 栄之

  科学研究費補助金研究成果報告書 2019年

• 神経変性疾患における超早期タンパク蓄積寄与因子の解明

  岡野, 栄之

  科学研究費補助金研究成果報告書 2018年

• ヒトiPS細胞と霊長類モデルを用いた治療開発の基盤整備

  岡野, 栄之

  科学研究費補助金研究成果報告書 2018年

• 患者由来iPS細胞を用いたPendred症候群における甲状腺腫の解明

  岡野, 栄之

  科学研究費補助金研究成果報告書 2017年

• 疾患特異的iPS細胞を用いた悪性高熱症の発症機構の解明と新規診断法・治療法の開発

  岡野, 栄之

  科学研究費補助金研究成果報告書 2017年

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総説・解説等 【 表示 ／ 非表示 】

総説・解説等 【 表示 ／ 非表示 】

• Neuroethical Issues of the Brain/MINDS Project of Japan

  Sadato N., Morita K., Kasai K., Fukushi T., Nakamura K., Nakazawa E., Okano H., Okabe S.

  Neuron （Neuron）  101 （ 3 ） 385 - 389 2019年02月

  ISSN  08966273

  　概要を見る

  © 2019 Elsevier Inc. The Brain/MINDS project aims to further understand the human brain and neuropsychiatric disorders through “translatable” biomarkers. Here, we describe the neuroethical issues of the project that have arisen from clinical data collection and the use of biological models of neuropsychiatric disorders.

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• The power of synthetic biology for bioproduction, remediation and pollution control: The UN's Sustainable Development Goals will inevitably require the application of molecular biology and biotechnology on a global scale

  de Lorenzo V., Prather K., Chen G., O'Day E., von Kameke C., Oyarzún D., Hosta-Rigau L., Alsafar H., Cao C., Ji W., Okano H., Roberts R., Ronaghi M., Yeung K., Zhang F., Lee S.

  EMBO Reports （EMBO Reports）  19 （ 4 ）  2018年04月

  ISSN  1469221X

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• Japan Strengthens Regenerative Medicine Oversight

  Sipp D., Okano H.

  Cell Stem Cell （Cell Stem Cell）  22 （ 2 ） 153 - 156 2018年02月

  ISSN  19345909

  　概要を見る

  © 2018 Elsevier Inc. The Japanese government initiated sweeping reforms targeting regenerative medicine in 2014, accompanied by substantial investment into stem cell research and development. We survey the impact of these developments and discuss how the government is working to accelerate regenerative medicine while ensuring safety and efficacy. The Japanese government initiated sweeping reforms targeting regenerative medicine in 2014, accompanied by substantial investment into stem cell research and development. We survey the impact of these developments and discuss how the government is working to accelerate regenerative medicine while ensuring safety and efficacy.

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• Self-renewal of neural stem cells and CNS repair

  Okano Hideyuki

  'Pre-Meeting Workshop:Neural Stem Cell Biology and Potential Therapeutic Applicaiton ''American Society for Neurochemictry''' （'California, USA'） , 

  2003年05月

  , 

  口頭発表（招待・特別）

• Attempts toward the stem cell therapy for spinal cord injuries

  Okano Hideyuki

  IBRO World Congress of Neuroscience （Prague.Czech Republic） , 

  2003年07月

  , 

  口頭発表（招待・特別）

• Stem cells for neurodegenerative diseases

  Okano Hideyuki

  <Stem cells:From Genetics to Cell Therapy> An International Symposium sponsored by the Tore Nilsson and Marcus Wallenberg foundation （'Lund, Sweden'） , 

  2003年09月

  , 

  口頭発表（招待・特別）

• Regulation of NOTCH signaling during proliferation and differentiation of postembryonic neuroblasts

  'Toriya Masako, Nakao Keiko, Okano Hideyuki'

  2003 Cold Spring Harbor Meeting on Neurology of Drosophila （'New York, USA'） , 

  2003年10月

  , 

  ポスター発表

• tincar encodes a Drosophila novel transmembrane protein required for the morphogenesis of eye and the function of dorsal vessel

  'Hirota Yuki, Sawamoto Kazunobu, Ueda Ryu, Okano Hideyuki'

  New Horizons in Molecular Sciences and Systems: An Integrated Approach （'Nago, Japan'） , 

  2003年10月

  , 

  ポスター発表

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競争的研究費の研究課題 【 表示 ／ 非表示 】

競争的研究費の研究課題 【 表示 ／ 非表示 】

• ALS運動ニューロンの脆弱性回避の分子機構

  2020年04月

  -

  2024年03月

  文部科学省・日本学術振興会, 科学研究費助成事業, 岡野 栄之, 基盤研究(A), 補助金,  研究代表者

• ヒトiPS細胞と霊長類モデルを用いた治療開発の基盤整備

  2014年07月

  -

  2019年03月

  文部科学省・日本学術振興会, 科学研究費助成事業, 岡野　栄之, 新学術領域研究(研究領域提案型), 補助金,  研究代表者

Works 【 表示 ／ 非表示 】

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• 医学部国際交流委員会委員

  2003年10月

  -

  2005年09月

  , 

  その他

• 国際交流委員会委員

  2001年10月

  -

  2003年09月

  , 

  その他

• 学務委員会委員

  2001年10月

  -

  2003年09月

  , 

  その他

受賞 【 表示 ／ 非表示 】

受賞 【 表示 ／ 非表示 】

• Faculty Award for Internalization 2016 (Impact factor Most Outstanding Award)

  2016年, 慶應義塾大学

• Molecular Brain Award

  2016年, The Association for the Study of Neurons and Diseases (A.N.D.)

• 第51回ベルツ賞(1等賞)

  2014年, 日本ベーリンガーインゲルハイム, 幹細胞を用いた脊髄損傷の再生医療

• Stem Cell Innovator Award

  2013年, GeneExpression Systems & Apasani Research Conference USA

• The Johnson & Johnson Innovation Award

  2011年, 日本再生医療学会

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担当授業科目 【 表示 ／ 非表示 】

• 生理学Ⅱ

  2024年度

• 分子生物学Ⅱ

  2024年度

• メディカル・プロフェッショナリズムⅢ

  2024年度

• ＭＣＢ

  2024年度

• 生理学Ⅱ

  2023年度

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• MEP3

  慶應義塾

  2017年04月

  -

  2018年03月

  , 

  春学期, 講義

• 症例検討(幹細胞医学）

  慶應義塾

  2017年04月

  -

  2018年03月

  , 

  講義

• 生理学実習

  慶應義塾

  2017年04月

  -

  2018年03月

  , 

  通年, 実習・実験

• 幹細胞医学

  慶應義塾

  2017年04月

  -

  2018年03月

  , 

  通年, 講義

• 生理学Ⅱ

  慶應義塾

  2017年04月

  -

  2018年03月

  , 

  通年, 講義, 115人

  生理学概論　内分泌　

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