Ko, Shigeru

写真a

Affiliation

School of Medicine, Center for Preventive Medicine （Shinanomachi）

Position

Project Associate Professor (Non-tenured)

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Career 【 Display / hide 】

  • 1992.04
    -
    1993.03

    Komaki Municipal Hospital, Aichi, Japan, Resident

  • 1993.04
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    1994.03

    Komaki Municipal Hospital, Aichi, Japan, Internal Medicine, Staff doctor

  • 1993.04
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    1996.03

    Komaki Municipal Hospital, Aichi, Japan, Department of Gastroenterology, Doctor, staff

  • 1999.10
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    2000.09

    Nagoya University Hospital, Internal Medicine II, Staff

  • 2000.10
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    2003.03

    University of Texas, Southwestern medical center at Dallas,, Department of Physiology, Postdoctoral Fellow

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Academic Background 【 Display / hide 】

  • 1986.04
    -
    1992.03

    Nagoya University, School of Medicine

    University, Graduated

  • 1996.04
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    1999.09

    Nagoya University, Graduate School of Medicine

    Completed, Doctoral course

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Academic Degrees 【 Display / hide 】

  • 博士（医学）, Nagoya University, 1999.09

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Licenses and Qualifications 【 Display / hide 】

  • 日本内科学会認定内科医

  • 日本医師会認定産業医

  • 日本消化器内視鏡学会　関東支部評議員

  • 日本消化器内視鏡学会　認定医

  • 日本膵臓学会　学会評議員

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Research Areas 【 Display / hide 】

  • Life Science / Clinical pharmacy

  • Life Science / Physiology

  • Life Science / Gastroenterology

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Research Keywords 【 Display / hide 】

  • pancreas, Regeneration, Physiology, Cystic fibrosis, CFTR

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Papers 【 Display / hide 】

  • Functional characteristics of L1156F-CFTR associated with alcoholic chronic pancreatitis in Japanese

    Kondo Shiho, Fujiki Kotoyo, Ko Shigeru B H, Yamamoto Akiko, Nakakuki Miyuki, Ito Yasutomo, Shcheynikov Nikolay, Kitagawa Motoji, Naruse Satoru, Ishiguro Hiroshi

    American Journal of Physiology - Gastrointestinal and Liver Physiology 309 （ 4 ） G260 - G269 2015.08

    ISSN  0193-1857

    　View Summary

    <p>Although cystic fibrosis is rare in Japanese, measurement of sweat Cl&lt;sup&gt;–&lt;/sup&gt; has suggested mild dysfunction of cystic fibrosis transmembrane conductance regulator (CFTR) in some patients with chronic pancreatitis. In the present study, we have investigated the association of CFTR variants and chronic pancreatitis in Japanese and the functional characteristics of a Japanese- and pancreatitis-specific CFTR variant, L1156F. Seventy patients with alcoholic chronic pancreatitis, 18 patients with idiopathic chronic pancreatitis, and 180 normal subjects participated. All exons and their boundaries and promoter region of the CFTR gene were sequenced. Human embryonic kidney-293 cells were transfected with three CFTR variants (M470V, L1156F, and M470V+L1156F), and the protein expression was examined. Xenopus laevis oocytes were injected with the CFTR variants, and bicarbonate (HCO(formula presented)) transport activity was examined. CFPAC-1 cells were transfected with the CFTR variants and Cl&lt;sup&gt;–&lt;/sup&gt;/HCO(formula presented) exchange activity was examined. Six variants (E217G, I556V, M470V+L1156F, Q1352H, and R1453W) were identified in the coding region of the CFTR gene. Cystic fibrosis-causing mutations were not found. The allele frequencies of L1156F and Q1352H in alcoholic chronic pancreatitis (5.0 and 7.9%) were significantly (P ˂ 0.01) higher than those in normal subjects (0.6 and 1.9%). L1156F was linked with a worldwide CFTR variant, M470V. Combination of M470V and L1156F significantly reduced CFTR expression to ~60%, impaired CFTR-mediated HCO(formula presented)/Cl&lt;sup&gt;–&lt;/sup&gt; transport activity to 50–60%, and impaired CFTR-coupled Cl&lt;sup&gt;–&lt;/sup&gt;/HCO(formula presented) exchange activity to 20–30%. The data suggest that the Japanese-specific CFTR variant L1156F causes mild dysfunction of CFTR and increases the risk of alcoholic chronic pancreatitis in Japanese.</p>

  • Unprecedented cell-selection using ultra-quick freezing combined with aquaporin expression

    Kato Yasuhiro, Miyauchi Takayuki, Abe Youichiro, Kojić Dušan, Tanaka Manami, Chikazawa Nana, Nakatake Yuhki, Ko Shigeru B H, Kobayashi Daisuke, Hazama Akihiro, Fujiwara Shoko, Uchida Tatsuya, Yasui Masato

    PLoS ONE 9 （ 2 ）  2014.02

    　View Summary

    <p>Freezing is usually used for preservation and storage of biological samples; however, this process may have some adverse effects such as cell membrane damage. Aquaporin (AQP), a water channel protein, has been suggested to play some roles for cryopreservation although its molecular mechanism remains unclear. Here we show that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4. We next examine if the expression of AQP combined with ultra-quick freezing can be used to select cells efficiently under freezing conditions where most cells are died. CHO cells stably expressing AQP4 were exclusively selected from mixed cell cultures. Having identified the increased expression of AQP4 during ES cell differentiation into neuro-ectoderm using bioinformatics, we confirmed the improved survival of differentiated ES cells with AQP4 expression. Finally we show that CHO cells transiently transfected with Endothelin receptor A and Aqp4 were also selected and concentrated by multiple cycles of freezing/thawing, which was confirmed with calcium imaging in response to endothelin. Furthermore, we found that the expression of AQP enables a reduction in the amount of cryoprotectants for freezing, thereby decreasing osmotic stress and cellular toxicity. Taken together, we propose that this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in regenerative medicine as well as cell-based functional assays or drug screening protocols. © 2014 Kato et al.</p>

  • Amendment of the Japanese Consensus Guidelines for Autoimmune Pancreatitis, 2013 II. Extrapancreatic lesions, differential diagnosis

    Kawa Shigeyuki, Okazaki Kazuichi, Kamisawa Terumi, Kubo Keishi, Ohara Hirotaka, Hasebe Osamu, Fujinaga Yasunari, Irisawa Atsushi, Notohara Kenji, Ito Tetsuhide, Inui Kazuo, Irie Hiroyuki, Nishino Takayoshi, Nishimori Isao, Tanaka Shigeki, Nishiyama Toshimasa, Suda Koichi, Shiratori Keiko, Tanaka Masao, Shimosegawa Tooru, Yamamoto Hiroshi, Uchida Kazushige, Kanno Atsushi, Kubota Kensuke, Ko Shigeru, Sakagami Junichi, Shimizu Kyoko, Sugiyama Masanori, Tada Minoru, Nakazawa Takahiro, Nishino Hirokazu, Hamano Hideaki, Hirooka Yoshiki, Hirano Kenji, Masamune Atsushi, Masuda Atsuhiro, Mizuno Nobumasa, Yamaguchi Koji, Yoshida Hitoshi

    Journal of Gastroenterology 49 （ 5 ） 765 - 784 2014

    ISSN  0944-1174

  • Amendment of the Japanese Consensus Guidelines for Autoimmune Pancreatitis, 2013 I. Concept and diagnosis of autoimmune pancreatitis

    Okazaki Kazuichi, Kawa Shigeyuki, Kamisawa Terumi, Ito Tetsuhide, Inui Kazuo, Irie Hiroyuki, Nishino Takayoshi, Notohara Kenji, Nishimori Isao, Tanaka Shigeki, Nishiyama Toshimasa, Suda Koichi, Shiratori Keiko, Tanaka Masao, Shimosegawa Tooru, Kubo Keishi, Ohara Hirotaka, Irisawa Atsushi, Fujinaga Yasunari, Hasebe Osamu, Yamamoto Hiroshi, Uchida Kazushige, Kanno Atsushi, Kubota Kensuke, Ko Shigeru, Sakagami Junichi, Shimizu Kyoko, Sugiyama Masanori, Tada Minoru, Nakazawa Takahiro, Nishino Hirokazu, Hamano Hideaki, Hirooka Yoshiki, Hirano Kenji, Masamune Atsushi, Masuda Atsuhiro, Mizuno Nobumasa, Yamaguchi Koji, Yoshida Hitoshi

    Journal of Gastroenterology 49 （ 4 ） 567 - 588 2014

    ISSN  0944-1174

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    <p>Background: In response to the proposal of the international consensus diagnostic criteria (ICDC) for autoimmune pancreatitis (AIP) and the Japanese diagnostic criteria in 2011, the 2009 Japanese consensus guidelines for managing AIP required revision. Methods: Three committees [the professional committee for making clinical questions (CQs) and statements by Japanese specialists, the expert panelist committee for rating statements by the modified Delphi method, and the evaluating committee by moderators] were organized. Fifteen specialists for AIP extracted the specific clinical statements from 1,843 articles published between 1963 and 2012 (obtained from Pub Med and a secondary database, and developed the CQs and statements. The expert panel individually rated the clinical statements using a modified Delphi approach, in which a clinical statement receiving a median score greater than seven on a nine-point scale from the panel was regarded as valid. Results: The professional committee created 13 CQs and statements for the current concept and diagnosis of AIP, 6 for extra-pancreatic lesions, 6 for differential diagnosis, and 11 for treatment. Conclusion: After evaluation by the moderators, amendments to the Japanese consensus guidelines for AIP have been proposed for 2013. © 2014 Springer.</p>

  • Amendment of the Japanese Consensus Guidelines for Autoimmune Pancreatitis, 2013 III. Treatment and prognosis of autoimmune pancreatitis

    Kamisawa Terumi, Okazaki Kazuichi, Kawa Shigeyuki, Ito Tetsuhide, Inui Kazuo, Irie Hiroyuki, Nishino Takayoshi, Notohara Kenji, Nishimori Isao, Tanaka Shigeki, Nishiyama Toshimasa, Suda Koichi, Shiratori Keiko, Tanaka Masao, Shimosegawa Tooru, Kubo Keishi, Ohara Hirotaka, Irisawa Atsushi, Fujinaga Yasunari, Hasebe Osamu, Yamamoto Hiroshi, Uchida Kazushige, Kanno Atsushi, Kubota Kensuke, Ko Shigeru, Sakagami Junichi, Shimizu Kyoko, Sugiyama Masanori, Tada Minoru, Nakazawa Takahiro, Nishino Hirokazu, Hamano Hideaki, Hirooka Yoshiki, Hirano Kenji, Masamune Atsushi, Masuda Atsuhiro, Mizuno Nobumasa, Yamaguchi Koji, Yoshida Hitoshi

    Journal of Gastroenterology 49 （ 6 ） 961 - 970 2014

    ISSN  0944-1174

    　View Summary

    <p>The standard treatment for autoimmune pancreatitis (AIP) is steroid therapy, although some patients improve spontaneously. Indications for steroid therapy in AIP patients are symptoms such as obstructive jaundice, abdominal pain, back pain, and the presence of symptomatic extrapancreatic lesions. Prior to steroid therapy, obstructive jaundice should be managed by biliary drainage, and blood glucose levels should be controlled in patients with diabetes mellitus. The recommended initial oral prednisolone dose for induction of remission is 0.6 mg/kg/day, which is administered for 2-4 weeks. The dose is then tapered by 5 mg every 1-2 weeks, based on changes in clinical manifestations, biochemical blood tests (such as liver enzymes and IgG or IgG4 levels), and repeated imaging findings (US, CT, MRCP, ERCP, etc.). The dose is tapered to a maintenance dose (2.5-5 mg/day) over a period of 2-3 months. Cessation of steroid therapy should be based on the disease activity in each case. Termination of maintenance therapy should be planned within 3 years in cases with radiological and serological improvement. Re-administration or dose-up of steroid is effective for treating AIP relapse. Application of immunomodulatory drugs is considered for AIP patients who prove resistant to steroid therapy. The prognosis of AIP appears to be good over the short-term with steroid therapy. The long-term outcome is less clear, as there are many unknown factors, such as relapse, pancreatic exocrine or endocrine dysfunction, and associated malignancy. © 2014 Springer.</p>

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Establishment of disease model organoid by CRISPR/Cas9 method and the analysis method for mutations

    2016.04
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    2018.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Principal investigator

  • Regenerative mechanisms of pancreatic endocrine and exocrine cells

    2015.04
    -
    2018.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

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Awards 【 Display / hide 】

  • 研究奨励賞

    2010, 財団法人日本医師会, 膵内・外分泌細胞再生機構の解明と膵疾患治療への臨床応用

  • Best Poster Award

    2008.03, The International Pancreatic Research Forum

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Memberships in Academic Societies 【 Display / hide 】

  • 日本糖尿病学会

    　

  • 日本内科学会

    　

  • 日本消化器病学会

    　

  • 日本消化器内視鏡学会

    　

  • 日本膵臓学会

    　

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