Reviews, Commentaries, etc. - Shinotsuka, Takanori

Visualization of water dynamics in the brain using multimodal multiphoton imaging

篠塚崇徳, 小関泰之, 安井正人, 塗谷睦生

バイオイメージング 33 ( 2 )  2024

ISSN  1342-2634

Visualization of water dynamics in brain tissue using multiphoton multimodal imaging

Shinotsuka Takanori, Miyazawa Tsuyoshi, Karasawa Keiko, Ozeki Yasuyuki, Yasui Masato, Nuriya Mutsuo

Proceedings for Annual Meeting of The Japanese Pharmacological Society (Japanese Pharmacological Society)  96   2-B-O05-1 2022

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The flow of cerebrospinal fluid is considered to be a critical factor in the clearance of wastes in the brain. The dynamics of fluid has been investigated by several experimental methods such as fluorescence microscopy and magnetic resonance imaging. However, because probes used for these imagings are much larger compared to water itself, the dynamics of the fluid have been poorly understood. Here, we applied a multimodal multiphoton imaging system to the living brain tissue. Combining stimulated Raman scattering and two-photon fluorescent imaging, the system enables us to visualize spatiotemporal dynamics of deuterated water and fluorescent dyes simultaneously at a cellular level. We demonstrate that deuterated water diffuses faster than fluorescent dyes in the brain tissue. Detailed analysis reveals deuterated water rapidly exchanges inside and outside of cells, whereas fluorescent dyes only diffuse through extracellular spaces. Furthermore, we find that the dynamics of deuterated water is robust to changes under physiological and pathophysiological conditions; there is little change in the spatiotemporal dynamics of deuterated water during development and ischemia whereas fluorescent dyes are severely affected. Thus, our new approach reveals unique properties of the dynamics of the fluid in the living brain tissue.

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Astrocytic gap junctional networks suppress cellular damages in an in vitro model of ischemia

Takanori Shinotsuka, Masato Yasui, Mutsuo Nuriya

JOURNAL OF PHARMACOLOGICAL SCIENCES (JAPANESE PHARMACOLOGICAL SOC)  124   74P - 74P 2014

ISSN  1347-8613

てんかんモデル条件下におけるアストロサイトの足突起のバリア機能の変化

篠塚崇徳, 権藤麻子, 権藤麻子, 森田彩加, 森田彩加, 阿部陽一郎, 安井正人, 塗谷睦生, 塗谷睦生

日本薬理学会関東部会プログラム・要旨集 131st 2014

Astrocytic gap junctional networks reduce ischemic damages in vitro

SHINOTSUKA Takanori, NURIYA Mutsuo, YASUI Masato

日本神経化学会大会抄録集(Web) 56th 2013

りん脂質修飾ナノ粒子の制御とそのマウス脳内における特異的分布(Controlling and spacific brain distribution of phospholipids-modified nanoparticles)

篠塚 崇徳, 杉戸 雄四郎, 横田 理, 鈴木 健一郎, 武田 健

神経化学 ((一社)日本神経化学会)  49 ( 2-3 ) 602 - 602 2010.08

ISSN  0037-3796

りん脂質修飾ナノ粒子を用いたマウス脳神経の脂質代謝の分析(Analysis of neuronal lipid metabolism in mouse by phospholipids-modified nanoparticles)

杉戸 雄四郎, 篠塚 崇徳, 横田 理, 鈴木 健一郎, 武田 健

神経化学 ((一社)日本神経化学会)  49 ( 2-3 ) 602 - 602 2010.08

ISSN  0037-3796

酸化亜鉛ナノ粒子胎仔期曝露がドパミン神経系に及ぼす影響

岡田悠佳, 高橋雄大, 篠塚崇徳, 立花研, 武田健

日本薬学会年会要旨集 130th ( 3 )  2010

ISSN  0918-9823

酸化チタンナノ粒子胎仔期曝露が脳内モノアミン系に及ぼす影響

高橋雄大, 水尾圭祐, 岡田悠佳, 篠塚崇徳, 武田健

日本薬学会年会要旨集 129th ( 3 )  2009

ISSN  0918-9823