論文 - 井奥　洪二

Adhesion Behavior of Microorganisms Isolated from Soil on Hydroxyapatite and Other Materials

Kamitakahara M., Takahashi S., Yokoi T., Inoue C., Ioku K.

Applied Biochemistry and Biotechnology （Applied Biochemistry and Biotechnology）  187 （ 3 ） 984 - 993 2019年03月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  02732289

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© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The adhesion behavior of microorganisms on different materials was examined to obtain basic knowledge for designing support materials for microorganisms. The microorganisms were isolated from soil, and their adhesion behavior on hydroxyapatite (HA), carbon-coated HA (Carbon), poly (vinyl chloride) (PVC), and polyurethane (PU) pellets was investigated. The total metabolic activity on and adherence of microorganisms to the tested materials were in the following order: HA > Carbon > PVC > PU. This order was consistent with the extent of hydrophilicity of the materials. Morphological examination and polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis of microorganisms adhered to the materials revealed that the activities and states of microorganisms were affected by the composition of the pellets. PCR-DGGE analysis revealed various species of microorganisms adhered to the HA pellet. HA ceramics are expected to be one of the most suitable materials for supporting microorganisms.

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Preparation of spherical porous hydroxyapatite granules as support materials for microorganisms

Masanobu Kamitakahara, Shohei Takahashi, Taishi Yokoi, Chihiro Inoue, Koji Ioku

J. Ceram. Soc. Japan （The Ceram. Soc. Japan）  126 （ 9 ） 732 - 735 2018年08月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  18820743

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© 2018 The Ceramic Society of Japan. All rights reserved. Suitable support materials for microorganisms are useful for bioreactors to achieve high efficiencies. Spherical porous hydroxyapatite (HA) granules about 5 mm in diameter composed of rod-like particles were prepared through hydrothermal treatment. The granules with and without pores tens of micrometers in size were prepared. These granules were immersed in a suspension containing microorganisms obtained from the soil, and the adhesion of microorganisms on the granules was examined. The adhesion of microorganisms on the surfaces of the HA granules was observed. The total metabolic activity of the microorganisms adhering onto the granule with pores tens of micrometers in size was higher than that adhering onto the granule without such pores. These results suggested that the HA granules had an affinity for microorganisms, and that granules with pores tens of micrometers in size are expected to be support materials for microorganisms.

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Formation Process of Hydroxyapatite Granules in Agarose Hydrogel by Electrophoresis

Kenshiro Kimura, Masanobu Kamitakahara, Taishi Yokoi, Koji Ioku

Cryst. Growth Des. （Elsevier）  18 （ 4 ） 1961 - 1966 2018年03月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  15287483

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© 2018 American Chemical Society. Biomimetic mineralization is useful in obtaining organic-inorganic composite and inorganic materials with controlled sizes and morphologies. The formation of hydroxyapatite (HA) granules with porous structures can be achieved by using electrophoresis processes inside an agarose gel in which the compulsory migration of calcium and phosphate ions are driven by an electric field, causing precipitation of the granules. In the present study, we examined the formation process of HA granules through electrophoresis and the subsequent aging process. Spherical particles of amorphous calcium phosphate (ACP) precipitated, increasing their size with increased running time during electrophoresis; then the ACP granules gradually transformed to the HA granules during the aging process in ultrapure water. When the ACP granules were large, one HA granule was formed from one ACP granule. One HA granule was formed from multiple ACP granules for small-sized ACP granules. The size of ACP granules depends on the gel concentration and pH in the electrophoresis. The dominant transformation process from ACP to HA was the dissolution-precipitation process.

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Adhesion behaviors of Escherichia coli on hydroxyapatite

Masanobu Kamitakahara, Shohei Takahashi, Taishi Yokoi, Chihiro Inoue, Koji Ioku

Mater. Sci. Eng. C （Elsevier）  61   169 - 173 2016年04月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  0928-4931

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Optimum design of support materials for microorganisms is required for the construction of bioreactors. However, the effects of support materials on microorganisms are still unclear. In this study, we investigated the adhesion behavior of Escherichia coli (E. coli) on hydroxyapatite (HA), polyurethane (PU), poly(vinyl chloride) (PVC), and carbon (Carbon) to obtain basic knowledge for the design of support materials. The total metabolic activity and number of E. coli adhering on the samples followed the order of HA ≈ Carbon > PVC > PU. On the other hand, the water contact angle of the pellet surfaces followed the order of HA < Carbon < PVC < PU. The high hydrophilicity of the HA surface might be one of the suitable characters for the adhesion of E. coli. The results implied that HA has a potential as a support material for microorganisms used in bioreactors.

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Effect of silicate incorporation on in vivo responses of α-tricalcium phosphate ceramics

Masanobu Kamitakahara, Eri Tatsukawa, Yasuaki Shibata, Shota Umemoto, Taishi Yokoi, Koji Ioku, Tohru Ikeda

J Mater Sci: Mater Med （Springer）  27 （ 97 ） 971 - 979 2016年03月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  0957-4530 (Print) 1573-4838 (Online)

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In addition to calcium phosphate-based ceramics, glass-based materials have been utilized as bone substitutes, and silicate in these materials has been suggested to contribute to their ability to stimulate bone repair. In this study, a silicate-containing α-tricalcium phosphate (α-TCP) ceramic was prepared using a wet chemical process. Porous granules composed of silicate-containing α-TCP, for which the starting composition had a molar ratio of 0.05 for Si/(P + Si), and silicate-free α-TCP were prepared and evaluated in vivo. When implanted into bone defects that were created in rat femurs, α-TCP ceramics either with or without silicate were biodegraded, generating a hybrid tissue composed of residual ceramic granules and newly formed bone, which had a tissue architecture similar to physiological trabecular structures, and aided regeneration of the bone defects. Supplementation with silicate significantly promoted osteogenesis and delayed biodegradation of α-TCP. These results suggest that silicate-containing α-TCP is advantageous for initial skeletal fixation and wound regeneration in bone repair.

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Spherical porous hydroxyapatite granules containing composites of magnetic and hydroxyapatite nanoparticles for the hyperthermia treatment of bone tumor

Masanobu Kamitakahara, Naohiro Ohtoshi, Masakazu Kawashita, Koji Ioku

J Mater Sci: Mater Med （Springer）  27 （ 93 ） 931 - 937 2016年03月

研究論文（学術雑誌）, 共著, 査読有り

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Hydrothermal-assisted biomimetic synthesis of brush-like Hap/PAN composite and its application for decontaminating the metal ions

Hehe Guo, Huaijuan Zhou, Ping Jin, Wenjing Li, Yining Ma, IOKU Koji, Shidong Ji

RSC Advances （Royal Society of Chemistry）  6 （ 7 ） 5965 - 5972 2016年01月

研究論文（学術雑誌）, 共著, 査読有り

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Carbonate-containing hydroxyapatite synthesized by the hydrothermal treatment of different calcium carbonates in a phosphate-containing solution

Masanobu Kamitakahara, Takuya Nagamoria, Taishi Yokoia, IOKU Koji

J. Asian Ceram. Soc. （Elsevier）  3   287 - 291 2015年05月

研究論文（学術雑誌）, 共著, 査読有り

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Effect of preparation temperature on the ability of bone char to remove fluoride ion and organic contaminants

Sota TERASAKA, Masanobu KAMITAKAHARA, Taishi YOKOI, IOKU Koji

J. Ceram. Soc. Japan 122 （ 12 ） 995 - 999 2014年12月

研究論文（学術雑誌）, 共著, 査読有り

バイオセラミックスとしてのリン酸カルシウム多孔体

井奥 洪二、上高原　理暢

Phosphorus Letter （日本無機リン化学会）   （ 81 ） 17 - 26 2014年10月

研究論文（学術雑誌）, 共著, 査読有り

医療分野におけるバイオインスパイアード材料の応用

井奥 洪二、横井 太史、上高原　理暢

セラミックス （日本セラミックス協会）  49 （ 5 ） 397 - 401 2014年05月

研究論文（学術雑誌）, 共著, 査読有り

Importance of nucleation in transformation of octacalcium phosphate to hydroxyapatite

Natsuko Ito, Masanobu Kamitakahara, Masahiro Yoshimura, Koji Ioku

Materials Science and Engineering C （Elsevier）  40   121 - 126 2014年04月

研究論文（学術雑誌）, 共著, 査読有り

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Preparation and evaluation of spherical porous granules of octacalcium phosphate/hydroxyapatite as drug carriers in bone cancer treatment

Natsuko Ito, Masanobu Kamitakahara, Koji Ioku

Materials Letters  （ 120 ） 94-96 2014年02月

研究論文（学術雑誌）, 共著, 査読有り

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Promotion of normal healing of bone defects under estrogen deficiency by implantation of beta-tricalcium phosphate composed of rod-shaped particles

Eri Tatsukawa, Yoshinori Gonda, Masanobu Kamitakahara, Masaaki Matsuura, Masaru Ushijima, Yasuaki Shibata, Ikuho Yonezawa, Mutsunori Fujiwara, Koji Ioku, Tohru Ikeda1

J. Orthopaedic Res. 32   189 - 196 2014年02月

研究論文（学術雑誌）, 共著, 査読有り

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A bone substitute with high affinity for vitamin D-binding protein ― relationship with niche of osteoclasts

Tohru Ikeda, Michiyuki Kasai, Eri Tatsukawa, Masanobu Kamitakahara, Yasuaki Shibata, Taishi Yokoi, Takayuki K. Nemoto, IOKU Koji

J. Cell. Mol. Med. 18 （ 1 ） 170-180 2014年01月

研究論文（学術雑誌）, 共著, 査読有り

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Behavior of osteoblast-like cells on calcium-deficient hydroxyapatite ceramics composed of particles with different shapes and sizes

KAMITAKAHARA Masanobu, UNO Yuika, IOKU Koji

J Mater Sci: Mater Med （Springer）  25   239 - 245 2014年01月

研究論文（学術雑誌）, 共著, 査読有り

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Preparation and evaluation of spherical Ca-deficient hydroxyapatite granules with controlled surface microstructure as drug carriers

Masanobu Kamitakahara, Ryohei Imai,Koji Ioku

Materials Science and Engineering C 33   2446 - 2450 2013年03月

研究論文（学術雑誌）, 共著, 査読有り

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Observation of Transformation Behavior of Octacalcium Phosphate to Hydroxyapatite

Nasuko Ito, Masanobu Kamitakahara, Koji Ioku

Key Eng. Mater. 529-530   11-14 2013年01月

研究論文（学術雑誌）, 共著, 査読有り

Characterization and In Vitro Evaluation of Silicate-Containing Tricalcium Phosphate Prepared through Wet Chemical Process

Masanobu Kamitakahara, Shota Umemoto, Koji Ioku

Key Eng. Mater. 529-530   105-108 2013年01月

研究論文（学術雑誌）, 共著, 査読有り

Production of tubular porous hydroxyapatite using electrophoretic deposition

Cem Bulent USTUNDAG, Figen KAYA, Masanobu KAMITAKAHARA, Cengiz KAYA, IOKU Koji

J. Ceram. Soc. Japan 120 （ 12 ） 569-573 2012年12月

研究論文（学術雑誌）, 共著, 査読有り